biochemistry, Science

Assignment Help:
Ask quTo optimise your kinase assay you require a range of substrate concentrations in the assay starting from 10 µM down to 10 nM substrate in 50 µL total assay volume (use 1:10 dilution steps). Calculate the concentrations required for the substrate stock solutions allowing addition of 10 µL to the kinase assay.
The substrate concentrations have to be how many times higher in the substrate stock solution than in the final kinase assay?
Select one:
a. 20x
b. 5x
c. 50x
d. 4x
e. 2x

Related Discussions:- biochemistry

Flammable solids, Flammable Solids : Flammable solids are substances which...

Flammable Solids : Flammable solids are substances which are flammable or liable to spontaneous combustion or emit flammable gases on contact with water. Examples are: camphor, ci

Cell theory, Ask question #Minimumwhat is that definition for cell theory? ...

Ask question #Minimumwhat is that definition for cell theory? 100 words accepted#

Experiment of simple hydraulic press, Simple hydraulic press The princi...

Simple hydraulic press The principle of the hydraulic press is illustrated by the following model. Half fill a cylindrical jar with water. Pour melted paraffin wax on the su

Sterilizing water by boiling, Sterilizing water by boiling The presence...

Sterilizing water by boiling The presence of tiny living plants and animals makes water inappropriate for drinking. Such forms of life can only be seen by a microscope. We can

Broad area of science, Decribe the factors which decide the broad area of s...

Decribe the factors which decide the broad area of scientific activity

Explain types of steroids, Steroids are of many types . These are given be...

Steroids are of many types . These are given below: a)  Sterols :  Cholesterol, ergosterol. b)  Bile acids :  Glycocholic acid, taurocholic acid. c)  Hormones  :  Testoste

Determine if flow will choke and downstream and upstream, A nearly horizont...

A nearly horizontal,12 ft wide rectangular channel carries 60cfs at a depth of 3.0ft.The width of the channel is smoothly contracted to 6 ft at the downstream section. (a) Deter

Scientific approach to problem solving, Scientific approach to problem solv...

Scientific approach to problem solving: The scientific method and the features of scientific knowledge described above are in no way restricted  to the domain of scientists alo

Determine in detail about the phosphorus, Determine in detail about the Pho...

Determine in detail about the Phosphorus  Phosphorus, with nitrogen and potassium is classed as a major nutrient element. It is a constituent of nucleic acids, phytin, phosphol

How cells join together, In a unicellular organism, the interior  region of...

In a unicellular organism, the interior  region of  the organism  is no longer in contact with the external environment.  Thus simple processes  of  diffusion, phagocytosis, exocyt

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd