Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Autoradiography
Autoradiography is a modification of the radioactive tracer technique. In this technique, the radioactivity is detected by a thin film of photographic emulsion containing silver compounds which is put over a labelled cell or tissue. The rays emitted by radioisotope from the cell or tissue expose the photographic emulsion like a photographic film which is developed in a dark room. Silver grains are seen as black dots on parts of the specimen where radioisotope is accumulated.
Autoradiography reveals the metabolic processes of the cell. For example, by labelling cells with 3H thymidine, it is found that silver grains are localised in nucleus, which shows that DNA is synthesised in the nucleus. Similarly, by labelling cells with 3H uridine, silver grains are first seen in nucleus and then after some time these grains appear in the cytoplasm. This clearly demonstrates that RNA is initially synthesised in the nucleus but later on migrates to the cytoplasm.Different radioisotopes are used for localising different macromolecules. As stated earlier, cells are labelled with 3H thymidine for the studX of DNA metabolism, 3H uridine to study RNA metabolism, tritiated amino acids for examining the synthesis of proteins, treated monosaccharides such as 3H-mannose and 3H-fucose for examining the synthesis of polysaccharides.
In detail describe the structure and function of the heart and blood flow. Include the conduction system and blood vessels. Make your response specific to your audience who are a g
Explain about the Fat metabolism - Ageing? With increasing age, the blood cholesterol and blood triglyceride levels gradually increase. Certain factors like the kind and amoun
physical properties of protoplasm
Considering a pair of homologous chromosomes containing a gene having two different alleles how many different genotypes can the individual present? If a gene of the diploid sp
Aeolian Wind transported materials constitute this category. This can be further divided into dunes or loess. Dunes are found in thr& types of situations. Fitly, these may occu
detail about phylum protozoa?
DISEASE CONCERNED WITH KIDNEY - 1. Paget's disease - Abnormalities of kidney. 2. Uramia - Increases in quantity of urea. 3. Nephritis - Inflamation in glom
What is a Cell? The body of every living being is made up a living matter or bio matter. In the organisms these matters occur only in the form of minute membrane bound unit lum
Before stem cuttings are planted, the cut end of the stem is often dipped in a hormone powder. What is the point of this? The hormone powder having a plant growth substanc
Testicular Hypoplasia Hypoplasia refers to testicular disorders related to congenitally underdeveloped and underactive spermatic epithelium of one or both testes even though t
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd