Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Autoradiography
Autoradiography is a modification of the radioactive tracer technique. In this technique, the radioactivity is detected by a thin film of photographic emulsion containing silver compounds which is put over a labelled cell or tissue. The rays emitted by radioisotope from the cell or tissue expose the photographic emulsion like a photographic film which is developed in a dark room. Silver grains are seen as black dots on parts of the specimen where radioisotope is accumulated.
Autoradiography reveals the metabolic processes of the cell. For example, by labelling cells with 3H thymidine, it is found that silver grains are localised in nucleus, which shows that DNA is synthesised in the nucleus. Similarly, by labelling cells with 3H uridine, silver grains are first seen in nucleus and then after some time these grains appear in the cytoplasm. This clearly demonstrates that RNA is initially synthesised in the nucleus but later on migrates to the cytoplasm.Different radioisotopes are used for localising different macromolecules. As stated earlier, cells are labelled with 3H thymidine for the studX of DNA metabolism, 3H uridine to study RNA metabolism, tritiated amino acids for examining the synthesis of proteins, treated monosaccharides such as 3H-mannose and 3H-fucose for examining the synthesis of polysaccharides.
Normal 0 false false false EN-IN X-NONE X-NONE MicrosoftInternetExplorer4
Agar Agar is a polysaccharide found in the cell walls of some red algae and is unusual in containing sulfated galactose monomers. Certain marine algae of the class Rhodophyceae
It is important to recognise underlying causes and precipitating factors of heart failure for its appropriate management. That would also help in prevention and treatment of heart
Q. Classification and Justification of hemichordata? Kingdom Animalia Animals; multicellular organisms with cells that lack a cell wall, many capable of inovement or movement o
PADP may be determined by measuring the end diastolic velocity of PADP = 4 V 2 Pulmonary regurgitant signal and applying the following formula V is the end diastolic veloci
Development of Reserves - Conservation of Wildlife Establishment of Biological reserves, National parks, Forest reserves, Wildlife refuges and Biosphere reserves are effective
Q. Are proteins with the same number of each dissimilar amino acid that forms them necessarily identical proteins? Even if many proteins have the same number of each different
What is the excretory organ of an agama lizard
why does the monohybrid always result in a 1:2:1 ratio?
Name the state in humans in which the blood cholesterol content becomes abnormally high. a) Explain vernalisation. b) Many villagers near Industrial area vary from "blue bab
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd