Atomic fluorescence spectrometry, Chemistry

Assignment Help:

Atomic fluorescence spectrometry:

In atomic fluorescence spectrometry, the gaseous atoms acquired through flame or electrothermal atomisation are excited to higher energy levels through absorption of the electromagnetic radiation and the fluorescence emission from these excited atoms is measured. A fluorescence emission could occur by different pathways. Therefore, we have various types of atomic fluorescence transitions. The general types of atomic fluorescence transitions are termed as resonance fluorescence, Stokes direct line fluorescence, stepwise line fluorescence, two step excitation or double resonance fluorescence, sensitized fluorescence and thermal fluorescence. Of these, the thermally assisted fluorescence and sensitized fluorescence commonly are not employed for analytical reasons.

The intensity of the fluorescence radiation is measured at right angles to the direction of incident radiation and is associated to the concentration of the element present, creating the basis of quantitative analysis. In quantitative atomic fluorescence spectrometric determinations the instrument is commonly standardised through a calibration curve. The graph is drawn among the logarithms of the intensity of atomic fluorescence signal versus the log of analyte concentration. The linearity of like curves extends over 3 to 5 sequences of magnitudes. Thus, at higher concentration, linearity is lost because of self absorption.


Related Discussions:- Atomic fluorescence spectrometry

Explain the structure of single covalent bonds, Q. Explain the structure of...

Q. Explain the structure of Single covalent bonds? Ans. Atoms tend to achieve the stable electron configuration of a noble gas when forming a bond. In covalent bonding thi

Filtrate preparation., how is the filtrate prepared in basic radical testin...

how is the filtrate prepared in basic radical testing

Difference between silica used in tlc and hplc column, What is difference b...

What is difference between silica used in TLC and HPLC column? TLC is called thin layer chromatography. The simple technique works on the principle of adsorption, where as HPLC i

Bad teacher, can you help me with understanding chemistry?

can you help me with understanding chemistry?

Complex formation-equilibrium constants, Equilibrium constants A complex...

Equilibrium constants A complex in general is any species build by specific association of molecules or ions by donor-acceptor interactions. In aqueous solutions the most import

Title: Organic, Show by means of equations and reagents, how the following ...

Show by means of equations and reagents, how the following conversion would be achieved? Propyne to butanoic acid

Hazards, What are the examples of radiation hazards

What are the examples of radiation hazards

Define degrees of freedom - phase rule and phase diagrams, Define Degrees o...

Define Degrees of freedom - phase rule and phase diagrams? Consider a system in an equilibrium state. In this state, the system has one or more phases; each phase contains one

Edta method, explain the principle of EDTA method

explain the principle of EDTA method

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd