biochemistry, Science

Assignment Help:
Ask quTo optimise your kinase assay you require a range of substrate concentrations in the assay starting from 10 µM down to 10 nM substrate in 50 µL total assay volume (use 1:10 dilution steps). Calculate the concentrations required for the substrate stock solutions allowing addition of 10 µL to the kinase assay.
The substrate concentrations have to be how many times higher in the substrate stock solution than in the final kinase assay?
Select one:
a. 20x
b. 5x
c. 50x
d. 4x
e. 2x

Related Discussions:- biochemistry

What is the earths core made of, What is the earths core made of? Ans) ...

What is the earths core made of? Ans) The outer earth core is liquid and the inner is solid. Both parts are of alloy of two metals - iron and nickel, permitting the rise of the

Personal protective devices, Personal Protective Devices : We shall now di...

Personal Protective Devices : We shall now discuss the details of protective devices required for safety in a laboratory. The aim of protective devices is to minimise the risk of

Heterotrophic nutrition, how many types of heterotrophic nutrition in plant...

how many types of heterotrophic nutrition in plants?..

How temperature of the earths layers is changed, How Temperature of the Ear...

How Temperature of the Earth's Layers is changed? How does the temperature of the Earth's interior change as one goes deeper within the Earth? As one might expect, the farther

Hydra, compare and contrast the hydra to one of the protozoan observed in c...

compare and contrast the hydra to one of the protozoan observed in class

Heaping water up in a glass, Heaping water up in a glass Place a drinki...

Heaping water up in a glass Place a drinking glass in a shallow pan or on a saucer. Rub the top edge of the glass with a dry cloth. Pour water into the glass unless it is full

What are the important processed pulse products, Q. What are the important ...

Q. What are the important processed pulse products? Puffed chickpea and pea, besan, papads, pulse-based weaning foods, quick cooking dhals, and canned dry peas are the importa

Define goitre - thyroid gland, Define Goitre - Thyroid Gland Goitre or ...

Define Goitre - Thyroid Gland Goitre or the enlargement of thyroid gland is one of the critical manifestations of iodine deficiency. Iodine, as you must have realized by now, i

Applications of optical fibres, Applications of Optical Fibres: Fibre...

Applications of Optical Fibres: Fibre optics finds many applications in areas like medicine and communications which we will briefly describe. We will also discuss  its advht

Obesity , Obesity : Generally, fat people think that their overweight is du...

Obesity : Generally, fat people think that their overweight is due to their constitution and for heredity. It could be true in some cases. But have you ever come across a fat labou

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd