Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Antibodies
Antibodies are important.tools for detecting and localising specific molecules in the cells due to their high specificity. The first requirement for this is to produce antibodies against a specific molecule. Suppose you want to produce antibodies against tubulin, the purified tubulin is first obtained from a given tissue. It is then injected into an animal such as rabbit which in nwse to tubulin produces antibodies. Blood sample is collected from the animal and antibodies are isolated. These antibodies are used to locakise the specific molecule in the cell because they bind only 80 an antigen, which is a tubulia in this case. Localisation can be done by two methods: direct and inditect In the direct method, antibodies are first labelled with a flouroscendt dye-like rhodamide for light microscopy, or witha high molecular weight, electron dense compound like ferritin for transmission electron microscopy. Fluorescent labelled antibodies are allowed to react with cells so as to form systematic antibody complex. The complex is then examined undera microscope (fluorescent microscope or TEM). The specific molecules will emit light or become electron dense due to their binding with labelled antibodies (Figure shown below). This method is used to locdise foreign bodies such us viruses, bacterial antigevs, etc.
Define Iodothyronine Deiodinases - functions of selenium in humans? Another group of selenoproteins are the iodothyronine deiodinases essential for the conversion of thyroxine
Determine the Food Sources of Pyridoxine? Raw foods contain more of this vitamin than cooked foods. The food sources include: 1. Rich sources: Rice polishing s, wheat bran,
Heterochromatin: Compact, gene-poor areas of a genome, which are enriched in the simple sequence repeats. As it may be impossible to clone, heterochromatin is generally ignored wh
What are the plant organs responsible for the perception of light variation? What is the pigment responsible for this perception? Leaves are mostly responsible for perception o
describe the endoskeleton of fish?
biology ofr this
In biological studies which of the below terms is used to refer to muscles with less than normal tone? Is it: a) Latent b) Atonic c) Flaccid d) None of these ANSWE
BLOO D GROUPS- In R.B.C. on plasmalemma antigen or agglutinogen present form antibodies or agglutinines. Blood groups are based on antigens. 1 . ABO SYSTEM -
What is the indolacetic acid (IAA)? IAA or Indolacetic acid (indolyl-3-acetic acid) is the major natural auxin made by plants. It promotes plant growth and cellular differentia
What are the cell types that form the phloem? What are the main features of those cells? The major cells that form the phloem are the sieve elements and the companion cells. Th
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd