Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Autoradiography
Autoradiography is a modification of the radioactive tracer technique. In this technique, the radioactivity is detected by a thin film of photographic emulsion containing silver compounds which is put over a labelled cell or tissue. The rays emitted by radioisotope from the cell or tissue expose the photographic emulsion like a photographic film which is developed in a dark room. Silver grains are seen as black dots on parts of the specimen where radioisotope is accumulated.
Autoradiography reveals the metabolic processes of the cell. For example, by labelling cells with 3H thymidine, it is found that silver grains are localised in nucleus, which shows that DNA is synthesised in the nucleus. Similarly, by labelling cells with 3H uridine, silver grains are first seen in nucleus and then after some time these grains appear in the cytoplasm. This clearly demonstrates that RNA is initially synthesised in the nucleus but later on migrates to the cytoplasm.Different radioisotopes are used for localising different macromolecules. As stated earlier, cells are labelled with 3H thymidine for the studX of DNA metabolism, 3H uridine to study RNA metabolism, tritiated amino acids for examining the synthesis of proteins, treated monosaccharides such as 3H-mannose and 3H-fucose for examining the synthesis of polysaccharides.
Q. What are the oligosaccharides, monosaccharides and polysaccharides? Monosaccharides are simple molecules of carbohydrates that cannot be broken down into smaller molecules o
In Drosophila, these genes occur on chromosome 4. H - wild type (normal) h - hairy F - wild type f - frizzled E - wild type e - vest
I WANT JOB FOR TEACHING SCINCE SUBJECT FOR ONLINE
Production of Rare Hybrids Hybridization is a well established plant breeding procedure to obtain superior plants by combining useful characters distributed in different plan
Counselling a diabetic patient and their family members is very important as patient has to modify the life style and diet and needs family support. Therefore it is important for y
Normal 0 false false false EN-IN X-NONE X-NONE MicrosoftInternetExplorer4
Explain Asystole and Pulseless Electrical Activity (PEA) The outcomes from these rhythms are generally much worse than for VT/VF, unless a readily correctable cause is identif
how do they adapt to their parasitic mode of feeding
Action of Hormones We said earlier that hormones are released into the blood stream or extracellular fluid and therefore, reach most of the cells of the body. However, they
Q. Which are the beings that form the kingdom Fungi? The kingdom Fungi is created by fungi. Q. Which are the beings that form the kingdom Plantae? Are algae parts of this k
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd