Can you explain lane and eynon method, Biology

Assignment Help:

Q. Can you explain Lane and Eynon method?

Reducing sugars are those, which have free sugar groups (e.g. glucose, fructose etc.) and hence may be estimated directly by titrating the solution of the sample with Fehling's solution. Total sugars include both reducing and non-reducing sugars. Non-reducing sugars (e.g. maltose, lactose, sucrose etc.) do not contain free sugar groups and cannot reduce Fehling solution. Hence, non-reducing sugars must be hydrolyzed to monosaccharides by heating with acid before titration.

Reducing sugars are acted upon by the alkali of the Fehling solution to form enediols. These enediols are very unstable and reactive and they reduce Cu²+ ions to Cu+ ions.

These Cu+ ions combine with hydroxyl groups to form cuprous hydroxide, which on heating gives red precipitate of cuprous oxide. To get a sharp end point, methylene blue is added which is reduced to a colourless leuco compound restoring the red colour of the solution. Sodium potassium tartarate keeps the Cu²+ ions in the solution, thus ensuring a continuous supply of Cu²+ ions for reduction.


Related Discussions:- Can you explain lane and eynon method

Meiosis, How do meiosis do in developing of new varieties of crops

How do meiosis do in developing of new varieties of crops

Appendical skeleton, it consists of the girdles and the skeleton of the lim...

it consists of the girdles and the skeleton of the limbs

Primitive arthropods, Primitive Arthropods The primitive arthropods, O...

Primitive Arthropods The primitive arthropods, Onychophora (for example Peripatus) have a series of paired legs which are not jointed but have a ringed appearance because of t

Describe the general guidelines for use of bone grafting, General guideline...

General guidelines for use of BONE GRAFTING 1. The surgical site should be prepared, that is it should be free of granulation tissue. 2. For autogenous grafts- the donor sit

Action of natural selection in changing environments, Directional or progre...

Directional or progressive selection is the mode of selection associated with environmental change. We said earlier that in an unchanging environment stabilising selection removes

Plant cells differ from animal cells, State the ways in which (a) all...

State the ways in which (a) all plant cells, (b) some plant cells vary from animal cells.    (a) All plant cells have a cellulose cell wall.  (b) Some plant c

Types of cells in phloem, Types of cells in Phloem The phloem consists...

Types of cells in Phloem The phloem consists of 4 types of cells: i).sieve elements, ii) companion cells, iii) phloem parenchyma and sometimes iv) phloem fibres. The metabolit

Analysis of different milk samples, could you please give me a data in the ...

could you please give me a data in the form of tabular column based on milk analysis which can be done in home itself?

Process of memory, Process of Memory: There are three stages of memory E...

Process of Memory: There are three stages of memory Encoding process: It is the process of receiving sensory input and transforming it into a form or code, which can be stored.

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd