biochemistry, Science

Assignment Help:
Ask quTo optimise your kinase assay you require a range of substrate concentrations in the assay starting from 10 µM down to 10 nM substrate in 50 µL total assay volume (use 1:10 dilution steps). Calculate the concentrations required for the substrate stock solutions allowing addition of 10 µL to the kinase assay.
The substrate concentrations have to be how many times higher in the substrate stock solution than in the final kinase assay?
Select one:
a. 20x
b. 5x
c. 50x
d. 4x
e. 2x

Related Discussions:- biochemistry

Explain sneezing reflex actions - respiratory process, Explain Sneezing Ref...

Explain Sneezing Reflex Actions - Respiratory Process This reflex is an involuntary, sudden, violent and audible expulsion of air through the mouth and nose. Usually irritation

Six kingdom coloring worksheet, What protist changes shape constantly and f...

What protist changes shape constantly and flows around it''s food to engulf it

Nutrition in plants, what are the five nutrition in heterotrophic nutrition...

what are the five nutrition in heterotrophic nutrition

Why there is more calcium than potassium on clay colloid, Why is there more...

Why is there more exchangeable calcium than potassium on clay/humus colloid? Calcium is usually abundant except in acid soils which occur in humid area due to excessive leaching.

Explain control of our heart through nerves, Explain Control of Our Heart t...

Explain Control of Our Heart through Nerves When we exercise in the mornings, the heart beats faster and when we sleep at night, it slows down. Have you ever wondered how does

Define procedure for estimation of iron in the solution, Define Procedure f...

Define Procedure for Estimation of Iron in the Solution? Carry out the experiment following the steps enumerated herewith: 1. Weigh 702 mg of reagent grade ferrous ammonium

Describe about the moles, Describe about the Moles Molehills,  frequent...

Describe about the Moles Molehills,  frequently observed in some fields are composed of subsoil deposited by moles. They need a good reserve of calcium in soil.

Media and scientific outlook in culture, Media and Scientific Outlook in Cu...

Media and Scientific Outlook in Culture: Apart from sharing of deeper values through history and continuous interaction,  the contribution made by  all sections of the people i

What is a floodplain, What is a floodplain? If a river tends to flood,...

What is a floodplain? If a river tends to flood, the most practical solution might be to designate a more or less flat area along the river as the "floodplain". This Area is p

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd