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Q. How are amino acids brought to the cellular site where translation takes place? What is an anticodon?
Amino acids are brought to ribosomes by RNA molecules known as transfer tRNA, or RNA. One tRNA associated to its specific amino acid binds by a special sequence of three nucleotides to an mRNA codon exposed in the ribosome. This sequence in the tRNA is known as anticodon the tRNA anticodon must be complementary to the mRNA codon to which it binds, according to the rule CG, A- U. The ribosome then slides along the mRNA molecule a process called translocation to expose the following codon to the binding of other tRNA. When amino acids corresponding to neighboring codons bind by peptide bond the first tRNa is liberated.
You have discovered a novel alternatively spliced gene (gene X) and you wish to study its regulation in an in vitro splicing assay. The alternatively spliced product retains intro
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