Antibodies, Biology

Assignment Help:

Antibodies

Antibodies are important.tools for detecting and localising specific molecules in the cells due to their high specificity. The first requirement for this is to produce antibodies against a specific molecule. Suppose you want to produce antibodies against tubulin, the purified tubulin is first obtained from a given tissue. It is then injected into an animal such as rabbit which in nwse to tubulin produces antibodies. Blood sample is collected from the animal and antibodies are isolated. These antibodies are used to locakise the specific molecule in the cell because they bind only 80 an antigen, which is a tubulia in this case. Localisation can be done by two methods: direct and inditect

In the direct method, antibodies are first labelled with a flouroscendt dye-like rhodamide for light microscopy, or witha high molecular weight, electron dense compound like ferritin for transmission electron microscopy. Fluorescent labelled antibodies are allowed to react with cells so as to form systematic antibody complex. The complex is then examined under
a microscope (fluorescent microscope or TEM). The specific molecules will emit light or become electron dense due to their binding with labelled antibodies (Figure shown below). This method is used to locdise foreign bodies such us viruses, bacterial antigevs, etc.

618_antibodies.jpg


Related Discussions:- Antibodies

Define the symptoms in bone marrow of pernicious anaemia, Define the sympto...

Define the symptoms in Bone marrow of pernicious anaemia? The nucleated red cells of the marrow are greatly increased.  The successive nucleated cell stages in erythropoiesis a

Energy transformation, ENERG Y TRANSFORMATION - During photosynthes...

ENERG Y TRANSFORMATION - During photosynthesis radiant energy is converted into chemical energy by green plants. In biluminiscent organisms .e.g glow worm, noctilluca &

What is a stream, What is a stream? A stream is a incessant series of by...

What is a stream? A stream is a incessant series of bytes that flow into or out of your program. Input and output from devices like the mouse, , disk, keyboard, screen, modem, a

How to identify the monomer or molecular components, Please help with the f...

Please help with the following: For each of the four macromolecules carbs, lipids, proteins and nucleic acids, please identify the monomer or molecular components and name the b

Polytene chromosomes, PO L YTEN E CHROMOSOMES First seen by Balb...

PO L YTEN E CHROMOSOMES First seen by Balbiani in salivary glands of larva of Chironomus insect (belongs to order Diptera). Later on such chromosomes observed in severa

What do you mean by alpha taxonomy, Q. What do you mean by Alpha Taxonomy? ...

Q. What do you mean by Alpha Taxonomy? This relates to the basic or preliminary classification based almost entirely on external morphology. This can be considered as the empir

Explain starch phosphates monoesters, Explain Starch phosphates monoesters ...

Explain Starch phosphates monoesters and  Hydroxypropyl starches   Starch phosphates monoesters Emulsifying agent and thickening agent. Hydroxypropyl starches

Compound Microscope, The highest possible magnification that can obtain whe...

The highest possible magnification that can obtain when using using a microscope?

Poultry and duck diseases-fowl pox, Fowl pox Fowl-pox is a highly infec...

Fowl pox Fowl-pox is a highly infectious disease of poultry, especially chickens and turkeys. Pheasants, ducks, geese and guineafowls also suffer from an infection similar to f

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd