biochemistry, Science

Assignment Help:
Ask quTo optimise your kinase assay you require a range of substrate concentrations in the assay starting from 10 µM down to 10 nM substrate in 50 µL total assay volume (use 1:10 dilution steps). Calculate the concentrations required for the substrate stock solutions allowing addition of 10 µL to the kinase assay.
The substrate concentrations have to be how many times higher in the substrate stock solution than in the final kinase assay?
Select one:
a. 20x
b. 5x
c. 50x
d. 4x
e. 2x

Related Discussions:- biochemistry

Driving a boat by surface tension, Driving a boat by surface tension Se...

Driving a boat by surface tension Secure some gum camphor at the drug shop. Cut two or three boats from stiff paper, every about 2.5 cm in length. Cut a notch in the stern larg

PURIFICATION OF WATER, CAN YOU EXPLAIN THE FILTRATION METHOD OF PURIFICATIO...

CAN YOU EXPLAIN THE FILTRATION METHOD OF PURIFICATION OF WATER

Gloves for personal protective devices, Gloves : Gloves should be worn whe...

Gloves : Gloves should be worn when transferring toxic, radioactive and carcinogenic compounds, irritants and corrosive liquids. The practice of wearing rubber gloves continuously

Coping stress, Ask question #Minimum 100 words acceptedYou are caring for a...

Ask question #Minimum 100 words acceptedYou are caring for a 29-year-old woman who is married with two small children, ages 1 and 3 years, at home. She is recuperating from a left

Why do we require vitamins?, To perform the specific biological func. for t...

To perform the specific biological func. for the normal maintenance of optium growthand health.

Experiment of floating bodies, Floating bodies Fill the overflow can wi...

Floating bodies Fill the overflow can with water and let it run out unless the surface is level with the spout. Select a piece of wood that floats half or more submerged in the

What is a chemical formula, What is a chemical formula? A chemical form...

What is a chemical formula? A chemical formula is a way of representing molecules without drawing them. The chemical formula shows the ratio of atoms that form any given molecu

Define oxytocin hormone - posterior pituitary, Define Oxytocin Hormone - Po...

Define Oxytocin Hormone - Posterior Pituitary Oxytocin is a polypeptide containing 9 amino acids. An 'oxytoxic' agent is a substance that causes the gravid uterus (uterus of a

Experiment of a balance barometer, A balance barometer This device depe...

A balance barometer This device depends on the fact that dry air is heavier than moist air at the similar temperature. Two equal cylinders (tin cans would do) are mounted, o

How to calculate the student t test, How to calculate the student t test st...

How to calculate the student t test step by step when the mean of heart rate at 1 minute is 5.0,17.2,22.9,8.3,10,76.5,70,19.7 and for breathing rate it is 22.2,28.5,20,12.5,20,92,

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd