Autoradiography, Biology

Assignment Help:

Autoradiography is the process to detect radioactively labeled molecules (which commonly have been separated in an SDS-PAGE or agarose gel) based on their ability to develop an image on photographic or X-ray film. This procedure does not result in a linear relationship between the intensity of the signal and the amount of the radioactivity unless special steps are taken. There is now increasing use of the phosphorimagers and other modern devices to detect and quantitative radioactive molecules which have been separated in gels.


Related Discussions:- Autoradiography

Explain relative quantitative difference of protein taxonomy, Explain Relat...

Explain Relative Quantitative difference of Protein Taxonomy This analysis involves the study between the amounts of different proteins and more significantly, of different con

Illustrate erythropoietin, Define Erythropoietin A. acts by stimulating...

Define Erythropoietin A. acts by stimulating the production of red blood cells by the peritubular interstitial cells of the kidney cortex. B. is secreted by cells in the bon

Explain energy requirement in postoperative nutritional care, Explain the E...

Explain the Energy requirement in Postoperative Nutritional Care? Adequate calorie intake is critical for successful outcome of surgery and should be provided in the form of ca

Explain about chthalamus, Explain about chthalamus The realised niche o...

Explain about chthalamus The realised niche of Chthalamus is reduced from its fundamental niche and it is limited to the upper tidal zone Lower  limits Out-competed

What would be in the solution, Before you solve the problem organize your t...

Before you solve the problem organize your thoughts on paper in the following manner. A) What will my answer look like? What units will I be using? B) What do I need to know

Explain restricted fragment polymorphism, Q. Explain Restricted fragment po...

Q. Explain Restricted fragment polymorphism? This method makes use of a group of enzymes termed restriction enzymes which recognise particular sequence of DNA and act as "molec

Why do cells of the nephron tubules, Q. Why do cells of the nephron tubules...

Q. Why do cells of the nephron tubules present a great amount of mitochondria? The cells of the tubule wall have high number of mitochondria because many substances are secrete

What is vertebrates, What is Vertebrates ? Vertebrates are animals that...

What is Vertebrates ? Vertebrates are animals that have vertebral columns, and include the following classes: Agnatha - jawless fishes (ex. lampreys) Chrondirichthyes - cart

Name three major groups into which mammals are divided, What are the three ...

What are the three major groups into which mammals are divided? The three groups into which mammals are divided are: monotremes (or prototherian, e.g., platypus), marsupials (o

Define glycogen , A glycogen molecule having entirely of glucose uni...

A glycogen molecule having entirely of glucose units, many of that are linked in long chains through α1-4 bonds.  Furthermore,  every  10  units  or  so,  the  chain  is branched

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd