Can you explain lane and eynon method, Biology

Assignment Help:

Q. Can you explain Lane and Eynon method?

Reducing sugars are those, which have free sugar groups (e.g. glucose, fructose etc.) and hence may be estimated directly by titrating the solution of the sample with Fehling's solution. Total sugars include both reducing and non-reducing sugars. Non-reducing sugars (e.g. maltose, lactose, sucrose etc.) do not contain free sugar groups and cannot reduce Fehling solution. Hence, non-reducing sugars must be hydrolyzed to monosaccharides by heating with acid before titration.

Reducing sugars are acted upon by the alkali of the Fehling solution to form enediols. These enediols are very unstable and reactive and they reduce Cu²+ ions to Cu+ ions.

These Cu+ ions combine with hydroxyl groups to form cuprous hydroxide, which on heating gives red precipitate of cuprous oxide. To get a sharp end point, methylene blue is added which is reduced to a colourless leuco compound restoring the red colour of the solution. Sodium potassium tartarate keeps the Cu²+ ions in the solution, thus ensuring a continuous supply of Cu²+ ions for reduction.


Related Discussions:- Can you explain lane and eynon method

Texture of food product, Q. Texture of food product? Texture may be acc...

Q. Texture of food product? Texture may be accessed through touch or mouth feels. When the food is placed in the mouth, the surface of the tongue and other sensitive skin react

Infectious bronchitis (ib), I nfectious bronchitis (IB) Infectious bro...

I nfectious bronchitis (IB) Infectious bronchitis is a highly contagious respiratory disease of poultry, caused by a virus belonging to the family Coronaviridae. Its existence

Avian infectious bronchitis (ib), A v i a n infectious bronchitis (IB) ...

A v i a n infectious bronchitis (IB) An acute, highly contagious respiratory disease of chicken caused by a member of the family Coronaviridae, IB was first recognized in I

What are the physical signs of aortic stenosis, Q. What are the Physical Si...

Q. What are the Physical Signs of aortic stenosis? Typically the carotid pulse in severe aortic stenosis is delayed (pulsus tardus). It is also decreased in amplitude (pulses p

Grazing food chain - food chain, Grazing Food Chain - Food Chain Grazi...

Grazing Food Chain - Food Chain Grazing food chains are quite familiar to most of us. Cow or deer grazing in a field represent a grazing food chain. Similarly, eating of phyto

Plant classification according to ancient greeks and romans, Q. Plant class...

Q. Plant classification according to Ancient Greeks and Romans? Hippocrates, "The Father of Medicine" (460-377 B.C.) is reputed to have been one of Democritus 's disciples. He

Biochemical, Explain the salient features biochemical origin of life.

Explain the salient features biochemical origin of life.

Determine the effect of drug on the blood pressure of rats, A scientist wan...

A scientist wanted to study the effect of a drug on the blood pressure of rats. She set up an experiment in which the experimental group consisted of rats that were injected with a

Explain digestion and of digestive system of platyhelminthes, Q What are th...

Q What are the kinds of digestion and of digestive system of platyhelminthes? Flatworms have incomplete digestive systems and they present intracellular and extracellular compl

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd