Antibodies, Biology

Assignment Help:

Antibodies

Antibodies are important.tools for detecting and localising specific molecules in the cells due to their high specificity. The first requirement for this is to produce antibodies against a specific molecule. Suppose you want to produce antibodies against tubulin, the purified tubulin is first obtained from a given tissue. It is then injected into an animal such as rabbit which in nwse to tubulin produces antibodies. Blood sample is collected from the animal and antibodies are isolated. These antibodies are used to locakise the specific molecule in the cell because they bind only 80 an antigen, which is a tubulia in this case. Localisation can be done by two methods: direct and inditect

In the direct method, antibodies are first labelled with a flouroscendt dye-like rhodamide for light microscopy, or witha high molecular weight, electron dense compound like ferritin for transmission electron microscopy. Fluorescent labelled antibodies are allowed to react with cells so as to form systematic antibody complex. The complex is then examined under
a microscope (fluorescent microscope or TEM). The specific molecules will emit light or become electron dense due to their binding with labelled antibodies (Figure shown below). This method is used to locdise foreign bodies such us viruses, bacterial antigevs, etc.

618_antibodies.jpg


Related Discussions:- Antibodies

Describe methods of diagnosing chromosome abnormalities, Explain the differ...

Explain the difference between numerical chromosome abnormalities and single gene disorders. Describe methods of diagnosing chromosome abnormalities prenatally and after birth. How

Races of modern man, RACE S OF MODERN MAN Australoids Negroids ...

RACE S OF MODERN MAN Australoids Negroids Caucasoids Monogoloids American Indian Polynesian All human races have the same number of chromosomes (46) and b

Fats requirements during congestive cardiac failure, Q. Fats requirements d...

Q. Fats requirements during congestive cardiac failure? Fat: The quantity and quality of fat would be governed by the severity of hyper- lipidemia and adiposity. Emphasis, as

Define water output (losses of body water) - renal loss, Define Water Outpu...

Define Water Output (Losses of body water) - Renal loss? Water is lost from the body by the four routes, namely kidneys (renal system), skin, lungs and intestine. Let us discus

State the term - aneurysms, State the term - Aneurysms Aneurysms are va...

State the term - Aneurysms Aneurysms are vascular dilations resulting from localised defects in the elasticity of a vessel. They can be visualised as balloon like expansions of

Absorption of food - zoology, ABSORPTIO N OF FOOD Absorption from Mo...

ABSORPTIO N OF FOOD Absorption from Mouth Here negligible tobacco, alkohal and some medicines as isoprenailne glycerol tri nitrite is absorbed. Absorptio n from Sto

Explain the concepts of food chain and food web, What is the difference bet...

What is the difference between the concepts of food chain and food web? The chain method is a theoretical model to study the energy flux in ecosystems. Actually in an ecosystem

Explain insect resistant crops, Insect resistant crops (IRC) Should...

Insect resistant crops (IRC) Should reduce use of insecticides. This is beneficial to environment because: more beneficial insects around as they are not killed by ins

Chicken infectious anemia (cia), C h ic k e n infectious anemia (CIA) ...

C h ic k e n infectious anemia (CIA) Chicken infectious anemia virus (CIAV), a member of genus Gyrovirus of the family Circoviridae, is the causative agent of chicken infe

Define some precautions for measurement of ph, Define some Precautions for ...

Define some Precautions for Measurement of pH? 1. Never touch the membrane of the glass electrode with anything else except soft tissue paper since it is fragile and is easily

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd