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Autoradiography
Autoradiography is a modification of the radioactive tracer technique. In this technique, the radioactivity is detected by a thin film of photographic emulsion containing silver compounds which is put over a labelled cell or tissue. The rays emitted by radioisotope from the cell or tissue expose the photographic emulsion like a photographic film which is developed in a dark room. Silver grains are seen as black dots on parts of the specimen where radioisotope is accumulated.
Autoradiography reveals the metabolic processes of the cell. For example, by labelling cells with 3H thymidine, it is found that silver grains are localised in nucleus, which shows that DNA is synthesised in the nucleus. Similarly, by labelling cells with 3H uridine, silver grains are first seen in nucleus and then after some time these grains appear in the cytoplasm. This clearly demonstrates that RNA is initially synthesised in the nucleus but later on migrates to the cytoplasm.Different radioisotopes are used for localising different macromolecules. As stated earlier, cells are labelled with 3H thymidine for the studX of DNA metabolism, 3H uridine to study RNA metabolism, tritiated amino acids for examining the synthesis of proteins, treated monosaccharides such as 3H-mannose and 3H-fucose for examining the synthesis of polysaccharides.
Triacylglycerols which is also known as triglycerides or fats consist of three fatty acid chains esterified to a glycerol backbone. Simple triacylglycerols have t
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Q. What do you mean by Periodical? A periodical is a publication appearing at regular intervals. Each issue is called a number and collectively these numbers comprise a volume.
Indifferences in ionic composition Answer A. across membranes can be created through the action of ATP driven pumps as long as number of negative and positive ions on remain equal
Explain about Regenerative therapy Is also used to achieve the above objectives but with the ultimate goal of regeneration of lost bone tissue.
State the concept of type locus interaction Some of the major contributions included the concept of type locus interaction, the analysis of quantitative as opposed to qualitati
CHEMICAL CHANGES DURING CLEAVAGE - 1. Synthesis of DNA occurs. 2. Synthesis of RNA (rRNA is absent, mRNA & tRNA are synthesized very little amount). 3.
various parameters which are used in water quality measurement
Q. What is the life cycle of a tapeworm? The Pregnant proglottids with taenia eggs are released together with human feces. If ingested by the intermediate hosts, bovineor swine
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