biochemistry, Science

Assignment Help:
Ask quTo optimise your kinase assay you require a range of substrate concentrations in the assay starting from 10 µM down to 10 nM substrate in 50 µL total assay volume (use 1:10 dilution steps). Calculate the concentrations required for the substrate stock solutions allowing addition of 10 µL to the kinase assay.
The substrate concentrations have to be how many times higher in the substrate stock solution than in the final kinase assay?
Select one:
a. 20x
b. 5x
c. 50x
d. 4x
e. 2x

Related Discussions:- biochemistry

Explain tidal volume and inspiratory reserve volume, Explain Tidal Volume a...

Explain Tidal Volume and Inspiratory Reserve Volume Tidal Volume (TV):   It is the volume of air that is taken in or given out during quiet breathing. The volume 1s about 500

Heat loss in cylinderical pipe, Ask quesExplain that your intention is to i...

Ask quesExplain that your intention is to investigate a variety of combinations of pipe material, insulation material and size which you could potentially consider when deciding on

Earth, Earth How do we describe the 'big ball' on which we live? Seen f...

Earth How do we describe the 'big ball' on which we live? Seen from space, it appears as a bluish-white sphere. Its wealth of plant and animal life, snow covered peaks, blue oc

A field trip to study erosion, A field trip to study erosion Find some ...

A field trip to study erosion Find some place in the locality where running water has complete damage by cutting gulleys. Take the class to study the erosion. Why was it caused

Arthropoda, structure and function of respiratory organs in arthropoda with...

structure and function of respiratory organs in arthropoda with diagram

The ventricular system, The ventricular system While the ventricular syste...

The ventricular system While the ventricular system is naturally not involved in cognitive processing since it consists of cavities filled with cerebrospinal fluid, it is good to

Brain, Brain: The centre piece of  the nervous system is the brain wh...

Brain: The centre piece of  the nervous system is the brain which  IS possibly the most organised form of matter known. It  is unlike any other organ of  the body, as ~t alon

Six kingdom coloring worksheet, What protist changes shape constantly and f...

What protist changes shape constantly and flows around it''s food to engulf it

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd