Autoradiography, Biology

Assignment Help:

Autoradiography

Autoradiography is a modification of the radioactive tracer technique. In this technique, the radioactivity is detected by a thin film of photographic emulsion containing silver compounds which is put over a labelled cell or tissue. The rays emitted by radioisotope from the cell or tissue expose the photographic emulsion like a photographic film which is developed in a dark room. Silver grains are seen as black dots on parts of the specimen where radioisotope is accumulated.

Autoradiography reveals the metabolic processes of the cell. For example, by labelling cells with 3H thymidine, it is found that silver grains are localised in nucleus, which shows that DNA is synthesised in the nucleus. Similarly, by labelling cells with 3H uridine, silver grains are first seen in nucleus and then after some time these grains appear in the cytoplasm. This clearly demonstrates that RNA is initially synthesised in the nucleus but later on migrates to the cytoplasm.

Different radioisotopes are used for localising different macromolecules. As stated earlier,  cells are labelled with 3H thymidine for the studX of DNA metabolism, 3H uridine to study RNA metabolism, tritiated amino acids for examining the synthesis of proteins, treated monosaccharides such as 3H-mannose and 3H-fucose for examining the synthesis of polysaccharides.


Related Discussions:- Autoradiography

What are the antigens and antibodies of abo blood system, What are the anti...

What are the antigens and antibodies of each blood type of the ABO blood system? Type A: antigen A, antibody anti-B. Type B: antigen B, antibody anti-A. Type AB: antigens A and

Which part of human brain is relay station for sensory input, The part of h...

The part of human brain which is a significant relay station for the sensory impulses and also is the origin of many of the involuntary acts of eye such as narrowing of the pupil i

Jaundice (icterus), Jaundice (Icterus) Jaundice is classified as pre-he...

Jaundice (Icterus) Jaundice is classified as pre-hepatic (hemolytic), hepatic and post-hepatic (obstructive) depending on origin of the problem, and is characterized by yellowi

Phylem porifera, please give us this phylem''s example...

please give us this phylem''s example...

Define the recommended dietary allowance of vitamin e, Define the Recommend...

Define the Recommended Dietary Allowance of Vitamin E? The requirements for the vitamin E are expressed in terms of tocopherol equivalents (TE) as mentioned earlier - 8 mg fo

Explain the rapport building, Q. Explain the Rapport Building? Maintain...

Q. Explain the Rapport Building? Maintaining good relationship with the client is very important in counselling. This will help you to gain trust, confidence of the client and

test the hypothesis , A classic study of extrasensory perception tests the...

A classic study of extrasensory perception tests the ability of one person to read another's mind. The test uses five cards with different designs, all known to both participants.

Define safety points that should used in the laboratory, Define Safety poin...

Define Safety points that should used in the Laboratory? You should be aware of the rules/regulations and the procedures before undertaking any practical work in the laboratory

Maintenance of the normal quantity of chromosomes, Q. Why is meiosis signif...

Q. Why is meiosis significant for the maintenance of the normal quantity of chromosomes of a species with sexual reproduction? A reduction to a half of the maximum normal quant

Which equipments are required for stress laboratory, The stress lab must ha...

The stress lab must have the following equipment: 1) A mercury Blood Pressure apparatus. 2) A Bed so that the patients ECG can be recorded in the recovery period in supine po

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd