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Biological fixation - Nitrogen Fixation
Approximately 63% of all nitrogen fixed is through biological fixation. Nitrogen fixing organisms are primarily prokaryotes; bacteria and blue green algae. Nitrogen fixation requires activation of molecular nitrogen by splitting nitrogen into two atoms of free nitrogen N2 → 2N.
This is an energy requiring step, which in biological fixation requires 160 kcal/mole. The actual fixation step, in which two atoms of nitrogen combine with three molecules of H2 to form two molecules of ammonia (NH3) releases 13 kcal/mole. Therefore, the net energy requirement for nitrogen fixation is 147 kcal/mole.
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