Basic principles of spectrophotometry, Physics

Assignment Help:

Basic principles of spectrophotometry:

An absorbance spectrophotometer is an instrument that measures the fraction of the incident light transmitted through a solution.  In other words, it is used to measure the amount of light that passes through a sample material and, by comparison to the initial intensity of light reaching the sample, they indirectly measure the amount of light absorbed by that sample.

Spectrophotometers are designed to transmit light of narrow wavelength ranges (see Figure 1 the electromagnetic spectrum).  A given compound will not absorb all wavelengths equally-that's why things are different colors (some compounds absorb only wavelengths outside of the visible light spectrum, and that's why there are colorless solutions like water).  Because different compounds absorb light at different wavelengths, a spectrophotometer can be used to distinguish compounds by analyzing the pattern of wavelengths absorbed by a given sample.  Additionally, the amount of light absorbed is directly proportional to the concentration of absorbing compounds in that sample, so a spectrophotometer can also be used to determine concentrations of compounds in solution. Finally, because particles in suspension will scatter light (thus preventing it from reaching the light detector), spectrophotometers may also be used to estimate the number of cells in suspension.

We will be using a spectrophotometer several times this semester to quantify the concentration of chemicals present in a solution.

 

922_Basic principles of spectrophotometry.png

 


Related Discussions:- Basic principles of spectrophotometry

Series combination of the three resistors, Regard as three resistors having...

Regard as three resistors having three unique values of resistance. Presume the resistors are connected in series with each other. Additional suppose that the series combination of

Draw a free-body diagram for the space shuttle, Draw a free-body diagram fo...

Draw a free-body diagram for the space shuttle just after it leaves the ground.  Recognize the forces acting on the shuttle. Make sure that you do not neglect air resistance.

Question about antimatter, Hi i am doing research right now on anti-matter....

Hi i am doing research right now on anti-matter. I am just wondering if it is present in the human body?

Define gas in harmonic well, Define Gas in Harmonic Well? A classical ...

Define Gas in Harmonic Well? A classical system of N distinguishable no interacting particles of mass m is placed in a three-dimensional harmonic well: a) Discover t

Circular motion, In an amusement park ride called The Roundup, passengers s...

In an amusement park ride called The Roundup, passengers stand inside a 19.0 -diameter rotating ring. After the ring has acquired sufficient speed, it tilts into a vertical plane.

Describe the magnetic moment of a bar magnet, Describe the magnetic moment ...

Describe the magnetic moment of a bar magnet. What is its direction? Can you find two magnetic field lines intersecting? Why?

What are the various energy losses of a transformer, Q. What are the variou...

Q. What are the various energy losses of a transformer? Energy losses in a transformer: (a) Hysteresis loss (b Copper loss (c) Eddy current loss (Iron loss) (d)

Spectrum, calour formed in pure and impure spectrum

calour formed in pure and impure spectrum

Conventional current, Conventional Current: Earlier scientists had no k...

Conventional Current: Earlier scientists had no knowledge of the concept of electron, so the developed the convention, still in use, , that the direction of current is for appo

State electric field lines in good metallic conductors, Electric field line...

Electric field lines In good metallic conductors: In good metallic conductors:  (i)   Static electric fields are not there (zero inside a conductor) (ii)  Charges reside

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd