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Bacteriophage is a virus which infects a bacterium and which is many times used in molecular genetics experiments as the vector, or cloning vehicle. Recombinant phages can be made in which certain non-essential l DNA is removed and then replaced with the DNA of interest. The phage can accommodate the DNA "insert" of about 15-20 kb. Replication of that virus will hence replicate the investigator's DNA. One would take use of phage l rather than a plasmid if the desired piece of DNA is rather large. Bacteriophage l and M13 are the ones usually used in cloning or subcloning of small genes or DNA fragments in E. coli. Bacteriophage P1 is one which is used for fragments up to 95 Kb in size.
Q. Show overall management of diabetes? Diabetes as we all know is a disease with ever increasing prevalence in' our country. It can be described as a disease characterized by
C a n d i d i a s i s (moniliasis or thrush) C and i d a albicans , the causative fungus of the disease, is widespread in the environment and is usually present
PHYSIOLOGY OF EXCRETION - 1 . FORMATION OF UREA - It takes place in liver. (i) Deamination (ii) Ornithine cycle or urea cycle - Explained by H.
Compare between ISP and soy flour The cost of isolated soybean proteins is five to seven times higher than that of defatted soy flour. On an equivalent protein weight basis, th
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Salient Features of pulmonary tuberculosis The salient features tuberculosis include: Wasting of tissues Exhaustion Cough Expectoration, and Fever The acute p
Phenylketonuria is a severe form of mental retardation caused by a recessive allele. The condition affects about 1 in 25,000 newborn babies. In a population of 250,000 people, how
Neuron A is a healthy neuron with all the usual ion channels. When at rest with a membrane voltage of R millivolts, neuron A produces no action potentials. The voltage thresh
you are setting up a pcr reaction to amplify a 1000 basepair long dna grament. in your 50 microliter reaction volume you have 100 nanogrM OD RWMPLrw sn QIRH eoufly 50/50 at/gc 100
Define Factors Which May Lead To Errors in Count? There are many factors, which may lead to errors in count. (i) Counts may be low if the agar medium used in the technique d
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