Artificial skin, Biology

Assignment Help:

Artificial Skin:

The skin generated in vitro is in fact the epidermis portion of skin only; when the epidermis is applied to the burnt part, this leads to the regeneration of dermis (remaining parts of skin) beneath. But improvements in the approach  have allowed the reconstitution of virtually complete skin (both epidermis and dermis), called as living skin equivalent (LSE); this technology uses a collagen matrix as a support for development of the tissue. The skin explants used for attaining artificial skin may be either obtained from the patient concerned or from the foreskin (loose skin from the tip of penis) of newborn. Skin cells of new-borns grow more strongly than adult skin; the use of a synthetic polymer called as PGA helps the newborn skin to develop with no scars. Artificial skin from newborn skin explants is used to wrap the wound until the patient's skin is cultured and artificial skin is gained for grafting.

The production of artificial skin is following   and this is particularly cell and not organ culture. The bulk (Ca 90%) of epidermis is constituted by cells that called keratinocytes which generated the dead cells (corneocytes) making up the outermost cornfield layers of skin. The keratinocytes are dissociated by treating the skin explants with trypsin. These cells are sophisticated in vessels the bottom of which is enclosed with irradiated 3T3 fibroblast cell line; this is because certain products from fibroblast cells facilitate the proliferation of keratinocytes. Keratinocytes develop to make colonies, which are again dissociated into single cells and cultured in the same manner. The process is repeated again and again till a confluent sheet of pure epithelium is completed; this sheet is detached from the culture vessels, cleaned and used for grafting. The explants for developing artificial skin for the graft might come from the patient himself to avoid rejection. A 3 cm2 skin explants may yield about 1.7 m2 artificial skin in 3-4 weeks representing a 5000-fold increase. In approximate 5 years after grafting of the artificial skin, all the essential components of skin are regenerated. Artificial skin grafts have been used to successfully repair several types of skin defects, including chronic skin ulcers.


Related Discussions:- Artificial skin

Explain the term bleeding, Bleeding Bleeding from injury to small blood...

Bleeding Bleeding from injury to small blood vessels pushed along the lateral wall of expanding maxillary sinus should be contained by bone wax or electrocautery rather than cr

Developmental biology, pioneers of developmental biology and their contribu...

pioneers of developmental biology and their contribution

Causes of reduced serum hdl levels, Q. Causes of reduced Serum HDL levels? ...

Q. Causes of reduced Serum HDL levels? Possible causes of reduced Serum HDL levels: - Cigarette smoking - Hypertriglyceridemia - Obesity - Genetic factors - Lack

Sickle Cell, Sickling occurs in deoxyhemoglobin S, but not in oxyhemoglobin...

Sickling occurs in deoxyhemoglobin S, but not in oxyhemoglobin S. Oxyhemoglobin has a small hydrophobic \"pocket\" in a ß chain region located in the interior of the protein. In de

Digestion of carbohydrates, Digestion of carbohydrates Carbohydrate di...

Digestion of carbohydrates Carbohydrate digestion in vertebrates and invertebrates is very similar. All the enzymes shown in Table are not required by all animals. The enzymes

What are the chemical substances formed by water photolysis, What are the c...

What are the chemical substances formed by water photolysis? What is the destination of each of those substances? Free electrons, hydrogen ions and molecular oxygen are liberat

Concept of digestion in the stomach, Digestion in the Stomach To unders...

Digestion in the Stomach To understand  the digestion mechanism  in the stomach, it is important to know about the anatomy of the stomach. Look up Unit 6, in the Applied Physio

Polychaetes - feeding and digestion in annelids, Polychaetes - Feeding and ...

Polychaetes - Feeding and Digestion in Annelids Polychaetes involve both free moving (errant) and sedentary species. The free moving species are usually macrophagous and the s

Explain the nutritional and functional role of sulphur, Minerals :-  Sulfur...

Minerals :-  Sulfur Food Source       Widely distributed  Nutritional Functional role Essential nutrient: A constituent of the essential amino acids methionine and

General procedure of titration - nutritional biochemistry, Define General ...

Define General procedure of titration - Nutritional  Biochemistry? In general a titration requires that we add precise amounts of the known titrant to the unknown analyte or ti

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd