Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Antibodies
Antibodies are important.tools for detecting and localising specific molecules in the cells due to their high specificity. The first requirement for this is to produce antibodies against a specific molecule. Suppose you want to produce antibodies against tubulin, the purified tubulin is first obtained from a given tissue. It is then injected into an animal such as rabbit which in nwse to tubulin produces antibodies. Blood sample is collected from the animal and antibodies are isolated. These antibodies are used to locakise the specific molecule in the cell because they bind only 80 an antigen, which is a tubulia in this case. Localisation can be done by two methods: direct and inditect In the direct method, antibodies are first labelled with a flouroscendt dye-like rhodamide for light microscopy, or witha high molecular weight, electron dense compound like ferritin for transmission electron microscopy. Fluorescent labelled antibodies are allowed to react with cells so as to form systematic antibody complex. The complex is then examined undera microscope (fluorescent microscope or TEM). The specific molecules will emit light or become electron dense due to their binding with labelled antibodies (Figure shown below). This method is used to locdise foreign bodies such us viruses, bacterial antigevs, etc.
Q. What do you mean by Cytotaxonomu and biosustematics? Towards the end of the 19 th century and in the early years of the 20th century, botanists were faced with a problem of
Why is the determination of the blood types of the donor and of the recipient important in transfusions? Red blood cells have dissimilar antigens in the outer surface of their
Q What are the three major types of nitrogen wastes excreted by living beings? The main nitrogen wastes excreted by living beings are ammonia, urea and uric acid. Living beings
Ask question #Minimum 100 hhejejhjehjwords accepted#
Q. What are the major orders of placental mammals? What are a few representative species and distinguishing features of each of those orders? The orders into which placental ma
Estrus - Estrous cycle This is the period of heat, and copulation is permitted only at this time. This condition lasts from 9 to 15 hours and is characterised by a high rate o
Define Absorption, transport and Excretion of phosphorus? As you have seen that food contains both organic and inorganic phosphorus, but most of it is absorbed in its inorganic
What are the two main classes into which fishes are divided? Fishes are separated into two main classes: chondrichthyes, fishes with cartilaginous skeletons (sharks, rays, dog-
Explain about the Food product development? Food product development is often commodity related. This type of research needs to be carried out in the pilot plants with the equi
Is the nervous system in platyhelminthes more or less sophisticated than in cnidarians? What are the main neural structures found in flatworms? How is this neural organization impo
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd