Automated dna sequencing, Biology

Assignment Help:

Automated DNA sequencing is now common place which is based on the chain termination method but using a fluorescent dye attached to an oligonucleotide primer instead of using radioactive labeling.  A several  fluorescent  dye is attached  to the primer  for every  of the four  sequencing  reactions  but,  follow  incubation,  all four combinations  are electrophoresed    and combined on one gel lane. An laser detection  systems  then  distinguish  the  self  of  every  termination  product  as  it elutes  from  the gel. The sequence in that the several fluorescent products elute from the gel gives the DNA sequence.

 


Related Discussions:- Automated dna sequencing

Subphylum uniramia, Subphylum Uniramia Uniramia, the largest subphylu...

Subphylum Uniramia Uniramia, the largest subphylum of phylum Arthropods includes myriapods and insects. Due to the unbranched nature of the appendages of its members, the sub

Hemoglobin binds o2 cooperatively, Which of the following statements is cor...

Which of the following statements is correct? A. Hemoglobin binds O2 cooperatively, while myoglobin does not. B. Myoglobin binds O2 cooperatively, while hemoglobin does not.

Properties of foods, Q. Properties of foods? various properties of food...

Q. Properties of foods? various properties of foods, under which the quality attributes of foods were introduced to you in terms of food quality and its categories as appearanc

Explain germ layers that form tissues and organs in animals, Q. What are th...

Q. What are the three types of germ layers that form tissues and organs in animals? The three germ layers are the mesoderm, the ectoderm and the endoderm.

What is the importance of vitamin B2, What is the Importance of vitamin b2 ...

What is the Importance of vitamin b2 As a prosthetic group of the flavin enzymes, vitamin B 2 is involved in the reactions of almost all nutrients of plants and animals. They

Embryology in relation to taxonomy, Q. Embryology in relation to taxonomy? ...

Q. Embryology in relation to taxonomy? Embryological information has been used for taxonomic purposes at various levels of classification. You know of a very basic division of

Chemical stress - nature of the solutes, Chemical Stress - Nature of the so...

Chemical Stress - Nature of the solutes The acidic or basic reaction of soil and water of a particular habitat reflects its geochemical history beginning with its formation an

Illustrate the advantages of ridgemapping, Advantages of Ridgemapping T...

Advantages of Ridgemapping This procedure is a good indicator of the available bone width and helps in understanding the angulation of the available bone to the eventual prosth

Nucleic acids, Nucleic Acids Friedrich Miescher  (1868) discovered the ...

Nucleic Acids Friedrich Miescher  (1868) discovered the presence  of these compound  in protoplasm ,but Altman (1889) introduced the term Nucleic acid. These  acids are the lar

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd