Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Antibodies
Antibodies are important.tools for detecting and localising specific molecules in the cells due to their high specificity. The first requirement for this is to produce antibodies against a specific molecule. Suppose you want to produce antibodies against tubulin, the purified tubulin is first obtained from a given tissue. It is then injected into an animal such as rabbit which in nwse to tubulin produces antibodies. Blood sample is collected from the animal and antibodies are isolated. These antibodies are used to locakise the specific molecule in the cell because they bind only 80 an antigen, which is a tubulia in this case. Localisation can be done by two methods: direct and inditect In the direct method, antibodies are first labelled with a flouroscendt dye-like rhodamide for light microscopy, or witha high molecular weight, electron dense compound like ferritin for transmission electron microscopy. Fluorescent labelled antibodies are allowed to react with cells so as to form systematic antibody complex. The complex is then examined undera microscope (fluorescent microscope or TEM). The specific molecules will emit light or become electron dense due to their binding with labelled antibodies (Figure shown below). This method is used to locdise foreign bodies such us viruses, bacterial antigevs, etc.
Epidemiology The organism does not appear to be very host or tissue specific and can infect naturally a large number of avian and mammalian species. Chlamydiosis has been reco
Reparative Regeneration This type of regeneration, as the name suggests, involves repair of a wound or replacement of a body part removed intentionally or because of injury.
Delimitation and Limitation: There may be many aspects of the problem that need to be explored, but it is difficult to cover all aspects in a single research study bec
Effect of Temperature Certain plants such as the winter rye (Secale cereale) and the biennial strain of henbane (Hyoscyamus Niger) require exposure to low temperature conditio
How is water's importance to life reflected in the normal range of internal pH values that living things are likely to have?
Ribonucleases and deoxyribonucleases These enzymes are responsible for the degradation of dietary nucleic acids. Ribonucleases and deoxyribonucleases secreted by the
Q. Define Premotor Cortex Located in the frontal lobes? Premotor Cortex - Involved in control of more complex, learned motor skills like writing, driving, etc. Broca's area or
Q. Illustrate Dilated cardiomyopathy? It is a disease of unknown etiology, affecting myocardium. Its diagnosis is established by presence of left ventricular dilatation and sys
Q. What are the main risk factors for hypertension? The major risks factors for hypertension are tobacco smoking, stress, obesity, sedentary lifestyle and alcoholism.
What are the elements that constitute the stomata? The Stomata is made of a central opening the ostiole or slit delimited by two guard cells responsible for its closing or open
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd