Automated dna sequencing, Biology

Assignment Help:

Automated DNA sequencing is now common place which is based on the chain termination method but using a fluorescent dye attached to an oligonucleotide primer instead of using radioactive labeling.  A several  fluorescent  dye is attached  to the primer  for every  of the four  sequencing  reactions  but,  follow  incubation,  all four combinations  are electrophoresed    and combined on one gel lane. An laser detection  systems  then  distinguish  the  self  of  every  termination  product  as  it elutes  from  the gel. The sequence in that the several fluorescent products elute from the gel gives the DNA sequence.

 


Related Discussions:- Automated dna sequencing

Explain about hyperglycemia, Q. Explain about Hyperglycemia? It is a Gr...

Q. Explain about Hyperglycemia? It is a Greek term: hyper -meaning excessive; glyc- meaning sweet; and emia- means "of the blood". It is a condition in which an excessive amoun

What is vegetarianism, What is Vegetarianism? Protein quality of the ...

What is Vegetarianism? Protein quality of the vegetarian diets can be improved by proper diet planning, However, under free-living conditions, vegetarianism can limit pr

., what is the skeleton in the different classes of coelentrata known

what is the skeleton in the different classes of coelentrata known

Define food science and simple sugars, Define Food Science and Simple Sugar...

Define Food Science and Simple Sugars? This first unit introduces the concept, scope and importance of food science and technology as a discipline. It further presents a detail

Is expression of cloned gene always a success, Question 1 Is expression of...

Question 1 Is expression of cloned gene always a success? How does the method of molecular photo copying work? Question 2 Explain the control of gene expression with the exam

Blastulation, BLASTUL A TIO N - Type of blastula is blastocyst or...

BLASTUL A TIO N - Type of blastula is blastocyst or trophoblastula. Outer layer of cell becomes flat and form trophoblast or trophoectoderm which receives nutritive ma

What are the main human diseases caused by prions, What are the main human ...

What are the main human diseases caused by prions? The main called human diseases of such type are the Creutzfeldt-Jacob disease (CJD), the kuru and the Gerstmann-Sträussle-Sch

What is a terrestrial organism, What is a terrestrial organism? 'Terra'...

What is a terrestrial organism? 'Terra' is the Latin word for earth. Thus, an animal that lives on the surface of the earth is known as terrestrial. This is the similar root wo

Illustrate about dosage and administration of menactra, Dosage and  adminis...

Dosage and  administration  Menactra is given as a single intramuscular injection. The need for and timing of booster doses is unknown. An unpublished study summarized in the p

How can we control the disease cholera, How can we control the disease Chol...

How can we control the disease Cholera Control: Individuals infected  with cholera require oral rehydration therapy  with NaCl plus sucrose, sodium bicarbonate and potassium ch

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd