biochemistry, Science

Assignment Help:
Ask quTo optimise your kinase assay you require a range of substrate concentrations in the assay starting from 10 µM down to 10 nM substrate in 50 µL total assay volume (use 1:10 dilution steps). Calculate the concentrations required for the substrate stock solutions allowing addition of 10 µL to the kinase assay.
The substrate concentrations have to be how many times higher in the substrate stock solution than in the final kinase assay?
Select one:
a. 20x
b. 5x
c. 50x
d. 4x
e. 2x

Related Discussions:- biochemistry

Separating mixture, laboratory apparatus for separating mixture

laboratory apparatus for separating mixture

Define age related changes in nervous system, Define age related changes in...

Define age related changes in Nervous system Varying degrees of atrophy in neuronal areas can be observed neurotransmitter functions are hampered. The cholinergic deficits seen

How different pressures of air force water from a container, How different ...

How different pressures of air force water from a container Fit a test tube with a two-hole stopper. By one hole place a length of glass tube which extends into the test tube a

Why does marble rarely contain fossils, Why does marble rarely contain foss...

Why does marble rarely contain fossils? This is due to of the way that it is formed. When you have a rock that is formed bymetamorphosis then it has been created by heat and pres

Where the water table intersects the surface, What is the term for groundwa...

What is the term for groundwater emerging naturally at the ground surface, where the water table intersects the surface: Spring is the term for groundwater emerging aturally

Oxidising substances , Oxidising Substances : It includes oxidising substa...

Oxidising Substances : It includes oxidising substances and the organic peroxides. Oxidising substances are by themselves not combustible but by feeding oxygen to other substances

First aid procedure for electric shock, FIRST AID PROCEDURE FOR ELECTRIC SH...

FIRST AID PROCEDURE FOR ELECTRIC SHOCK : Although the procedure described is usually followed, there are occasions when other matters have to be considered and therefore the proce

Explain functions of gastric juice - digestive function, Explain Functions ...

Explain Functions of Gastric Juice - Digestive function As the gastric juice contains water, it further liquefies the food swallowed. The hydrochloric acid acidifies the stomac

The effect of soap on surface tension, The effect of soap on surface tensio...

The effect of soap on surface tension Select a large plate and rinse it unless you are sure that it is very clean. Fill the plate with cold water and let it stand for a time on

Explain the simple cells - primary visual cortex, Explain the Simple Cells ...

Explain the Simple Cells - Primary Visual Cortex They are most sensitive to lines oriented in a particular angle to the vertical. Simple cells probably receive a convergence of

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd