biochemistry, Science

Assignment Help:
Ask quTo optimise your kinase assay you require a range of substrate concentrations in the assay starting from 10 µM down to 10 nM substrate in 50 µL total assay volume (use 1:10 dilution steps). Calculate the concentrations required for the substrate stock solutions allowing addition of 10 µL to the kinase assay.
The substrate concentrations have to be how many times higher in the substrate stock solution than in the final kinase assay?
Select one:
a. 20x
b. 5x
c. 50x
d. 4x
e. 2x

Related Discussions:- biochemistry

Connective tissue, What is the function of the connective tissue?

What is the function of the connective tissue?

English, Unit 1 : Due this friday. 11/09 ........ 150 word.. no more th...

Unit 1 : Due this friday. 11/09 ........ 150 word.. no more than 190 word Discussion topic #1: Drawing upon two major conclusions from the author, explain why the artic

The arctic circle lies at which latitudes, The Arctic Circle lies at which ...

The Arctic Circle lies at which latitudes: At (66 ½oSouth)

Physiology, Monosaccharides and amino acids are absorbed into the blood in ...

Monosaccharides and amino acids are absorbed into the blood in the intestinal capillaries. Is it true or False?

Hopkins reading test, To provide an estimate of Mr. X's premorbid level of ...

To provide an estimate of Mr. X's premorbid level of intellectual functioning, the Hopkins Adult Reading Test was administered. He received a raw score of XX (standard score of 119

Explain the circumvallate papillae, Explain the Circumvallate papillae: ...

Explain the Circumvallate papillae: These are the largest of the papillae, about 8 to 12 in number. These are arranged in a V-shape.

Describe the hydrologic cycle, Question 1: a) Briefly explain the follo...

Question 1: a) Briefly explain the following terms: i) evaporation ii) condensation iii) transpiration b) Describe the hydrologic cycle. Question 2: a) Define an

How to prevent the erosion of topsoil, How to prevent the erosion of topsoi...

How to prevent the erosion of topsoil Use the trays constructed for the experience above. (a)  Fill the trays with loose soil and tilt everyone the similar amount. Make furr

Foam extinguishers, Foam extinguishers :  If the burning liquid is in a co...

Foam extinguishers :  If the burning liquid is in a container the jet should be directed at the inside edge of the vessel or at a vertical surface in order to break the jet and al

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd