Can you explain lane and eynon method, Biology

Assignment Help:

Q. Can you explain Lane and Eynon method?

Reducing sugars are those, which have free sugar groups (e.g. glucose, fructose etc.) and hence may be estimated directly by titrating the solution of the sample with Fehling's solution. Total sugars include both reducing and non-reducing sugars. Non-reducing sugars (e.g. maltose, lactose, sucrose etc.) do not contain free sugar groups and cannot reduce Fehling solution. Hence, non-reducing sugars must be hydrolyzed to monosaccharides by heating with acid before titration.

Reducing sugars are acted upon by the alkali of the Fehling solution to form enediols. These enediols are very unstable and reactive and they reduce Cu²+ ions to Cu+ ions.

These Cu+ ions combine with hydroxyl groups to form cuprous hydroxide, which on heating gives red precipitate of cuprous oxide. To get a sharp end point, methylene blue is added which is reduced to a colourless leuco compound restoring the red colour of the solution. Sodium potassium tartarate keeps the Cu²+ ions in the solution, thus ensuring a continuous supply of Cu²+ ions for reduction.


Related Discussions:- Can you explain lane and eynon method

Explain nutrient requirement and dietary management, Explain Nutrient Requi...

Explain Nutrient Requirement and Dietary Management? You must have understood by the discussion above that by the end of the flow phase, the patient usually is well hydrated an

Ecotone - nature and structure of community, Ecotone - Nature and Structure...

Ecotone - Nature and Structure of Community The zone of vegetation separating two different types of communities is called ecotone. It is also known as a transition zone. The

Define precautions for testing quality of water, Define Precautions for Tes...

Define Precautions for Testing Quality of Water? 1. Handle the sewage water with care as it may contain enteric pathogens. 2. Sewage and pond water should be shaked before i

Stress testing in women, Q.Explain about Stress Testing in Women? Data ...

Q.Explain about Stress Testing in Women? Data on mortality in men is about 2.5 times that of women. At younger ages, however, CAD in men exceeds that found in women by 5 to 1.

Determine the features of the nervous system of arthropods, What are the no...

What are the noteworthy features of the nervous system of arthropods? In arthropods the nervous system has more complicated sensory receptors with well-advanced cephalization.

Explain the barrier function in the cornea, Explain the barrier function in...

Explain the barrier function in the cornea. Barrier Function: It is the first line of barrier between the external environment and the corneal stroma. The superficial epi

Zoonoses disease-monkey b virus disease, Monkey B virus disease The di...

Monkey B virus disease The disease is caused by Herpesvirus simiae or B virus, a member of the herpes group of viruses. The infection occurs naturally among primates of the ge

Cost-benefit relationship regarding sewage treatment, Q. What is the cost-b...

Q. What is the cost-benefit relationship regarding sewage treatment as a strategy to fight water pollution? To treat sewage is greatly cheaper for society. The non-treated sewa

Cnidarian, what process are nematocysts formed by the cnidoblast?

what process are nematocysts formed by the cnidoblast?

What are the applications of nicotinic acid, What are the Applications of N...

What are the Applications of Nicotinic acid Nicotinic acid is mainly used in the vitamin fortification of flour, macaroni and noodle products. Independent of its vitamin effic

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd