Autoradiography, Biology

Assignment Help:

Autoradiography

Autoradiography is a modification of the radioactive tracer technique. In this technique, the radioactivity is detected by a thin film of photographic emulsion containing silver compounds which is put over a labelled cell or tissue. The rays emitted by radioisotope from the cell or tissue expose the photographic emulsion like a photographic film which is developed in a dark room. Silver grains are seen as black dots on parts of the specimen where radioisotope is accumulated.

Autoradiography reveals the metabolic processes of the cell. For example, by labelling cells with 3H thymidine, it is found that silver grains are localised in nucleus, which shows that DNA is synthesised in the nucleus. Similarly, by labelling cells with 3H uridine, silver grains are first seen in nucleus and then after some time these grains appear in the cytoplasm. This clearly demonstrates that RNA is initially synthesised in the nucleus but later on migrates to the cytoplasm.

Different radioisotopes are used for localising different macromolecules. As stated earlier,  cells are labelled with 3H thymidine for the studX of DNA metabolism, 3H uridine to study RNA metabolism, tritiated amino acids for examining the synthesis of proteins, treated monosaccharides such as 3H-mannose and 3H-fucose for examining the synthesis of polysaccharides.


Related Discussions:- Autoradiography

Explain lipolysis, Lipolysis What do we mean by lipolysis? Hydrolysis o...

Lipolysis What do we mean by lipolysis? Hydrolysis of ester bonds in lipids is called lipolysis. This may occur by enzyme action or by heat and moisture, resulting in the liber

Respiration , why should breathe faster after a hard work

why should breathe faster after a hard work

Apical window, The transducer is placed in the mid axillary line with ...

The transducer is placed in the mid axillary line with the transducer ridge pointing towards the patients left to obtain the four-chamber view. The left ventricular apex is

Kingdom animalia, in what order did the shellfish belong? in what genus did...

in what order did the shellfish belong? in what genus did the shellfish belong? in what species did the shellfish belong?

Describe in brief about failing implant, Describe in brief about failing im...

Describe in brief about failing implant The failing implant may show evidence of pocketing, bleeding upon probing, purulence, and indicates the bone loss patterns are progressi

Nutrient cycles, Nutrient Cycles All ecosystems have certain common ba...

Nutrient Cycles All ecosystems have certain common basic features of structure and function. They all have living and non-living components through which there is a flow of en

Human diseases caused by virus, Q. What are some human diseases caused by v...

Q. What are some human diseases caused by virus and what are their respective modes of transmission? The major viral diseases transmitted by respiratory secretions (cough, snee

Explain the occurrence of vitamin B1, Explain the Occurrence of Vitamin B 1...

Explain the Occurrence of Vitamin B 1 Vitamin B 1   occurs widely in the vegetable  kingdom. The richest sources for vitamin B 1 , as you may already know, are yeast, grain ge

What is the important condition for a protein, Q. What is the important con...

Q. What is the important condition for a protein to be identical to another protein? For a protein to be identical to another protein it is necessary for the sequence of amino

Explain isozymes, ISOZYMES Sometimes an  enzyme present  in  the  same ...

ISOZYMES Sometimes an  enzyme present  in  the  same  organism is  found  to  have different molecular forms but  catalyzing the  same  reaction. These are called  isozymes. Th

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd