Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Autoradiography
Autoradiography is a modification of the radioactive tracer technique. In this technique, the radioactivity is detected by a thin film of photographic emulsion containing silver compounds which is put over a labelled cell or tissue. The rays emitted by radioisotope from the cell or tissue expose the photographic emulsion like a photographic film which is developed in a dark room. Silver grains are seen as black dots on parts of the specimen where radioisotope is accumulated.
Autoradiography reveals the metabolic processes of the cell. For example, by labelling cells with 3H thymidine, it is found that silver grains are localised in nucleus, which shows that DNA is synthesised in the nucleus. Similarly, by labelling cells with 3H uridine, silver grains are first seen in nucleus and then after some time these grains appear in the cytoplasm. This clearly demonstrates that RNA is initially synthesised in the nucleus but later on migrates to the cytoplasm.Different radioisotopes are used for localising different macromolecules. As stated earlier, cells are labelled with 3H thymidine for the studX of DNA metabolism, 3H uridine to study RNA metabolism, tritiated amino acids for examining the synthesis of proteins, treated monosaccharides such as 3H-mannose and 3H-fucose for examining the synthesis of polysaccharides.
How calcium prevents luxury consumption of potassium Increasing Ca:K ratio in soil solution depresses the uptake of potassium. Thus one of the harmful effects of over liming m
ENERG Y TRANSFORMATION - During photosynthesis radiant energy is converted into chemical energy by green plants. In biluminiscent organisms .e.g glow worm, noctilluca &
complete assignment
Explain the s1M filament theory of muscle contraction. What is special of "FlavrSavr" variety of tomato? Why is it preferred to its normal native variety? Illustrate a label
With the help of reaction briefly explain the oxidative phase of the pentose phosphate pathway. The oxidative branch of the pathway generates NADPH and pentose-5 phosphate, th
Signs and Symptoms Decreased urine output Bloody or brown colour urine Edema Malaise Mild headache Gastrointestinal disturbances
Patient after cardiac transplant The major focus of medical and nursing care after transplantation is to prevent early identification of rejection so that appropriate
Explain the OBJECTIVE of Cardiac Rehabilitation ? After going through this unit, you should be able to: 1 identifies the eligible candidate for cardiac rehabilitation and be
Preliminary treatment of implant therapy It is important to ensure a healthy oral environment before going ahead with implant procedure. The treatment needs to be initiated in
Dideoxy Sequencing is the enzymatic determination and consideration of DNA or RNA sequence by the rechnique of Sanger and colleagues, based on incorporation of the chain terminati
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd