biochemistry, Science

Assignment Help:
Ask quTo optimise your kinase assay you require a range of substrate concentrations in the assay starting from 10 µM down to 10 nM substrate in 50 µL total assay volume (use 1:10 dilution steps). Calculate the concentrations required for the substrate stock solutions allowing addition of 10 µL to the kinase assay.
The substrate concentrations have to be how many times higher in the substrate stock solution than in the final kinase assay?
Select one:
a. 20x
b. 5x
c. 50x
d. 4x
e. 2x

Related Discussions:- biochemistry

Explain the digestion of food, Explain the Digestion of Food Salivary a...

Explain the Digestion of Food Salivary amylase or ptyalin acts on cooked starches (polysaccharides) and changes them into maltose as shown herewith. Boiled starch → soluble

Pollution, Pollution Now-a-days, you might have heard every one talking...

Pollution Now-a-days, you might have heard every one talking about pollution. What does pollution mean? Pollution is the addition to the environment (air, water, soil) of subst

Food, how foods are digested?

how foods are digested?

Quiz, I have a vocabulary quiz tommorrow! WHat am I supposed to do

I have a vocabulary quiz tommorrow! WHat am I supposed to do

Bile pigments and bile salts - abnormal constituent of urine, Bile pigments...

Bile pigments and bile salts - abnormal constituent of urine These occur in urine in conditions of hepatic and obstructive jaundice i.e. obstruction to the flow of bile into th

Friction, role of friction in snow..?

role of friction in snow..?

Define physiology, The  term physiology -physio meaning native and logos ...

The  term physiology -physio meaning native and logos meaning discourse, is a Greek  word, a synonym for natural philosophy. As the evolutionary processes progressed, the need

Discuss how mineral change in rocks, How do minerals become rocks and rocks...

How do minerals become rocks and rocks become soil? Ans) Minerals become rocks by pressure and temperature, often deep inside the earth's core, or easily through pressure for i

Semiconductors, Semiconductors: By now you have studied more than half ...

Semiconductors: By now you have studied more than half of  this course. You may have been to the study centre a few times. You might have taken lessons on audio  cassette-recor

Resistance, what are characteristics of resistance

what are characteristics of resistance

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd