Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Autoradiography
Autoradiography is a modification of the radioactive tracer technique. In this technique, the radioactivity is detected by a thin film of photographic emulsion containing silver compounds which is put over a labelled cell or tissue. The rays emitted by radioisotope from the cell or tissue expose the photographic emulsion like a photographic film which is developed in a dark room. Silver grains are seen as black dots on parts of the specimen where radioisotope is accumulated.
Autoradiography reveals the metabolic processes of the cell. For example, by labelling cells with 3H thymidine, it is found that silver grains are localised in nucleus, which shows that DNA is synthesised in the nucleus. Similarly, by labelling cells with 3H uridine, silver grains are first seen in nucleus and then after some time these grains appear in the cytoplasm. This clearly demonstrates that RNA is initially synthesised in the nucleus but later on migrates to the cytoplasm.Different radioisotopes are used for localising different macromolecules. As stated earlier, cells are labelled with 3H thymidine for the studX of DNA metabolism, 3H uridine to study RNA metabolism, tritiated amino acids for examining the synthesis of proteins, treated monosaccharides such as 3H-mannose and 3H-fucose for examining the synthesis of polysaccharides.
Vitamin a deficiency leads to a variety of disorders of the eyes and this effects the vision. Some of the disorders due to vitamin A deficiency are given below. 1) Night Blindne
Explain about the Thiamin - Vitamin B or aneurin? Thiamin is one of the earliest recognized vitamins. The chemical structure of thiamine was established by Williams in 1936. Th
Amino acid is any of a class of 20 molecules which are combined to form the proteins in living things. Consisting of the general formula NH2-CHR-COOH, where "R" is the side chain
Define the word colloid The word colloid, you may be interested to know, is derived from the Greek word "kolla" meaning "glue" and is defined as a system containing particles o
Q. Enumerate the various physical agents of sterilization, and list the advantages of moist heat in sterilization process Various physical agents of sterilization are Sun
how pollination occurs in Salvia?
Q. What are the chemical bonds of the DNA molecule that are broken for replication process to occur? During the DNA replication process, hydrogen bonds between the nitrogen- co
Which of the below are skin receptors in humans which are sensitive to heat? Are they: a) End organs of Krause b) Meissner's corpuscles c) End organs of Ruffini d)
how many grams of table sugar would you need to dissolved in water to produce 0.75 liters of a 0:25 molar acqeous solution of table sugar?
The overall goal of BCC programs for diabetes mellitus is to promote behaviors that control diabetes mellitus and prevent complications. These include: Following treatment
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd