Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Antibodies
Antibodies are important.tools for detecting and localising specific molecules in the cells due to their high specificity. The first requirement for this is to produce antibodies against a specific molecule. Suppose you want to produce antibodies against tubulin, the purified tubulin is first obtained from a given tissue. It is then injected into an animal such as rabbit which in nwse to tubulin produces antibodies. Blood sample is collected from the animal and antibodies are isolated. These antibodies are used to locakise the specific molecule in the cell because they bind only 80 an antigen, which is a tubulia in this case. Localisation can be done by two methods: direct and inditect In the direct method, antibodies are first labelled with a flouroscendt dye-like rhodamide for light microscopy, or witha high molecular weight, electron dense compound like ferritin for transmission electron microscopy. Fluorescent labelled antibodies are allowed to react with cells so as to form systematic antibody complex. The complex is then examined undera microscope (fluorescent microscope or TEM). The specific molecules will emit light or become electron dense due to their binding with labelled antibodies (Figure shown below). This method is used to locdise foreign bodies such us viruses, bacterial antigevs, etc.
Q. How do the potassium and sodium ions maintain the resting potential of the neuron? The plasma membrane of the neuron when at rest maintains an electric potential difference
Define the Micronutrient Deficiencies? In the previous section, we learnt about macronutrient deficiencies i.e. protein energy malnutrition in children. We are now aware that t
Explain Proteins as lubricants? The mucous of the respiratory tract, oral cavity, vaginal tract and the rectal cavity reduces the irritation which might be caused by materials
CEL L BODY (= CYTON OR SOMA) OR PERIKARYON - It is nucleated & broader part of neuron. It may be up to 13.5 mm in diameter and may be irregular, spherical, oval, rounded
Q. Cnidarian identity card. How are they characterized according to instance of representing beings, basic morphology, kind of symmetry, germ layers and coelom, digestive system, r
how does the hypothalamus begin puberty
International organization and zoonoses In 1962, the Food and Agriculture Organization and the World Health Organization created the Codex Alimentarius Commission (Codex) to e
Explain the NATURAL HISTORY OF CORONARY ARTERY DISEASES (GAD)? The natural history of CAD is very important from the preventive point or view. Though the usual manifestations o
Q. Explain about Catheters and Guide Wires? The commonly used guide wires vary in diameter from 0.012 to 0.052; with 0.035 or 0.038 being the most commonly used sizes. The stan
Q. Classify the diversity of organism in different groups • The goal of taxonomy is to develop a workable classification that reflects evolutionary relationships and provides
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd