Calculate the amount of glycine in the mixture, Chemistry

Assignment Help:

Calculate the amount of glycine in the mixture:

Isotope dilution analysis was employed for the determination of glycine in 5.83 g protein hydrolysate mixture by using 14C labelled compound. 5.3 mg labeled compound with an activity of 39856 cpm was added to the mixture. The counter had background counts of 11790 counts in 90 min. After thorough mixing, the solution was passed through an ion-exchange column when 3.7 mg pure glycine was separated which showed an activity of 10900 counts in 50 min using the same counter. Calculate the % amount of glycine in the mixture.

Answer:

The counter had a background of 11790 counts in 90 min

or Background count rate =  131 cpm

Amount of radiolabeled tracer added, m*           =          5.3 mg

Specific Activity of the radiotracer added, SA1   = (39856 -131)/25.7

= 39725/25.7

=   1545.7 cpm/mg

Amount of pure fraction of glycine = 23.7 mg

Net Activity of glycine = 10900 counts in 50 min = 218 cpm - 131 cpm(background)

= 87 cpm

Specific Activity of separated fraction of glycine = 87 / 3.7 = 23.5 cpm/mg

Amount of glycine in mixture = 5.3 (1545.7/23.5 - 1) = 343.1 mg

Percent amount of glycine in the mixture = (0.343/5.83) ×100

= 5.89%


Related Discussions:- Calculate the amount of glycine in the mixture

Chemical Bonding, Structure of chlorine triflouride by VSEPR theory

Structure of chlorine triflouride by VSEPR theory

Phenol, what is trihydric phenol and hownit is works

what is trihydric phenol and hownit is works

What are the types of acceptance tests?, What are the Types of Acceptance T...

What are the Types of Acceptance Tests? There are two main aspects to acceptance testing: Measurement of Acceptability  Comparison of acceptability or preference

What is stoichiometry, Q. What is Stoichiometry? Ans. As you know ...

Q. What is Stoichiometry? Ans. As you know a chemical equation is like a recipe. Each of the different ingredients combine in certain ratios to form the end product. The

Copper, stuck on a chemistry assignment i need a way to include the elctron...

stuck on a chemistry assignment i need a way to include the elctron configuration and chemical properties of copper into a oral that is also a sales pitch.

Determine the equilibrium concentrations of tris buffer, A Tris buffer at p...

A Tris buffer at pH 6.0 (here "Tris" is a nickname use for Tris hydroxymethyl amino methane). For the given pK A table is Tris a compound you would select for a buffer at pH 6.0?

What is the stability order of li2, what is the stability order of Li2, H2,...

what is the stability order of Li2, H2, B2 B.O of Li2 is 1 B.O of H2 is 1 B.O of B2 is also 1 so for stability order u need to see the no of electrons in antibonding orbitals mo

Define product development, Q. Define product development? Product deve...

Q. Define product development? Product development is a future oriented practice and an effort to foresee the future needs of the market place and to translate this informatio

Homweoek help, Determine the rate constant from the concentration-time depe...

Determine the rate constant from the concentration-time dependence: Time (s) [Reactant](M) 0.0 0.0451 2.0 0.0421 5.0 0.0376 9.0 0.0316 15 0.0226 Use SI units, that is, seconds fo

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd