Basic principles of spectrophotometry, Physics

Assignment Help:

Basic principles of spectrophotometry:

An absorbance spectrophotometer is an instrument that measures the fraction of the incident light transmitted through a solution.  In other words, it is used to measure the amount of light that passes through a sample material and, by comparison to the initial intensity of light reaching the sample, they indirectly measure the amount of light absorbed by that sample.

Spectrophotometers are designed to transmit light of narrow wavelength ranges (see Figure 1 the electromagnetic spectrum).  A given compound will not absorb all wavelengths equally-that's why things are different colors (some compounds absorb only wavelengths outside of the visible light spectrum, and that's why there are colorless solutions like water).  Because different compounds absorb light at different wavelengths, a spectrophotometer can be used to distinguish compounds by analyzing the pattern of wavelengths absorbed by a given sample.  Additionally, the amount of light absorbed is directly proportional to the concentration of absorbing compounds in that sample, so a spectrophotometer can also be used to determine concentrations of compounds in solution. Finally, because particles in suspension will scatter light (thus preventing it from reaching the light detector), spectrophotometers may also be used to estimate the number of cells in suspension.

We will be using a spectrophotometer several times this semester to quantify the concentration of chemicals present in a solution.

 

922_Basic principles of spectrophotometry.png

 


Related Discussions:- Basic principles of spectrophotometry

Benefits of fiber-optic sensors for harsh environments, What are benefits o...

What are benefits of fiber-optic sensors for harsh environments? Benefits of fiber-optic sensors for harsh environments are illustrates below: a. Small, contact measurements

Explain the formation of the final image of an object, With the help of a r...

With the help of a ray diagram, explain the formation of the final image of an object in a compound microscope. Derive a mathematical expression for its magnifying power.

Calculate the direction of the ball move - collision, A 77.0-kg woman is wa...

A 77.0-kg woman is walking 0.10 m/s east in the gym. A man throws a 15.0-kg ball south and accidentally hits the woman. The woman and the ball move together with a velocity

Semiconductors, I want a clear explanation body full wave rectifier.

I want a clear explanation body full wave rectifier.

Difference between fission and fusion, Difference between Fission and Fusio...

Difference between Fission and Fusion Fission                                                                                         Fusion 1. Heavy metal is divided int

Evaluate the damping force, The natural angular frequency of a S.H.O. of ma...

The natural angular frequency of a S.H.O. of mass 2gm is 0.8rad/sec. It undergoes critically damped motion when taken to a viscous medium. Evaluate the damping force on the oscilla

What is meant by the rest mass energy of an electron, What is meant by the ...

What is meant by the rest mass energy of an electron? According to the Einstein's Theory of Relativity, the mass of a body (say a particle) occurs on the energy and on the mom

Concept of Speed, KINEMATICS I 1.   x(t) is called displacement and i...

KINEMATICS I 1.   x(t) is called displacement and it denotes the position of the body at a particular time. If the displacement is positive then that body is to the right of

Two-dimensional physics problem, An object is thrown at 25 m/s at 39 degree...

An object is thrown at 25 m/s at 39 degrees above the horizontal from a 40m tall cliff. How long is it in the air?

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd