Autoradiography, Biology

Assignment Help:

Autoradiography

Autoradiography is a modification of the radioactive tracer technique. In this technique, the radioactivity is detected by a thin film of photographic emulsion containing silver compounds which is put over a labelled cell or tissue. The rays emitted by radioisotope from the cell or tissue expose the photographic emulsion like a photographic film which is developed in a dark room. Silver grains are seen as black dots on parts of the specimen where radioisotope is accumulated.

Autoradiography reveals the metabolic processes of the cell. For example, by labelling cells with 3H thymidine, it is found that silver grains are localised in nucleus, which shows that DNA is synthesised in the nucleus. Similarly, by labelling cells with 3H uridine, silver grains are first seen in nucleus and then after some time these grains appear in the cytoplasm. This clearly demonstrates that RNA is initially synthesised in the nucleus but later on migrates to the cytoplasm.

Different radioisotopes are used for localising different macromolecules. As stated earlier,  cells are labelled with 3H thymidine for the studX of DNA metabolism, 3H uridine to study RNA metabolism, tritiated amino acids for examining the synthesis of proteins, treated monosaccharides such as 3H-mannose and 3H-fucose for examining the synthesis of polysaccharides.


Related Discussions:- Autoradiography

Define repletion studies for studying nutrient requirement, Explain the Dep...

Explain the Depletion and Repletion Studies for studying nutrient requirement? This is an experimental procedure in which volunteer subjects are kept on a diet devoid of a part

Explain national nutritional anaemia prophylaxis programme, Explain Nationa...

Explain National Nutritional Anaemia Prophylaxis Programme (NNAPP)? In past, both access to and intake of iron-folate tablets have been poor in children and therefore had a lit

Classification of science, Classification: There were many similariti...

Classification: There were many similarities between things or phenomena which led to their classification. The first classifications were in terms of beings (the living), th

Symmetry system characterized in echinoderms, Q. How are the nervous and th...

Q. How are the nervous and the symmetry system characterized in echinoderms? Adult echinoderms, along with cnidarians, are the animals that present radial symmetry that is thei

Meaning of behaviour change communication, Behaviour change communication i...

Behaviour change communication is necessary as it ensures that the diabetic patient and their families have access to correct information about the disease, complications, treatm

Which disease is associated with rapid dehydration, Which disease is associ...

Which disease is associated with the following symptoms? Sudden onset of profuse watery stool followed by vomiting, rapid dehydration and muscular cramps?

FEEDING MECHANISM., EXPLAIN IN DETAILS FEEDING MECHANISM IN MAN.

EXPLAIN IN DETAILS FEEDING MECHANISM IN MAN.

Define hydroxybenzoic acid and hydroxycinnamic acid, Define Hydroxybenzoic ...

Define Hydroxybenzoic acid and Hydroxycinnamic acid? Hydroxybenzoic acid: e.g. ellagic and gallic acids, which are hydrolyzable tannins, present in berries and nuts. Hydroxy

Skin cancer, SKIN CANCER: CAUSES AND TREATMENT PURPOSE: The objective of t...

SKIN CANCER: CAUSES AND TREATMENT PURPOSE: The objective of this assignment is to make you aware of the structure and function of your skin, what are the dangers that your skin ma

What is neurotransmitters , When the action potential reaches the nerve ter...

When the action potential reaches the nerve terminal it causes the let go of a chemical neurotransmitter by the synaptic vesicles. Mammalian nervous system employs many substances

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd