Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Antibodies
Antibodies are important.tools for detecting and localising specific molecules in the cells due to their high specificity. The first requirement for this is to produce antibodies against a specific molecule. Suppose you want to produce antibodies against tubulin, the purified tubulin is first obtained from a given tissue. It is then injected into an animal such as rabbit which in nwse to tubulin produces antibodies. Blood sample is collected from the animal and antibodies are isolated. These antibodies are used to locakise the specific molecule in the cell because they bind only 80 an antigen, which is a tubulia in this case. Localisation can be done by two methods: direct and inditect In the direct method, antibodies are first labelled with a flouroscendt dye-like rhodamide for light microscopy, or witha high molecular weight, electron dense compound like ferritin for transmission electron microscopy. Fluorescent labelled antibodies are allowed to react with cells so as to form systematic antibody complex. The complex is then examined undera microscope (fluorescent microscope or TEM). The specific molecules will emit light or become electron dense due to their binding with labelled antibodies (Figure shown below). This method is used to locdise foreign bodies such us viruses, bacterial antigevs, etc.
State the Designations for Horizons Of the several horizons, the master horizons are the results of the fundamental soil forming processes, viz. humification, eluviation and
Note on production of haploid by tissue culture.
What is the normal duration of the menstrual cycle? How does the calendar contraceptive method work? The normal duration of the menstrual cycle is 28 days but it can vary betwe
Q. Show the Use of Incineration for sterilization? This is an excellent method for rapidly destroying materials such as soiled dressings, animal carcasses, bedding and patholog
Q. What is the usual morphological feature of nematodes that differentiates them from platyhelminthes? Nematodes are also called as roundworms. As the name indicates they are n
Q. What do you understand by global warming? The Global warming is the increase in the temperature of the planet due to accumulation of some gases in the atmosphere, especially
Algal or Planktonic Fish Poisonings: Fish poisoning can result from the ingestion of fish or shellfish that have fed upon algae toxic to human beings. Paralytic shellfish poisoning
As a result of DNA replication two DNA molecules come into existence. Why is it not correct to assert that two "new" DNA molecules are created? What is the name given to the proces
Besides the XY system are there other sex determination systems? A few animals have a sex determination system different from the XY system such as the X0 system is the sex det
A charged particle A exerts a force of 2.39uN to the right on charged particle B when the particles are 12.3 mm apart. Particle B moves straight away from A to make the distance be
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd