Antibodies, Biology

Assignment Help:

Antibodies

Antibodies are important.tools for detecting and localising specific molecules in the cells due to their high specificity. The first requirement for this is to produce antibodies against a specific molecule. Suppose you want to produce antibodies against tubulin, the purified tubulin is first obtained from a given tissue. It is then injected into an animal such as rabbit which in nwse to tubulin produces antibodies. Blood sample is collected from the animal and antibodies are isolated. These antibodies are used to locakise the specific molecule in the cell because they bind only 80 an antigen, which is a tubulia in this case. Localisation can be done by two methods: direct and inditect

In the direct method, antibodies are first labelled with a flouroscendt dye-like rhodamide for light microscopy, or witha high molecular weight, electron dense compound like ferritin for transmission electron microscopy. Fluorescent labelled antibodies are allowed to react with cells so as to form systematic antibody complex. The complex is then examined under
a microscope (fluorescent microscope or TEM). The specific molecules will emit light or become electron dense due to their binding with labelled antibodies (Figure shown below). This method is used to locdise foreign bodies such us viruses, bacterial antigevs, etc.

618_antibodies.jpg


Related Discussions:- Antibodies

Photosynthesis - responses of plants to stress, Photosynthesis - Responses ...

Photosynthesis - Responses of Plants to Stress Photosynthesis is also inhibited due to low water potential in the leaves. The leaf water potential is the sum of turgor pressur

Carrier protein in a plasma membrane, What is a characteristic feature of a...

What is a characteristic feature of a carrier protein in a plasma membrane? Carrier proteins are globular proteins which are particular it their action and therefore regulate t

Haccp control measure, HACCP Control Measure HACCP Control Measure : ...

HACCP Control Measure HACCP Control Measure :  Any action or activity that can be used to prevent, eliminate or reduce a significant hazard.

Types of retaining structure, What are the types of evaluation of lateral e...

What are the types of evaluation of lateral earth forces acting on retaining structures? This section seeks to introduce you to the evaluation of lateral earth forces acting on

What is peaked t-waves, Q. What is Peaked T-Waves? Exercise induced inc...

Q. What is Peaked T-Waves? Exercise induced increase in T-wave amplitude in V2 is quite predictive of anterior wall ischaemia (specificity = 95 percent). It appears that this m

Radiobiology, Radiobiology : This is the study of effects of radiations on ...

Radiobiology : This is the study of effects of radiations on living organisms. Radiobiology is a branch of science which concerned with the action of ions. Radiobiology is the radi

Procedures for diagnosis - immune responses and biomarkers, Demonstration o...

Demonstration of immune responses and biomarkers: This may be done either on the basis of sero-conversion study on paired sera samples or by challenge (protection) test.

Protazoa, In what order did the four phyla of protazoa evolve and why in th...

In what order did the four phyla of protazoa evolve and why in this order?

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd