Atomic fluorescence spectrometry, Chemistry

Assignment Help:

Atomic fluorescence spectrometry:

In atomic fluorescence spectrometry, the gaseous atoms acquired through flame or electrothermal atomisation are excited to higher energy levels through absorption of the electromagnetic radiation and the fluorescence emission from these excited atoms is measured. A fluorescence emission could occur by different pathways. Therefore, we have various types of atomic fluorescence transitions. The general types of atomic fluorescence transitions are termed as resonance fluorescence, Stokes direct line fluorescence, stepwise line fluorescence, two step excitation or double resonance fluorescence, sensitized fluorescence and thermal fluorescence. Of these, the thermally assisted fluorescence and sensitized fluorescence commonly are not employed for analytical reasons.

The intensity of the fluorescence radiation is measured at right angles to the direction of incident radiation and is associated to the concentration of the element present, creating the basis of quantitative analysis. In quantitative atomic fluorescence spectrometric determinations the instrument is commonly standardised through a calibration curve. The graph is drawn among the logarithms of the intensity of atomic fluorescence signal versus the log of analyte concentration. The linearity of like curves extends over 3 to 5 sequences of magnitudes. Thus, at higher concentration, linearity is lost because of self absorption.


Related Discussions:- Atomic fluorescence spectrometry

Illustrate relative amounts of two co-existing phases, Illustrate that...

Illustrate that  for  correct  mass  balance,  relative  amounts  of  two  co-existing  phases  or microconstituents must be given by lever principle. Ans:   Using  lever  rule

Acyl chloride , what is the mechanism of acyl chloride reaction with alcoho...

what is the mechanism of acyl chloride reaction with alcohol and why does it react in this way ?

Write the structure of teflon, Write the structures of monomers used in the...

Write the structures of monomers used in the preparation of: a. Teflon b. PMMA

p-methoxybenzyl bromide reacts with ethanol, Why does p-methoxybenzyl brom...

Why does p-methoxybenzyl bromide reacts with ethanol faster than p-nitrobenzylbromide Solution) Because o-ch 3 is electron donating group while NO 2 is a electron withdrawing g

Naming complex substituents - iupac system of nomenclature, Naming complex ...

Naming complex substituents - IUPAC system of nomenclature If the substituent on the parent chain is complex ( that is it is branched) it is termed as substituted alkyl g

Explain multimolecular and macromolecular colloids, What is the difference ...

What is the difference among multimolecular and macromolecular colloids? Give one example of each type. How are associated colloids dissimilar from these two types of colloids?

Explain the following, Lithium and beryllium markedly differ from other mem...

Lithium and beryllium markedly differ from other members of their respective groups? Read more: http://wiki.answers.com/Q/Lithium_and_beryllium_markedly_differ_from_other_members

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd