Antibodies, Biology

Assignment Help:

Antibodies

Antibodies are important.tools for detecting and localising specific molecules in the cells due to their high specificity. The first requirement for this is to produce antibodies against a specific molecule. Suppose you want to produce antibodies against tubulin, the purified tubulin is first obtained from a given tissue. It is then injected into an animal such as rabbit which in nwse to tubulin produces antibodies. Blood sample is collected from the animal and antibodies are isolated. These antibodies are used to locakise the specific molecule in the cell because they bind only 80 an antigen, which is a tubulia in this case. Localisation can be done by two methods: direct and inditect

In the direct method, antibodies are first labelled with a flouroscendt dye-like rhodamide for light microscopy, or witha high molecular weight, electron dense compound like ferritin for transmission electron microscopy. Fluorescent labelled antibodies are allowed to react with cells so as to form systematic antibody complex. The complex is then examined under
a microscope (fluorescent microscope or TEM). The specific molecules will emit light or become electron dense due to their binding with labelled antibodies (Figure shown below). This method is used to locdise foreign bodies such us viruses, bacterial antigevs, etc.

618_antibodies.jpg


Related Discussions:- Antibodies

Concerning the number of hosts how are parasites classified, Q. Concerning ...

Q. Concerning the number of hosts how are parasites classified? The Parasites that require only one host are called as monoxenous parasites. The Parasites that need more than o

Chances of change in the gene pool, A genetic trait which is considered to ...

A genetic trait which is considered to be harmful in the present day might prove to be beneficial in future. For example by removing the trait for sickle cell we may increase the s

Explain about riboflavin, Explain about Riboflavin Aqueous solution sh...

Explain about Riboflavin Aqueous solution shows a pronounced green-yellow fluorescence, which is maximal at a pH of about, 6-7 and disappears upon the addition of acids and al

Describe the shape and size of proteins, Shape and size of protiens ...

Shape and size of protiens Fibrous proteins for example: keratin in hair, actin and myosin in muscles, and collagen. Globular proteins, for example enzymes and antibo

Kingdom animalia, in what order did the shellfish belong? in what genus did...

in what order did the shellfish belong? in what genus did the shellfish belong? in what species did the shellfish belong?

The basics of dna cloning, There  are a broad  range  of various  process  ...

There  are a broad  range  of various  process  for cloning  DNA  into  either viral  or plasmid vectors  but the basic  scheme  of events  is frequently  same.  To clone into a pl

Determine effective half life and value of effective decay, 1. By direct me...

1. By direct measurement of the activity of the thyroid gland in several patients who had received 131 I for diagnostic purposes, it was observed that the biological elimination h

What are secondary roots, What are secondary roots? In origin how secondary...

What are secondary roots? In origin how secondary roots differentiate from shoots? Secondary roots are ramifications of the primary (major) root. The secondary roots emerge fro

Determine nutritional requirements for space missions, Determine Nutritiona...

Determine Nutritional Requirements for Space Missions? Space exploration represents a new frontier in the nutritional sciences and humans are eating in space since Cosmonaut Yu

How simple transposition of the great arteries present, How Simple Transpos...

How Simple Transposition of the Great Arteries Present after 30 Days ? A switch operation will have poor results, as the ventricle that has regressed will not be able to mainta

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd