Basic principles of spectrophotometry, Physics

Assignment Help:

Basic principles of spectrophotometry:

An absorbance spectrophotometer is an instrument that measures the fraction of the incident light transmitted through a solution.  In other words, it is used to measure the amount of light that passes through a sample material and, by comparison to the initial intensity of light reaching the sample, they indirectly measure the amount of light absorbed by that sample.

Spectrophotometers are designed to transmit light of narrow wavelength ranges (see Figure 1 the electromagnetic spectrum).  A given compound will not absorb all wavelengths equally-that's why things are different colors (some compounds absorb only wavelengths outside of the visible light spectrum, and that's why there are colorless solutions like water).  Because different compounds absorb light at different wavelengths, a spectrophotometer can be used to distinguish compounds by analyzing the pattern of wavelengths absorbed by a given sample.  Additionally, the amount of light absorbed is directly proportional to the concentration of absorbing compounds in that sample, so a spectrophotometer can also be used to determine concentrations of compounds in solution. Finally, because particles in suspension will scatter light (thus preventing it from reaching the light detector), spectrophotometers may also be used to estimate the number of cells in suspension.

We will be using a spectrophotometer several times this semester to quantify the concentration of chemicals present in a solution.

 

922_Basic principles of spectrophotometry.png

 


Related Discussions:- Basic principles of spectrophotometry

What are harmful effects of noise, Harmful effects of Noise: 1. Noise c...

Harmful effects of Noise: 1. Noise causes nervous tension, aggression and commotion in normal human beings. 2. Continuous noise damage hearing power and complete deafness.

Presbyopia, Presbyopia: The accommodation power of a person is lost by ...

Presbyopia: The accommodation power of a person is lost by which the suffers a long sightedness. It is known as Presbyopia or lack of accommodation.

Derivative of the sine function, Derivative of the Sine Function Extend...

Derivative of the Sine Function Extending a tradition of complex interrelations between the trigonometric functions, it turns out in which the derivative of the sine function i

emission spectrum, Emission spectrum of hidrogen is different from emissio...

Emission spectrum of hidrogen is different from emission spectrum of sodium.Why? Ans) Because, in sodium atom there are already many other electrons present in the atom aother than

Speed of center of oa just after collision, Two identical rods OA and OB ea...

Two identical rods OA and OB each of similar length and mass are connected by a massless pin connection, which permits rotation.The system is placed on a horizontal surface.Two mas

Power ratings of dc circuit, Power ratings of DC Circuit: Electrical eq...

Power ratings of DC Circuit: Electrical equipment can only stand a certain amount of heat production without damage and the safe power which a piece of equipment can consume wi

Evaluate the resonance frequency, The forced harmonic oscillations have sam...

The forced harmonic oscillations have same displacement amplitude at frequencies ω 1 = 400 Hz and ω 2 = 800 Hz. Evaluate the resonance frequency at which the displacement is maxi

Are end fitting standard, Are end fitting standard? No, end fitting are...

Are end fitting standard? No, end fitting are not standard. Each system manufacturer favors a type of termination for his fibers along with threads, pressure glands, rivets or

What is the meaning of polarization, What is the meaning of Polarization? ...

What is the meaning of Polarization? Polarization is a quality that is only exhibited by transverse waves. For light waves, the polarization identifies the direction of vibrati

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd