Can you explain lane and eynon method, Biology

Assignment Help:

Q. Can you explain Lane and Eynon method?

Reducing sugars are those, which have free sugar groups (e.g. glucose, fructose etc.) and hence may be estimated directly by titrating the solution of the sample with Fehling's solution. Total sugars include both reducing and non-reducing sugars. Non-reducing sugars (e.g. maltose, lactose, sucrose etc.) do not contain free sugar groups and cannot reduce Fehling solution. Hence, non-reducing sugars must be hydrolyzed to monosaccharides by heating with acid before titration.

Reducing sugars are acted upon by the alkali of the Fehling solution to form enediols. These enediols are very unstable and reactive and they reduce Cu²+ ions to Cu+ ions.

These Cu+ ions combine with hydroxyl groups to form cuprous hydroxide, which on heating gives red precipitate of cuprous oxide. To get a sharp end point, methylene blue is added which is reduced to a colourless leuco compound restoring the red colour of the solution. Sodium potassium tartarate keeps the Cu²+ ions in the solution, thus ensuring a continuous supply of Cu²+ ions for reduction.


Related Discussions:- Can you explain lane and eynon method

Explain the conveyor (or band) dryers, Explain the Conveyor (or Band) Dryer...

Explain the Conveyor (or Band) Dryers? In the conveyor (or band) dryer, the product is distributed on a moving belt, typically of a perforated plate, that passes through a tunn

Explain the air sampling, Explain the Air Sampling? Microorganisms may ...

Explain the Air Sampling? Microorganisms may be present in air of processing facility and have an impact on the quality and safety of foods processed and handled there. To avoi

Developmental biology, pioneers of developmental biology and their contribu...

pioneers of developmental biology and their contribution

Explain the metabolic alterations during cancer, Explain the Metabolic Alte...

Explain the Metabolic Alterations during Cancer? As we have studied earlier, patients with advanced cancer experience profound anorexia, early satiety, changes in the structure

Regular soda are made with aluminium, If the cans of regular soda are made ...

If the cans of regular soda are made with aluminium than diet soda, then regular soda will sink. Write brief descriptions of how you could test this hypothesis.

Explain wound healing and formation of woven bone, Wound healing and format...

Wound healing and formation of woven bone Stage 1: Wound healing and formation of woven bone (callus) (2 to 6 weeks) The ability of the body to respond to the "trauma" indu

How do taenias classify according to the division of sexes, How do taenias ...

How do taenias classify according to the division of sexes? Taenias are monoecious (hermaphrodite), the similar individual has female and male reproductive organs and undergoes

Determine the categories of drug and nutrient relationship, Determine the C...

Determine the Categories of Drug and nutrient relationship? Drug and nutrient relationship can be categorized into two aspects: 1. Effect of nutrition on drugs: the influenc

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd