Autoradiography, Biology

Assignment Help:

Autoradiography is the process to detect radioactively labeled molecules (which commonly have been separated in an SDS-PAGE or agarose gel) based on their ability to develop an image on photographic or X-ray film. This procedure does not result in a linear relationship between the intensity of the signal and the amount of the radioactivity unless special steps are taken. There is now increasing use of the phosphorimagers and other modern devices to detect and quantitative radioactive molecules which have been separated in gels.


Related Discussions:- Autoradiography

Define the physical activity level, Define the Physical activity level? ...

Define the Physical activity level? Physical activity level (PAL) is defined as the total energy required over 24 hours divided by the energy needed for basal metabolism over 2

Show the energy pyramids, Q. Can the amount of available energy in a given ...

Q. Can the amount of available energy in a given trophic level be larger than the available energy in inferior trophic levels? What does that condition means to the conformation of

Define primary level care - public nutrition, Define Primary Level Care - P...

Define Primary Level Care - Public Nutrition? This is the first level of contact of an individual, the family and the community with the national health system. It is possible

Polyinvagination or ingression, PO L YIN V AGIN A TIO N OR INGRESSIO...

PO L YIN V AGIN A TIO N OR INGRESSION - This is found in discoblastula and periblastula. In it the blastomeres of the blastoderm divide and form new blastomeres wh

Procedures for diagnosis - demonstration of specific antigen, Demonstration...

Demonstration of specific antigen(s): The presence of the viral antigen in the infected tissues and cells can be demonstrated by fluorescent antibody technique (FAT) and

Standard titration - estimation of vitamin c in lemon juice, Explain Standa...

Explain Standard titration - Estimation of Vitamin C in Lemon Juice? Pipette 5 ml of standard ascorbic acid solution into a 100 ml conical flask. Fill the burette with the dye s

What are the uses of national parks, Q. What are the uses of National parks...

Q. What are the uses of National parks ? National Park is an area dedicated to conserve environment, natural and historical objects and to conserve the wild life therein. Natio

Consequences on the bony structures, Q. Consequences on the bony structures...

Q. Consequences on the bony structures? Basal bone forms the dental skeletal structure. Wolff's law states that the bone remodels in relationship to the forces applied. With a

Technique-recurrence of angina, Technique:  Careful re-opening of ste...

Technique:  Careful re-opening of sternum and release of steinum from the underlying adhesion is done. Femoral artery and vein are exposed for going on femoro fenioral bypass

Green glands of crustaceans, Normal 0 false false false ...

Normal 0 false false false EN-IN X-NONE X-NONE MicrosoftInternetExplorer4

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd