Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Autoradiography
Autoradiography is a modification of the radioactive tracer technique. In this technique, the radioactivity is detected by a thin film of photographic emulsion containing silver compounds which is put over a labelled cell or tissue. The rays emitted by radioisotope from the cell or tissue expose the photographic emulsion like a photographic film which is developed in a dark room. Silver grains are seen as black dots on parts of the specimen where radioisotope is accumulated.
Autoradiography reveals the metabolic processes of the cell. For example, by labelling cells with 3H thymidine, it is found that silver grains are localised in nucleus, which shows that DNA is synthesised in the nucleus. Similarly, by labelling cells with 3H uridine, silver grains are first seen in nucleus and then after some time these grains appear in the cytoplasm. This clearly demonstrates that RNA is initially synthesised in the nucleus but later on migrates to the cytoplasm.Different radioisotopes are used for localising different macromolecules. As stated earlier, cells are labelled with 3H thymidine for the studX of DNA metabolism, 3H uridine to study RNA metabolism, tritiated amino acids for examining the synthesis of proteins, treated monosaccharides such as 3H-mannose and 3H-fucose for examining the synthesis of polysaccharides.
General characters of phylum protozoa
Q. What is Regional wall motion abnormality? Ans. A critical occlusion of a coronary artery leads to myocardial ischemia which is manifested as regional wall motion abnorm
Reversible airways obstructive disease, advanced heart block, or episodic decompensation. Vasodilators Agents that dilate arteriolar smooth muscle and lower peripheral vascu
Suppose the following base sequence was found in a 21 base DNA polymer. 3' TAC TTA GGC TCC GTT CAG ATT 5' 1. Provide the nucleotide sequence complementary
The types of foods involved in botulism vary according to food preservation and eating habits in different regions. Any food that is conducive to outgrowth and toxin production, th
Sex Determination - Reproduction Sex, whether an individual will be a male or a female is determined at fertilisation, and this directs and controls all the later processes i
Glyceraldehyde has a one asymmetric carbon atom the central one and so two stereoisomers which is also known as optical isomers are possible, which is two forms of glyceraldehyde,
A 19 year old model for Glamour magazine tripped over an extension cord causing injury to her epidermis. She is afraid the injury will leave a scar. Based on your knowledge of rege
What is cold-shortening It is important to point out that over-effective chilling of hot carcasses can lead to toughness. If the temperature of the meat (muscles) can be reduce
How sugar play a major role in Fermentation? The production of chemicals by fermenting several sugars is a very well-accepted science. Its utilize ranges from producing beverag
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd