Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Antibodies
Antibodies are important.tools for detecting and localising specific molecules in the cells due to their high specificity. The first requirement for this is to produce antibodies against a specific molecule. Suppose you want to produce antibodies against tubulin, the purified tubulin is first obtained from a given tissue. It is then injected into an animal such as rabbit which in nwse to tubulin produces antibodies. Blood sample is collected from the animal and antibodies are isolated. These antibodies are used to locakise the specific molecule in the cell because they bind only 80 an antigen, which is a tubulia in this case. Localisation can be done by two methods: direct and inditect In the direct method, antibodies are first labelled with a flouroscendt dye-like rhodamide for light microscopy, or witha high molecular weight, electron dense compound like ferritin for transmission electron microscopy. Fluorescent labelled antibodies are allowed to react with cells so as to form systematic antibody complex. The complex is then examined undera microscope (fluorescent microscope or TEM). The specific molecules will emit light or become electron dense due to their binding with labelled antibodies (Figure shown below). This method is used to locdise foreign bodies such us viruses, bacterial antigevs, etc.
Establishment of Tissue Cultures By now you are familiar with the term "Explants". After a few days in culture the explant becomes slightly rough in texture and its surface g
What is the Population of Ecology explain? Ecology is also studied at a higher level of organization the population level. In biological terms, a population is defined as a gro
What are the two main morphological patterns of cnidarians? Concerning locomotion how do these forms differentiate from each other? Morphologically, cnidarians categorize as po
TYPES OF DRUG ACTION - 1 . PHARMACODYNAMIC DRUGS - These drugs modify the physiological and biochemical processes in the patient's body to eliminate the disease.
Explain the term Direct Calorimetry? Calorimetry refers to the measurement of the amount of heat evolved or absorbed in a chemical reaction, change of state, or formation of a
Properties of Isozymes Isozymes differ from each other not only in the amino acid composition, they also have different electrophoretic property, thermolability, immunologi
Name the high-energy complex generated during the conversion of succinyl CoA to succinate in the citric acid cycle GTP or ATP is the high-energy complex generated during th
Q. Explain Surlyn film? Surlyn films: Surlyn (SU) is a versatile film which is sometimes favoured over polyethylene due to some unique properties such as excellent moisture b
Starch It is a plant polysaccharide synthesized by the plant by photosynthesis and stored mainly in grains, legumes, roots and tubers. Its molecular formula is (C 6 H 1
Fine Structure of Pollen Tubes Grown in Vitro The growth in pollen tubes is exclusively restricted to the tip. Cytochemical analysis reveals the pollen tip zone to be rich in
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd