Antibodies, Biology

Assignment Help:

Antibodies

Antibodies are important.tools for detecting and localising specific molecules in the cells due to their high specificity. The first requirement for this is to produce antibodies against a specific molecule. Suppose you want to produce antibodies against tubulin, the purified tubulin is first obtained from a given tissue. It is then injected into an animal such as rabbit which in nwse to tubulin produces antibodies. Blood sample is collected from the animal and antibodies are isolated. These antibodies are used to locakise the specific molecule in the cell because they bind only 80 an antigen, which is a tubulia in this case. Localisation can be done by two methods: direct and inditect

In the direct method, antibodies are first labelled with a flouroscendt dye-like rhodamide for light microscopy, or witha high molecular weight, electron dense compound like ferritin for transmission electron microscopy. Fluorescent labelled antibodies are allowed to react with cells so as to form systematic antibody complex. The complex is then examined under
a microscope (fluorescent microscope or TEM). The specific molecules will emit light or become electron dense due to their binding with labelled antibodies (Figure shown below). This method is used to locdise foreign bodies such us viruses, bacterial antigevs, etc.

618_antibodies.jpg


Related Discussions:- Antibodies

Determine cdna encodes aba 8-hydroxylase activity, Hypothetical question. ...

Hypothetical question. A new biotech company intends to clone the gene encoding the ABA 8-hydroylase enzyme, an important protein regulating plant hormone degradation that control

Can you explain tannins, Q. Can you explain Tannins? Tannins are anoth...

Q. Can you explain Tannins? Tannins are another class of compounds which interfere with the absorption of minerals like iron and reduce the availability of proteins by bindin

Acid-base imbalances from appearing in the body, List and briefly describe ...

List and briefly describe the mechanisms that prevent acid-base imbalances from appearing in the body. In addition, indicate where each mechanism functions most significantly.

The final concentration of the solution, Assume that you have 1 ml of a sol...

Assume that you have 1 ml of a solution of amylase (an enzyme) at a concentration of 15 mg protein/ml. Calculate the volume of diluting buffer that you would have to add to 1.0 ml

Biotechnology, How does bisulfite sequencing work ?

How does bisulfite sequencing work ?

Explain about the optic disc, Explain about the optic disc The optic di...

Explain about the optic disc The optic disc is also called a blind spot while the macula is referred to as the yellow spot. The ora serrata (the intersection between the retina

Advantage of using a patient''s own stem cells, What would be the advantage...

What would be the advantage of using a patient's own stem cells e.g. blood stem cells, to treat his or her illness? The benefit of using stem cells from the patient being trea

What would the priorities be for allocating, Agriculture, industry, and gro...

Agriculture, industry, and growing populations of cities all compete, through political influence, for water. If you were in charge of water resources in an arid region, what would

Determine the types of cell, Determine the types of Cell Based OII the ...

Determine the types of Cell Based OII the degree of complexity of the cell organisation, two types of cells, namely, prokaryotes and eukaryotes, are recognised. The prokaryotic

Blood pressure - blood vessels, Normal 0 false false false ...

Normal 0 false false false EN-IN X-NONE X-NONE MicrosoftInternetExplorer4

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd