Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Basic principles of spectrophotometry:
An absorbance spectrophotometer is an instrument that measures the fraction of the incident light transmitted through a solution. In other words, it is used to measure the amount of light that passes through a sample material and, by comparison to the initial intensity of light reaching the sample, they indirectly measure the amount of light absorbed by that sample.
Spectrophotometers are designed to transmit light of narrow wavelength ranges (see Figure 1 the electromagnetic spectrum). A given compound will not absorb all wavelengths equally-that's why things are different colors (some compounds absorb only wavelengths outside of the visible light spectrum, and that's why there are colorless solutions like water). Because different compounds absorb light at different wavelengths, a spectrophotometer can be used to distinguish compounds by analyzing the pattern of wavelengths absorbed by a given sample. Additionally, the amount of light absorbed is directly proportional to the concentration of absorbing compounds in that sample, so a spectrophotometer can also be used to determine concentrations of compounds in solution. Finally, because particles in suspension will scatter light (thus preventing it from reaching the light detector), spectrophotometers may also be used to estimate the number of cells in suspension.
We will be using a spectrophotometer several times this semester to quantify the concentration of chemicals present in a solution.
Get a mathematical expression for current and impedance in series LCR circuit. Reduce an expression for the resonating frequency of an LCR series resonating circuit.
A small bulb is placed at the bottom of a tank containing water to a depth of 80 cm. What is the area of the surface of water through which light from the bulb can emerge out? Refr
Resolution of Forces: This topic is important, but is really the opposite to Addition of forces. Recalling that two forces can be added to give a single force known
A car of mass 1600 kilograms (kg) was travelling at 30m/s when the brakes were applied and produced a constant retarding force of 8000 N (Newtons) to the car: Find - a) How far the
Figure denotes a rod whose ends are in thermal contact with a hot reservoir at temperature T1 and a cold reservoir at temperature T2. The ends of the rod are covered with insulati
According to Reynolds, the critical velocity (vc) of a liquid flowing by a long narrow tube is (a) Directly linked to the coefficient of viscosity of the liquid. (b) Inversel
Displacement: - The modification in position of a body in a certain direction is known as displacement. It is a vector function and its unit in SI system is meter.
how can we conclude whether at some time interference will take place or not with the help of the concept of temporal coherence?
Carnot's theorem (S. Carnot) The theorem which states that no engine operating among two temperatures can be more capable than a reversible engine.
Color vision and chromatic aberration: One of the remarkable ability of the eye is its ability to see color. There are three types of cones that respond to light from three
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd