Basic principles of spectrophotometry, Physics

Assignment Help:

Basic principles of spectrophotometry:

An absorbance spectrophotometer is an instrument that measures the fraction of the incident light transmitted through a solution.  In other words, it is used to measure the amount of light that passes through a sample material and, by comparison to the initial intensity of light reaching the sample, they indirectly measure the amount of light absorbed by that sample.

Spectrophotometers are designed to transmit light of narrow wavelength ranges (see Figure 1 the electromagnetic spectrum).  A given compound will not absorb all wavelengths equally-that's why things are different colors (some compounds absorb only wavelengths outside of the visible light spectrum, and that's why there are colorless solutions like water).  Because different compounds absorb light at different wavelengths, a spectrophotometer can be used to distinguish compounds by analyzing the pattern of wavelengths absorbed by a given sample.  Additionally, the amount of light absorbed is directly proportional to the concentration of absorbing compounds in that sample, so a spectrophotometer can also be used to determine concentrations of compounds in solution. Finally, because particles in suspension will scatter light (thus preventing it from reaching the light detector), spectrophotometers may also be used to estimate the number of cells in suspension.

We will be using a spectrophotometer several times this semester to quantify the concentration of chemicals present in a solution.

 

922_Basic principles of spectrophotometry.png

 


Related Discussions:- Basic principles of spectrophotometry

Numbers, John is traveling north at 20 meters/second and his friend Betty ...

John is traveling north at 20 meters/second and his friend Betty is traveling south at 20 meters/second. If north is the positive direction, what are John and Betty''s speeds?

Kinematics, An aereplane has velocity 500m/s with respect to air. Wind blow...

An aereplane has velocity 500m/s with respect to air. Wind blows with velocity 20m/s along north. Then in what direction pilot should be heading so as to go along North-east direct

What is a fiber optic system, What is a fiber optic system? This term c...

What is a fiber optic system? This term comprises all the essential elements to install a working unit.

The proton and neutron are collectively called, The proton and neutron are ...

The proton and neutron are collectively called as: (1) Deutron (2) Positron (3) Meson (4) Nucleon Ans: (4) Nucleon

Determine the highest order spectrum, Determine the highest order spectrum ...

Determine the highest order spectrum which can be seen with monochromatic light of 589.3 nm wavelength by means of a diffraction grating having 15000 lines/inch.

Angular Momentum, In the experiment, under which of the following circumsta...

In the experiment, under which of the following circumstances is the angular momentum L of the movable puck NOT conserved? a) The net torque  = 0. b) The radius r is always perpen

Thermo and Statistical Dynamics, I need help with a problem. Must find th...

I need help with a problem. Must find the entropy S(E) in the microcanonical ensemble for this system for this system in terms of E and L(and the constants c,k,h(naught) and the

Waves, How can you measure wavelength in centimeters using a ruler?

How can you measure wavelength in centimeters using a ruler?

TRANSFORMERS, PRINCIPLES OF OPERATING DEPLETION MOSFET

PRINCIPLES OF OPERATING DEPLETION MOSFET

Find the electric potential, Q. Find the electric potential at a distance 0...

Q. Find the electric potential at a distance 0.09 m from a charge of 4 X 10 -7 C. The electric potential V = ( 1/4πεo ) q /r = ( 9 X 10 9 X 4 X 10 -7 )/ 9 X 10 -2 = 4 X 10

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd