Can you explain lane and eynon method, Biology

Assignment Help:

Q. Can you explain Lane and Eynon method?

Reducing sugars are those, which have free sugar groups (e.g. glucose, fructose etc.) and hence may be estimated directly by titrating the solution of the sample with Fehling's solution. Total sugars include both reducing and non-reducing sugars. Non-reducing sugars (e.g. maltose, lactose, sucrose etc.) do not contain free sugar groups and cannot reduce Fehling solution. Hence, non-reducing sugars must be hydrolyzed to monosaccharides by heating with acid before titration.

Reducing sugars are acted upon by the alkali of the Fehling solution to form enediols. These enediols are very unstable and reactive and they reduce Cu²+ ions to Cu+ ions.

These Cu+ ions combine with hydroxyl groups to form cuprous hydroxide, which on heating gives red precipitate of cuprous oxide. To get a sharp end point, methylene blue is added which is reduced to a colourless leuco compound restoring the red colour of the solution. Sodium potassium tartarate keeps the Cu²+ ions in the solution, thus ensuring a continuous supply of Cu²+ ions for reduction.


Related Discussions:- Can you explain lane and eynon method

Economic significance - conservation of wildlife, Economic Significance - C...

Economic Significance - Conservation of Wildlife Certain wild species are important because of their actual or potential economic value to people. Wildlife resources provide p

Animal cloning, Animal Cloning: When entire animals are obtained from s...

Animal Cloning: When entire animals are obtained from somatic cells of an animal, it is known as animal cloning. Cloning is usual in plants but in case of animals only some deg

Reviews - assurance engagements, Reviews - Assurance engagements The o...

Reviews - Assurance engagements The objective of financial statements of a review is to enable an auditor to state where, on the essential of procedures that do not give all t

Estimate the molar mass of protein, Estimate the molar mass of protein? ...

Estimate the molar mass of protein? An aqueous solution of the protein bovine serum albumin, containing 2:00 *10 -2 g of protein per cubic centimeter, has an osmotic pressure

Explain vernalisation, Name the state in humans in which the blood choleste...

Name the state in humans in which the blood cholesterol content becomes abnormally high. a) Explain vernalisation. b) Many villagers near Industrial area vary from "blue bab

Compound feed prepration, Compound feed prepration The compounding of anim...

Compound feed prepration The compounding of animal feed involves processing and blending of raw feed ingredients of wide ranging physical, chemical and nutritional composition int

What is condition for the mendel''s second law to be valid, What is conditi...

What is condition for the Mendel's second law to be valid? The Mendel's second law is only valid for genes located in various chromosomes. For genes situated in the similar chr

An a=t mispairing leads to an a=c substitution, An A=T mispairing leads to ...

An A=T mispairing leads to an A=C substitution. The other DNA helix will contain a(n) __ pair. a. A=C b. A=T c. G=C d. B=Q e. T=T Can you also explain it please so if I'm ask

What are the in-vivo imaging techniques, What are the in-vivo imaging techn...

What are the in-vivo imaging techniques The first of the in-vivo imaging techniques, computer tomography (CT) scanning, came on stream in the early 1970s. As technologies devel

What are cell movements, What are cell movements? How are these movements c...

What are cell movements? How are these movements created? Cell movements are movements done by cell structures, like the movements of cilia and flagella, the pseudopod movement

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd