Antibodies, Biology

Assignment Help:

Antibodies

Antibodies are important.tools for detecting and localising specific molecules in the cells due to their high specificity. The first requirement for this is to produce antibodies against a specific molecule. Suppose you want to produce antibodies against tubulin, the purified tubulin is first obtained from a given tissue. It is then injected into an animal such as rabbit which in nwse to tubulin produces antibodies. Blood sample is collected from the animal and antibodies are isolated. These antibodies are used to locakise the specific molecule in the cell because they bind only 80 an antigen, which is a tubulia in this case. Localisation can be done by two methods: direct and inditect

In the direct method, antibodies are first labelled with a flouroscendt dye-like rhodamide for light microscopy, or witha high molecular weight, electron dense compound like ferritin for transmission electron microscopy. Fluorescent labelled antibodies are allowed to react with cells so as to form systematic antibody complex. The complex is then examined under
a microscope (fluorescent microscope or TEM). The specific molecules will emit light or become electron dense due to their binding with labelled antibodies (Figure shown below). This method is used to locdise foreign bodies such us viruses, bacterial antigevs, etc.

618_antibodies.jpg


Related Discussions:- Antibodies

Nature defences against viral infections, Nature defences against viral inf...

Nature defences against viral infections 1.      Interferons - In 1957 Issue and Lindenmann discovered that vertebrate cells infected with viruses, produce a diffusible antiv

Disease prevention for the high risk group, Disease Prevention for the High...

Disease Prevention for the High Risk Group   Interventions aimed at early diagnosis and appropriate management for reducing morbidity and mortality targeting people who suffer

Demonstrate the counselling process in the form of role play, Q. Demonstrat...

Q. Demonstrate the counselling process in the form of role play? Role playing refers either to the unconscious changing of one's behavior to assume a social role or roles in lif

Explain the term- pedon and polypedon, Explain the term- pedon and polypedo...

Explain the term- pedon and polypedon The characteristics of soil do not change vertically downwards only, there are lateral variations also as soil is a three dimensional body.

What do you mean by osteichthyes and condrichthyes, Q. From which features ...

Q. From which features do osteichthyes and condrichthyes get these names? "Chondros" means cartilage, "ictis" means fish (both from the Greek) the name chondrichtians is for fi

State the classification of nervous system, State the Classification of ner...

State the Classification of nervous system Neurological disorders can be categorised according to the primary location affected, the primary type of dysfunction involved, or th

Female reproductive system - fallopian tube, F ALLOPIAN TUBE - Ferti...

F ALLOPIAN TUBE - Fertilisation occur in it. 10-12 cm long muscular tube. It is supported by double fold of peritonium. It shows 4 regions - (i ) Infundibulum - Bro

Define role of public nutritionist in health care delivery, Define the Role...

Define the Role of Public Nutritionist in Health Care Delivery? It is clearly evident from the foregoing discussion that nutrition is an important, though not the only, determi

List the areas of educating a diabetic patient, Q. List the areas of educat...

Q. List the areas of educating a diabetic patient? 1 Self monitoring of Blood -glucose 2 Urine testing 3 Hypoglycaemia 4 Self Administration of Insulin 5 Foot Care

Major renal processes that combined produce urine, Q. What are the three ma...

Q. What are the three major renal processes that combined produce urine? Urine is made by the occurrence of three processes in the nephron that are tubular resorption, glomerul

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd