Autoradiography, Biology

Assignment Help:

Autoradiography

Autoradiography is a modification of the radioactive tracer technique. In this technique, the radioactivity is detected by a thin film of photographic emulsion containing silver compounds which is put over a labelled cell or tissue. The rays emitted by radioisotope from the cell or tissue expose the photographic emulsion like a photographic film which is developed in a dark room. Silver grains are seen as black dots on parts of the specimen where radioisotope is accumulated.

Autoradiography reveals the metabolic processes of the cell. For example, by labelling cells with 3H thymidine, it is found that silver grains are localised in nucleus, which shows that DNA is synthesised in the nucleus. Similarly, by labelling cells with 3H uridine, silver grains are first seen in nucleus and then after some time these grains appear in the cytoplasm. This clearly demonstrates that RNA is initially synthesised in the nucleus but later on migrates to the cytoplasm.

Different radioisotopes are used for localising different macromolecules. As stated earlier,  cells are labelled with 3H thymidine for the studX of DNA metabolism, 3H uridine to study RNA metabolism, tritiated amino acids for examining the synthesis of proteins, treated monosaccharides such as 3H-mannose and 3H-fucose for examining the synthesis of polysaccharides.


Related Discussions:- Autoradiography

Explain treatment options for ailing and failing implants, Treatment option...

Treatment options for ailing and failing implants are varied. The clinician should start conservatively and progress to more aggressive therapy. The overall goal of therapy is

Intra cranial mycotic aneurysms, The reported occurrence of ICMAs is 1.2 pe...

The reported occurrence of ICMAs is 1.2 per cent to 5 per cent of cases. Streptococci and S.aureus account for 50 per cent and 10 per cent of cases, respectively, and are seen with

Explain phylum arthropoda, Phylum Arthropoda : (8,00,000 to 10,00,000 spec...

Phylum Arthropoda : (8,00,000 to 10,00,000 species) They are known as 'jointed footed' animals. The appendages are paired, and jointed with chitinous exoskeleton, varied and wi

Explain counseling children, Counseling children Assess the child's sta...

Counseling children Assess the child's stage of development. Adjust counseling for a child's  dependency needs,  lack of  experience and  the development tasks faced by  him

Explain the histologic techniques, Explain the histologic techniques A...

Explain the histologic techniques A clinician has to base the diagnosis only on the clinical and radiographic findings as microbiologic and histologic techniques are restricte

Define techniques for detection hidden root canals, Define Techniques for D...

Define Techniques for Detection Hidden Root Canals Three well-angulated periapical films for 3D morphology: o With the cone directed straight on. o Mesiobilque. o And disto

Natural and artificial ecosystem, Natural and Artificial Ecosystem i) N...

Natural and Artificial Ecosystem i) Natural ecosystems are those which are mostly free from human disturbances, such as tropical forests, grasslands, oceans, lakes and deserts.

Gametogenesis - human development, Gametogenesis - Human Development G...

Gametogenesis - Human Development Gametogenesis as you are responsive is the process of formation and development of specialized reproductive cells, ova in females and sperms

Define ebb or shock phase - physiological response to injury, Define early ...

Define early ebb or shock phase - Physiological Response to injury? This is usually brief in duration lasting 12 to 24 hours and occurs immediately following injury. Blood pres

Explain about the serum vitamin b12 assay, Explain about the Serum vitamin ...

Explain about the Serum vitamin B12 assay? Serum vitamin B 12 assay: The vitamin B 12 content of serum can be determined.  A serum level of 12 deficiency.

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd