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Calculate the molar concentration of the solution essential to obtain the value of 10 molecules per µm2. (For this calculation we suppose that the transfer of the dye molecules from solution to the sample surface by evaporation of the solvent is homogeneous on the whole wetted area.)
The sample is illuminated with the 543.5 nm-line of a green He-Ne laser. The excitation power is adjusted so that the illuminated area (100 nm in diameter) is hit by 3 · 1010 photons per second.
Q. What is SI unit of energy? Ans. The SI unit of energy is called the joule. A joule is a pretty small mount of energy so often chemists will use the unit kJ =1 kilojoule
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Types of spectral interferences: While a quantitative analysis using a standard calibration curve is carried out, the calibration plot of emission intensity vs concentration o
Isoelectric point - physical characteristic of proteins Each protein has a characteristic isoelectric point for which its ionisation is minimum. Such as amino acids, proteins,
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