Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Antibodies
Antibodies are important.tools for detecting and localising specific molecules in the cells due to their high specificity. The first requirement for this is to produce antibodies against a specific molecule. Suppose you want to produce antibodies against tubulin, the purified tubulin is first obtained from a given tissue. It is then injected into an animal such as rabbit which in nwse to tubulin produces antibodies. Blood sample is collected from the animal and antibodies are isolated. These antibodies are used to locakise the specific molecule in the cell because they bind only 80 an antigen, which is a tubulia in this case. Localisation can be done by two methods: direct and inditect In the direct method, antibodies are first labelled with a flouroscendt dye-like rhodamide for light microscopy, or witha high molecular weight, electron dense compound like ferritin for transmission electron microscopy. Fluorescent labelled antibodies are allowed to react with cells so as to form systematic antibody complex. The complex is then examined undera microscope (fluorescent microscope or TEM). The specific molecules will emit light or become electron dense due to their binding with labelled antibodies (Figure shown below). This method is used to locdise foreign bodies such us viruses, bacterial antigevs, etc.
Punnet squares
1. Discuss how nursing has changed over the last two (2) decades. 2. Review and summarise the philosophy of your enrolled nurse course and its relevance to your clinical practic
Rigs Pattern: The nursing unit has been divided into small cubicles for 1,2,4 and 6 beds. The beds are arranged parallel to the longitudinal wall. The pattern was first bu
V apour Theory (i) It was proposed by a Greek philosopher Pythagoras in 500 B.C. (ii) Each organ of an animal body emitted some kind of vapour and that a ne
Q. What is continuous full thickness incision? 2. Continuous full - thickness incisions are indicated for a variety of situations outlined below. This type of incision may be u
The scutellum observed in a grain of wheat or maize is comparable to which part of the seed in other monocotyledons? 1. Cotyledon 2. Endosperm 3. Aleurone layer 4. Plum
Brackish Water The content of dissolved salts in brackish water is higher than the fresh water and ranges between 0.5 to 35%. These waters of intermediate salinity range are d
differences between acoelomate,coelomate and pseudocoelomate
Explain the Most Probable Number (MPN) Techniques? Different procedures and media may be used depending on the food tested and the information sought. Media which can be used i
I nfe c tio u s bursal disease Infectious bursal disease (popularly known as Gumboro disease) is an acute contagious disease of young chicks caused by an RNA virus, a memb
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd