Basic principles of spectrophotometry, Physics

Assignment Help:

Basic principles of spectrophotometry:

An absorbance spectrophotometer is an instrument that measures the fraction of the incident light transmitted through a solution.  In other words, it is used to measure the amount of light that passes through a sample material and, by comparison to the initial intensity of light reaching the sample, they indirectly measure the amount of light absorbed by that sample.

Spectrophotometers are designed to transmit light of narrow wavelength ranges (see Figure 1 the electromagnetic spectrum).  A given compound will not absorb all wavelengths equally-that's why things are different colors (some compounds absorb only wavelengths outside of the visible light spectrum, and that's why there are colorless solutions like water).  Because different compounds absorb light at different wavelengths, a spectrophotometer can be used to distinguish compounds by analyzing the pattern of wavelengths absorbed by a given sample.  Additionally, the amount of light absorbed is directly proportional to the concentration of absorbing compounds in that sample, so a spectrophotometer can also be used to determine concentrations of compounds in solution. Finally, because particles in suspension will scatter light (thus preventing it from reaching the light detector), spectrophotometers may also be used to estimate the number of cells in suspension.

We will be using a spectrophotometer several times this semester to quantify the concentration of chemicals present in a solution.

 

922_Basic principles of spectrophotometry.png

 


Related Discussions:- Basic principles of spectrophotometry

What are other manufactured wavelengths in helium-neon laser, What are othe...

What are other manufactured wavelengths in Helium-Neon Laser? Then what remembers while there is mention of a He-Ne laser is a red beam, those along with other wavelengths are

Electricity, What does it mean when a coulomb of charge has a certain numbe...

What does it mean when a coulomb of charge has a certain number of joules?

Calculate the fiber volume fraction, An engineer is using DSC to determine ...

An engineer is using DSC to determine the fiber volume fraction of a carbon fiber / epoxy prepreg. It is known that the heat of reaction of the pure, fresh epoxy resin (no carbon

Explain two-stage rocket, Two Stage Rocket Suppose the motion of the tw...

Two Stage Rocket Suppose the motion of the two-stage rocket or the corresponding velocity-time graph below. The rocket has two successive fuel stages followed through a free-fa

PVD, In order to deposit films of the alloy YBa2Cu3, the metals Y, Ba, and ...

In order to deposit films of the alloy YBa2Cu3, the metals Y, Ba, and Cu are evaporated from three point sources. The latter are situated at the corners of an equilateral triangle

Explain the basic characteristics of grating spectra, Explain the basic cha...

Explain the basic characteristics of grating spectra?

Kinametic, whatis meant by kinametic?

whatis meant by kinametic?

Define poisson distribution in ideal gas, Define Poisson Distribution in Id...

Define Poisson Distribution in Ideal Gas? Consider a monatomic ideal gas of total Ñ molecules in a volume V ˜ Show that the probability, P N for the number N of mole

Flux of electric field strength vector across the circle , Two point charge...

Two point charges q and -q are splitted by distance 2l. Find the flux of electric field strength vector across the circle of radius R placed with its centre coinciding with the cet

what percentage should the pressure of a given mass, By what percentage sh...

By what percentage should the pressure of a given mass of gas be increased,so as to decrease its volume  by 10% at a constant temperature.

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd