biochemistry, Science

Assignment Help:
Ask quTo optimise your kinase assay you require a range of substrate concentrations in the assay starting from 10 µM down to 10 nM substrate in 50 µL total assay volume (use 1:10 dilution steps). Calculate the concentrations required for the substrate stock solutions allowing addition of 10 µL to the kinase assay.
The substrate concentrations have to be how many times higher in the substrate stock solution than in the final kinase assay?
Select one:
a. 20x
b. 5x
c. 50x
d. 4x
e. 2x

Related Discussions:- biochemistry

Explain the diaphragm, Explain the Diaphragm The diaphragm is a muscula...

Explain the Diaphragm The diaphragm is a muscular membranous partition separating the abdominal and thoracic cavities and functioning in respiration. It consists of a central

Diastrophism, what is diastrophism and the theories of diastrophism?

what is diastrophism and the theories of diastrophism?

Minerals, Minerals  Minerals like coal, iron, copper, steel etc. are us...

Minerals  Minerals like coal, iron, copper, steel etc. are used in all kinds of industries and in every-day life. The rate of consumption of minerals  is increasing every year.

Storage of chemicals, Storage of Chemicals : You have read about the store...

Storage of Chemicals : You have read about the stores as important structures of all the labs in previously. Storage of chemicals whether in small amount or in bulk is another imp

Chemical hazards, CHEMICAL HAZARDS : Chemicals are present in all laborato...

CHEMICAL HAZARDS : Chemicals are present in all laboratories. It is good to consider all chemicals toxic and flammable unless one has definite information regarding its nature. Ou

To show that soil contains air, To show that soil contains air:- Place...

To show that soil contains air:- Place some soil in a glass jar or bottle and slowly pour water over it. Observe the air bubbles that increase by the water from the soil.

Explain the glomerular filtration rate (gfr), Explain the Glomerular Filtra...

Explain the Glomerular Filtration Rate (GFR) The kidneys receive a large amount of blood flow. The kidney blood flow is approximately 1200 ml per minute. One-tenth of this is f

Control of environment : water, Water: We all know  that safe drinking ...

Water: We all know  that safe drinking water is essential for good health. Many of  the diseases in our country are  due to lack of supply of safe drinking water. ~nfortunatel~

Photosynthesis, Photosynthesis: Carbon dioxide + water +  solar energy ...

Photosynthesis: Carbon dioxide + water +  solar energy + minerals -> food +  oxygen Food made during photosynthesis may later be used for energy by the plant itself, or by a

Explain the autoregulation function of kidneys, Explain the Autoregulation ...

Explain the Autoregulation function of kidneys The kidneys possess the property of autoregulation. This means that the blood flow in the kidneys remain constant. It is independ

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd