Antibodies, Biology

Assignment Help:

Antibodies

Antibodies are important.tools for detecting and localising specific molecules in the cells due to their high specificity. The first requirement for this is to produce antibodies against a specific molecule. Suppose you want to produce antibodies against tubulin, the purified tubulin is first obtained from a given tissue. It is then injected into an animal such as rabbit which in nwse to tubulin produces antibodies. Blood sample is collected from the animal and antibodies are isolated. These antibodies are used to locakise the specific molecule in the cell because they bind only 80 an antigen, which is a tubulia in this case. Localisation can be done by two methods: direct and inditect

In the direct method, antibodies are first labelled with a flouroscendt dye-like rhodamide for light microscopy, or witha high molecular weight, electron dense compound like ferritin for transmission electron microscopy. Fluorescent labelled antibodies are allowed to react with cells so as to form systematic antibody complex. The complex is then examined under
a microscope (fluorescent microscope or TEM). The specific molecules will emit light or become electron dense due to their binding with labelled antibodies (Figure shown below). This method is used to locdise foreign bodies such us viruses, bacterial antigevs, etc.

618_antibodies.jpg


Related Discussions:- Antibodies

Factors affecting normal blood sugar levels, Q. Factors Affecting Normal Bl...

Q. Factors Affecting Normal Blood Sugar Levels? Earlier in our discussion, we learnt that insulin is the most important factor responsible for normal blood sugar levels. Now we

What are the most important worm infections, Q. What are the most important...

Q. What are the most important worm infections? The most significant human worm infections are taeniasis, schistosomiasis, cysticercosis, ascariasis and ancylostomiasis (hookwo

Explain the physical methods to control microorganisms, Explain the Physica...

Explain the Physical Methods to Control Microorganisms? The physical methods to control microorganisms involve heat, filtration or radiations. Figure illustrates these methods.

Define effect of fat on quality and quantity of human milk, Define effect o...

Define effect of Fat on quality and quantity of human milk? Fat content of milk appears to be subject to variability as compared to other constituents. The average fat content

Explain demography and demographic transition, Explain Demography, Demograp...

Explain Demography, Demographic Transition and Demographic Cycle We know that population of different countries in the world is always changing. For some countries like India a

Clinical manifestations of bronchial asthma, Clinical Manifestations C...

Clinical Manifestations Can be chronic to acute, mild to severe. Acute attack often occur at night. During an acute attack, audible inspiratory and expiratory wheezing. Patie

How do taenias classify according to the division of sexes, How do taenias ...

How do taenias classify according to the division of sexes? Taenias are monoecious (hermaphrodite), the similar individual has female and male reproductive organs and undergoes

Define mechanisms for antinutritional effect on human, Define mechanisms fo...

Define mechanisms for antinutritional effect on Human? There are several possibilities: reduced availability of amino acids to cancer cells by reversing the carcinogen-induced

An a=t mispairing leads to an a=c substitution, An A=T mispairing leads to ...

An A=T mispairing leads to an A=C substitution. The other DNA helix will contain a(n) __ pair. a. A=C b. A=T c. G=C d. B=Q e. T=T Can you also explain it please so if I'm ask

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd