Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Antibodies
Antibodies are important.tools for detecting and localising specific molecules in the cells due to their high specificity. The first requirement for this is to produce antibodies against a specific molecule. Suppose you want to produce antibodies against tubulin, the purified tubulin is first obtained from a given tissue. It is then injected into an animal such as rabbit which in nwse to tubulin produces antibodies. Blood sample is collected from the animal and antibodies are isolated. These antibodies are used to locakise the specific molecule in the cell because they bind only 80 an antigen, which is a tubulia in this case. Localisation can be done by two methods: direct and inditect In the direct method, antibodies are first labelled with a flouroscendt dye-like rhodamide for light microscopy, or witha high molecular weight, electron dense compound like ferritin for transmission electron microscopy. Fluorescent labelled antibodies are allowed to react with cells so as to form systematic antibody complex. The complex is then examined undera microscope (fluorescent microscope or TEM). The specific molecules will emit light or become electron dense due to their binding with labelled antibodies (Figure shown below). This method is used to locdise foreign bodies such us viruses, bacterial antigevs, etc.
What is speciation? The Speciation is the process by which different species emerge from a common ancestor species. The Speciation generally begins when populations of the same
Reproductive Cycle The whole reproductive cycle consists of an ovarian cycle and a uterine cycle. Figure illustrates one complete reproductive cycle. You can observe in the fi
B o v i ne viral diarrhoea Bovine viral diarrhoea (BVD) and mucosal disease (MD) are clinically dissimilar disease syndrome yet have a common viral etiology. The acute dise
locomotion in parameceum
(i) Effect of Human Health: (a) Mercury compounds in waste water in converted into methyl mercury by Bacterial action which causes numbness of limbs, lips and tongue, deaf
Reed-Swamp Stage - Hydrarch This stage is also known as amphibious stage, as the plants of the community are rooted but most parts of their shoots remain exposed to air. Speci
Countercurrent flow is the arrangement by which fish get oxygen from the water which flows by the help of their gills. The water flows across the respiratory surface of the gill i
Explain about the Niacin - Water Soluble Vitamin? Niacin is chemically synonymous with nicotinic acid although the term is also used for its amide (nicotinamide). Nicotinamide
Brooke's Formula: a) Fluid requirement b) Estimate the accurate/approximate weight of the patient c) First 24 hours Colloids (blood, plasma, dextran) 0.5 ml
Write in 3-4 lines about foot examination Foot ulceration and amputation are common and major causes of morbidity and disability in people with diabetes. Skin integrity should
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd