Automated dna sequencing, Biology

Assignment Help:

Automated DNA sequencing is now common place which is based on the chain termination method but using a fluorescent dye attached to an oligonucleotide primer instead of using radioactive labeling.  A several  fluorescent  dye is attached  to the primer  for every  of the four  sequencing  reactions  but,  follow  incubation,  all four combinations  are electrophoresed    and combined on one gel lane. An laser detection  systems  then  distinguish  the  self  of  every  termination  product  as  it elutes  from  the gel. The sequence in that the several fluorescent products elute from the gel gives the DNA sequence.

 


Related Discussions:- Automated dna sequencing

Symptoms of constipation, Q. show the Symptoms of constipation? Have yo...

Q. show the Symptoms of constipation? Have you ever suffered from constipation? Do you recall the symptoms associated with the problem? Yes, the symptoms were specific to havin

State the terms complete alimentary tract, State the terms complete aliment...

State the terms complete alimentary tract, complete digestive tract and complete gut? A digestive tract which has an anal and oral opening. This adaptation allowed for a linear

Explain japanese encephalitis, Japanese encephalitis  Japanese encephal...

Japanese encephalitis  Japanese encephalitis is an uncommon but potentially fatal mosquito-borne viral disease that occurs in rural Asia, especially near pig farms and rice pad

Chapter-2, multiple choise question

multiple choise question

Bacteria is an obligate anaerobe, Q. What is signifying when it is said tha...

Q. What is signifying when it is said that a bacteria is an obligate anaerobe? Obligate anaerobes are those living beings that do not survive in the presence of oxygen. For exa

Female reproductive system - vagina, V AGIN A - Median, elastic, musc...

V AGIN A - Median, elastic, muscular tube 7.5 cm long. Open into vestibule by vaginal orffice. The lining forms vaginal rugae. Space between vaginal wall & cervix is forn

Difference between that is alive, Is there a difference between something t...

Is there a difference between something that is alive and something that is a living organism?

What is the endocrine function of the placenta, What is the endocrine funct...

What is the endocrine function of the placenta? The placenta besides being the organ by which the exchange of substances among the mother and the fetus is done also has the fun

Why is meiosis important for the maintenance, Why is meiosis important for ...

Why is meiosis important for the maintenance of the normal quantity of chromosomes of a species with sexual reproduction? A reduction to a half of the maximum normal quantity o

Nitrification - nutrient cycles, Nitrification - Nutrient Cycles Ammon...

Nitrification - Nutrient Cycles Ammonia or ammonium salts, are converted into nitrate in a process termed nitrification to be useful to most autotrophic and heterotrophic orga

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd