Autoradiography, Biology

Assignment Help:

Autoradiography

Autoradiography is a modification of the radioactive tracer technique. In this technique, the radioactivity is detected by a thin film of photographic emulsion containing silver compounds which is put over a labelled cell or tissue. The rays emitted by radioisotope from the cell or tissue expose the photographic emulsion like a photographic film which is developed in a dark room. Silver grains are seen as black dots on parts of the specimen where radioisotope is accumulated.

Autoradiography reveals the metabolic processes of the cell. For example, by labelling cells with 3H thymidine, it is found that silver grains are localised in nucleus, which shows that DNA is synthesised in the nucleus. Similarly, by labelling cells with 3H uridine, silver grains are first seen in nucleus and then after some time these grains appear in the cytoplasm. This clearly demonstrates that RNA is initially synthesised in the nucleus but later on migrates to the cytoplasm.

Different radioisotopes are used for localising different macromolecules. As stated earlier,  cells are labelled with 3H thymidine for the studX of DNA metabolism, 3H uridine to study RNA metabolism, tritiated amino acids for examining the synthesis of proteins, treated monosaccharides such as 3H-mannose and 3H-fucose for examining the synthesis of polysaccharides.


Related Discussions:- Autoradiography

Predation, Think back to the patterns of predator-prey cycling from Section...

Think back to the patterns of predator-prey cycling from Sections 2 and 3. Which of the three predator functional responses would you expect to be most likely to give stable popula

Characteristics of ammonium transport system, Characteristics of Ammonium T...

Characteristics of Ammonium Transport System The following are the characteristics of ammonium transport system: Ammonium transport system is ammonia' repressible

Prevalence of coronaru heart diseases, Q. Prevalence of coronaru heart dise...

Q. Prevalence of coronaru heart diseases? Incidence of obesity, diabetes and CHD is increasing in India in almost all age groups. At present, obesity, which is a major risk fa

Fluid management in diarrhoea, Q. Fluid management in Diarrhoea? Fluid ...

Q. Fluid management in Diarrhoea? Fluid management: The key to diarrhoea management is the early replacement of fluid lost in the stools through intravenous or oral route. Whil

What is the nature of the stimulus received, What is the nature of the stim...

What is the nature of the stimulus received and transmitted by the neurons? Neurons receive and transmit chemical stimuli by neurotransmitters released in the synapses. Along

Agriculture, what''s a mature female sheep called

what''s a mature female sheep called

Mutual supplementation of protein with legume and milk, Define Mutual suppl...

Define Mutual supplementation of Protein with legume and milk? Improvement of cereal diets with legume and milk proteins: The proteins of poor diets based on different cereals

What is the significance of immunogenicity, Problem: Explain the proced...

Problem: Explain the procedure for import of cosmetics in US. - Briefly explain the procedure for import of cosmetics in US What are the major points to be considered whe

Photosynthesis rate varies according to the photic energy, Photosynthesis r...

Photosynthesis rate varies according to the photic energy intensity. Do the similar occur in aerobic respiration? What happens to the glucose balance as a result of these variation

Illustrate about the ward halstead and luria, Illustrate about the Ward Hal...

Illustrate about the Ward Halstead and Luria The linear approach is best exemplified in the work of A.R. Luria and various collaborators, while the configurational approach is

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd