Atomic fluorescence spectrometry, Chemistry

Assignment Help:

Atomic fluorescence spectrometry:

In atomic fluorescence spectrometry, the gaseous atoms acquired through flame or electrothermal atomisation are excited to higher energy levels through absorption of the electromagnetic radiation and the fluorescence emission from these excited atoms is measured. A fluorescence emission could occur by different pathways. Therefore, we have various types of atomic fluorescence transitions. The general types of atomic fluorescence transitions are termed as resonance fluorescence, Stokes direct line fluorescence, stepwise line fluorescence, two step excitation or double resonance fluorescence, sensitized fluorescence and thermal fluorescence. Of these, the thermally assisted fluorescence and sensitized fluorescence commonly are not employed for analytical reasons.

The intensity of the fluorescence radiation is measured at right angles to the direction of incident radiation and is associated to the concentration of the element present, creating the basis of quantitative analysis. In quantitative atomic fluorescence spectrometric determinations the instrument is commonly standardised through a calibration curve. The graph is drawn among the logarithms of the intensity of atomic fluorescence signal versus the log of analyte concentration. The linearity of like curves extends over 3 to 5 sequences of magnitudes. Thus, at higher concentration, linearity is lost because of self absorption.


Related Discussions:- Atomic fluorescence spectrometry

Coordination compounds, what is the name of this complex [Br2Pt(S Me)2PtBr2...

what is the name of this complex [Br2Pt(S Me)2PtBr2] and what is the formula of this compound pentamminecrbonato cobalt(III) tetrathiocyanato-N-zincate(II)

Proportional Error, Which sample is better to use, bigger sample or smaller...

Which sample is better to use, bigger sample or smaller sample?

Purge and recycle stream, The fresh feed to an ammonia production process c...

The fresh feed to an ammonia production process contains nitrogen (N2) and hydrogen (H2) in stoichiometric proportion, along with an inert gas (I). The feed is combined with a re

Blow moulding and thermoforming extensively, write on blow moulding and the...

write on blow moulding and thermoforming extensively. 3 pages

Gattermann-koch aldehyde synthesis, Gattermann-koch aldehyde synthesis ...

Gattermann-koch aldehyde synthesis Benzene is transformed into benzaldehyde by passing a mixture of carbon monoxide and HCl gas within high pressure into the ether solution of

Organic, show how you would sythesise the following compounds, starting wit...

show how you would sythesise the following compounds, starting with benzene or toluene and any necessary acyclic reagents. Assume para is the major product.

LATIMER, WHAT IS THE FEROST DIAGRAM

WHAT IS THE FEROST DIAGRAM

Lithium and beryllium, lithium and beryllium markedly differ from other mem...

lithium and beryllium markedly differ from other members of their respective groups

Cycloalkanes, Why cyclo propane is more stable than cyclohexane

Why cyclo propane is more stable than cyclohexane

F-block elements, similarities between the latter actinides and latter lant...

similarities between the latter actinides and latter lanthanides

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd