Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Autoradiography
Autoradiography is a modification of the radioactive tracer technique. In this technique, the radioactivity is detected by a thin film of photographic emulsion containing silver compounds which is put over a labelled cell or tissue. The rays emitted by radioisotope from the cell or tissue expose the photographic emulsion like a photographic film which is developed in a dark room. Silver grains are seen as black dots on parts of the specimen where radioisotope is accumulated.
Autoradiography reveals the metabolic processes of the cell. For example, by labelling cells with 3H thymidine, it is found that silver grains are localised in nucleus, which shows that DNA is synthesised in the nucleus. Similarly, by labelling cells with 3H uridine, silver grains are first seen in nucleus and then after some time these grains appear in the cytoplasm. This clearly demonstrates that RNA is initially synthesised in the nucleus but later on migrates to the cytoplasm.Different radioisotopes are used for localising different macromolecules. As stated earlier, cells are labelled with 3H thymidine for the studX of DNA metabolism, 3H uridine to study RNA metabolism, tritiated amino acids for examining the synthesis of proteins, treated monosaccharides such as 3H-mannose and 3H-fucose for examining the synthesis of polysaccharides.
Define Disadvantages of using yeast as a source of protein? 1. Less protein yield (45-60%) 2. Growth rate is low (1-3 h) 3. High nucleic acid content leading to the forma
State the term - Halsted Reitan varies with the particular test Scoring for the Halsted Reitan varies with the particular test, such that individual scores may be expressed in
PENIS - Intromittent organ. Erectile, its apex is penis glanse. Covered by prepuce (part of skin). Main function is insemination (To transfer sperms in vagina in deep).
Non-Conventional Feed Resources There are many non-traditional or non-conventional materials which are available in abundance and can form potential source of feedstuffs for f
Practical Points in Preparation of Nursing Care Plan You first review the nursing diagnosis, then assign this priority or the order in which these are to be met. Alo
Ask qimporatance of sponge in tourism industry
How is the development of JACK FUIT fruit takes place from seed to fruit?
in what aspect are the cnidarians similar to protozoans? to the poriferans?
Q. What are the five classes of vertebrates? To which of these do human beings belong? The five classes of vertebrates are amphibians, reptiles, fishes (osteichthyes and chondr
Ask question which soil is good
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd