Can you explain lane and eynon method, Biology

Assignment Help:

Q. Can you explain Lane and Eynon method?

Reducing sugars are those, which have free sugar groups (e.g. glucose, fructose etc.) and hence may be estimated directly by titrating the solution of the sample with Fehling's solution. Total sugars include both reducing and non-reducing sugars. Non-reducing sugars (e.g. maltose, lactose, sucrose etc.) do not contain free sugar groups and cannot reduce Fehling solution. Hence, non-reducing sugars must be hydrolyzed to monosaccharides by heating with acid before titration.

Reducing sugars are acted upon by the alkali of the Fehling solution to form enediols. These enediols are very unstable and reactive and they reduce Cu²+ ions to Cu+ ions.

These Cu+ ions combine with hydroxyl groups to form cuprous hydroxide, which on heating gives red precipitate of cuprous oxide. To get a sharp end point, methylene blue is added which is reduced to a colourless leuco compound restoring the red colour of the solution. Sodium potassium tartarate keeps the Cu²+ ions in the solution, thus ensuring a continuous supply of Cu²+ ions for reduction.


Related Discussions:- Can you explain lane and eynon method

Explain the five kingdom systems, The five kingdom systems To cope up w...

The five kingdom systems To cope up with above discussed problem a number of alternative classificatory schemes have been suggested with more than two kingdoms. The one which h

How age affects the bmr, How Age affects the BMR? The BMR per unit weig...

How Age affects the BMR? The BMR per unit weight also varies with age, being higher in children and lower in the elderly. The loss of FFM with ageing is associated with a decli

Objective of the radiographic technique, Objective of the Radiographic Tech...

Objective of the Radiographic Technique The ball bearings are opaque and their diameter have to be measured with the help of vernier caliper prior to use. (2 to 5 mm diameter b

Absorption of amino acids and peptides, Absorption of amino acids and  pep...

Absorption of amino acids and  peptides Generally,  the  dietary proteins are almost comp!etely  digested to their constituent amino acids and these are rapidly absorbed  from

Role of biotic factors inducing senescence, Role of Biotic Factors Inducing...

Role of Biotic Factors Inducing Senescence Besides environmental and endogenous factors, biotic factors also play a role in inducing senescence. For example, due to an attack

Hard paste removal-therma-fill, Hard paste removal -    Hard setting Ca...

Hard paste removal -    Hard setting Ca(Ho) 2 paste (like Endocal 10) can be softened by EDTA and removed by file or microdebrider. -    Care must be taken not to gouge or

Explain the use and care of microscope, Explain the Use and Care of Microsc...

Explain the Use and Care of Microscope? Proper care and maintenance of microscope is needed. Following points should be kept in mind while handling the microscope: (i) Instr

Explain congenital syphilis, Congenital Syphilis  A positive serologica...

Congenital Syphilis  A positive serological test for syphilis in a newborn without stigmata of syphilis may be due either to passive transfer of maternal antibodies or to prena

Respiratory system in living organisms, Respiratory System in Living Organi...

Respiratory System in Living Organisms Respiration is a necessary physiological process in all living organisms by which they obtain energy for carrying out all the metabolic

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd