Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Autoradiography
Autoradiography is a modification of the radioactive tracer technique. In this technique, the radioactivity is detected by a thin film of photographic emulsion containing silver compounds which is put over a labelled cell or tissue. The rays emitted by radioisotope from the cell or tissue expose the photographic emulsion like a photographic film which is developed in a dark room. Silver grains are seen as black dots on parts of the specimen where radioisotope is accumulated.
Autoradiography reveals the metabolic processes of the cell. For example, by labelling cells with 3H thymidine, it is found that silver grains are localised in nucleus, which shows that DNA is synthesised in the nucleus. Similarly, by labelling cells with 3H uridine, silver grains are first seen in nucleus and then after some time these grains appear in the cytoplasm. This clearly demonstrates that RNA is initially synthesised in the nucleus but later on migrates to the cytoplasm.Different radioisotopes are used for localising different macromolecules. As stated earlier, cells are labelled with 3H thymidine for the studX of DNA metabolism, 3H uridine to study RNA metabolism, tritiated amino acids for examining the synthesis of proteins, treated monosaccharides such as 3H-mannose and 3H-fucose for examining the synthesis of polysaccharides.
what is the nature and scope of zoology?
Explain of Functional property Browning/Flavour/ Aroma Mode of action Proteins contribute to browning by reacting with lactose and other reducing sugars present in a form
What is the relationship of hydrogen ion concentration in tomato juice relative to lemon juice and to pure water? Be as quantitative as you can (i.e., use numbers to describe the m
Which vitamin is useful for the clotting of blood?
Determine about the degenerative diseases Immune responses in brain can quickly spiral out of control and cause further harm. In degenerative diseases such as Parkinson's, Alz
LEN S - In lumen of eye ball, a large colourless, transparent, more convex on back side, lens present covered by lens capsule. When white tissues are formed in it, it b
Q. Prevention strategy for diverticular disease? The prevention strategy for the disease involves the following: • Eat a high-fiber diet (more than 15 g/day of crude fiber)
Darwin while evolving the concept of natural selection laid emphasis on competition among individuals belonging to a species, that is intraspecific competition. Here the individual
Etiology The causes of diarrhoea can be classified as follows: i) Infectious causes Viral : Rota virus, Norwalk and allied viruses, Enterovirus, Influenza virus,
Determine the Adequacy of children's food? Adequacy of children's food and nutrient intake depends on: Sibling company Peer pressure Model set by parents and
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd