Artificial skin, Biology

Assignment Help:

Artificial Skin:

The skin generated in vitro is in fact the epidermis portion of skin only; when the epidermis is applied to the burnt part, this leads to the regeneration of dermis (remaining parts of skin) beneath. But improvements in the approach  have allowed the reconstitution of virtually complete skin (both epidermis and dermis), called as living skin equivalent (LSE); this technology uses a collagen matrix as a support for development of the tissue. The skin explants used for attaining artificial skin may be either obtained from the patient concerned or from the foreskin (loose skin from the tip of penis) of newborn. Skin cells of new-borns grow more strongly than adult skin; the use of a synthetic polymer called as PGA helps the newborn skin to develop with no scars. Artificial skin from newborn skin explants is used to wrap the wound until the patient's skin is cultured and artificial skin is gained for grafting.

The production of artificial skin is following   and this is particularly cell and not organ culture. The bulk (Ca 90%) of epidermis is constituted by cells that called keratinocytes which generated the dead cells (corneocytes) making up the outermost cornfield layers of skin. The keratinocytes are dissociated by treating the skin explants with trypsin. These cells are sophisticated in vessels the bottom of which is enclosed with irradiated 3T3 fibroblast cell line; this is because certain products from fibroblast cells facilitate the proliferation of keratinocytes. Keratinocytes develop to make colonies, which are again dissociated into single cells and cultured in the same manner. The process is repeated again and again till a confluent sheet of pure epithelium is completed; this sheet is detached from the culture vessels, cleaned and used for grafting. The explants for developing artificial skin for the graft might come from the patient himself to avoid rejection. A 3 cm2 skin explants may yield about 1.7 m2 artificial skin in 3-4 weeks representing a 5000-fold increase. In approximate 5 years after grafting of the artificial skin, all the essential components of skin are regenerated. Artificial skin grafts have been used to successfully repair several types of skin defects, including chronic skin ulcers.


Related Discussions:- Artificial skin

Preliminary screening stage of battery, Preliminary screening stage of batt...

Preliminary screening stage of battery During the preliminary screening stage in the development of the battery, items in the original pool that did not attain satisfactory int

Liquid manure handling, L iq uid manure handling This involves use of...

L iq uid manure handling This involves use of lot of water to flush the manure in to collection tank. Storage capacity depends on type of farm, irrigation facility, storage t

Discuss about smooth muscles, Smooth muscles These muscles are beyond o...

Smooth muscles These muscles are beyond our control. That means these muscles are controlled by autonomic nervous system. They are non-voluntary muscles. They are found in thin

How do viruses enter plant body, a) How do viruses enter plant body and spr...

a) How do viruses enter plant body and spread to long distances within it? b) Mention any two ways by which viruses spread from single plant to another.

Anticodon, Anticodon is the series of three nucleotides on the transfer RN...

Anticodon is the series of three nucleotides on the transfer RNA molecule which recognizes and pairs with the specific codon on a messenger RNA molecule; it helps in controlling t

Explain the decolourizing agent - stain technique, Explain the Decolourizin...

Explain the Decolourizing Agent - Stain Technique? 95% ethanol is used as a decolourizing agent. It has two functions - (1) It acts as protein - dehydrating agent, and (2

Is it possible x chromosome of women come from father, Is it possible that ...

Is it possible that an X chromosome of a woman can have come from her father? It isn't only possible that an X chromosome of a woman is from her father, it is certain. Each wom

What is the parasite that causes giardiasis, Q. What is the parasite that c...

Q. What is the parasite that causes giardiasis? How is it transmitted and what are the typical manifestations of the disease? The Giardiasis is a protozoal infection caused by

What are the difference between plasma membrane, Q. What are the difference...

Q. What are the difference between plasma membrane and cell wall? Plasma membrane and cell wall is not the same thing. Plasma membrane, also known as cell membrane, is the exte

Transcribed regions, Transcribed regions The transcribed regions of the...

Transcribed regions The transcribed regions of the genes contain a number of regulatory elements that control exon splicing, mRNA transfer to cytoplasm translation, mRNA target

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd