Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Autoradiography
Autoradiography is a modification of the radioactive tracer technique. In this technique, the radioactivity is detected by a thin film of photographic emulsion containing silver compounds which is put over a labelled cell or tissue. The rays emitted by radioisotope from the cell or tissue expose the photographic emulsion like a photographic film which is developed in a dark room. Silver grains are seen as black dots on parts of the specimen where radioisotope is accumulated.
Autoradiography reveals the metabolic processes of the cell. For example, by labelling cells with 3H thymidine, it is found that silver grains are localised in nucleus, which shows that DNA is synthesised in the nucleus. Similarly, by labelling cells with 3H uridine, silver grains are first seen in nucleus and then after some time these grains appear in the cytoplasm. This clearly demonstrates that RNA is initially synthesised in the nucleus but later on migrates to the cytoplasm.Different radioisotopes are used for localising different macromolecules. As stated earlier, cells are labelled with 3H thymidine for the studX of DNA metabolism, 3H uridine to study RNA metabolism, tritiated amino acids for examining the synthesis of proteins, treated monosaccharides such as 3H-mannose and 3H-fucose for examining the synthesis of polysaccharides.
Q. What are the vessels that carry blood to the kidneys? Is this blood venous or arterial? The renal arteries are ramifications of the aorta and so the blood filtered by the ki
what is ascus ?
Phylogenetic or Cladistic Classification Phylogeny plays a great role in classification. It is the appropriate theoretical background for taxonomy and is quite essential in exp
Pupillary reflexes There are two types of reflexes which control the pupillary reaction-the light reflex and near reflex. When light is shown on one eye, there is a constrictio
Light - Marine Ecosystems Light is a limiting factor in the ocean as it contributes significantly to organic production and distribution of marine life. The ocean is divisible
Polynephritis in cattle Contagious bovine polynephritis is a specific infection of urinary tract of cattle. The causative agent is Corynebacterium renale, a rod shaped gram-po
Xenobiotic compounds are man-made chemicals that are present in the environment at unnaturally high concentrations. The xenobiotic compounds are either not produced naturally, o
What is cyclosis? Cyclosis is a type of internal cell movement in which an oriented flow of circulating material is formed and maintained in the cytoplasm by the action of micr
Explain Syndrome X as a Effect of Obesity? People with intra-abdominal obesity with high waist- to- hip ratio are more prone to develop the metabolic syndrome X. This is charac
Abdomen check the contour of the abdomen; normally it should be soft, symmetrical, slightly round and moves in synchronously with chest in premature neonate abdomen w
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd