biochemistry, Science

Assignment Help:
Ask quTo optimise your kinase assay you require a range of substrate concentrations in the assay starting from 10 µM down to 10 nM substrate in 50 µL total assay volume (use 1:10 dilution steps). Calculate the concentrations required for the substrate stock solutions allowing addition of 10 µL to the kinase assay.
The substrate concentrations have to be how many times higher in the substrate stock solution than in the final kinase assay?
Select one:
a. 20x
b. 5x
c. 50x
d. 4x
e. 2x

Related Discussions:- biochemistry

Flammable liquids-storage of chemicals, Flammable Liquids : Bulk supplies ...

Flammable Liquids : Bulk supplies of all flammable liquids should be kept in a solvent store well away from main buildings. The store should be securely locked and fire warning no

Neanderthals, Neanderthals: These were the first human like fossils fo...

Neanderthals: These were the first human like fossils found. The Neanderthals  existed  throughout western Europe and across into the near east and central Asia from about  10

Explain the ventricular fibrillation, Explain the Ventricular Fibrillation ...

Explain the Ventricular Fibrillation This is a fatal condition in which the heart beats like a bag of worms and the patient may die in a couple of hours, if the action is not t

Stellar motion:, Stellar Motion: Stars are not fixed  in  the heavens. ...

Stellar Motion: Stars are not fixed  in  the heavens. They are moving within  the galaxies. The speed of a star moving toward or away from the Earth is indicated by a shift of

Explain neutrophils -types of white blood cells, Explain Neutrophils Th...

Explain Neutrophils They form about 50-70% cells. They have fine dust like particles in the cytoplasm which stain purplish. Tissues, cells damaged by the invading microbes rele

Psychology, thesis writing with 40 pages, topic Mapping the criminal mind: ...

thesis writing with 40 pages, topic Mapping the criminal mind: Profiling and its limitation''.

How is harvest maturity identified, Q. How is harvest maturity identified? ...

Q. How is harvest maturity identified? Maturity indices can be determined on the basis of : Number of days from setting, Sight-colour, size and shape, Touch-texture, hardness o

Explain the horizons - oap, Explain the following horizons:   i...

Explain the following horizons:   i)   Oap   ii)   Btk i)  Organic horizon (O) with organic material that has undergone decomposition (a) and has been disturbed or

#titleChild Care Centers in Colorado, What are the ratios allowed for three...

What are the ratios allowed for three year olds and Child Care Centers in Colorado and what is the citation of the rules that addresses ratios?

Describe the hydrologic cycle, Question 1: a) Briefly explain the follo...

Question 1: a) Briefly explain the following terms: i) evaporation ii) condensation iii) transpiration b) Describe the hydrologic cycle. Question 2: a) Define an

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd