Atomic fluorescence spectrometry, Chemistry

Assignment Help:

Atomic fluorescence spectrometry:

In atomic fluorescence spectrometry, the gaseous atoms acquired through flame or electrothermal atomisation are excited to higher energy levels through absorption of the electromagnetic radiation and the fluorescence emission from these excited atoms is measured. A fluorescence emission could occur by different pathways. Therefore, we have various types of atomic fluorescence transitions. The general types of atomic fluorescence transitions are termed as resonance fluorescence, Stokes direct line fluorescence, stepwise line fluorescence, two step excitation or double resonance fluorescence, sensitized fluorescence and thermal fluorescence. Of these, the thermally assisted fluorescence and sensitized fluorescence commonly are not employed for analytical reasons.

The intensity of the fluorescence radiation is measured at right angles to the direction of incident radiation and is associated to the concentration of the element present, creating the basis of quantitative analysis. In quantitative atomic fluorescence spectrometric determinations the instrument is commonly standardised through a calibration curve. The graph is drawn among the logarithms of the intensity of atomic fluorescence signal versus the log of analyte concentration. The linearity of like curves extends over 3 to 5 sequences of magnitudes. Thus, at higher concentration, linearity is lost because of self absorption.


Related Discussions:- Atomic fluorescence spectrometry

PERIODIC TABLE, PERIODIC TABLE and PERIODICITY OF ELEMENT

PERIODIC TABLE and PERIODICITY OF ELEMENT

Insulating vessel containing negligible heat capacity, 2 Kg of ice at -20 d...

2 Kg of ice at -20 degree C is mixed with 5Kg of water 20 degree C in an insulating vessel containing negligible heat capacity. Measure the final mass of water remaining in the con

Study of elements of group 16, Q. Study of Elements of group 16? We sha...

Q. Study of Elements of group 16? We shall discuss Group 16 elements, viz., oxygen, sulphur, selenium, tellurium and polonium. These elements are called chalcogens or the ore f

The property is commonly exhibited by a covalent compound, Among the follow...

Among the following which property is commonly exhibited by a covalent compound: (1) High solubility in water           (2) High electrical conductance (3) Low boiling po

Determine atomic number and mass number, Q. Determine Atomic Number and Mas...

Q. Determine Atomic Number and Mass Number? One of the four naturally taking place isotopes of chromium has a mass number of 53. Determine the number of electrons, protons and

P-block element, show the characteristic of p-block element

show the characteristic of p-block element

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd