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Blotting is the technique for detecting one RNA within the mixture of RNAs (a Northern blot) or one type of the DNA within of a mixture of DNAs (a Southern blot). A blot can prove whether that one species of RNA or DNA is present, how much is there, and its estimated size. Mainly, blotting involves gel electrophoresis, transfer to the blotting membrane (typically nitrocellulose or activated nylon), and incubating with the radioactive probe. Exposing membrane to X-ray film creates darkening at the spot correlating with the position of the DNA or RNA of interest. The darker is the spot, the more nucleic acid was present there.
Explain the term Phytates? We are familiar with phytates as an inhibitor of mineral absorption (calcium, iron etc.) especially in the vegetarian diets that are cereal-based. Th
What are the main functions of (a) the basal (Malpighian) layer, (b) the cornified layer of the skin? (a) The basal (Malpighian) layer makes new skin cells and the pigment
Explain Back pain, arthritis and gout - Effect of Obesity? Abdominal obesity increases the risk of back pain because of the extra load on the spinal column. This, in turn, red
Indications i) When there is an abnormal rise in the bilirubin level which may be due to physiological or pathological jaundice or any other problem. ii) Prophylactic ph
Dendritic Cells a. Are the same as macrophages b. Bind and digest pathogen proteins c. Secrete immunoglobulin d. All of the above
Enumerate about the Reitan Aphasia Screening Test This test serves two purposes in that it contains both copying and language-related tasks. As an Aphasia screening procedure,
Describe about Congenital Cardiac Conditions Encountered in Adults ? The entire population of adults with CHD is constituted by patients whose cardiac malformations have a natu
Define the Indications for Root-End Resection Apicoectomy a) Persistent..... "Exactly the SAME of Curettage except the biopsy. b) When the apical portion of the root canal s
State two ways in which cardiac muscle is microscopically similar to skeleton muscle.
Define Classification of Carbohydrates? Carbohydrates are defined as polyhydroxy aldehydes or ketones or substances that produce such compounds when hydrolyzed. Carbohydrates a
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