Automated dna sequencing, Biology

Assignment Help:

Automated DNA sequencing is now common place which is based on the chain termination method but using a fluorescent dye attached to an oligonucleotide primer instead of using radioactive labeling.  A several  fluorescent  dye is attached  to the primer  for every  of the four  sequencing  reactions  but,  follow  incubation,  all four combinations  are electrophoresed    and combined on one gel lane. An laser detection  systems  then  distinguish  the  self  of  every  termination  product  as  it elutes  from  the gel. The sequence in that the several fluorescent products elute from the gel gives the DNA sequence.

 


Related Discussions:- Automated dna sequencing

Evolution and growth of sports nutrition as a discipline, Define Evolution ...

Define Evolution and Growth of Sports Nutrition as a Discipline? An academic discipline is basically a formal body of knowledge discovered, developed, and disseminated through

What are gametes, What are gametes? Gametes are cells specialized in s...

What are gametes? Gametes are cells specialized in sexual reproduction. They have half of the maximum number of chromosomes of the species and unite with another gamete giving

Molecule that transports oxygen in red blood cells, Q. What is the name of ...

Q. What is the name of the molecule that transports oxygen in red blood cells? The respiratory pigment of the red blood cells is hemoglobin.

Composition of capillaries, Normal 0 false false false ...

Normal 0 false false false EN-IN X-NONE X-NONE MicrosoftInternetExplorer4

Living Environment- Cells, What happens to a cell when placed in a salt sol...

What happens to a cell when placed in a salt solution and then into distilled water?

Phosphorylation, Phosphorylation is the addition of the phosphate monoeste...

Phosphorylation is the addition of the phosphate monoester to a macromolecule, catalyzed by a particular kinase enzyme. With respect to the proteins, particular amino acid side ch

Benthos - aquatic ecosystem, Benthos - Aquatic Ecosystem The benthos o...

Benthos - Aquatic Ecosystem The benthos or the benthic organisms are those found living in or on the bottom or benthic region of the water mass. They exhibit a variety of adap

Explain root amputation, Explain the Root amputation, Hemisectioning and Bi...

Explain the Root amputation, Hemisectioning and Bicuspidization Root amputation: removal of one or more roots of a multirooted tooth "upper teeth". Hemisectioning: surgic

Chemical bond maintains the pairing of each chain, Which type of chemical b...

Which type of chemical bond maintains the pairing of each chain in the DNA molecule? Ans) To form the DNA molecule, purine bases bind to pyrimidine bases by intermolecular bonds

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd