Atomic fluorescence spectrometry, Chemistry

Assignment Help:

Atomic fluorescence spectrometry:

In atomic fluorescence spectrometry, the gaseous atoms acquired through flame or electrothermal atomisation are excited to higher energy levels through absorption of the electromagnetic radiation and the fluorescence emission from these excited atoms is measured. A fluorescence emission could occur by different pathways. Therefore, we have various types of atomic fluorescence transitions. The general types of atomic fluorescence transitions are termed as resonance fluorescence, Stokes direct line fluorescence, stepwise line fluorescence, two step excitation or double resonance fluorescence, sensitized fluorescence and thermal fluorescence. Of these, the thermally assisted fluorescence and sensitized fluorescence commonly are not employed for analytical reasons.

The intensity of the fluorescence radiation is measured at right angles to the direction of incident radiation and is associated to the concentration of the element present, creating the basis of quantitative analysis. In quantitative atomic fluorescence spectrometric determinations the instrument is commonly standardised through a calibration curve. The graph is drawn among the logarithms of the intensity of atomic fluorescence signal versus the log of analyte concentration. The linearity of like curves extends over 3 to 5 sequences of magnitudes. Thus, at higher concentration, linearity is lost because of self absorption.


Related Discussions:- Atomic fluorescence spectrometry

Redox chemistry, Reactions that are classified as oxidation-reduction (redo...

Reactions that are classified as oxidation-reduction (redox) reactions all have the common trait that electrons leave one component (that is, getting oxidized) to be taken up by an

Chemical methods - purification of organic compounds, Chemical methods - Pu...

Chemical methods - Purification of organic compounds Besides these physical methods, few chemical methods have as well been utilized to separate a mixture of organic compounds.

Composition of hydrocarbon, Composition of hydrocarbon (i) Alkanes: fou...

Composition of hydrocarbon (i) Alkanes: found 30 to 70% consist of upto 40 carbon molecule. Alkanes are mainly linear chain but some are branched chain isomers. (ii) Cycloal

Hydroxides, Why do zinc hydroxide, aluminium hydroxide and lead (II) hydrox...

Why do zinc hydroxide, aluminium hydroxide and lead (II) hydroxide dissolve in excess aqueous sodium hydroxide?

Find the concentration of hydrogen ions, An aqueous solution has a hydroxyl...

An aqueous solution has a hydroxyl concentration of 1.75 x 10^-7mol/L. Find the concentration of hydrogen ions in this solution.

What is carcinogenic substance?, Polynuclear benzenoid hydrocarbons are pro...

Polynuclear benzenoid hydrocarbons are provided to be carcinogenic that means cancer producing.There are liberated by incomplete combusion of certain substances like tobacoo,coal p

S block element, What is differences by alkali metal and alkaine earth meta...

What is differences by alkali metal and alkaine earth metal which provided colour

Draw an energy diagram, Figure states energy differences for the combustion...

Figure states energy differences for the combustion of hydrogen, an exothermic chemical reaction. The combination of oxygen gas and nitrogen gas to form nitrogen monoxide is an exa

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd