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Availability of ADP
When the ADP levels increase due to hydrolysis ofATP in various biosynthetic reactions, the rate of reaction to generate ATP is accelerated and this is mainly by oxidative phosphorylation. There are 4 reactions in which the reducing equivalents are transported to respiratory chain coupled with the generation ofATP in this cycle and thus increase in ADP causes oxidation of acetyl CoA by the citric acid cycle.
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Pyruvate carboxylation Pyruvate carboxylation : In this reaction, pyruvate, CO, and ATP are converted to oxaloacetate, ADP and Pi catalysed by the enzymepyruvate carboxylase
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