Automated dna sequencing, Biology

Assignment Help:

Automated DNA sequencing is now common place which is based on the chain termination method but using a fluorescent dye attached to an oligonucleotide primer instead of using radioactive labeling.  A several  fluorescent  dye is attached  to the primer  for every  of the four  sequencing  reactions  but,  follow  incubation,  all four combinations  are electrophoresed    and combined on one gel lane. An laser detection  systems  then  distinguish  the  self  of  every  termination  product  as  it elutes  from  the gel. The sequence in that the several fluorescent products elute from the gel gives the DNA sequence.

 


Related Discussions:- Automated dna sequencing

Phylogeny, why do i do it for class/

why do i do it for class/

Causes of solid wastes, The main causes for the rapid growth in the quantit...

The main causes for the rapid growth in the quantity of solid wastes are- (i)     Over population: the ever increasing population is increasing all types of pollution; same is t

Criticism of darwinism, CRITICISM OF DARWINISM - Arriva l of the f...

CRITICISM OF DARWINISM - Arriva l of the fittest: Darwinism explains the survival of the fittest but was unable to account for the arrival of the fittest. V estigia

Define assessment of chromium status, Define Assessment of Chromium Status?...

Define Assessment of Chromium Status? No specific tests are currently available, which could help us to determine chromium status. Another reason being the chromium content of

Give a specific example for epithelial, PROVIDE a specific example for epit...

PROVIDE a specific example for epithelial and connective tissues, how the arrangement of cells helps with tissue functioning?

Define about oxygen molecule, How many pairs of electrons do the two oxygen...

How many pairs of electrons do the two oxygen atoms in an oxygen molecule share with each  other? Explain your answer. The oxygen atoms share two pairs of electrons, sinc

Hirudinea - feeding and digestion in annelids, Hirudinea - Feeding and Dige...

Hirudinea - Feeding and Digestion in Annelids Hirudinea involves free living and ectoparasitic leeches. Leeches are blood suckers. The digestive system contains a preoral cham

Energy input in ecosystem, Energy Input in Ecosystem Survival and func...

Energy Input in Ecosystem Survival and functioning of ecosystem is dependent on the input of energy. Continuous availability of energy is essential for supporting diverse ecos

Action of light the basis of the photosynthesis process, Is it correct to c...

Is it correct to consider water decomposition by the action of light the basis of the photosynthesis process? Besides ADP photophosphorylation, photic energy is also responsibl

Fertilization - pre-embryonic development, Fertilization - Pre-Embryonic De...

Fertilization - Pre-Embryonic Development Ovulation generally takes place between 9th to 14th day after the end of last menstrual period. Fertilization of oocyte initiates dev

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd