Can you explain lane and eynon method, Biology

Assignment Help:

Q. Can you explain Lane and Eynon method?

Reducing sugars are those, which have free sugar groups (e.g. glucose, fructose etc.) and hence may be estimated directly by titrating the solution of the sample with Fehling's solution. Total sugars include both reducing and non-reducing sugars. Non-reducing sugars (e.g. maltose, lactose, sucrose etc.) do not contain free sugar groups and cannot reduce Fehling solution. Hence, non-reducing sugars must be hydrolyzed to monosaccharides by heating with acid before titration.

Reducing sugars are acted upon by the alkali of the Fehling solution to form enediols. These enediols are very unstable and reactive and they reduce Cu²+ ions to Cu+ ions.

These Cu+ ions combine with hydroxyl groups to form cuprous hydroxide, which on heating gives red precipitate of cuprous oxide. To get a sharp end point, methylene blue is added which is reduced to a colourless leuco compound restoring the red colour of the solution. Sodium potassium tartarate keeps the Cu²+ ions in the solution, thus ensuring a continuous supply of Cu²+ ions for reduction.


Related Discussions:- Can you explain lane and eynon method

What are protein hydrolysates, What are protein hydrolysates? Proteins ...

What are protein hydrolysates? Proteins that have been treated with enzymes to break them down into amino acids or shorter peptides are referred to as protein hydrolysates.

Carbohydrates requirements for ulcerative colitis, Q. Carbohydrates require...

Q. Carbohydrates requirements for ulcerative colitis? Carbohydrates: They form the easily absorbable source of energy. Bulk-producing vegetables are restricted so as to allow b

Define the recommended dietary allowances of vitamin k, Define the Recommen...

Define the Recommended Dietary Allowances of Vitamin K? Recommended dietary intakes have not been suggested for different age groups or gentler. The safe levels of intake have

What is population growth rate, Q. What is population growth rate? The ...

Q. What is population growth rate? The Population growth rate (PGR) is the percent variation between the numbers of individuals in a population at two different times. Thus the

Illustrate adaptation to terrestrial life, Q. Why can the allantois be cons...

Q. Why can the allantois be considered an adaptation to terrestrial life? The allantois is an adaptation to dry land because in embryos of oviparous terrestrial beings like bir

Are gametes always made by meiosis, Q. Are gametes always made by meiosis? ...

Q. Are gametes always made by meiosis? In the haplontic haplobiontic life cycle and in the plant life cycle (diplobiontic life cycle) gametes are made by mitosis and not by mei

Quantification tricuspid regurgitation-echo quantification, Quantification ...

Quantification of Tricuspid Regurgitation Echocardiography and right ventricular angiogram can quantify tricuspid regurgitation. Echo Quantification Trivial: Non susta

Explain directly observed therapy of tuberculosis, Directly observed therap...

Directly observed therapy In treating TB, poor adherence to therapy is the most important cause of treatment failure and is associated with emergence of drug resistance. Medica

Spermatogonia, Spermatogonia Spermatogonia are the youngest germ cells...

Spermatogonia Spermatogonia are the youngest germ cells from which spermatozoa proliferate. These lie next to the basement membrane and undergo series of mitotic divisions lea

Dextrin, DEXTRIN Intermediate product of hydrolysis of starch. It co...

DEXTRIN Intermediate product of hydrolysis of starch. It consists of maltose & glucose. It is found in yeast & some bacteria.

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd