biochemistry, Science

Assignment Help:
Ask quTo optimise your kinase assay you require a range of substrate concentrations in the assay starting from 10 µM down to 10 nM substrate in 50 µL total assay volume (use 1:10 dilution steps). Calculate the concentrations required for the substrate stock solutions allowing addition of 10 µL to the kinase assay.
The substrate concentrations have to be how many times higher in the substrate stock solution than in the final kinase assay?
Select one:
a. 20x
b. 5x
c. 50x
d. 4x
e. 2x

Related Discussions:- biochemistry

What are the functions of lymphocytes, What are the functions of lymphocyte...

What are the functions of lymphocytes? The lymphocytes handle most of the bacterial and viral infection that we get. In fact, lymphocytes are directly involved with specific ac

Explain teh term cryogenic freezing, Cryogenic Freezing, as you would reali...

Cryogenic Freezing, as you would realize, is one of the fast, flexible and cheap method of freezing. In this, the liquefied gases are placed in direct contact with the foods. Food

Classification of hazardous chemicals, Classification of Hazardous Chemical...

Classification of Hazardous Chemicals : The classification of chemical hazards as recommended by the UN Committee of Experts on the Transport of Dangerous goods has been widely ad

Weighing moisture again, Weighing moisture again The similar experiment...

Weighing moisture again The similar experiment can be done with a bath towel. Wet the towel and wring it out. Hang it on a coat hanger. Hang the coat hanger on single end of a

Objectives of electricity and water in a laboratory, Objectives After c...

Objectives After completing this exercise you should be able to: 1. Locate the source and control points of gas/electricity/water supply in the lab you have visited, 2. D

Requirements for disposal of microbial cultures, Requirements Autoclave...

Requirements Autoclave Microbial cultures - liquid/solid Disinfectant Spatula Disposable bags

Ayurvedic system of medicine, The Ayurvedic System of Medicine:  Puna...

The Ayurvedic System of Medicine:  Punarvasu Atreya (about 6th century B.C.) taught medicine at Taxila. Each of his disciples such as Bhela, Jatukarna, Harita, Ksarapani, Par

Life of a star, Life of a Star: A young star is thought  to be composed...

Life of a Star: A young star is thought  to be composed largely of  hydrogen gas. Hence, the most likely place for a star to be born is in one of  the numerous clouds of hydrog

Safety spectacles in personal protective devices, Safety spectacles : Safe...

Safety spectacles : Safety spectacles need to be used where there is the slightest risk of splashes of chemicals or fragments of dust, glass, etc. getting into the eyes.

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd