Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Autoradiography
Autoradiography is a modification of the radioactive tracer technique. In this technique, the radioactivity is detected by a thin film of photographic emulsion containing silver compounds which is put over a labelled cell or tissue. The rays emitted by radioisotope from the cell or tissue expose the photographic emulsion like a photographic film which is developed in a dark room. Silver grains are seen as black dots on parts of the specimen where radioisotope is accumulated.
Autoradiography reveals the metabolic processes of the cell. For example, by labelling cells with 3H thymidine, it is found that silver grains are localised in nucleus, which shows that DNA is synthesised in the nucleus. Similarly, by labelling cells with 3H uridine, silver grains are first seen in nucleus and then after some time these grains appear in the cytoplasm. This clearly demonstrates that RNA is initially synthesised in the nucleus but later on migrates to the cytoplasm.Different radioisotopes are used for localising different macromolecules. As stated earlier, cells are labelled with 3H thymidine for the studX of DNA metabolism, 3H uridine to study RNA metabolism, tritiated amino acids for examining the synthesis of proteins, treated monosaccharides such as 3H-mannose and 3H-fucose for examining the synthesis of polysaccharides.
Explain about the Dietary Reference Intakes? Dietary Reference Intakes (DRIs) are relatively new to the field of nutrition. The DRIs are a set of four nutrient-based reference
Bone Physiology and Wound Healing The events involved in osseous wound healing around implants recapitulate the events of wound healing which is depicted in the flowchart.
Manual and Automatic Toothbrushes Brushing is imperative and soft or medium toothbrushes are recommended. Small heads are useful because they allow better access. Automatic too
Consequences of Air Pollution The physiological effects of toxic pollutants on living beings can be of two types: Acute effects and Chronic effects. Acute effec
what are the modes of nutrition in different animals
Fibroblast is the term applied to a cell of connective tissue which is separated from similar cells by some degree of the matrix material; fibroblasts secrete elastin and collagen
ANIMALS THAT ARE PROTOZOA????
Q. What do you mean by Borrowed Materials? For the study of as much relevant material as possible the material is obtained on loan. Borrowed specimens benefit the' lending inst
ESR and CRP are elevated in almost all patients of arthritis and carditis and rarely in patients with chorea. ESR should be repeated periodically as it is useful in following the c
Objectives of psychological components It is essential to have objective scores, in order to identify and classify deficits in psychological components and processes. The nee
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd