Atomic fluorescence spectrometry, Chemistry

Assignment Help:

Atomic fluorescence spectrometry:

In atomic fluorescence spectrometry, the gaseous atoms acquired through flame or electrothermal atomisation are excited to higher energy levels through absorption of the electromagnetic radiation and the fluorescence emission from these excited atoms is measured. A fluorescence emission could occur by different pathways. Therefore, we have various types of atomic fluorescence transitions. The general types of atomic fluorescence transitions are termed as resonance fluorescence, Stokes direct line fluorescence, stepwise line fluorescence, two step excitation or double resonance fluorescence, sensitized fluorescence and thermal fluorescence. Of these, the thermally assisted fluorescence and sensitized fluorescence commonly are not employed for analytical reasons.

The intensity of the fluorescence radiation is measured at right angles to the direction of incident radiation and is associated to the concentration of the element present, creating the basis of quantitative analysis. In quantitative atomic fluorescence spectrometric determinations the instrument is commonly standardised through a calibration curve. The graph is drawn among the logarithms of the intensity of atomic fluorescence signal versus the log of analyte concentration. The linearity of like curves extends over 3 to 5 sequences of magnitudes. Thus, at higher concentration, linearity is lost because of self absorption.


Related Discussions:- Atomic fluorescence spectrometry

Discuss about the different types of stones, Discuss about the different ty...

Discuss about the different types of stones used for the construction and how to determine the good quality stone?

Liquification of gases, tell me about liquification of gases in detail but ...

tell me about liquification of gases in detail but in most easy words

Luciferase/Luciferin Reaction Optimized Quantities, How would I go about cr...

How would I go about creating a luciferase and luciferin reaction? How do I find optimized quantity ratios? If I buy dehydrated luciferin or luciferase, how would I need to rehydra

Reichert-meissl value - analysis of oils and fats, Reichert-Meissl value o...

Reichert-Meissl value or R/M value - Analysis of oils and fats It points out the amount of steam volatile fatty acids exist in the oil or fat. It is illustarted as the number o

Simple distillation, n-propyl acetate (bp 102 C) evaporates rapidly when ex...

n-propyl acetate (bp 102 C) evaporates rapidly when exposed to air, but water(bp 100C)does not. Explain why

Arenes, the most stable structure of chrysene

the most stable structure of chrysene

Redox reactions, How do i balance the following equation: Ni+CO==>NI(CO)4? ...

How do i balance the following equation: Ni+CO==>NI(CO)4? I can''t figure out the oxidation state of Ni and C on the product side.

Summery, i wanna someone help me to do summery from 2 pages like essay for ...

i wanna someone help me to do summery from 2 pages like essay for articl?

Transition elements, Why transition elements form interstitial compound

Why transition elements form interstitial compound

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd