Can you explain lane and eynon method, Biology

Assignment Help:

Q. Can you explain Lane and Eynon method?

Reducing sugars are those, which have free sugar groups (e.g. glucose, fructose etc.) and hence may be estimated directly by titrating the solution of the sample with Fehling's solution. Total sugars include both reducing and non-reducing sugars. Non-reducing sugars (e.g. maltose, lactose, sucrose etc.) do not contain free sugar groups and cannot reduce Fehling solution. Hence, non-reducing sugars must be hydrolyzed to monosaccharides by heating with acid before titration.

Reducing sugars are acted upon by the alkali of the Fehling solution to form enediols. These enediols are very unstable and reactive and they reduce Cu²+ ions to Cu+ ions.

These Cu+ ions combine with hydroxyl groups to form cuprous hydroxide, which on heating gives red precipitate of cuprous oxide. To get a sharp end point, methylene blue is added which is reduced to a colourless leuco compound restoring the red colour of the solution. Sodium potassium tartarate keeps the Cu²+ ions in the solution, thus ensuring a continuous supply of Cu²+ ions for reduction.


Related Discussions:- Can you explain lane and eynon method

Atp molecules are produced for each glucose molecule, How many ATP molecule...

How many ATP molecules are produced for each glucose molecule used in fermentation? How many ATP molecules are produced for each glucose molecule used in aerobic respiration? I

Define agar syringe or agar sausage method, Define Agar Syringe/Agar Sausag...

Define Agar Syringe/Agar Sausage Method? Agar syringe method involves 100 ml syringe, which is filled with agar. A layer of agar is pushed beyond the end of the barrel by means

Biological sickness of soil, what is biological sickness of soil and its ma...

what is biological sickness of soil and its management

Determine general principles of allergy management, Determine General Princ...

Determine General Principles of Allergy Management? The four general principles of allergy management include: 1.  Avoid factors that cause symptoms. 2.  Use appropriate

Suicide cells, significant role of suicide cells?

significant role of suicide cells?

What are some human diseases caused by virus, What are some human diseases ...

What are some human diseases caused by virus and what are their respective modes of transmission? The main viral diseases transmitted by respiratory secretions (cough, sneezes)

What do you understand by term - acanthus, What do you understand by term -...

What do you understand by term - acanthus The egg and larval phase of an acanthocephalan which passes from the female parasite to the feces of host.

Describe Fatty acids, In chemistry and particularly in biochemistry the fat...

In chemistry and particularly in biochemistry the fatty acid is a carboxylic acid with a long aliphatic tail (chain) that is either saturated / unsaturated. Many obviously occurrin

Define soy protein concentrates, Define Soy Protein Concentrates? The A...

Define Soy Protein Concentrates? The Association of American Feed Control Officials, Inc. (AAFCO) specifies soy protein concentrates as follows: "Soy Protein Concentrate is pre

Hypocoagulation disorder and vitamin k deficiency, Which of the following p...

Which of the following patients likely faces the highest risk of an acquired hypocoagulation disorder and vitamin K deficiency? Answer A. A patient who is immunocompromised as a re

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd