Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Autoradiography
Autoradiography is a modification of the radioactive tracer technique. In this technique, the radioactivity is detected by a thin film of photographic emulsion containing silver compounds which is put over a labelled cell or tissue. The rays emitted by radioisotope from the cell or tissue expose the photographic emulsion like a photographic film which is developed in a dark room. Silver grains are seen as black dots on parts of the specimen where radioisotope is accumulated.
Autoradiography reveals the metabolic processes of the cell. For example, by labelling cells with 3H thymidine, it is found that silver grains are localised in nucleus, which shows that DNA is synthesised in the nucleus. Similarly, by labelling cells with 3H uridine, silver grains are first seen in nucleus and then after some time these grains appear in the cytoplasm. This clearly demonstrates that RNA is initially synthesised in the nucleus but later on migrates to the cytoplasm.Different radioisotopes are used for localising different macromolecules. As stated earlier, cells are labelled with 3H thymidine for the studX of DNA metabolism, 3H uridine to study RNA metabolism, tritiated amino acids for examining the synthesis of proteins, treated monosaccharides such as 3H-mannose and 3H-fucose for examining the synthesis of polysaccharides.
State in brief about the Comprehensive neuropsychology Comprehensive neuropsychology testing needs a combination of Context related ideometric and quantitative psychometric app
True cyst: cyst that is completely lined by epithelial tissues.All the lesions are far away without communication with endodontic access or endodontic cavity or periapical
Is herbivorism a form of predatism? Herbivorism is a type of predatism in which first order consumers feed from producers (plants or algae). For instance, birds and fruits, hum
What are the functions of insulin and glucagon for the blood glucose control? Glucagon enhances glycemia and insulin reduces it. They are antagonistic pancreatic hormones. Gluc
How Drugs Increase Food Intake? You must understand that drugs may also increase food intake. This is because they can: Stimulate appetite : This is a common side effect
Physiological Changes - Consequences of Aging Various physiological regulatory mechanisms show decreased efficiency due to aging. For instance, normally the glucose level in t
Q. What do you mean by Maxilla? The maxilla has ample keratinized tissue that covers the entire palate. This tissue is well-attached and more difficult to mobilize. Nevertheles
Explain some Advantages of Most Probable Number? 1. It is simple and method of choice for determining fecal coliform densities. 2. Results of different laboratories are comp
The diet of a MSUD patient (child) should therefore involve: • Measured quantities of natural protein or leucine from foods. • A BCAA free protein, vitamin and mineral supp
Tick-Borne Encephalitis (TBE ) TBE occurs in Scandinavia, Western and Central Europe and countries of the former USSR, mainly in rural forested areas. Risk is greatest from Apri
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd