Can you explain lane and eynon method, Biology

Assignment Help:

Q. Can you explain Lane and Eynon method?

Reducing sugars are those, which have free sugar groups (e.g. glucose, fructose etc.) and hence may be estimated directly by titrating the solution of the sample with Fehling's solution. Total sugars include both reducing and non-reducing sugars. Non-reducing sugars (e.g. maltose, lactose, sucrose etc.) do not contain free sugar groups and cannot reduce Fehling solution. Hence, non-reducing sugars must be hydrolyzed to monosaccharides by heating with acid before titration.

Reducing sugars are acted upon by the alkali of the Fehling solution to form enediols. These enediols are very unstable and reactive and they reduce Cu²+ ions to Cu+ ions.

These Cu+ ions combine with hydroxyl groups to form cuprous hydroxide, which on heating gives red precipitate of cuprous oxide. To get a sharp end point, methylene blue is added which is reduced to a colourless leuco compound restoring the red colour of the solution. Sodium potassium tartarate keeps the Cu²+ ions in the solution, thus ensuring a continuous supply of Cu²+ ions for reduction.


Related Discussions:- Can you explain lane and eynon method

What are the major negative ions found in living beings, Q. What are the ...

Q. What are the major negative ions found in living beings? The main anions found in living beings are the chlorine anion (Cl - ), the sulphate anion (SO - ), the nitrate ani

Sex, how to do sex ?

how to do sex ?

Explain about the lipoproteins, Explain about the Lipoproteins? These a...

Explain about the Lipoproteins? These are the Multicomponent complexes of lipids and proteins that form distinct molecular aggregates. They contain polar and neutral lipids, ch

How does the federal government regulate plant-incorporated, How does the f...

How does the federal government regulate plant-incorporated protectants and ensure their safe use in foods? EPA regulates the manufacture, sale and use of pesticides, containin

Explain about vitamin E, Vitamin E (DL-α-Tocopherol) DL-α-Tocopherol is...

Vitamin E (DL-α-Tocopherol) DL-α-Tocopherol is a yellow to red-brown,  clear, viscous oil almost without odour which decolourise when exposed to light and air. DL-α-Tocopherol

Determine the term memory - psychological tests, Determine the term Memory ...

Determine the term Memory - Psychological tests The acquisition, reaction, and retrieval of new information is a domain of much importance in infants and young children at risk

Steps to follow in diagnosis of dysentry, Assessment   The child with dy...

Assessment   The child with dysentery will present you with sudden onset of fever, prostration, vomiting, bloody diarrhoea, abdominal pain and tenesmus. Patient may suffer from

Bovine spongiform encephalopathy, Bovine spongiform encephalopathy The ...

Bovine spongiform encephalopathy The bovine transmissible spongiform encephalopathy (BSE), known as 'mad cow disease'-first noticed in Great Britain in 1986, is similar to scra

Explain the peripheral resistance, Explain the Peripheral Resistance Re...

Explain the Peripheral Resistance Resistance offered by arterioles or resistance vessels, as you have read above, is termed as peripheral resistance. Changes in peripheral resi

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd