Atomic fluorescence spectrometry, Chemistry

Assignment Help:

Atomic fluorescence spectrometry:

In atomic fluorescence spectrometry, the gaseous atoms acquired through flame or electrothermal atomisation are excited to higher energy levels through absorption of the electromagnetic radiation and the fluorescence emission from these excited atoms is measured. A fluorescence emission could occur by different pathways. Therefore, we have various types of atomic fluorescence transitions. The general types of atomic fluorescence transitions are termed as resonance fluorescence, Stokes direct line fluorescence, stepwise line fluorescence, two step excitation or double resonance fluorescence, sensitized fluorescence and thermal fluorescence. Of these, the thermally assisted fluorescence and sensitized fluorescence commonly are not employed for analytical reasons.

The intensity of the fluorescence radiation is measured at right angles to the direction of incident radiation and is associated to the concentration of the element present, creating the basis of quantitative analysis. In quantitative atomic fluorescence spectrometric determinations the instrument is commonly standardised through a calibration curve. The graph is drawn among the logarithms of the intensity of atomic fluorescence signal versus the log of analyte concentration. The linearity of like curves extends over 3 to 5 sequences of magnitudes. Thus, at higher concentration, linearity is lost because of self absorption.


Related Discussions:- Atomic fluorescence spectrometry

The Effect of Temperature and the Collision Model, A reaction has a rate co...

A reaction has a rate constant of 1.22x10^-4 /s at 28 C and 0.230 /s at 75.0 C. 1. Determine the activation barrier for the reaction in kj/mol. 2. What is the value of the rate co

Symmetry, character table symmetry element ammonia

character table symmetry element ammonia

Physical properties of malonic acid, Physical Properties of Malonic Acid ...

Physical Properties of Malonic Acid (a) Malonic Acid is a white crystalline solid. (b) Malonic Acid's melting point is 135°C. (c) Malonic Acid is soluble in water and alc

Determine spherical symmetry, A filled or half-filled set of p or d  -orbit...

A filled or half-filled set of p or d  -orbitals is spherically symmetric. Point out the species which has spherical symmetry: (1) Na                    (2) C

Tie line, tie line explain in with the help of phase rule

tie line explain in with the help of phase rule

When an electron jumps from lower to higher orbit energy, When an electron ...

When an electron jumps from lower to higher orbit, its energy : (1) Increases (2) Decreases (3) Remains the same (4) None of these Ans:  Increases

What is the basic structure of alcohols, Q. What is the basic structure of ...

Q. What is the basic structure of Alkynes? Ans. Alcohols have the general chemical formula of ROH. The R stands for the rest of the molecule and is usually an alkyl group

Draw the structure of xef2 molecule, a) Give a chemical test to distinguish...

a) Give a chemical test to distinguish between aniline and N-methyl aniline. Draw the structure of XeF2 molecule. b. Write the outer electronic configuration of Cr atom (Z =

Freezing Point Depression, A solution is prepared with 3.85g of a non-ionic...

A solution is prepared with 3.85g of a non-ionic unknown compound in 100.0g cyclohexane. The solution had a freezing point of 4.18 degrees Celsius. What is the molar mass of the un

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd