Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Autoradiography
Autoradiography is a modification of the radioactive tracer technique. In this technique, the radioactivity is detected by a thin film of photographic emulsion containing silver compounds which is put over a labelled cell or tissue. The rays emitted by radioisotope from the cell or tissue expose the photographic emulsion like a photographic film which is developed in a dark room. Silver grains are seen as black dots on parts of the specimen where radioisotope is accumulated.
Autoradiography reveals the metabolic processes of the cell. For example, by labelling cells with 3H thymidine, it is found that silver grains are localised in nucleus, which shows that DNA is synthesised in the nucleus. Similarly, by labelling cells with 3H uridine, silver grains are first seen in nucleus and then after some time these grains appear in the cytoplasm. This clearly demonstrates that RNA is initially synthesised in the nucleus but later on migrates to the cytoplasm.Different radioisotopes are used for localising different macromolecules. As stated earlier, cells are labelled with 3H thymidine for the studX of DNA metabolism, 3H uridine to study RNA metabolism, tritiated amino acids for examining the synthesis of proteins, treated monosaccharides such as 3H-mannose and 3H-fucose for examining the synthesis of polysaccharides.
i need to complete a model on loop analysis Java and answer questions on my assignment sheet....any experts to help me do my lab homework?
Q. How much DNA is in a typical human cell? If DNA (deoxyribonucleic acid) molecules in a single human cell were stretched out and laid end to end they will measure approximate
Disease occurrence due to climate change The movement of animal diseases across physical and political boundaries threatens food security and creates a global public co
Life cycle of malarial parasite When the mosquito sucks the blood, gametocytes enter its digestive system. They migrate into the walls of the digestive system and undergo
Give three examples of human disorders which are caused by the action of a single pair of alleles. In every case say whether the harmful allele is dominant or recessive to the non-
Types of xerophytes On the basis of their mbrphology, physiology and life cycle pattern, xerophytes are generally classified into the following three categories: a) Ephem
Ask quYour organs are wonderous things, each one with a different function vital to the homeostasis of your body. While it is easy for us to view a particular organ as a single ite
biostatistical application in biotechnology
How does the interaction between a carrier protein and the substance it transports resemble the interaction among an enzyme and its substrate? Both include the binding of a spe
Phases of viruses Viruses have two phases to their existence Inside their host cells where they exhibit certain living characteristics. outside their host where they
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd