Antibodies, Biology

Assignment Help:

Antibodies

Antibodies are important.tools for detecting and localising specific molecules in the cells due to their high specificity. The first requirement for this is to produce antibodies against a specific molecule. Suppose you want to produce antibodies against tubulin, the purified tubulin is first obtained from a given tissue. It is then injected into an animal such as rabbit which in nwse to tubulin produces antibodies. Blood sample is collected from the animal and antibodies are isolated. These antibodies are used to locakise the specific molecule in the cell because they bind only 80 an antigen, which is a tubulia in this case. Localisation can be done by two methods: direct and inditect

In the direct method, antibodies are first labelled with a flouroscendt dye-like rhodamide for light microscopy, or witha high molecular weight, electron dense compound like ferritin for transmission electron microscopy. Fluorescent labelled antibodies are allowed to react with cells so as to form systematic antibody complex. The complex is then examined under
a microscope (fluorescent microscope or TEM). The specific molecules will emit light or become electron dense due to their binding with labelled antibodies (Figure shown below). This method is used to locdise foreign bodies such us viruses, bacterial antigevs, etc.

618_antibodies.jpg


Related Discussions:- Antibodies

Caryopsis - development of fruit, Caryopsis - Development of Fruit In ...

Caryopsis - Development of Fruit In cereals each carpel has one ovule and therefore the mature fruit has, just one seed. During maturation, very little or no cell divisions ar

Explain how a person can be dead, A person is declared to be dead upon the ...

A person is declared to be dead upon the irreversible cessation of spontaneous body functions; brain activity, or blood circulation and respiration. However, only about 1% of a per

Explain the transport of amino acids, Explain the Transport of Amino Acids?...

Explain the Transport of Amino Acids? More than one transport or carrier system functions in the absorption of amino acids. The active carrier system for neutral amino acids sh

How is genetic determination of sex established in humans, How is the genet...

How is the genetic determination of sex established in humans? In the diploid genome of the human beings, there are 46 chromosomes, 44 of them are autosomes and two are sex chr

What is community ecology explain their characterstics, What is Community E...

What is Community Ecology explain their characterstics? Community Ecology: In ecological terms, a community consists of an assemblage of all of the populations living and int

How is hiv transmitted, Q. How is HIV transmitted? What is the disease caus...

Q. How is HIV transmitted? What is the disease caused by this virus? The HIV (human immunodeficiency virus) is supposed to be transmitted through blood, semen, vaginal secretio

Can you explain non-restrictive ventricular septal defect, Q. Can you expla...

Q. Can you explain Non-restrictive ventricular septal defect? In non-restrictive VSD PA pressures are elevated hence VSD gradient is low. Non-restrictive VSD is larger in s

What do energy pyramids represent, Q. What do energy pyramids represent? ...

Q. What do energy pyramids represent? The Energy pyramids represent the amount of accessible energy in each trophic level of the food chain.

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd