Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Autoradiography
Autoradiography is a modification of the radioactive tracer technique. In this technique, the radioactivity is detected by a thin film of photographic emulsion containing silver compounds which is put over a labelled cell or tissue. The rays emitted by radioisotope from the cell or tissue expose the photographic emulsion like a photographic film which is developed in a dark room. Silver grains are seen as black dots on parts of the specimen where radioisotope is accumulated.
Autoradiography reveals the metabolic processes of the cell. For example, by labelling cells with 3H thymidine, it is found that silver grains are localised in nucleus, which shows that DNA is synthesised in the nucleus. Similarly, by labelling cells with 3H uridine, silver grains are first seen in nucleus and then after some time these grains appear in the cytoplasm. This clearly demonstrates that RNA is initially synthesised in the nucleus but later on migrates to the cytoplasm.Different radioisotopes are used for localising different macromolecules. As stated earlier, cells are labelled with 3H thymidine for the studX of DNA metabolism, 3H uridine to study RNA metabolism, tritiated amino acids for examining the synthesis of proteins, treated monosaccharides such as 3H-mannose and 3H-fucose for examining the synthesis of polysaccharides.
Name the Associated Foods used in bacillus cereus Cereal dishes that contain corn and corn starch, mashed potatoes, vegetables, minced meat, liver sausage, milk, cooked meat.
Explain Some food applications of pectin As with other viscous polyanions such as carrageenan, pectin may be protective towards milk casein colloids, enhancing the properties (
Define prevention of idd by Iodized Oil? The other approach employed as a specific measure for women and children in hyper-endemic areas is injection of iodized oil. Intramuscu
What is the difference between essential and natural amino acids? Ans) Significant amino acids are those that the organism is not able to synthesize and that require to be inges
SUBERIN It is a lipid formed by esterification of phellonic acid or its derivative with glycerol. Suberin occurs in the walls of cork cells and endodermal cells. It makes
Congenital Heart Diseases Congenital heart diseases are classified in many ways. One set of classification is cyanotic and acynotic conditions. Morphological classification
1. You have two bags of polymer. Bag A has 10 kgs of polymer with weight average molecular weight of 336.6 kg and Bag B has 20kg of polymer with weight average molecular weight 39
Descriibe Mechanical Ventilation and Cardiac Performance During spontaneous breathing, the intrathoracic and intrapulmonary pressures are negative, while with mechanical venti
Explain process to Control of Breathing? The rate of breathing is controlled by the medulla, the segment of brain stem just above the spinal cord. If the brain stem is cut just
Define Gastrointestinal tract - Excretion of Zinc in Humans? Majority of zinc is lost from the body in faeces. Endogenous zinc in the form of enzymes or metallo-proteins is sec
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd