Autoradiography, Biology

Assignment Help:

Autoradiography

Autoradiography is a modification of the radioactive tracer technique. In this technique, the radioactivity is detected by a thin film of photographic emulsion containing silver compounds which is put over a labelled cell or tissue. The rays emitted by radioisotope from the cell or tissue expose the photographic emulsion like a photographic film which is developed in a dark room. Silver grains are seen as black dots on parts of the specimen where radioisotope is accumulated.

Autoradiography reveals the metabolic processes of the cell. For example, by labelling cells with 3H thymidine, it is found that silver grains are localised in nucleus, which shows that DNA is synthesised in the nucleus. Similarly, by labelling cells with 3H uridine, silver grains are first seen in nucleus and then after some time these grains appear in the cytoplasm. This clearly demonstrates that RNA is initially synthesised in the nucleus but later on migrates to the cytoplasm.

Different radioisotopes are used for localising different macromolecules. As stated earlier,  cells are labelled with 3H thymidine for the studX of DNA metabolism, 3H uridine to study RNA metabolism, tritiated amino acids for examining the synthesis of proteins, treated monosaccharides such as 3H-mannose and 3H-fucose for examining the synthesis of polysaccharides.


Related Discussions:- Autoradiography

Define myocardial infraction and stress testing, Q. Define Myocardial infra...

Q. Define Myocardial infraction and stress testing? Prediction of disease is one of the primary functions of stress testing. We would like to be able to predict in each patient

Define the prevention of iron deficiency anaemia, Define the Prevention of ...

Define the Prevention of iron deficiency anaemia? As in the case of vitamin A deficiency, correction and prevention of dietary inadequacy of iron are important sustainable meth

Production of healthcare - contestability and measurability, Normal 0 ...

Normal 0 false false false EN-IN X-NONE X-NONE MicrosoftInternetExplorer4

Others factors affecting occupational health, Others Factors Affecting Occu...

Others Factors Affecting Occupational Health The list of factors which affect the occupational health and that need standard organising, scrutiny, inspection, surveillance and

Protection of protein meals or bypass protein, Protection of protein meals ...

Protection of protein meals or bypass protein The protein of oil cakes, having higher protein degradability, such as groundnut cake, mustard cake are utilized in the rumen les

The regulation mechanism of glycogenolysis, The  regulation mechanism invo...

The  regulation mechanism involves: a)  The hormones catecholamines (epinephrine, norepinephrine) and glucagon cause the increase in CAMP levels in cells. This CAMP activates p

Pocket psedocyst-endodontics principles and practice, Pocket Psedocyst : le...

Pocket Psedocyst : lesions present as endodontic origins from canals . It makes lumens resemble granuloma . All lesions lined until root canal or apical foramen communicat

Define the bioavailability of cyanocobalamin, Define the Bioavailability of...

Define the Bioavailability of cyanocobalamin? Vitamin B 12 is widely available. Availability is more from non-vegetarian foods as described earlier under the food sources sec

Dna primer, what is the function of dna primer

what is the function of dna primer

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd