Basic principles of spectrophotometry, Physics

Assignment Help:

Basic principles of spectrophotometry:

An absorbance spectrophotometer is an instrument that measures the fraction of the incident light transmitted through a solution.  In other words, it is used to measure the amount of light that passes through a sample material and, by comparison to the initial intensity of light reaching the sample, they indirectly measure the amount of light absorbed by that sample.

Spectrophotometers are designed to transmit light of narrow wavelength ranges (see Figure 1 the electromagnetic spectrum).  A given compound will not absorb all wavelengths equally-that's why things are different colors (some compounds absorb only wavelengths outside of the visible light spectrum, and that's why there are colorless solutions like water).  Because different compounds absorb light at different wavelengths, a spectrophotometer can be used to distinguish compounds by analyzing the pattern of wavelengths absorbed by a given sample.  Additionally, the amount of light absorbed is directly proportional to the concentration of absorbing compounds in that sample, so a spectrophotometer can also be used to determine concentrations of compounds in solution. Finally, because particles in suspension will scatter light (thus preventing it from reaching the light detector), spectrophotometers may also be used to estimate the number of cells in suspension.

We will be using a spectrophotometer several times this semester to quantify the concentration of chemicals present in a solution.

 

922_Basic principles of spectrophotometry.png

 


Related Discussions:- Basic principles of spectrophotometry

Evaluate simple harmonic motion, Harmonicmotion is considered simple if it ...

Harmonicmotion is considered simple if it is undamped, i.e. if it keep on to oscillate uniformly over time. Of particular interest are the frequency f or also period T of the oscil

Snell''s law, explain one page for snell''s law?

explain one page for snell''s law?

Define term practical optical fibre in short, Define term practical optical...

Define term practical optical fibre in short. Optical fibre: A practical optical fibre has in common three coaxial areas. The innermost area is the light guiding region term

Explain vectors, Explain  vectors Vectors : a quantity that has a siz...

Explain  vectors Vectors : a quantity that has a size as well as direction is known a vector. So, for example, the wind blows with some speed and in some direction. So the win

Describe motion in two dimensions, Motion of an object in a plane is called...

Motion of an object in a plane is called two dimensional (2-D) motions. For 2-D motion acceleration or velocity can be explained by two elements in any two mutually perpendicular d

Microprocessor, Microprocessor A combination of IC's cal led a micropr...

Microprocessor A combination of IC's cal led a microprocessor. The data is processed in the microprocessor according to the instructions given through the input devices.  A

SI unit, what is the importance of si unit

what is the importance of si unit

Caluclate the work done by machine, A certain machine exerts a force of 200...

A certain machine exerts a force of 200 Newton on a box whose masses 30 kilograms.  The machine moves the box a distance of 20 meters with a horizontal floor. What amount o

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd