biochemistry, Science

Assignment Help:
Ask quTo optimise your kinase assay you require a range of substrate concentrations in the assay starting from 10 µM down to 10 nM substrate in 50 µL total assay volume (use 1:10 dilution steps). Calculate the concentrations required for the substrate stock solutions allowing addition of 10 µL to the kinase assay.
The substrate concentrations have to be how many times higher in the substrate stock solution than in the final kinase assay?
Select one:
a. 20x
b. 5x
c. 50x
d. 4x
e. 2x

Related Discussions:- biochemistry

Concentrations of solutions, if 120g of a 6% sucrose solution are needed, w...

if 120g of a 6% sucrose solution are needed, what mass of sucrose is required to prepare the solution?

Waves, Compare and contrast the characteristics of sound waves and electrom...

Compare and contrast the characteristics of sound waves and electromagnetic

Science, #qpecifically, they want to find two separate locations: one where...

#qpecifically, they want to find two separate locations: one where shallow, large magnitude earthquakes are frequently found, and one where large steep volcanoes that have explosiv

Dry cell, how to make assignment of dry cell?

how to make assignment of dry cell?

ADVANCEMENT , how communication can bring about advancement in any society....

how communication can bring about advancement in any society..

Hepar, what is a hepar

what is a hepar

Explain the mucous membrane, Explain the Mucous Membrane This layer is ...

Explain the Mucous Membrane This layer is lined by the epithelial cells. Parts of the tract which are subject to mechanical injury, the layer consists of stratified squamous ep

Explain two types of transferases, Explain two types of transferases ? ...

Explain two types of transferases ? a) Alanine aminotransferase Alanine + a-ketoglutarate ↔ glutamate + pyruvate b) Aspartate aminotransferase (AST) Asparate + a-ketogl

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd