Automated dna sequencing, Biology

Assignment Help:

Automated DNA sequencing is now common place which is based on the chain termination method but using a fluorescent dye attached to an oligonucleotide primer instead of using radioactive labeling.  A several  fluorescent  dye is attached  to the primer  for every  of the four  sequencing  reactions  but,  follow  incubation,  all four combinations  are electrophoresed    and combined on one gel lane. An laser detection  systems  then  distinguish  the  self  of  every  termination  product  as  it elutes  from  the gel. The sequence in that the several fluorescent products elute from the gel gives the DNA sequence.

 


Related Discussions:- Automated dna sequencing

Immunity, We have no antibodies against any blood group when we are born st...

We have no antibodies against any blood group when we are born still we say that some blood groups are incompatible for us.why?

Use of computer in biotechnology, what are the uses of computer in biotechn...

what are the uses of computer in biotechnology ??? briefly explain any twenty

Explain genetically modified foods, Normal 0 false false fa...

Normal 0 false false false EN-US X-NONE X-NONE MicrosoftInternetExplorer4

The right side of the container, A solute passes from higher concentration ...

A solute passes from higher concentration on the left of a container to the right side of container thru a membrane. What would happen if a second solute of lower concentration was

Introduction to ecosystem, The terms ecosystem was introduction by Sir Arth...

The terms ecosystem was introduction by Sir Arthur tensely in 1935. An ecosystem can be defined s " a structural and function unit of biosphere consisting of living organisms and t

What are respiratory pigments, What are respiratory pigments? What is the r...

What are respiratory pigments? What is the respiratory pigment present in some arthropods? Which is the analogous molecule in humans? Respiratory pigments are molecules able t

Production of disease-free plants, Production of Disease-free Plants U...

Production of Disease-free Plants Under normal conditions plants are infected by a wide range of pathogens such as bacteria, fungi, viruses, viroids, and insects like nematode

Atp, ATP The energy currency of the body One of the monomers u...

ATP The energy currency of the body One of the monomers used in the synthesis of RNA Contains three phosphate groups àhigh energy Removal of one releases e

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd