biochemistry, Science

Assignment Help:
Ask quTo optimise your kinase assay you require a range of substrate concentrations in the assay starting from 10 µM down to 10 nM substrate in 50 µL total assay volume (use 1:10 dilution steps). Calculate the concentrations required for the substrate stock solutions allowing addition of 10 µL to the kinase assay.
The substrate concentrations have to be how many times higher in the substrate stock solution than in the final kinase assay?
Select one:
a. 20x
b. 5x
c. 50x
d. 4x
e. 2x

Related Discussions:- biochemistry

Define psychology, QUESTION (A) Define Psychology (B) Why is psycholo...

QUESTION (A) Define Psychology (B) Why is psychology considered a science? (C) (i) Clinical and Counselling psychology sound similar. How do they differ? (ii) Discuss a

Respiration, Respiration:  Food +  oxygen ->  carbon dioxide + water + ...

Respiration:  Food +  oxygen ->  carbon dioxide + water +  energy  Food is not only a source of energy, but also the nutrients used to build up the organisms' bodies. So we

Explain the intercostal muscles, Explain the Intercostal Muscles Look a...

Explain the Intercostal Muscles Look at Figure Can you spot the intercostal muscles? Yes, there are 11 pairs of intercostal muscles. The intercoastal muscles are the muscles

Explain the hyposecretion or hypothyroidism in adult life, Explain the Hypo...

Explain the Hyposecretion or Hypothyroidism in Adult Life Decreased secretion of thyroid hormone results in the development of myxoedema. There is a slowing of mental and physi

Soundwaves.., Temperature affects the _____ of sound waves !?

Temperature affects the _____ of sound waves !?

Explain the posterior pituitary, Explain the Posterior Pituitary The po...

Explain the Posterior Pituitary The posterior pituitary gland or neurohypophysis is located immediately behind the anterior pituitary gland, as you may have seen in Figure.

Hydrophobicity of amino acids, Hydrophobicity of Amino Acids Hydrophobi...

Hydrophobicity of Amino Acids Hydrophobicity of amino acids and also of peptides and proteins may be determined by  relative  solubility of amino acid  in water and in  a less

Advantages of water in chemical weathering, Advantages of water in chemical...

Advantages of water in chemical weathering Water is an essential factor of chemical weathering  and when it contains dissolved salts and acids it is all the more effective. It

Friction, Where friction is desirable and undesirable at the same time.

Where friction is desirable and undesirable at the same time.

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd