Atomic fluorescence spectrometry, Chemistry

Assignment Help:

Atomic fluorescence spectrometry:

In atomic fluorescence spectrometry, the gaseous atoms acquired through flame or electrothermal atomisation are excited to higher energy levels through absorption of the electromagnetic radiation and the fluorescence emission from these excited atoms is measured. A fluorescence emission could occur by different pathways. Therefore, we have various types of atomic fluorescence transitions. The general types of atomic fluorescence transitions are termed as resonance fluorescence, Stokes direct line fluorescence, stepwise line fluorescence, two step excitation or double resonance fluorescence, sensitized fluorescence and thermal fluorescence. Of these, the thermally assisted fluorescence and sensitized fluorescence commonly are not employed for analytical reasons.

The intensity of the fluorescence radiation is measured at right angles to the direction of incident radiation and is associated to the concentration of the element present, creating the basis of quantitative analysis. In quantitative atomic fluorescence spectrometric determinations the instrument is commonly standardised through a calibration curve. The graph is drawn among the logarithms of the intensity of atomic fluorescence signal versus the log of analyte concentration. The linearity of like curves extends over 3 to 5 sequences of magnitudes. Thus, at higher concentration, linearity is lost because of self absorption.


Related Discussions:- Atomic fluorescence spectrometry

Calculate the heat loss by polystyrene insulation foam, Heat Conductivity ...

Heat Conductivity When considering the design of houses, the heat conductivity by walls, roofs, and the floor plays an significant role. The heat conductivities (λ) of some bui

Wavelength of a spectral line for an electronic transition, The wavelength ...

The wavelength of a spectral line for an electronic transition is inversely related to: (1) The number of electrons undergoing the transition (2) The nuclear charge of the a

Radiometric titration, instrumentation and applications of radiometric titr...

instrumentation and applications of radiometric titration

Applications of flame photometry, Applications of flame photometry: A ...

Applications of flame photometry: A few common applications of flame photometry are given below. Flame photometry could be used to detect the presence of the elements o

Chemistry in daily life - medicine, various types of medicines use in daily...

various types of medicines use in daily life? and classify them into analgesics, antipyretics, antibiotics, sedatives, antiseptics.

In bohr''s model, In Bohr's model, atomic radius of the first orbit is γ th...

In Bohr's model, atomic radius of the first orbit is γ the radius of the 3rd orbit, is: (1) γ/ 3 (2)γ (3) 3γ (4) 9γ Ans:  9γ

Branched chain polymers - classification of polymers, Branched chain polym...

Branched chain polymers -  Classification of polymers These are polymers where the monomers are connected to make long chains with side chains or branches of dissimilar sizes.

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd