Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Antibodies
Antibodies are important.tools for detecting and localising specific molecules in the cells due to their high specificity. The first requirement for this is to produce antibodies against a specific molecule. Suppose you want to produce antibodies against tubulin, the purified tubulin is first obtained from a given tissue. It is then injected into an animal such as rabbit which in nwse to tubulin produces antibodies. Blood sample is collected from the animal and antibodies are isolated. These antibodies are used to locakise the specific molecule in the cell because they bind only 80 an antigen, which is a tubulia in this case. Localisation can be done by two methods: direct and inditect In the direct method, antibodies are first labelled with a flouroscendt dye-like rhodamide for light microscopy, or witha high molecular weight, electron dense compound like ferritin for transmission electron microscopy. Fluorescent labelled antibodies are allowed to react with cells so as to form systematic antibody complex. The complex is then examined undera microscope (fluorescent microscope or TEM). The specific molecules will emit light or become electron dense due to their binding with labelled antibodies (Figure shown below). This method is used to locdise foreign bodies such us viruses, bacterial antigevs, etc.
TRISACCHARIDES The oligosaccharides are made of three monosaccharide residues. A common trisaccharides is Raffinose which is formed by condensation of galactose-glucose-fr
Q. What are compound eyes? Arthropods have compound eyes made of several visual units called as ommatidia. Each ommatidium transmits visual information through the optic nerve
Five Kingdom Classifications Biology, as you know, is the study of life, living things, and their relationship to one another and to their environment. This branch of Science
1. Given a log phase bacterial culture with 1 x 106 cells per ml and a generation time of 20 minutes, how long does it take the culture to reach a density of 6.4 ? 107 cells per ml
Gametogenesis - Human Development Gametogenesis as you are responsive is the process of formation and development of specialized reproductive cells, ova in females and sperms
Q. What is the contraceptive mechanism of the IUD? The IUD (intrauterine device) is a piece of plastic coated with copper that is put-in within the uterus by a doctor. Coppe
Parthenogenesis The diploid egg produced in the embryo sacs during diplospory and apospory develops into an embryo without fertilization, thus maintaining the sporophytic lev
Biochemical Changes Associated with Senescence When senescence begins many physiological and biochemical changes take place. For example, one of the important changes observed
Colibacillosis of poultry Colibacillosis is an important disease among poultry, especially among broiler chicks aged 6-9 weeks and less frequently of young chicks during first
How different is the growth according to the biotic potential of a viral population from the growth according to the biotic potential of a bacterial population? The growth cur
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd