Artificial skin, Biology

Assignment Help:

Artificial Skin:

The skin generated in vitro is in fact the epidermis portion of skin only; when the epidermis is applied to the burnt part, this leads to the regeneration of dermis (remaining parts of skin) beneath. But improvements in the approach  have allowed the reconstitution of virtually complete skin (both epidermis and dermis), called as living skin equivalent (LSE); this technology uses a collagen matrix as a support for development of the tissue. The skin explants used for attaining artificial skin may be either obtained from the patient concerned or from the foreskin (loose skin from the tip of penis) of newborn. Skin cells of new-borns grow more strongly than adult skin; the use of a synthetic polymer called as PGA helps the newborn skin to develop with no scars. Artificial skin from newborn skin explants is used to wrap the wound until the patient's skin is cultured and artificial skin is gained for grafting.

The production of artificial skin is following   and this is particularly cell and not organ culture. The bulk (Ca 90%) of epidermis is constituted by cells that called keratinocytes which generated the dead cells (corneocytes) making up the outermost cornfield layers of skin. The keratinocytes are dissociated by treating the skin explants with trypsin. These cells are sophisticated in vessels the bottom of which is enclosed with irradiated 3T3 fibroblast cell line; this is because certain products from fibroblast cells facilitate the proliferation of keratinocytes. Keratinocytes develop to make colonies, which are again dissociated into single cells and cultured in the same manner. The process is repeated again and again till a confluent sheet of pure epithelium is completed; this sheet is detached from the culture vessels, cleaned and used for grafting. The explants for developing artificial skin for the graft might come from the patient himself to avoid rejection. A 3 cm2 skin explants may yield about 1.7 m2 artificial skin in 3-4 weeks representing a 5000-fold increase. In approximate 5 years after grafting of the artificial skin, all the essential components of skin are regenerated. Artificial skin grafts have been used to successfully repair several types of skin defects, including chronic skin ulcers.


Related Discussions:- Artificial skin

What are the phases to atherosclerosis, Q. What are the phases to atheroscl...

Q. What are the phases to atherosclerosis? There are five phases to atherosclerosis: Phase I: Asymptomaticphase, consists of fatly streaks which are non-obstructive, lipi

Silent myocardial ischaemia, Q. Silent Myocardial Ischaemia? Ans. ...

Q. Silent Myocardial Ischaemia? Ans. This deals primarily with those who have never had symptoms recognized as being of cardiac origin. Others, who have had recognized myo

Excretory system of animals and human urinary system, steps in urine format...

steps in urine formation and the organs/tissues involve in such process?

What is mass transportation across the cell membrane, What is mass transpor...

What is mass transportation across the cell membrane? Mass transportation is the entrance or the exiting of substances in or from the cell engulfed by portions of membrane. The

Into which phyla is the fungi kingdom divided, Fungi are classified in thei...

Fungi are classified in their own kingdom. Into which phyla is the fungi kingdom divided? Into which of those phyla are mushrooms classified? The kingdom fungi is separated in

Define the sun and solar drying method, Define the Sun and Solar Drying Met...

Define the Sun and Solar Drying Method? The make use of solar energy to preserve the food items is mainly the oldest form of food reservation and is still practiced today for f

Calculate the electric potential at the midpoint of the base, The three cha...

The three charges in the figure below are at the vertices of an isosceles triangle. Let q = 7.00 nC and calculate the electric potential at the midpoint of the base.

Influence of diabetes mellitus, Influence of Diabetes Mellitus :  Diabetes...

Influence of Diabetes Mellitus :  Diabetes causes micro vascular obstructive disease in heart, kidney, retina and peripheral nerves. Scrupulous rather than casual control of diabe

Morula in humans, Which one of the following statements about morula in hum...

Which one of the following statements about morula in humans is correct? 1. It has almost equal quantity of cytoplasm as an uncleaved zygote but much more DNA 2. It has far l

Define absorption, Define Absorption, Storage and Elimination of ascorbic a...

Define Absorption, Storage and Elimination of ascorbic acid? Ascorbic acid is rapidly absorbed from the intestines primarily by active transport.  Simple diffusion or carrier-m

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd