Basic principles of spectrophotometry, Physics

Assignment Help:

Basic principles of spectrophotometry:

An absorbance spectrophotometer is an instrument that measures the fraction of the incident light transmitted through a solution.  In other words, it is used to measure the amount of light that passes through a sample material and, by comparison to the initial intensity of light reaching the sample, they indirectly measure the amount of light absorbed by that sample.

Spectrophotometers are designed to transmit light of narrow wavelength ranges (see Figure 1 the electromagnetic spectrum).  A given compound will not absorb all wavelengths equally-that's why things are different colors (some compounds absorb only wavelengths outside of the visible light spectrum, and that's why there are colorless solutions like water).  Because different compounds absorb light at different wavelengths, a spectrophotometer can be used to distinguish compounds by analyzing the pattern of wavelengths absorbed by a given sample.  Additionally, the amount of light absorbed is directly proportional to the concentration of absorbing compounds in that sample, so a spectrophotometer can also be used to determine concentrations of compounds in solution. Finally, because particles in suspension will scatter light (thus preventing it from reaching the light detector), spectrophotometers may also be used to estimate the number of cells in suspension.

We will be using a spectrophotometer several times this semester to quantify the concentration of chemicals present in a solution.

 

922_Basic principles of spectrophotometry.png

 


Related Discussions:- Basic principles of spectrophotometry

What do you mean by thermoelectric refrigerators, What is the specific resi...

What is the specific resistance of material of a conductor. What do you mean by thermoelectric refrigerators?

Dielectric, how dielectric mat is used for high voltage safety ? how it wor...

how dielectric mat is used for high voltage safety ? how it works?

Interference, if in an interference pattern,the ratio between maximum and m...

if in an interference pattern,the ratio between maximum and minimum intensities is 36:1,find the ratio between the amplitude and intensities of the two interfering waves.

Experiment of a test tube holder, A test tube holder An appropriate tes...

A test tube holder An appropriate test tube holder can be made by bending strong spring wire made of iron or brass into the shape shown in the diagram. Wire from a coat hanger

Name the different methods of charging a battery, Different methods of char...

Different methods of charging a battery:  There are two general methods are employed for charging are:- A) constant current system and B) constant voltage system..

Magnetic field is zero, Two parallel wires P and Q situated at a separation...

Two parallel wires P and Q situated at a separation of 6 cm carry electric currents i 1 =5 A and i2 = 2 A in opposite directions as given in fig-. Determine the point on the line

Writing, what is cruisiing mean

what is cruisiing mean

When were foundations of quantum mechanics established, When were foundatio...

When were foundations of quantum mechanics established? Foundations of quantum mechanics : The quantum mechanics foundations were established throughout the first half of th

What is the magnitude of the horizontal force, A man pushes a couch a dista...

A man pushes a couch a distance of 0.75 m. If 113 J of work is done. what is the magnitude of the horizontal force applied?

Define the tea in thermos, Define the Tea in Thermos? One liter of tea...

Define the Tea in Thermos? One liter of tea at 90° C is poured keen on a vacuum-insulated container (thermos). The surface area of the thermos walls A=600 cm 2 . The volume am

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd