Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Antibodies
Antibodies are important.tools for detecting and localising specific molecules in the cells due to their high specificity. The first requirement for this is to produce antibodies against a specific molecule. Suppose you want to produce antibodies against tubulin, the purified tubulin is first obtained from a given tissue. It is then injected into an animal such as rabbit which in nwse to tubulin produces antibodies. Blood sample is collected from the animal and antibodies are isolated. These antibodies are used to locakise the specific molecule in the cell because they bind only 80 an antigen, which is a tubulia in this case. Localisation can be done by two methods: direct and inditect In the direct method, antibodies are first labelled with a flouroscendt dye-like rhodamide for light microscopy, or witha high molecular weight, electron dense compound like ferritin for transmission electron microscopy. Fluorescent labelled antibodies are allowed to react with cells so as to form systematic antibody complex. The complex is then examined undera microscope (fluorescent microscope or TEM). The specific molecules will emit light or become electron dense due to their binding with labelled antibodies (Figure shown below). This method is used to locdise foreign bodies such us viruses, bacterial antigevs, etc.
Q. How does the plant cell wall act in response when it is placed under hypotonic medium? The plant cell wall (the covering of the cell external to the cell membrane) is made o
what websites can i use to modify my client''s meals?
Explain the organisation of the intensive care unit? The following kinds of professional groups are required in the day to day functioning of a modern intensive care unit: Th
What is the difference between an acid and a base? Base is anything, which has a capability to abstract a proton. Using the easiest definition, an acid is something when add
Placement of implant with improper axial angulation Surgical placement of the implant improperly can lead to a prosthetic rehabilitation that compromises esthetics and the dist
Butane and 2-methyl-propane are: Select one: a. Alcohols b. geometric isomers c. structural isomers d. Stereoisomers e. All of the above
How to Defibrillate? Three shocks should be given initially in a 200J - 200J - 360J sequence. For most recoverable situations, 200J would be adequate and results in least myco
Organisational Behaviour of Health Care The insights provided by the theories of organisational behaviour emphasise the need for the establishment of institutional frameworks
Q. Which type of virus is the HIV? What is the enzyme reverse transcriptase present in HIV? HIV is a retrovirus that is RNA viral (its genetic material is RNA and not DNA).
what are the unit for restriction enzyme activity?
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd