Atomic fluorescence spectrometry, Chemistry

Assignment Help:

Atomic fluorescence spectrometry:

In atomic fluorescence spectrometry, the gaseous atoms acquired through flame or electrothermal atomisation are excited to higher energy levels through absorption of the electromagnetic radiation and the fluorescence emission from these excited atoms is measured. A fluorescence emission could occur by different pathways. Therefore, we have various types of atomic fluorescence transitions. The general types of atomic fluorescence transitions are termed as resonance fluorescence, Stokes direct line fluorescence, stepwise line fluorescence, two step excitation or double resonance fluorescence, sensitized fluorescence and thermal fluorescence. Of these, the thermally assisted fluorescence and sensitized fluorescence commonly are not employed for analytical reasons.

The intensity of the fluorescence radiation is measured at right angles to the direction of incident radiation and is associated to the concentration of the element present, creating the basis of quantitative analysis. In quantitative atomic fluorescence spectrometric determinations the instrument is commonly standardised through a calibration curve. The graph is drawn among the logarithms of the intensity of atomic fluorescence signal versus the log of analyte concentration. The linearity of like curves extends over 3 to 5 sequences of magnitudes. Thus, at higher concentration, linearity is lost because of self absorption.


Related Discussions:- Atomic fluorescence spectrometry

Food chemistry, discuss the dietary functional properties of calcium in foo...

discuss the dietary functional properties of calcium in foods

How is harvest maturity identified, How is harvest maturity identified? ...

How is harvest maturity identified? Before harvesting, the first thing to be looked into is the identification of harvest maturity. We have already seen what the term ac

Kinetics and equilibrium, 2NO(g)+2H2O yeilds N2+2H2O Write the rate law for...

2NO(g)+2H2O yeilds N2+2H2O Write the rate law for the pervious reaction if doubling the concentration of H2 doubles the reaction rate, and if doubling the concentration of NO incre

Biochemisty, High energy bond in the intermediary metabolism

High energy bond in the intermediary metabolism

Stephen reaction - aldehydes and ketones, Stephen reaction - Aldehydes and ...

Stephen reaction - Aldehydes and Ketones Benzaldehyde is acquired by partial reduction of phenyl cyanide along with stannous chloride and passing dry HCl gas in ether solution

Why acetic has less conductivity than hcl, Acetic acid has less conductivit...

Acetic acid has less conductivity, due to it ionizes less in solution. HCl divides almost completely in solution to form the ions Cl- and H+..

Classification of organic compounds, Q. Classification of Organic Compounds...

Q. Classification of Organic Compounds?Groups of Organic Compounds? Ans. Organic compounds are classified on the basis of their functional groups. The common groups of org

Coupling reaction - miscellaneous reactions, Coupling reaction - Miscellane...

Coupling reaction - Miscellaneous reactions Phenol couples along with benzene diazonium chloride in existence of an alkaline solution to make a red dye (p-hydroxy azobenzene).

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd