Artificial skin, Biology

Assignment Help:

Artificial Skin:

The skin generated in vitro is in fact the epidermis portion of skin only; when the epidermis is applied to the burnt part, this leads to the regeneration of dermis (remaining parts of skin) beneath. But improvements in the approach  have allowed the reconstitution of virtually complete skin (both epidermis and dermis), called as living skin equivalent (LSE); this technology uses a collagen matrix as a support for development of the tissue. The skin explants used for attaining artificial skin may be either obtained from the patient concerned or from the foreskin (loose skin from the tip of penis) of newborn. Skin cells of new-borns grow more strongly than adult skin; the use of a synthetic polymer called as PGA helps the newborn skin to develop with no scars. Artificial skin from newborn skin explants is used to wrap the wound until the patient's skin is cultured and artificial skin is gained for grafting.

The production of artificial skin is following   and this is particularly cell and not organ culture. The bulk (Ca 90%) of epidermis is constituted by cells that called keratinocytes which generated the dead cells (corneocytes) making up the outermost cornfield layers of skin. The keratinocytes are dissociated by treating the skin explants with trypsin. These cells are sophisticated in vessels the bottom of which is enclosed with irradiated 3T3 fibroblast cell line; this is because certain products from fibroblast cells facilitate the proliferation of keratinocytes. Keratinocytes develop to make colonies, which are again dissociated into single cells and cultured in the same manner. The process is repeated again and again till a confluent sheet of pure epithelium is completed; this sheet is detached from the culture vessels, cleaned and used for grafting. The explants for developing artificial skin for the graft might come from the patient himself to avoid rejection. A 3 cm2 skin explants may yield about 1.7 m2 artificial skin in 3-4 weeks representing a 5000-fold increase. In approximate 5 years after grafting of the artificial skin, all the essential components of skin are regenerated. Artificial skin grafts have been used to successfully repair several types of skin defects, including chronic skin ulcers.


Related Discussions:- Artificial skin

Sticky sweet method-endodontics principles, Sticky sweet method Richwi...

Sticky sweet method Richwil crown and bridge remover -It is a resin block of water-soluble , like a stick sweet -It is first softened in hot water -Placed on the restorat

Is calcium-sensing receptors serves as a sensor, Which of the following ser...

Which of the following serves as a sensor, or as part of a sensor, that functions in a positive feedback system? A. CaSRs (Calcium-Sensing Receptors) situated in the plasma mem

How parasitic infections are found, How Parasitic infections are found? ...

How Parasitic infections are found? Parasitic infections are found throughout the world. With increasing travel, immigration, use of immunosuppressive drugs and the spread of A

Describe the types of drugs, Describe the types of Drugs • Induction a...

Describe the types of Drugs • Induction agent (thiopentone, fentanyl, ketamine, midazolam) • Suxamethonium (1-2 mg/kg) is the muscle relaxant of choice. Rapid sequence ind

Define briefly about the cereal proteins, Define briefly about the Cereal P...

Define briefly about the Cereal Proteins? Cereal grains, properly ripened and dried for optimum storage stability, have protein content ranging from 6-20%. Proteins are found i

What are dimorphic fungi, Question 1 What are dimorphic fungi? List the cl...

Question 1 What are dimorphic fungi? List the clinical manifestations produced by various dimorphic fungi. Add a note on isolation and identification of various dimorphic fungi

State about the neuropsychological assessment, State about the Neuropsychol...

State about the Neuropsychological assessment Neuropsychological assessment provides information concerning the status of brain function across the life span. It does so primar

Palpation , Palpation: Use the ball of one hand  and move the hand  from ...

Palpation: Use the ball of one hand  and move the hand  from one side of the posterior thorax to other. Note presence of swelling, masses, muscle tone, fremitus and crepitation.

How to evaluate blanching efficiency, How to evaluate blanching efficiency?...

How to evaluate blanching efficiency? Finally, let us learn how to evaluate blanching efficiency? Normally, two of the more heat resistant plant enzymes, namely peroxidase and

Class of crustacea - malacostraca, Class of Crustacea - Malacostraca M...

Class of Crustacea - Malacostraca Malacostraca involves most of the larger forms such as crabs, lobsters, shrimps etc. and constitute the majority of crustacean species. The c

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd