Basic principles of spectrophotometry, Physics

Assignment Help:

Basic principles of spectrophotometry:

An absorbance spectrophotometer is an instrument that measures the fraction of the incident light transmitted through a solution.  In other words, it is used to measure the amount of light that passes through a sample material and, by comparison to the initial intensity of light reaching the sample, they indirectly measure the amount of light absorbed by that sample.

Spectrophotometers are designed to transmit light of narrow wavelength ranges (see Figure 1 the electromagnetic spectrum).  A given compound will not absorb all wavelengths equally-that's why things are different colors (some compounds absorb only wavelengths outside of the visible light spectrum, and that's why there are colorless solutions like water).  Because different compounds absorb light at different wavelengths, a spectrophotometer can be used to distinguish compounds by analyzing the pattern of wavelengths absorbed by a given sample.  Additionally, the amount of light absorbed is directly proportional to the concentration of absorbing compounds in that sample, so a spectrophotometer can also be used to determine concentrations of compounds in solution. Finally, because particles in suspension will scatter light (thus preventing it from reaching the light detector), spectrophotometers may also be used to estimate the number of cells in suspension.

We will be using a spectrophotometer several times this semester to quantify the concentration of chemicals present in a solution.

 

922_Basic principles of spectrophotometry.png

 


Related Discussions:- Basic principles of spectrophotometry

Determine the forbidden gap of germanium, For germanium the forbidden gap i...

For germanium the forbidden gap is (A) 0.15ev. (B) 0.25ev. (C) 0.5ev. (D) 0.7ev. Ans: For germanium the forbidden gap is 0.7ev.

Why isn''t copper used as a heating element?, It makes sense that copper sh...

It makes sense that copper should be used because it could heat up faster, making the event more efficient.

Heat equation + matlab, I need to solve and write a MATLAB code the heat eq...

I need to solve and write a MATLAB code the heat equation by two methods ( Crank-Nicolson method and asymptotic method )

What is angle of dispersion in a prism, I think it is same as the apex angl...

I think it is same as the apex angle of the prism or the angle of the prism. since it decides the angle of deviation as i+e=D+A(angle of the prism),D=deviation.

Compute the area moment of inertia, Use the numerical integration scheme di...

Use the numerical integration scheme discussed in class to compute the area moment of inertia of the circular section with a hole as shown below.  For mapping, use 4-node element

Electric and magnetic phenomena, The line density of charge in a wire is 0....

The line density of charge in a wire is 0.20 Cm-1. If this wire is symmetrically enclosed along its length by a cylindrical surface of radius 1.0 m and length 1.5 m, calculate

Fermi paradox, Fermi paradox (E. Fermi): E. Fermi's conjecture, simpli...

Fermi paradox (E. Fermi): E. Fermi's conjecture, simplified along phrase, "Where are they?" questioning that if the Galaxy is filled along with technological and intelligent c

What is superconductivity, Q. What is superconductivity? The capability...

Q. What is superconductivity? The capability of certain metals their compounds and alloys to conduct electricity with zero resistance at very low temperatures are called superc

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd