Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Antibodies
Antibodies are important.tools for detecting and localising specific molecules in the cells due to their high specificity. The first requirement for this is to produce antibodies against a specific molecule. Suppose you want to produce antibodies against tubulin, the purified tubulin is first obtained from a given tissue. It is then injected into an animal such as rabbit which in nwse to tubulin produces antibodies. Blood sample is collected from the animal and antibodies are isolated. These antibodies are used to locakise the specific molecule in the cell because they bind only 80 an antigen, which is a tubulia in this case. Localisation can be done by two methods: direct and inditect In the direct method, antibodies are first labelled with a flouroscendt dye-like rhodamide for light microscopy, or witha high molecular weight, electron dense compound like ferritin for transmission electron microscopy. Fluorescent labelled antibodies are allowed to react with cells so as to form systematic antibody complex. The complex is then examined undera microscope (fluorescent microscope or TEM). The specific molecules will emit light or become electron dense due to their binding with labelled antibodies (Figure shown below). This method is used to locdise foreign bodies such us viruses, bacterial antigevs, etc.
Sexual reproduction in Eukaryotes In most eukaryotes, especially higher animals, individuals normally exhibit one or two sex phenotypes; female or male. In such species, females
How Pathologic Conditions causing the underweight? Illness can affect weight status in a number of ways. For example, fevers and infections increase the demand for energy, whic
state 5 notification and feeding habits in some animals
What is a genotype? What is the difference between genotype and phenotype? Genotype is the genes, DNA nucleotide sequences have in the chromosomes of an individual that conditi
In the presence of oxygen, the process of glycolysis produces which of the following products? A) 2 glycerol B) 1 lactate C) 2 lactate D) 2 pyruvate E) 1 pyruvate
Feeding mechanism in holozoic animal
How Age affects the BMR? The BMR per unit weight also varies with age, being higher in children and lower in the elderly. The loss of FFM with ageing is associated with a decli
Artificial respiration First - aid for drowning victims should aim to restore respiration as quickly as possible. First of all, nose, mouth and throat of the victim shoul
Extracellular Aging The basic components of extracellular space are mucopolysaccharides and fibrous proteins, particularly collagen and elastin. These proteins are synthesised
A dominant allele will always increase in frequency over time. Yes No Q44. When a gene has two alleles, the allele frequencies in a population automatically move toward an equilibr
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd