Artificial skin, Biology

Assignment Help:

Artificial Skin:

The skin generated in vitro is in fact the epidermis portion of skin only; when the epidermis is applied to the burnt part, this leads to the regeneration of dermis (remaining parts of skin) beneath. But improvements in the approach  have allowed the reconstitution of virtually complete skin (both epidermis and dermis), called as living skin equivalent (LSE); this technology uses a collagen matrix as a support for development of the tissue. The skin explants used for attaining artificial skin may be either obtained from the patient concerned or from the foreskin (loose skin from the tip of penis) of newborn. Skin cells of new-borns grow more strongly than adult skin; the use of a synthetic polymer called as PGA helps the newborn skin to develop with no scars. Artificial skin from newborn skin explants is used to wrap the wound until the patient's skin is cultured and artificial skin is gained for grafting.

The production of artificial skin is following   and this is particularly cell and not organ culture. The bulk (Ca 90%) of epidermis is constituted by cells that called keratinocytes which generated the dead cells (corneocytes) making up the outermost cornfield layers of skin. The keratinocytes are dissociated by treating the skin explants with trypsin. These cells are sophisticated in vessels the bottom of which is enclosed with irradiated 3T3 fibroblast cell line; this is because certain products from fibroblast cells facilitate the proliferation of keratinocytes. Keratinocytes develop to make colonies, which are again dissociated into single cells and cultured in the same manner. The process is repeated again and again till a confluent sheet of pure epithelium is completed; this sheet is detached from the culture vessels, cleaned and used for grafting. The explants for developing artificial skin for the graft might come from the patient himself to avoid rejection. A 3 cm2 skin explants may yield about 1.7 m2 artificial skin in 3-4 weeks representing a 5000-fold increase. In approximate 5 years after grafting of the artificial skin, all the essential components of skin are regenerated. Artificial skin grafts have been used to successfully repair several types of skin defects, including chronic skin ulcers.


Related Discussions:- Artificial skin

Electro magnetic radiation, Electro magnetic radiation Light is one ...

Electro magnetic radiation Light is one form of energy. The light rays consist of tiny particles called photons. The energy carried by photon is called quantum. Light

Sexual reproduction in animals, Sexual Reproduction All metazoans, if...

Sexual Reproduction All metazoans, if or if not capable of propagating asexually, produce special cells termed as gametes. The gametes may be either male gametes or sperms or

Explain the term tracheostomy, Explain the term Tracheostomy ? If the d...

Explain the term Tracheostomy ? If the duration of mechanical ventilation is likely to be prolonged, it is wise to do an early tracheostomy. Otherwise, a tracheostomy is usuall

Traditional uses of dung, T r ad i tional Uses of dung Dung has an ...

T r ad i tional Uses of dung Dung has an astonishing and myriad variety of uses which have been developed over a period of time. Dung patties ( gootee ) are mainly used by

Animal biotechnology, Animal Biotechnology: Animal cell cultures have b...

Animal Biotechnology: Animal cell cultures have been and are being generate important products based on their genetic information or due to genes transferred into them (transge

Problem of potency of blastema cells, Problem of Potency of Blastema Cells ...

Problem of Potency of Blastema Cells The local cells are accountable for regeneration. Now you could ask which of the blastema cells differentiated into which type of limb ti

Define species of biological species concept, Define species according to t...

Define species according to the biological species concept. What two problems often make this definition impractical?

Explain haart, Patients Not on HAART For HIV-infected patients requiri...

Patients Not on HAART For HIV-infected patients requiring TB treatment who are not currently being treated with highly active antiretroviral therapy (HAART), it may be prudent

draw the cell at metaphase, Consider cell with a diploid chromosome number...

Consider cell with a diploid chromosome number of 4 (2n=4), one pair of the chromosomes is metacentric,the other is acrocentric. The metacentric is labeled a 1 a 1 while the acr

Acetylcholine, Acetylcholine is the chemical released at neuromuscular jun...

Acetylcholine is the chemical released at neuromuscular junctions which binds to receptors on the surface of the plasma membrane of the muscle cells, creating an electrical impuls

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd