Basic principles of spectrophotometry, Physics

Assignment Help:

Basic principles of spectrophotometry:

An absorbance spectrophotometer is an instrument that measures the fraction of the incident light transmitted through a solution.  In other words, it is used to measure the amount of light that passes through a sample material and, by comparison to the initial intensity of light reaching the sample, they indirectly measure the amount of light absorbed by that sample.

Spectrophotometers are designed to transmit light of narrow wavelength ranges (see Figure 1 the electromagnetic spectrum).  A given compound will not absorb all wavelengths equally-that's why things are different colors (some compounds absorb only wavelengths outside of the visible light spectrum, and that's why there are colorless solutions like water).  Because different compounds absorb light at different wavelengths, a spectrophotometer can be used to distinguish compounds by analyzing the pattern of wavelengths absorbed by a given sample.  Additionally, the amount of light absorbed is directly proportional to the concentration of absorbing compounds in that sample, so a spectrophotometer can also be used to determine concentrations of compounds in solution. Finally, because particles in suspension will scatter light (thus preventing it from reaching the light detector), spectrophotometers may also be used to estimate the number of cells in suspension.

We will be using a spectrophotometer several times this semester to quantify the concentration of chemicals present in a solution.

 

922_Basic principles of spectrophotometry.png

 


Related Discussions:- Basic principles of spectrophotometry

What do you mean by thermoelectric refrigerators, What is the specific resi...

What is the specific resistance of material of a conductor. What do you mean by thermoelectric refrigerators?

Why x-rays are not used in microscopes, Q. Why X-rays are not used in micro...

Q. Why X-rays are not used in microscopes? 1) The wavelength of X-rays is slighter than that of the visible light. 2) X-rays can't be focussed as visible radiations are foc

Largest acceptable prostate volume cubic centimeters, Prof. Patch is collab...

Prof. Patch is collaborating with physicians at MCW on an ex vivo prostate cancer imaging trial. The testbed they currently use is 6 cm wide. The research nurse coordinating the

Briefly explain brewsters law, Briefly explain Brewster's law? Show that at...

Briefly explain Brewster's law? Show that at the polarizing angle of incidence the reflected and transmitted light becomes perpendicular to each other.

Chemistry in action - detergents, chemistry in action - Detergents A re...

chemistry in action - Detergents A result of high dissolving power, the logically happening water all time consists of dissolved matters, majorly ionic substances. Hard water h

Difference between an electron and a beta ray, What is the essential diffe...

What is the essential difference between an electron and a beta ray? The electron of nuclear origin is known as a beta-particle. There is otherwise no difference among an elec

Principles of radiation therapy, Principles of radiation therapy: The ...

Principles of radiation therapy: The basic principle of radiation therapy is to maximize damage to the tumor while minimizing damage to the normal tissue.Factors that determin

Universal constant of gravitation, Universal constant of gravitation; G: ...

Universal constant of gravitation; G: The constant of proportionality in Newton's law of universal gravitation & which plays an vital role in A. Einstein's general relativity.

Electricity, what is the use of the muslin bag in a dry cell

what is the use of the muslin bag in a dry cell

Factors affecting capacitance, Factors affecting capacitance: The facto...

Factors affecting capacitance: The factors which affect the capacitance of a parallel-plate capacitor are: Overlapping area of the plates (A). The capacitance increases

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd