Automated dna sequencing, Biology

Assignment Help:

Automated DNA sequencing is now common place which is based on the chain termination method but using a fluorescent dye attached to an oligonucleotide primer instead of using radioactive labeling.  A several  fluorescent  dye is attached  to the primer  for every  of the four  sequencing  reactions  but,  follow  incubation,  all four combinations  are electrophoresed    and combined on one gel lane. An laser detection  systems  then  distinguish  the  self  of  every  termination  product  as  it elutes  from  the gel. The sequence in that the several fluorescent products elute from the gel gives the DNA sequence.

 


Related Discussions:- Automated dna sequencing

What is the futile cycle, What is the futile cycle The futile cycle is ...

What is the futile cycle The futile cycle is avoided because covalent modification, by  phosphorylation, has opposite effects on  the  enzymes concerned with  the  synthesis  a

Explain about the insulin syringe, Insulin Syringe To administer Insuli...

Insulin Syringe To administer Insulin properly, familiarize yourself with information about insulin: (a) insulin syringes, (b) storage, (c) site selection (d) steps for injecti

How does the excretory system of nematodes work, Q How does the excretory s...

Q How does the excretory system of nematodes work? The metabolic residuals of nematodes are together by two longitudinal lateral excretory channels that open in one single excr

Symptoms of enteropathogenic escherichia coli, Symptoms: The E. coli gastro...

Symptoms: The E. coli gastroenteritis syndrome is caused by the ingestion of 10 6 -10 10 viable cells/g that must colonize the small intestine and produce  enterotoxin. The syndr

How can the immune memory lead to the efficacy of vaccines, How can the imm...

How can the immune memory lead to the efficacy of vaccines and also produce allergies? Vaccines are controlled inoculations of fragments of infectious agents or of inactive inf

Mitochondria be considered the power plants of the cell, Q. Why can mitocho...

Q. Why can mitochondria be considered the power plants of the aerobic cells? Mitochondria are the "power plants" of aerobic cells because within them the final stages of the ce

Protein case study, DK is a year old female who was born after a normal pre...

DK is a year old female who was born after a normal pregnancy and delivery.  At 17 months of age DK showed signs of speech delay, increased lethargy and vomiting up to four times p

Dispersal of seeds , Dispersal of Seeds A plant usually bears many fr...

Dispersal of Seeds A plant usually bears many fruits and innumerable seeds. If all the seeds produced by a plant were to germinate in the immediate vicinity, this will have s

Fast response - biological responses, Fast Response - Biological Responses ...

Fast Response - Biological Responses Some of the fast responses are discussed below: It was found that when mung bean root tips were kept in a specific solution (contain

Define rheology and syneresis, Define Rheology and syneresis Rheology:...

Define Rheology and syneresis Rheology:   the science of the deformation and flow of matter. It is the branch of physics concerned with the flow and change of shape of matter,

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd