Antibodies, Biology

Assignment Help:

Antibodies

Antibodies are important.tools for detecting and localising specific molecules in the cells due to their high specificity. The first requirement for this is to produce antibodies against a specific molecule. Suppose you want to produce antibodies against tubulin, the purified tubulin is first obtained from a given tissue. It is then injected into an animal such as rabbit which in nwse to tubulin produces antibodies. Blood sample is collected from the animal and antibodies are isolated. These antibodies are used to locakise the specific molecule in the cell because they bind only 80 an antigen, which is a tubulia in this case. Localisation can be done by two methods: direct and inditect

In the direct method, antibodies are first labelled with a flouroscendt dye-like rhodamide for light microscopy, or witha high molecular weight, electron dense compound like ferritin for transmission electron microscopy. Fluorescent labelled antibodies are allowed to react with cells so as to form systematic antibody complex. The complex is then examined under
a microscope (fluorescent microscope or TEM). The specific molecules will emit light or become electron dense due to their binding with labelled antibodies (Figure shown below). This method is used to locdise foreign bodies such us viruses, bacterial antigevs, etc.

618_antibodies.jpg


Related Discussions:- Antibodies

Sexual reproduction, SEXUA L REPRODUCTION 1 .       Amphigony       ...

SEXUA L REPRODUCTION 1 .       Amphigony        -          Zygote is formed. (i) Syngamy Fusion of gametes. (a) Endogamy - It involves self fertilisation. e.g.

Assignment, Write short note onApplications of apical dominance in horticul...

Write short note onApplications of apical dominance in horticulture and agriculture

Histogenesis and organogenesis, Histogenesis and Organogenesis After t...

Histogenesis and Organogenesis After the octant stage numerous cell divisions occur in various planes. The proembryo become globular or bulb-shaped. As a general rule, some ta

Can you explain mitotic apparatus, Q. What is mitotic apparatus? Mitoti...

Q. What is mitotic apparatus? Mitotic apparatus is the set of aster fibers, radial structures around each centriole pair, plus the spindle fibers, fibers that extend across the

Azotobacter, Azotobacter Azotobacter is known to possess the following...

Azotobacter Azotobacter is known to possess the following two mechanisms to protect its nitrogenase from oxygen inhibition. Respiratory protection and Confor

Protozoa, Characteristics Protozoa and examples

Characteristics Protozoa and examples

Osmosis, If you have water, 10% sucrose, and 40% sucrose solution. Which so...

If you have water, 10% sucrose, and 40% sucrose solution. Which solutions were isotonic to each other?

Peste des petits ruminants (ppr), Peste des petits ruminants (PPR) is an a...

Peste des petits ruminants (PPR) is an acute, highly contagious viral disease of goats and sheep caused by peste des petits ruminants (PPR) virus which belongs to the genus Morbil

Post disease as a result -endodontists, If the condition or the post diseas...

If the condition or the post disease as a result of intraradicular infection----> treated conventionally via apex " non-surgical ", ---->Here we have 2 options either coronal

What are the functions of the spleen, What are the functions of the spleen?...

What are the functions of the spleen? Why is a total splenectomy (surgical removal of the spleen) compatible with life? The spleen has many functions: it participates in the de

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd