biochemistry, Science

Assignment Help:
Ask quTo optimise your kinase assay you require a range of substrate concentrations in the assay starting from 10 µM down to 10 nM substrate in 50 µL total assay volume (use 1:10 dilution steps). Calculate the concentrations required for the substrate stock solutions allowing addition of 10 µL to the kinase assay.
The substrate concentrations have to be how many times higher in the substrate stock solution than in the final kinase assay?
Select one:
a. 20x
b. 5x
c. 50x
d. 4x
e. 2x

Related Discussions:- biochemistry

Vitamins, Vitamins: You are, probably, familiar with the names of some ...

Vitamins: You are, probably, familiar with the names of some of the vitamins. They can be obtained from plant and animal foods. Vitamins are required in small amounts and their

Explain thiamin (vitamin b1), Thiamin (vitamin B1) Thiamin was  first  ...

Thiamin (vitamin B1) Thiamin was  first  isolated  in  1926 from rice polishing by  Jansen  and Donath.  Its empirical formula was established  in  1931  by  Windaus  and  co-w

Explain functions of gastric juice - digestive function, Explain Functions ...

Explain Functions of Gastric Juice - Digestive function As the gastric juice contains water, it further liquefies the food swallowed. The hydrochloric acid acidifies the stomac

Explain yawing reflex actions - respiratory pocess, Explain Yawing Reflex A...

Explain Yawing Reflex Actions - Respiratory Pocess Yawning:  It is a deep inspiration drawn through open mouth. Low oxygen tension in the blood may be the cause of yawning.

A field trip to study erosion, A field trip to study erosion Find some ...

A field trip to study erosion Find some place in the locality where running water has complete damage by cutting gulleys. Take the class to study the erosion. Why was it caused

Nuclear fusion, Nuclear Fusion: The Ultimate Source of Energy : An energ...

Nuclear Fusion: The Ultimate Source of Energy : An energy hungry world views with envy the glowing power of  the sun and the stars, which is based on a slightly different nuclea

Diagnosis-scope of first aid, Diagnosis: 1.  Diagnosis of the casualty ...

Diagnosis: 1.  Diagnosis of the casualty involves considering the history, symptoms and signs. 2.  History is the information as to how the accident or illness occurred.

Define hyposecretion of insulin, Define Hyposecretion of Insulin You ma...

Define Hyposecretion of Insulin You may already be aware that an insufficiency of insulin in the body leads to the development of diabetes mellitus. It is caused by the degener

Lab help, Which type of ripple marks would you expect to find on the floor ...

Which type of ripple marks would you expect to find on the floor of a standing body of waterion..

Properties of a beating heart - cardiovascular system, Properties of a Beat...

Properties of a Beating Heart Heart Let us study a few interesting properties of a beating heart which make it such a unique organ. 1. Automaticity 2. Rhythmicity 3. L

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd