Autoradiography, Biology

Assignment Help:

Autoradiography

Autoradiography is a modification of the radioactive tracer technique. In this technique, the radioactivity is detected by a thin film of photographic emulsion containing silver compounds which is put over a labelled cell or tissue. The rays emitted by radioisotope from the cell or tissue expose the photographic emulsion like a photographic film which is developed in a dark room. Silver grains are seen as black dots on parts of the specimen where radioisotope is accumulated.

Autoradiography reveals the metabolic processes of the cell. For example, by labelling cells with 3H thymidine, it is found that silver grains are localised in nucleus, which shows that DNA is synthesised in the nucleus. Similarly, by labelling cells with 3H uridine, silver grains are first seen in nucleus and then after some time these grains appear in the cytoplasm. This clearly demonstrates that RNA is initially synthesised in the nucleus but later on migrates to the cytoplasm.

Different radioisotopes are used for localising different macromolecules. As stated earlier,  cells are labelled with 3H thymidine for the studX of DNA metabolism, 3H uridine to study RNA metabolism, tritiated amino acids for examining the synthesis of proteins, treated monosaccharides such as 3H-mannose and 3H-fucose for examining the synthesis of polysaccharides.


Related Discussions:- Autoradiography

Procedures for diagnosis - demonstration of specific antigen, Demonstration...

Demonstration of specific antigen(s): The presence of the viral antigen in the infected tissues and cells can be demonstrated by fluorescent antibody technique (FAT) and

Mycroboilogy mycoplasma, How we write assimgment about mycobiology of mycop...

How we write assimgment about mycobiology of mycoplasma in detail

Explain about genetic engineering, Genetic engineering has been successfull...

Genetic engineering has been successfully used for producing: 1. Transgenic mice for testing safety of polio vaccine before use in humans 2. transgenic models for studying ne

Anticoagulant therapy, Anticoagulant therapy To prevent blood clottin...

Anticoagulant therapy To prevent blood clotting on the valve, patients with prosthetic valves require life long anticoagulant therapy. The patient is put on oral antico

Protein electrophoresis - measuring genetic diversity, Q. Protein electroph...

Q. Protein electrophoresis - Measuring Genetic Diversity? This method is now being used to measure genetic diversity. Genes contain the information required to produce enzymes

Potential air pollutants encountered in working environment, Question: ...

Question: a) Why is it important to maintain good standards of Indoor Air Quality in modern offices. Briefly explain the potential air pollutants encountered in such working

Explain interactions of macrominerals with other nutrients, Explain Interac...

Explain Interactions of Macrominerals With Other Nutrients? Various nutrients interact with minerals thereby affecting their bioavailability. These interactions occur at differ

Plasma proteins - circulation, Plasma Proteins - Circulation The total...

Plasma Proteins - Circulation The total protein concentration of the plasma is around 7% only. The plasma proteins are of three types - serum albumin (4%), serum globulin (2.7

Multiple alleles, MULTIPLE ALLELES (i)         More than two alternativ...

MULTIPLE ALLELES (i)         More than two alternative forms (alleles) of a gene in a population occupying the same locus on a chromosome or its homologue are known as multiple

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd