Atomic fluorescence spectrometry, Chemistry

Assignment Help:

Atomic fluorescence spectrometry:

In atomic fluorescence spectrometry, the gaseous atoms acquired through flame or electrothermal atomisation are excited to higher energy levels through absorption of the electromagnetic radiation and the fluorescence emission from these excited atoms is measured. A fluorescence emission could occur by different pathways. Therefore, we have various types of atomic fluorescence transitions. The general types of atomic fluorescence transitions are termed as resonance fluorescence, Stokes direct line fluorescence, stepwise line fluorescence, two step excitation or double resonance fluorescence, sensitized fluorescence and thermal fluorescence. Of these, the thermally assisted fluorescence and sensitized fluorescence commonly are not employed for analytical reasons.

The intensity of the fluorescence radiation is measured at right angles to the direction of incident radiation and is associated to the concentration of the element present, creating the basis of quantitative analysis. In quantitative atomic fluorescence spectrometric determinations the instrument is commonly standardised through a calibration curve. The graph is drawn among the logarithms of the intensity of atomic fluorescence signal versus the log of analyte concentration. The linearity of like curves extends over 3 to 5 sequences of magnitudes. Thus, at higher concentration, linearity is lost because of self absorption.


Related Discussions:- Atomic fluorescence spectrometry

Ligand field theory, LIGAND FIELD THEORY The five d orbitals with dist...

LIGAND FIELD THEORY The five d orbitals with distinct values of the magnetic quantum number (m) have the similar energy in a free atom or ion. In any compound they interact

Benzoic acid, benzoic acid from benzene.i can''t see it clearly

benzoic acid from benzene.i can''t see it clearly

Nomenclature of simple aromatic compounds, Nomenclature of simple aromatic ...

Nomenclature of simple aromatic compounds Aromatic compounds are those that consist of one or more benzene rings in them. An aromatic substance has two major types: (1) Nucl

Lime soda process numerical (water treatment), While solving lime sida nume...

While solving lime sida numericals we calculate CACO3 equivalent .In this we do conc. In ppm×100/mol. Wt. ssomewhere we multiply 2 × m. Wt .so wht 2

AP Thermodynamics, Given that ?H°298 for the reaction is -92.2 kJ mol-1, wh...

Given that ?H°298 for the reaction is -92.2 kJ mol-1, which is larger, the total bond dissociation energy of the reactants or the total bond dissociation energy of the products?

Molecar symetry, Explain significance of orthogonality rule

Explain significance of orthogonality rule

Ionic compounds do not have, Ionic compounds do not have: (1) Hard and ...

Ionic compounds do not have: (1) Hard and brittle nature (2) High melting and boiling point (3) Directional properties (4) Soluble in polar solvents Ans:  Direction

Difference between oraganic and inoraganic compounds, An organic compound i...

An organic compound is any member of a large class of chemical compounds which molecules have carbon and hydrogen; thus, carbides, carbonates, carbon oxides and elementary carbon

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd