biochemistry, Science

Assignment Help:
Ask quTo optimise your kinase assay you require a range of substrate concentrations in the assay starting from 10 µM down to 10 nM substrate in 50 µL total assay volume (use 1:10 dilution steps). Calculate the concentrations required for the substrate stock solutions allowing addition of 10 µL to the kinase assay.
The substrate concentrations have to be how many times higher in the substrate stock solution than in the final kinase assay?
Select one:
a. 20x
b. 5x
c. 50x
d. 4x
e. 2x

Related Discussions:- biochemistry

Carbon in the carcass - radioactive, 1. A cyclist ascends a hill of height ...

1. A cyclist ascends a hill of height 120 meters. The mass of cyclist plus bicycle is 100 kilograms. His efficiency is 25%. How many Calories does he burn? 2. A hiker in the Swi

White lipped tree frog, how does reproduction help the white lipped tree fr...

how does reproduction help the white lipped tree frog survive

Define the both macronutrient and micronutrient, Define the both macronutri...

Define the both macronutrientand micronutrient. An element necessary in large amounts (usually > 50 mg/kg in the plant) for the growth of plant is called a macronutrient and in

What is competitive inhibition, Competitive inhibition This  type of in...

Competitive inhibition This  type of inhibition takes place when  a  compound having  a strong structural resemblance to the substrate competes with  it for the catalytic  site

What is parent material, What is Parent material Parent material refers...

What is Parent material Parent material refers to the primary material from which the soil is formed. The parent material may be either a solid rock, a decomposed rock, organic

Explain the local hormones, Explain the Local Hormones Local hormones a...

Explain the Local Hormones Local hormones affect cells in the vicinity of the organ secreting the hormone, what we call as paracrine influence. Examples of local hormones are g

Explain about the biosphere, Explain about the Biosphere Besides the ab...

Explain about the Biosphere Besides the above two types of factors, biosphere (the living environment) is another important active  soil forming agent.  The activity of living

What is an index, Question 1 What is an index? Explain physician's inde...

Question 1 What is an index? Explain physician's index with the help of examples 2 How to use the Electronic Medical Record for Prior authorization and SOAP technique 3 G

Why did the continents spread apart, Why did the continents spread apart? ...

Why did the continents spread apart? This is because of the heat generated from the earth's interior and earthquakes - the convection currents deep within the earth due to the

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd