Automated dna sequencing, Biology

Assignment Help:

Automated DNA sequencing is now common place which is based on the chain termination method but using a fluorescent dye attached to an oligonucleotide primer instead of using radioactive labeling.  A several  fluorescent  dye is attached  to the primer  for every  of the four  sequencing  reactions  but,  follow  incubation,  all four combinations  are electrophoresed    and combined on one gel lane. An laser detection  systems  then  distinguish  the  self  of  every  termination  product  as  it elutes  from  the gel. The sequence in that the several fluorescent products elute from the gel gives the DNA sequence.

 


Related Discussions:- Automated dna sequencing

Nutrition - different feeding habits, Different Feeding Habits Feeding ...

Different Feeding Habits Feeding habits of animals depend on the nature of the food consumed. Herbivorous : When plants are eaten. Examples - Horse, cow, rabbit, apes.

Preventive steps for global warming, Preventive steps for global warming : ...

Preventive steps for global warming :  Growing trees in vacant places. Growing social forests and protect them. Promoting abundant growth of phytoplankton plant species in marine

Chitra valve-types of valves, Chitra Valve :  This valve was desi...

Chitra Valve :  This valve was designed and developed at Sri.Chitra Institute of Medical Sciences in Thiruvananthapuram and marketed by TTK Industries. It is a tilting di

How dissimilar are cnidarians from poriferans, Q. Concerning tissue complex...

Q. Concerning tissue complexity how dissimilar are cnidarians from poriferans? Poriferans present only some dispersed specialized cells with no tissue differentiation, Cnidaria

Brain region responsible for the equilibrium of the body, Q. Which is the b...

Q. Which is the brain region responsible for the coordination and equilibrium of the body? In the central nervous system the cerebellum is the main controller of the motor equi

What is the life cycle of ascaris, Q. What is the life cycle of ascaris? ...

Q. What is the life cycle of ascaris? The Adult ascaris that live within the human intestine can release up to 200 thousand eggs a day. The eggs are eradicating with human fece

Explain japanese encephalitis, Japanese encephalitis  Japanese encephal...

Japanese encephalitis  Japanese encephalitis is an uncommon but potentially fatal mosquito-borne viral disease that occurs in rural Asia, especially near pig farms and rice pad

Disorders related to nutrition and digestive system, DISORDE R RELATED TO ...

DISORDE R RELATED TO NUTRITION AND DIGESTIVE SYSTEM Malnutrition Malnutrition is improper intake of food. Malnutrion covers both undernutrition and overnutrition. Und

Embrylogical devlopment of hypospadias, Embrylogical Devlopment of Hypos...

Embrylogical Devlopment of Hypospadias The development of the penis and scrotum is intluenced by the testes. In the absence of the testes, the female structures of the

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd