Calculate concentration of solution from standard curve, Biology

Assignment Help:

Calculate the Concentration of Unknown Solution from Standard Curve?

i) First take different volume of standard solution (say 0.2, 0.4, 0.6, 0.8, 1.0 ml) and then calculate the concentration of the standard solution added in each tube.

ii) Measure the optical density for each concentration in a spectrophotometer or colorimeter.

iii) Next, plot the concentration of the standards solution (taken on the x axis) and the corresponding OD (taken on the y axis) and the graph is prepared to give a standard curve which is a straight line (since it obeys Beer's law).

iv) Now take the unknown solution (say 1ml) and measure its OD reading. Suppose the value obtained is 0.40. Plot it on the y-axis of the standard curve (already prepared for the standard solution)

The concentration obtained is 275. This is the concentration of our unknown solution for the volume that we took (say1 ml).

 


Related Discussions:- Calculate concentration of solution from standard curve

What is the exchange list, What is the exchange list? IL is a grouping ...

What is the exchange list? IL is a grouping of foods in which specified amounts of all the foods provide approximately equal amount of (the same amount) carbohydrate, protein a

Disorders of upper respiratory tract, DISORDERS OF UPPER RESPIRATORY TRACT:...

DISORDERS OF UPPER RESPIRATORY TRACT: Common Cold: It is most frequent and most common infection in infants  and children. Common cold corresponds  to  acute nasopharyn

Describe aerobic respiration to the pyruvic acid molecules, Q. What happens...

Q. What happens during aerobic respiration to the pyruvic acid molecules made by glycolysis? and What is the sequence of reactions that then follows? The pyruvic acid molecules

Pyruvate carboxylase activation, Oxaloacetate has two main roles. It is an ...

Oxaloacetate has two main roles. It is an intermediate which is consumed in gluconeogenesis and it is also a key intermediate in the citric acid cycle where it fuses with acetyl Co

List the counselling skills, Q. List the counselling skills? Slowly sta...

Q. List the counselling skills? Slowly start winding up the contact with the client and the family by providing them support, motivating the client to take care of oneself with

Drug management for gout disease, Q. Drug Management for gout disease? ...

Q. Drug Management for gout disease? Drugs are so effective in lowering the serum mate concentration that rigid restriction of dietary purines is rarely necessary. Uricosurie d

What are the reproductive novelties, Q Do beings of the class Reptilia have...

Q Do beings of the class Reptilia have indirect or direct development? In beings of the class Reptilia the embryonic development is direct. So there is no larval stage. Q.

Difference between vegetative and generative cell , Difference between Vege...

Difference between Vegetative and Generative Cell The cytoplasm of the vegetative cell and the generative cell is distinctly different. The generative cell is transparent and

What is the cell theory, Q. What is the cell theory? Cell theory assert...

Q. What is the cell theory? Cell theory asserts that the cell is the element unit of living beings. Before the discovery of the cell, it was not acknowledged that living bei

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd