Artificial skin, Biology

Assignment Help:

Artificial Skin:

The skin generated in vitro is in fact the epidermis portion of skin only; when the epidermis is applied to the burnt part, this leads to the regeneration of dermis (remaining parts of skin) beneath. But improvements in the approach  have allowed the reconstitution of virtually complete skin (both epidermis and dermis), called as living skin equivalent (LSE); this technology uses a collagen matrix as a support for development of the tissue. The skin explants used for attaining artificial skin may be either obtained from the patient concerned or from the foreskin (loose skin from the tip of penis) of newborn. Skin cells of new-borns grow more strongly than adult skin; the use of a synthetic polymer called as PGA helps the newborn skin to develop with no scars. Artificial skin from newborn skin explants is used to wrap the wound until the patient's skin is cultured and artificial skin is gained for grafting.

The production of artificial skin is following   and this is particularly cell and not organ culture. The bulk (Ca 90%) of epidermis is constituted by cells that called keratinocytes which generated the dead cells (corneocytes) making up the outermost cornfield layers of skin. The keratinocytes are dissociated by treating the skin explants with trypsin. These cells are sophisticated in vessels the bottom of which is enclosed with irradiated 3T3 fibroblast cell line; this is because certain products from fibroblast cells facilitate the proliferation of keratinocytes. Keratinocytes develop to make colonies, which are again dissociated into single cells and cultured in the same manner. The process is repeated again and again till a confluent sheet of pure epithelium is completed; this sheet is detached from the culture vessels, cleaned and used for grafting. The explants for developing artificial skin for the graft might come from the patient himself to avoid rejection. A 3 cm2 skin explants may yield about 1.7 m2 artificial skin in 3-4 weeks representing a 5000-fold increase. In approximate 5 years after grafting of the artificial skin, all the essential components of skin are regenerated. Artificial skin grafts have been used to successfully repair several types of skin defects, including chronic skin ulcers.


Related Discussions:- Artificial skin

Explain foaming properties of proteins, Explain Foaming Properties of prote...

Explain Foaming Properties of proteins To understand the foaming properties of proteins, we need to know some basic aspects of foam foods. Foam foods are usually colloidal disp

How are human voices created, How are human voices created? Human voice...

How are human voices created? Human voice is formed with the help of muscles in the neck and the vocal cords. The tighter the vocal cords the higher the pitch of voice. This is

Digestion in the intestine, Digestion in the Intestine About  2  to  3 ...

Digestion in the Intestine About  2  to  3  liters  of  alkaline  fluid  is secreted every  day  by  the intestine  and completes the digestive process.  Sodium bicarbonate and

Myocardial ischemia, In patients with coronary artery disease an acute coro...

In patients with coronary artery disease an acute coronary syndrome event can precipitate heart failure. Mitral regurgitation occurring as a result of papillary muscle ischemia con

Describe the advantages of sulphur, Describe the advantages of sulphur ...

Describe the advantages of sulphur Sulphur also increases the oil content of crops such as flax and soyabeans. Disulphide linkages ( -S-S-) have recently been associated with

Enzyme vocabulary, what is a limited resourse needed by all cells?

what is a limited resourse needed by all cells?

Define about ecological models, Why do ecological models commonly have limi...

Why do ecological models commonly have limited applications? The applications of ecological models are limited due to the models are normally very simple and do not accurately

Psilopsida, why psilopsida have rootless sporophyte

why psilopsida have rootless sporophyte

Major types of inheritances exceptions to mendel''s rules/, According to th...

According to the Mendel's law the phenotypical characteristics would be determined by pair of factors (alleles) that separate independently in gametes. What are the major types of

Define body weight as a determinant of nutrient requirements, Define Body w...

Define Body weight as a determinant of nutrient requirements? Requirements are considered to be a function of body weight for individuals who are not overweight. However, for s

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd