Can you explain lane and eynon method, Biology

Assignment Help:

Q. Can you explain Lane and Eynon method?

Reducing sugars are those, which have free sugar groups (e.g. glucose, fructose etc.) and hence may be estimated directly by titrating the solution of the sample with Fehling's solution. Total sugars include both reducing and non-reducing sugars. Non-reducing sugars (e.g. maltose, lactose, sucrose etc.) do not contain free sugar groups and cannot reduce Fehling solution. Hence, non-reducing sugars must be hydrolyzed to monosaccharides by heating with acid before titration.

Reducing sugars are acted upon by the alkali of the Fehling solution to form enediols. These enediols are very unstable and reactive and they reduce Cu²+ ions to Cu+ ions.

These Cu+ ions combine with hydroxyl groups to form cuprous hydroxide, which on heating gives red precipitate of cuprous oxide. To get a sharp end point, methylene blue is added which is reduced to a colourless leuco compound restoring the red colour of the solution. Sodium potassium tartarate keeps the Cu²+ ions in the solution, thus ensuring a continuous supply of Cu²+ ions for reduction.


Related Discussions:- Can you explain lane and eynon method

Spina bifida cystica, Spina Bifida Cystica There is a protrusion of me...

Spina Bifida Cystica There is a protrusion of meninges and spinal cord a) Meningocele- it is relatively uncommon lesion (4-5 per cent). In this there is protrusion of me

Disadvantage of a monoculture, (a)What do you understand by the term 'monoc...

(a)What do you understand by the term 'monoculture'? (b) What is one disadvantage of a monoculture?   a) 'Monoculture' is the term applied to the growing

What is bidirectional glenn without cardio pulmonary bypass, What is Bidire...

What is Bidirectional Glenn (BDG) without Cardio Pulmonary Bypass Surgical approach is through median sternotomy. If there is only a single superior vena cava some surgeons pre

Sample titration for determination of iodine number of lipid, Define Sample...

Define Sample titration for Determination of the Iodine Number of Lipids? With a clean dry pipette, accurately measure 0.3 mL of oil into a clean and dry iodination flask (Dupl

What are the cytoplasmic structures present in animal cells, Q. What are th...

Q. What are the main cytoplasmic structures present in animal cells? The main cytoplasmic structures of the cell are the centrioles, the cytoskeleton, mitochondria, peroxisomes

Synthetic conduits, Synthetic Conduits Dacron, Polytetra fluro ethylen...

Synthetic Conduits Dacron, Polytetra fluro ethylene (PTFE) and polyglycolic acid grafts have extremely poor results and are not used any more.

Heart in fishes, Fishe s - Heart is 2 chambered 1 auricle & 1 ventr...

Fishe s - Heart is 2 chambered 1 auricle & 1 ventricle present. Sinus venosus, truncus or conus arteriusus present. Only impure blood come in heart so heart is venous he

explain alternative processing, Alternative polyadenylation sites Exa...

Alternative polyadenylation sites Exact pre-mRNAs hold more than single group of signal sequences for 3' end polyadenylation and cleavage.   In  some  cases,  the  area  of  t

Explain trichomoniasis, Trichomoniasis  Oral metronidazole has been the...

Trichomoniasis  Oral metronidazole has been the treatment of choice for trichomoniasis. Tinidazole (Tindamax - Medical Letter 2004; 46:70), a nitroimidazole similar to metronid

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd