Autoradiography, Biology

Assignment Help:

Autoradiography

Autoradiography is a modification of the radioactive tracer technique. In this technique, the radioactivity is detected by a thin film of photographic emulsion containing silver compounds which is put over a labelled cell or tissue. The rays emitted by radioisotope from the cell or tissue expose the photographic emulsion like a photographic film which is developed in a dark room. Silver grains are seen as black dots on parts of the specimen where radioisotope is accumulated.

Autoradiography reveals the metabolic processes of the cell. For example, by labelling cells with 3H thymidine, it is found that silver grains are localised in nucleus, which shows that DNA is synthesised in the nucleus. Similarly, by labelling cells with 3H uridine, silver grains are first seen in nucleus and then after some time these grains appear in the cytoplasm. This clearly demonstrates that RNA is initially synthesised in the nucleus but later on migrates to the cytoplasm.

Different radioisotopes are used for localising different macromolecules. As stated earlier,  cells are labelled with 3H thymidine for the studX of DNA metabolism, 3H uridine to study RNA metabolism, tritiated amino acids for examining the synthesis of proteins, treated monosaccharides such as 3H-mannose and 3H-fucose for examining the synthesis of polysaccharides.


Related Discussions:- Autoradiography

What is excessive fat intake, What is Excessive Fat Intake? Although mi...

What is Excessive Fat Intake? Although minimum amount of fat is essential as calculated above but upper limit of fat should be drawn due to its link with an increased risk of d

Phylum protozoa, What is the classification of this phylum

What is the classification of this phylum

Why plant cycle know as alternation of generations, Q. Why is the plant lif...

Q. Why is the plant life cycle known as alternation of generations? The plant life cycle is called as alternation of generations because in this cycle there are two different f

Relevance of biology to agriculture, ways in which modern agricultural acti...

ways in which modern agricultural activities may threaten survival species

How sugar play a major role in fermentation, How sugar play a major role in...

How sugar play a major role in Fermentation? The production of chemicals by fermenting several sugars is a very well-accepted science. Its utilize ranges from producing beverag

Post disease as a result -endodontists, If the condition or the post diseas...

If the condition or the post disease as a result of intraradicular infection----> treated conventionally via apex " non-surgical ", ---->Here we have 2 options either coronal

Explain database search, Database Search: Once an open reading frame or th...

Database Search: Once an open reading frame or the partial amino acid sequence has been pre-determined, the investigator compares sequence with others in the databases using a com

Excretion, what are the excretory organs of birds

what are the excretory organs of birds

Explain gene expression - nutrient gene interactions, Explain Gene Expressi...

Explain Gene Expression - Nutrient Gene Interactions? The last two decades have witnessed tremendous development in our understanding of the cellular processes at the molecular

Which type of ecological interaction is competition, What is competition? W...

What is competition? Which type of ecological interaction is competition? Competition is the ecological interaction in which the individuals explore the similar ecological nich

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd