Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Autoradiography
Autoradiography is a modification of the radioactive tracer technique. In this technique, the radioactivity is detected by a thin film of photographic emulsion containing silver compounds which is put over a labelled cell or tissue. The rays emitted by radioisotope from the cell or tissue expose the photographic emulsion like a photographic film which is developed in a dark room. Silver grains are seen as black dots on parts of the specimen where radioisotope is accumulated.
Autoradiography reveals the metabolic processes of the cell. For example, by labelling cells with 3H thymidine, it is found that silver grains are localised in nucleus, which shows that DNA is synthesised in the nucleus. Similarly, by labelling cells with 3H uridine, silver grains are first seen in nucleus and then after some time these grains appear in the cytoplasm. This clearly demonstrates that RNA is initially synthesised in the nucleus but later on migrates to the cytoplasm.Different radioisotopes are used for localising different macromolecules. As stated earlier, cells are labelled with 3H thymidine for the studX of DNA metabolism, 3H uridine to study RNA metabolism, tritiated amino acids for examining the synthesis of proteins, treated monosaccharides such as 3H-mannose and 3H-fucose for examining the synthesis of polysaccharides.
When the syndrome sets in at a rapid rate before the compensatory mechanisms become operative, acute heart failure develops. The examples are acute heart failure due to acute myoca
Which of the below determines the major difference between bryophytes (pron: bry-oh-fites) and tracheophytes (pron: tray-key-o-fites)? a) Tracheophytes can make their own food
We have no antibodies against any blood group when we are born still we say that some blood groups are incompatible for us.why?
Hydrogen ( h) has 1 electron; Carbon ( c) has 6 electrons, use the octet rule to determine which of the following is most stable. Note: some of these may have double bonds. A. O
Prevention and Control of Shigella food borne infection The control of Shigella food borne infection is similar to that of salmonellae; avoiding contamination of foods by ani
What are the differences between genomes in simple eukaryotes vs complex eukaryotes?
These views are obtained by placing the transducer in the suprasternal notch with the ridge pointing cephalad. The neck is extended during the suprasternal notch examination. Thi
Syngamy The pollen tube grows to a very limited extent in the synergid. It releases the contents either through a terminal or a subterminal pore. The contents include the two
Anaphase This phase is of shortest duration. It begins with a sudden separation of the two chromatids of each due to splitting of its centromere and then a slow mov
What are X-linked traits? The X-linked traits are the phenotypical traits conditioned by genes located in the nonhomologous (heterologous) portions of the X chromosome.
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd