Autoradiography, Biology

Assignment Help:

Autoradiography

Autoradiography is a modification of the radioactive tracer technique. In this technique, the radioactivity is detected by a thin film of photographic emulsion containing silver compounds which is put over a labelled cell or tissue. The rays emitted by radioisotope from the cell or tissue expose the photographic emulsion like a photographic film which is developed in a dark room. Silver grains are seen as black dots on parts of the specimen where radioisotope is accumulated.

Autoradiography reveals the metabolic processes of the cell. For example, by labelling cells with 3H thymidine, it is found that silver grains are localised in nucleus, which shows that DNA is synthesised in the nucleus. Similarly, by labelling cells with 3H uridine, silver grains are first seen in nucleus and then after some time these grains appear in the cytoplasm. This clearly demonstrates that RNA is initially synthesised in the nucleus but later on migrates to the cytoplasm.

Different radioisotopes are used for localising different macromolecules. As stated earlier,  cells are labelled with 3H thymidine for the studX of DNA metabolism, 3H uridine to study RNA metabolism, tritiated amino acids for examining the synthesis of proteins, treated monosaccharides such as 3H-mannose and 3H-fucose for examining the synthesis of polysaccharides.


Related Discussions:- Autoradiography

Determine pull-ups test for check the body strength, Determine Pull-ups tes...

Determine Pull-ups test for check the body strength? This test is also done to measure upper body strength or endurance by maximum number of pull-ups completed. Subject hangs f

Define osseointegration and its theories, Q. Define osseointegration and it...

Q. Define osseointegration and its theories? Osseointegration implies that "it is a contact established without interposition of non bony tissue between normal remodeled bone a

Determine procedure for determination of protein content, Determine Procedu...

Determine Procedure for determination of protein content? Now carry out the procedure as enumerated herewith: 1. Preparation of standard albumin: A standard solution of 2 mg

Corelation of st depressoin with coronary angiography, Q. Corelation of ST ...

Q. Corelation of ST Depressoin with Coronary Angiography? Various investigators considered 1 mm of horizontal or downsloping ST depression to denote a positive test and used fr

Impulse transmission, The conduction of impulse in a nerve fibre is. a elec...

The conduction of impulse in a nerve fibre is. a electrochemical process. Maximum speed of transmission of nerve impulse can be 13 metre/second due to axoplasm is much resistant to

What is carbon cycle explain briefly, What is Carbon Cycle explain briefly?...

What is Carbon Cycle explain briefly? Life on earth is mainly carbon-based. Most of the material substances that make up living organisms consist of organic compounds of carbon

Explain about the dna viruses - carcinogenic, Explain about the DNA viruses...

Explain about the DNA viruses - Carcinogenic? The three DNA viruses found to cause human cancers are EBV, HBV and HPV. What are these? Let us find out. Epstein-Barr viru

Biochemistry, help me in writing assignment on racemization,mutarotation.

help me in writing assignment on racemization,mutarotation.

Theory of embryology - gradient theory, GRADIEN T THEORY - It was give...

GRADIEN T THEORY - It was given by T. Boveri & was supported by M.C. Child. Child called it as metabolic axial gradient theory. According to it, the egg has distinct ani

Phases of ovarian cycle, Phases of Ovarian Cycle In non-mammalian vert...

Phases of Ovarian Cycle In non-mammalian vertebrates the ovarian cycle consists of the following phase; Pre breeding phase Breeding phase and Post breedin

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd