Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Autoradiography
Autoradiography is a modification of the radioactive tracer technique. In this technique, the radioactivity is detected by a thin film of photographic emulsion containing silver compounds which is put over a labelled cell or tissue. The rays emitted by radioisotope from the cell or tissue expose the photographic emulsion like a photographic film which is developed in a dark room. Silver grains are seen as black dots on parts of the specimen where radioisotope is accumulated.
Autoradiography reveals the metabolic processes of the cell. For example, by labelling cells with 3H thymidine, it is found that silver grains are localised in nucleus, which shows that DNA is synthesised in the nucleus. Similarly, by labelling cells with 3H uridine, silver grains are first seen in nucleus and then after some time these grains appear in the cytoplasm. This clearly demonstrates that RNA is initially synthesised in the nucleus but later on migrates to the cytoplasm.Different radioisotopes are used for localising different macromolecules. As stated earlier, cells are labelled with 3H thymidine for the studX of DNA metabolism, 3H uridine to study RNA metabolism, tritiated amino acids for examining the synthesis of proteins, treated monosaccharides such as 3H-mannose and 3H-fucose for examining the synthesis of polysaccharides.
Fensom and Spanner Electroosmotic Flow Hypothesis In electroosmosis the ions flow across a membrane in response to electrical gradient. Ions pull along water and other content
Dfine Digestion, Absorption and Transport of proteins? Under this sub-section, we shall learn how proteins that we consume are digested, absorbed and transported to various bod
Q. What is the ADP phosphorylation? What respectively are oxidative phosphorylation and photophosphorylation? ADP phosphorylation is the addition of one inorganic phosphate in
What is the main difference between a bird heart and a mammalian heart? The bird heart has a one aortic arch on the right side of its body while the mammalian heart has one on
Plasmid is the class of the circular, extrachromosomal, autonomously replicating, DNA elements found in number of bacteria. Contain origins of the replication to ensure their main
what are the origin, morphology, active constituents, uses and market preparation of tanco beans ?
State about polyploid cells During development cells of certain tissues like the salivary gland and liver produce extraordinary amounts of selected proteins. This is often achi
How does aggregation occur in sickle-cell anemia? Place the steps in the correct order from no aggregation to sickle red blood cell. Note that deoxyhemoglobin is generally in the T
Tumor Suppressor Genes Tumor suppressor genes or anti-oncogenes generally inhibit cell division in cooperation with proto-oncogenes. The very existence of these genes prevent
Explain the Nutrition of Fungi? Fungi grow in moist environment and are chemoorganoheterotrophs. What do we mean by chemoorganoheterotrophs? The term chemotroph describes organ
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd