biochemistry, Science

Assignment Help:
Ask quTo optimise your kinase assay you require a range of substrate concentrations in the assay starting from 10 µM down to 10 nM substrate in 50 µL total assay volume (use 1:10 dilution steps). Calculate the concentrations required for the substrate stock solutions allowing addition of 10 µL to the kinase assay.
The substrate concentrations have to be how many times higher in the substrate stock solution than in the final kinase assay?
Select one:
a. 20x
b. 5x
c. 50x
d. 4x
e. 2x

Related Discussions:- biochemistry

Experiment of the buoyancy of water, Another way to observe the buoyancy of...

Another way to observe the buoyancy of water Push a large cork to the bottom of a pail of water. Notice the quantity of force you have to apply to hold the cork at the bottom.

What is the meaning of ''rock'' and ''mineral'', The meaning of 'rock' and ...

The meaning of 'rock' and 'mineral' Develop the meaning of these two terms by a study of the specimens collected. A rock is usually regarded as mineral matter found in t

Define systemic physiology, Define Systemic Physiology - physiology of grow...

Define Systemic Physiology - physiology of growth and development There is a change in the blood count and rate of haemoglobin (Hb) production during the early days. Cardiovasc

Nerve tissue, What is a feature of a nerve tissue?

What is a feature of a nerve tissue?

Analyse the human - ecosystem interactions, Question 1 Analyse the Huma...

Question 1 Analyse the Human - Ecosystem interactions Question 2 Discuss the importance of PPP in attaining sustainability Question 3 Explain the Benefits and c

Freezing by contact with a cooled liquid-lmmersion freezing, Freezing by Co...

Freezing by Contact with a Cooled Liquid: Immersion Freezing In this method, the food is immersed in low-temperature brine to achieve fast temperature reduction through direct

The temperature of water in oceanic depths, What is the general statement ...

What is the general statement regarding the temperature of water in oceanic depths? Commonly, the relation is this - the further from the surface the colder the water. This

Solar family, The Solar Family: Let us now take you on a journey acro...

The Solar Family: Let us now take you on a journey across the universe. We begin from our planet EQrth. It  is our home. The Earth  is active, lush and fertile. It is a place

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd