biochemistry, Science

Assignment Help:
Ask quTo optimise your kinase assay you require a range of substrate concentrations in the assay starting from 10 µM down to 10 nM substrate in 50 µL total assay volume (use 1:10 dilution steps). Calculate the concentrations required for the substrate stock solutions allowing addition of 10 µL to the kinase assay.
The substrate concentrations have to be how many times higher in the substrate stock solution than in the final kinase assay?
Select one:
a. 20x
b. 5x
c. 50x
d. 4x
e. 2x

Related Discussions:- biochemistry

Define the lymphocytes, Define the Lymphocytes They are agranulocytes. ...

Define the Lymphocytes They are agranulocytes. The nucleus occupies approximately the entire cell. They vary in size from 6- 18 mm diameter; sometimes divide in small and large

Explain oxygen dissociation curve, Explain Oxygen Dissociation Curve Wh...

Explain Oxygen Dissociation Curve While different partial pressures of oxygen are  plotted against the amount of  oxyhaemoglobin created or  dissociated, the sigmoid  curve  is

Environment and waste minimisation, Environment and Waste Minimisation   ...

Environment and Waste Minimisation   Your first assessment will cover the design of a monolith reactor for the VOCs removal. Therefore, you are asked to do the following:

Determine if flow will choke and downstream and upstream, A nearly horizont...

A nearly horizontal,12 ft wide rectangular channel carries 60cfs at a depth of 3.0ft.The width of the channel is smoothly contracted to 6 ft at the downstream section. (a) Deter

Water-mill in golden era, Water-mill in Golden era: The water-mills wer...

Water-mill in Golden era: The water-mills were also invented in  the classical period. But they came to be widely used only in  the Middle Ages. The wind-mills and water-mills

Heideggerian hermeneutic phenomenological approach, Data analysis: The...

Data analysis: The interview data was interpreted using a Heideggerian hermeneutic phenomenological approach. The researchers became intimately  immersed with the data duri

Air pollution, Air Pollution Air pollution  is one of the major probl...

Air Pollution Air pollution  is one of the major problems created by modern man.  It  is estimated that about 100 million tons of waste are poured  into our atmosphere each y

Six forms of prejudice, Name the six forms of prejudice that Brislin discus...

Name the six forms of prejudice that Brislin discusses and illustrate each with a South Africa example; 1.  Red-neck racism - a typical form of this prejudice is Julius Malema

Systems view of life, Systems view of life: Evolution has given rise to...

Systems view of life: Evolution has given rise to various types of  life forms from single celled bacteria to organisms as complex as a human being. It may interest you to know

Importance of nutrition, Define  Nutrition  Nutrition  is a science th...

Define  Nutrition  Nutrition  is a science that cuts across several scientific disciplines, of which one  is 'biochemistry'.  It  is  the  science  that  relalks  food  to the

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd