Atomic fluorescence spectrometry, Chemistry

Assignment Help:

Atomic fluorescence spectrometry:

In atomic fluorescence spectrometry, the gaseous atoms acquired through flame or electrothermal atomisation are excited to higher energy levels through absorption of the electromagnetic radiation and the fluorescence emission from these excited atoms is measured. A fluorescence emission could occur by different pathways. Therefore, we have various types of atomic fluorescence transitions. The general types of atomic fluorescence transitions are termed as resonance fluorescence, Stokes direct line fluorescence, stepwise line fluorescence, two step excitation or double resonance fluorescence, sensitized fluorescence and thermal fluorescence. Of these, the thermally assisted fluorescence and sensitized fluorescence commonly are not employed for analytical reasons.

The intensity of the fluorescence radiation is measured at right angles to the direction of incident radiation and is associated to the concentration of the element present, creating the basis of quantitative analysis. In quantitative atomic fluorescence spectrometric determinations the instrument is commonly standardised through a calibration curve. The graph is drawn among the logarithms of the intensity of atomic fluorescence signal versus the log of analyte concentration. The linearity of like curves extends over 3 to 5 sequences of magnitudes. Thus, at higher concentration, linearity is lost because of self absorption.


Related Discussions:- Atomic fluorescence spectrometry

Gum, what makes gum chewy

what makes gum chewy

Chemical bonding, how does chemical bonding takes place?

how does chemical bonding takes place?

Where is tin obtained, Where is tin obtained? Ans) Tin is obtained in se...

Where is tin obtained? Ans) Tin is obtained in several places tin is found mainly in the ore cassiterite, which is originate in Malaysia, Bolivia, Thailand, and Nigeria.

Explain the term "scale up ", Scale up At this phase, sensory analysis...

Scale up At this phase, sensory analysis consists of tests that compare the production samples with the optimized product. Depending on the resources available, either consume

Normal functioning of blood, Q. Normal functioning of blood? The sixth ...

Q. Normal functioning of blood? The sixth position marked by X is either occupied by H 2 0 or 0 2 molecules. Impotant thing to note is that Fe 2+ in hemoglobin does not get o

What is vulcanization, What is vulcanization The process of vulcanizati...

What is vulcanization The process of vulcanization was discovered by Charles Good Year in 1839. He found that when natural rubber is heated with sulphur, its tensile strength,

Cycloalkane, formation of propane by parkin reaction

formation of propane by parkin reaction

Chemical properties of ethers, Chemical Properties of Ethers Ethers are...

Chemical Properties of Ethers Ethers are fairly stable compounds. These are not simply reacted along with alkalies, dilute mineral acids, active metals, oxidising agents within

Degree of superheat, How to know degree of superheat from psychometric char...

How to know degree of superheat from psychometric chart?

Hardness of water, Why does calcium sulphate form scales in high pressure b...

Why does calcium sulphate form scales in high pressure boilers

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd