Can you explain lane and eynon method, Biology

Assignment Help:

Q. Can you explain Lane and Eynon method?

Reducing sugars are those, which have free sugar groups (e.g. glucose, fructose etc.) and hence may be estimated directly by titrating the solution of the sample with Fehling's solution. Total sugars include both reducing and non-reducing sugars. Non-reducing sugars (e.g. maltose, lactose, sucrose etc.) do not contain free sugar groups and cannot reduce Fehling solution. Hence, non-reducing sugars must be hydrolyzed to monosaccharides by heating with acid before titration.

Reducing sugars are acted upon by the alkali of the Fehling solution to form enediols. These enediols are very unstable and reactive and they reduce Cu²+ ions to Cu+ ions.

These Cu+ ions combine with hydroxyl groups to form cuprous hydroxide, which on heating gives red precipitate of cuprous oxide. To get a sharp end point, methylene blue is added which is reduced to a colourless leuco compound restoring the red colour of the solution. Sodium potassium tartarate keeps the Cu²+ ions in the solution, thus ensuring a continuous supply of Cu²+ ions for reduction.


Related Discussions:- Can you explain lane and eynon method

Cardiac branches and cardiac plexuses, The vagus gives of one superior cerv...

The vagus gives of one superior cervical cardiac branch and an inferior cervical cardiac branch in the neck. These branches descend into the thorax and take part in performing the

Explain about gastric ulcers, Q. Explain about Gastric ulcers? Gastric ...

Q. Explain about Gastric ulcers? Gastric ulcers occur in the lesser curvature of the antrum of stomach. A gastric ulcer appears to be caused by reflux of bile and factors that

The eye refract the light, Which parts of the eye refract ('bend') the ligh...

Which parts of the eye refract ('bend') the light in such a way as to form an image on the retina?   The curved surface of the cornea, and the aqueous humour enclosed by it,

Antimicrobial barriers - proliferation of microorganisms, Q. Antimicrobial...

Q. Antimicrobial Barriers -  proliferation of microorganisms? The first barrier is the integument: a physical barrier to protect the food, e.g., the shell on eggs, the skin on

Relationship of different systems in diabetes mellitus, Describe the relati...

Describe the relationship of different systems in diabetes mellitus Diabetes Mellitus has direct relationship with endocrine system, especially with endocrine part of the panc

Explain vitamins requirement during thyphoid, Explain Vitamins requirement ...

Explain Vitamins requirement during thyphoid Vitamins : Vitamins which need to be emphasized include B complex, considering the increase  in  the energy requirement and a decr

Non-muscular movement, NON-MUSCULA R MOVEMENT - 1 .      Streaming m...

NON-MUSCULA R MOVEMENT - 1 .      Streaming movement - In amoeba, cyclosis is common. 2 .      Pseudopodial - In leucocyte, macrophages, amoeboid movement take place

Difference between alpha glucose and beta glucose, What is the difference b...

What is the difference between alpha glucose and beta glucose? Why are they different?

Under which form is nitrogen fixed by living beings, Under which form is ni...

Under which form is nitrogen fixed by living beings? Most living beings cannot use molecular nitrogen to get nitrogen atoms. Producers fix nitrogen mostly from nitrate (NO3-).

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd