Autoradiography, Biology

Assignment Help:

Autoradiography

Autoradiography is a modification of the radioactive tracer technique. In this technique, the radioactivity is detected by a thin film of photographic emulsion containing silver compounds which is put over a labelled cell or tissue. The rays emitted by radioisotope from the cell or tissue expose the photographic emulsion like a photographic film which is developed in a dark room. Silver grains are seen as black dots on parts of the specimen where radioisotope is accumulated.

Autoradiography reveals the metabolic processes of the cell. For example, by labelling cells with 3H thymidine, it is found that silver grains are localised in nucleus, which shows that DNA is synthesised in the nucleus. Similarly, by labelling cells with 3H uridine, silver grains are first seen in nucleus and then after some time these grains appear in the cytoplasm. This clearly demonstrates that RNA is initially synthesised in the nucleus but later on migrates to the cytoplasm.

Different radioisotopes are used for localising different macromolecules. As stated earlier,  cells are labelled with 3H thymidine for the studX of DNA metabolism, 3H uridine to study RNA metabolism, tritiated amino acids for examining the synthesis of proteins, treated monosaccharides such as 3H-mannose and 3H-fucose for examining the synthesis of polysaccharides.


Related Discussions:- Autoradiography

Ichnology, Ichnology :  This is the study of foot prints. Ichnology is a ty...

Ichnology :  This is the study of foot prints. Ichnology is a type of branch of geology which deals with traces of organismal behavior like as burrows or footprints. It is commonly

Special characteristic of stem cells, What is the special characteristic of...

What is the special characteristic of (a) stem cells, (b) embryonic stem cells? (a) Stem cells can continue to divide throughout their life. (b)The special characteristic of

What are some diseases caused by abnormal gh secretion, What are some disea...

What are some diseases caused by abnormal GH secretion by the hypophysis? In childhood deficient GH secretion might be lead to delayed growth and in severe cases to nanism (dwa

What is genetic mapping, How can the concept of recombination frequency be ...

How can the concept of recombination frequency be used in genetic mapping? Genetic mapping is the determination of the location of the genes in a chromosome. By determining

Flatworms, What are the adaptions flatworms neeed in order to live in their...

What are the adaptions flatworms neeed in order to live in their environment?

Listeriosis, List er i o si s The disease is caused by Listeria m...

List er i o si s The disease is caused by Listeria monocytogenes - Gram positive small non spore- forming bacilli seen in soil, silage, sewage and feces of animals and b

Protozoa, What are the disadvantages of protozoa

What are the disadvantages of protozoa

Class of coclentrata, ????? # 100 ??????????? #Minimum ?????? ?????

????? # 100 ??????????? #Minimum ?????? ?????

Determine the viscosity of solutions, Viscosity Viscosity, as you may ...

Viscosity Viscosity, as you may already know, is associated with fluid flow.  It is the internal friction which tends to bring to rest portions of the fluids moving relative to

Hazards of oxygen therapy - respiratory failure, Hazards of O 2 Therapy ...

Hazards of O 2 Therapy Exposure to greater than 60 per cent O 2 , for a period of more than 36 hours, or exposure to 100 per cent 0, for a period of more than 6 hours, res

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd