Antibodies, Biology

Assignment Help:

Antibodies

Antibodies are important.tools for detecting and localising specific molecules in the cells due to their high specificity. The first requirement for this is to produce antibodies against a specific molecule. Suppose you want to produce antibodies against tubulin, the purified tubulin is first obtained from a given tissue. It is then injected into an animal such as rabbit which in nwse to tubulin produces antibodies. Blood sample is collected from the animal and antibodies are isolated. These antibodies are used to locakise the specific molecule in the cell because they bind only 80 an antigen, which is a tubulia in this case. Localisation can be done by two methods: direct and inditect

In the direct method, antibodies are first labelled with a flouroscendt dye-like rhodamide for light microscopy, or witha high molecular weight, electron dense compound like ferritin for transmission electron microscopy. Fluorescent labelled antibodies are allowed to react with cells so as to form systematic antibody complex. The complex is then examined under
a microscope (fluorescent microscope or TEM). The specific molecules will emit light or become electron dense due to their binding with labelled antibodies (Figure shown below). This method is used to locdise foreign bodies such us viruses, bacterial antigevs, etc.

618_antibodies.jpg


Related Discussions:- Antibodies

What is cold-shortening, What is cold-shortening It is important to poi...

What is cold-shortening It is important to point out that over-effective chilling of hot carcasses can lead to toughness. If the temperature of the meat (muscles) can be reduce

Explain transfer from solid culture to liquid media, Explain Transfer from ...

Explain Transfer from Solid Culture to Liquid Media The steps involved in this technique are included herewith: 1. Sterilized inoculating loop or needle is touched carefully

Why d scientists pick hydrogen as the basis for mri scanning, Why d scienti...

Why d scientists pick hydrogen as the basis for MRI scanning? Name of the human body that do not appear in an MRI scan. Explain thermal stratification? How does thermal stratifi

Direction of flow, Direction of flow--either towards or away from the trans...

Direction of flow--either towards or away from the transducer (positive or negative Doppler shifts). Timing-instantaneous velocity and direction of flow throughout the various

Nutrition, The oxidation of sugar in the cell of higher organisms takes pla...

The oxidation of sugar in the cell of higher organisms takes place in the where

Nutrient requirements for preterm and low birth weight, Define the Nutrient...

Define the Nutrient requirements for preterm and low birth weight? Energy: For preterm infants 1.20 Kcal/kg/day,            For normal infants 108 Kcal/ kg/ day. Proteins

Explain food applications of xanthan, Explain Food Applications of xanthan ...

Explain Food Applications of xanthan Xanthan gum is mainly considered to be non-gelling and used for the control of viscosity due to the tenuous associations, endowing it w

Animal classification, Explain metachrosis in frogs? How it is different fr...

Explain metachrosis in frogs? How it is different from change of skin colour of chemleon?

What do you understand by metanephridia, What do you understand by Metaneph...

What do you understand by Metanephridia? An excretory-osmoregulatory organ comprising a ciliated funnel, nephrostome, connected to tubules that lead to the external nephridiopo

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd