Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Antibodies
Antibodies are important.tools for detecting and localising specific molecules in the cells due to their high specificity. The first requirement for this is to produce antibodies against a specific molecule. Suppose you want to produce antibodies against tubulin, the purified tubulin is first obtained from a given tissue. It is then injected into an animal such as rabbit which in nwse to tubulin produces antibodies. Blood sample is collected from the animal and antibodies are isolated. These antibodies are used to locakise the specific molecule in the cell because they bind only 80 an antigen, which is a tubulia in this case. Localisation can be done by two methods: direct and inditect In the direct method, antibodies are first labelled with a flouroscendt dye-like rhodamide for light microscopy, or witha high molecular weight, electron dense compound like ferritin for transmission electron microscopy. Fluorescent labelled antibodies are allowed to react with cells so as to form systematic antibody complex. The complex is then examined undera microscope (fluorescent microscope or TEM). The specific molecules will emit light or become electron dense due to their binding with labelled antibodies (Figure shown below). This method is used to locdise foreign bodies such us viruses, bacterial antigevs, etc.
What are the uses of whey protein concentrates? Whey protein concentrates are used extensively in the manufacture of baked goods, where they increase the effectiveness of short
Define interaction of Vitamin C with Riboflavin? The two coenzyme forms of riboflavin are FMN and FAD. They are found in a large number of systems which function in the metabol
Protection of human health should be the primary consideration in risk management decisions. Decisions on acceptable levels of risk should be determined primarily by hum
From which germ layer do the epidermis and the nervous system originate? What are other organs and tissues made from that germ layer? Epidermis and nervous system have the simi
Pathphysiology Streptococcal upper respiratory infection leads to production of antibodies. An abnormal immunological response to the upper respiratory infection with Beta- h
Q. Prevention and Control from escherichia coli? Prevention and Control: Involves avoiding contaminated food and water that have high coliform counts, avoiding unpasteurized ju
Q. Do the amine and the carboxyl groups attached to central carbons participate in the union between amino acids? Yes. The nitrogen of the amine group of one amino acid binds t
Define the Iodine Test - Carbohydrates? This test is given by polysaccharides and is not given by monosaccharides or disaccharides. Principle Iodine forms a coloured ad
Process of Regeneration in Planarians Regeneration in planarians has been broadly studied and considerable information regarding this phenomenon is now available. Let us see w
Pollinia and Massulae In most cases the pollen grains of each tetrad become separated from one another and they lie freely in the pollen sac. In some plants, mostly members of
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd