Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Autoradiography
Autoradiography is a modification of the radioactive tracer technique. In this technique, the radioactivity is detected by a thin film of photographic emulsion containing silver compounds which is put over a labelled cell or tissue. The rays emitted by radioisotope from the cell or tissue expose the photographic emulsion like a photographic film which is developed in a dark room. Silver grains are seen as black dots on parts of the specimen where radioisotope is accumulated.
Autoradiography reveals the metabolic processes of the cell. For example, by labelling cells with 3H thymidine, it is found that silver grains are localised in nucleus, which shows that DNA is synthesised in the nucleus. Similarly, by labelling cells with 3H uridine, silver grains are first seen in nucleus and then after some time these grains appear in the cytoplasm. This clearly demonstrates that RNA is initially synthesised in the nucleus but later on migrates to the cytoplasm.Different radioisotopes are used for localising different macromolecules. As stated earlier, cells are labelled with 3H thymidine for the studX of DNA metabolism, 3H uridine to study RNA metabolism, tritiated amino acids for examining the synthesis of proteins, treated monosaccharides such as 3H-mannose and 3H-fucose for examining the synthesis of polysaccharides.
Normal 0 false false false EN-IN X-NONE X-NONE MicrosoftInternetExplorer4
Q. What is cross contamination? Cross contamination is the passage of micro-organisms from one person to another via any route direct or indirect.
Q. Procedures Adopted by Numerical Taxonomists? Since numerical taxonomy is an operational science, the procedure is divided into a number of repeatable steps, allowing the res
Q. How do fishes do gas exchange? Fishes "breath" through gills, branchiae or Gills, are highly vascularized organs specialized in gas exchange under water and present in aquat
Omnispective Classification This is the extension of the concept of natural classification put forward by Black Welder (1967). The approach seems quite realistic and pragmatic.
Composition of agar Agar we learnt consists of a mixture of agrose and agropectin. Agrose has a linear polymer structure consisting of alternating D-galactose and 3,6-anhydro
What is the significance of Pharyngeal gill slit ? Lateral opening in the wall of the pharynx which allows water to enter into the mouth and exit through the pharynx. This is on
A disulfide bond can form between any two amino acids that have sulfur in their side-chain.
What are mutagenic agents? The Mutagenic agents, or mutagens, are physical, chemical or biological factors that can cause alteration in DNA molecules. Instances of believed
Enumerate the term - pathognomic sign A comprehensive assessment ideally uses all four approaches. Some forms of specialised assessment rely heavily on pathognomic signs, behav
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd