biochemistry, Science

Assignment Help:
Ask quTo optimise your kinase assay you require a range of substrate concentrations in the assay starting from 10 µM down to 10 nM substrate in 50 µL total assay volume (use 1:10 dilution steps). Calculate the concentrations required for the substrate stock solutions allowing addition of 10 µL to the kinase assay.
The substrate concentrations have to be how many times higher in the substrate stock solution than in the final kinase assay?
Select one:
a. 20x
b. 5x
c. 50x
d. 4x
e. 2x

Related Discussions:- biochemistry

Explain functions of the pancreas - neutralising action, Explain Functions ...

Explain Functions of the Pancreas - Neutralising action Neutralising action: Pancreatic juice is alkaline in nature and acid chyme is rendered alkaline by the strong alkalinity

Why do dark colours absorb heat?, Question 1 Outline the risks of nuclear ...

Question 1 Outline the risks of nuclear fissions Question 2 Comment on semiconductor devices and their usage Question 3 In a total system approach, what are the various

Origin and chemistry of life spontaneous generation, 1.     Describe the pr...

1.     Describe the properties of water that make if invaluable to life as we know it. 2.     Explain why the versatility of carbon is essential to life. 3.     Describe the

Determine about the physico-chemical parameters, Determine about the Physic...

Determine about the Physico-chemical parameters The possible combination of various elements found in the earth's crust gives rise to a large number of minerals. Out of all the

Image caching, how to cache images in java or jsp without using .htaccess??...

how to cache images in java or jsp without using .htaccess??

Determine the bravais lattice of the element, An x-ray diffractometer recor...

An x-ray diffractometer recorder chart for an element that has either the BCC or the FCC crystal structure shows diffraction peaks at the following 2θ angles: 40 0 , 58 0 , 73 0 ,

Satellite, Satellite : Satellite transmission is one important symbol of...

Satellite : Satellite transmission is one important symbol of revolution in communication technology. Besides transmitting picture and sound over long distances, it has revoluti

Disposal of waste materials, DISPOSAL OF WASTE MATERIALS : In a school or ...

DISPOSAL OF WASTE MATERIALS : In a school or college science laboratory, chemical wastes, broken glassware, unserviceable non consumable items, obsolete instruments, used biologic

Useful instruction for stock verification and maintenance , Useful Instruct...

Useful Instruction : You should go through of LT-1 course before doing this exercise. This exercise is a mix of demonstration and hands on activities. Take the help of your counse

Historic mineral that was the original source of radium, Give the common na...

Give the common name of the historic mineral that was the original source of radium and the first substance in which helium was discovered on earth: Pitchblende

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd