Calculate concentration of solution from standard curve, Biology

Assignment Help:

Calculate the Concentration of Unknown Solution from Standard Curve?

i) First take different volume of standard solution (say 0.2, 0.4, 0.6, 0.8, 1.0 ml) and then calculate the concentration of the standard solution added in each tube.

ii) Measure the optical density for each concentration in a spectrophotometer or colorimeter.

iii) Next, plot the concentration of the standards solution (taken on the x axis) and the corresponding OD (taken on the y axis) and the graph is prepared to give a standard curve which is a straight line (since it obeys Beer's law).

iv) Now take the unknown solution (say 1ml) and measure its OD reading. Suppose the value obtained is 0.40. Plot it on the y-axis of the standard curve (already prepared for the standard solution)

The concentration obtained is 275. This is the concentration of our unknown solution for the volume that we took (say1 ml).

 


Related Discussions:- Calculate concentration of solution from standard curve

Describe the planes of muscles, Describe the Planes of Muscles The plan...

Describe the Planes of Muscles The planes of superior and inferior recti in primary position form an angle of about 23 0 with the Y-axis. Therefore, the axis of rotation of th

Explain about stacking faults, Explain about Stacking faults Stacking ...

Explain about Stacking faults Stacking faults happen in a number of crystal structures, but the common example is in close-packed structures.  Face-centered cubic (fcc) struct

Determine the food source for phosphorus, Determine the Food Source for Pho...

Determine the Food Source for Phosphorus? Phosphorus is widely distributed in food. Food phosphorus is a mixture of both organic and inorganic forms although the relative amoun

What electrocardiogram results show a shortened qt interval, Electrocardiog...

Electrocardiogram results show a shortened QT interval and an increase in the P-R interval (i.e., decreased duration of action potential and decreased conduction velocity). Laborat

Major metabolic gases transported by the blood, Q. Which are the two major ...

Q. Which are the two major metabolic gases transported by the blood? The major metabolic gases transported by the blood are molecular carbon dioxide (CO 2 ) and oxygen (O 2 ).

Coronary revascularization, When underlying coronary artery disease is the ...

When underlying coronary artery disease is the cause of heart failure in the coronary revascularization may both improve symptoms and prevent  progression. Patients with angina and

Do enzymes act better under acid or basic ph, Do enzymes act better under a...

Do enzymes act better under acid or basic pH? Most enzymes act in pH among 6 and 8, a range that corresponds to the general acidic level of cells and blood. There are enzymes,

Define the parathyroid gland cells, Which of the following serves as a sens...

Which of the following serves as a sensor, or as part of a sensor, that functions in a negative feedback system? A. CaSRs (Calcium-Sensing Receptors) located in the plasma memb

Where are the adrenal glands located, Where are the adrenal glands located?...

Where are the adrenal glands located? How many are they and what are their portions? Each adrenal gland is located on the top of every kidney (forming a hat-like structure for

Chloroplast, Chloroplast In plants, chloroplasts are the essential o...

Chloroplast In plants, chloroplasts are the essential organelles for carrying out photosynthesis. Chloroplasts are found in all green plants. Chloroplast occurs mainly

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd