Antibodies, Biology

Assignment Help:

Antibodies

Antibodies are important.tools for detecting and localising specific molecules in the cells due to their high specificity. The first requirement for this is to produce antibodies against a specific molecule. Suppose you want to produce antibodies against tubulin, the purified tubulin is first obtained from a given tissue. It is then injected into an animal such as rabbit which in nwse to tubulin produces antibodies. Blood sample is collected from the animal and antibodies are isolated. These antibodies are used to locakise the specific molecule in the cell because they bind only 80 an antigen, which is a tubulia in this case. Localisation can be done by two methods: direct and inditect

In the direct method, antibodies are first labelled with a flouroscendt dye-like rhodamide for light microscopy, or witha high molecular weight, electron dense compound like ferritin for transmission electron microscopy. Fluorescent labelled antibodies are allowed to react with cells so as to form systematic antibody complex. The complex is then examined under
a microscope (fluorescent microscope or TEM). The specific molecules will emit light or become electron dense due to their binding with labelled antibodies (Figure shown below). This method is used to locdise foreign bodies such us viruses, bacterial antigevs, etc.

618_antibodies.jpg


Related Discussions:- Antibodies

Drawbacks of lamellar model, Drawbacks of Lamellar model This model ...

Drawbacks of Lamellar model This model does not explain the elasticity of PM. Although this model says that PM is elastic but it also states that head of lipid molecules is

What is deplasmolysis of plant cells, What is deplasmolysis of plant cells?...

What is deplasmolysis of plant cells? The plant cell when placed under hypertonic medium loses a huge amount of water and its cell membrane detaches from the cell wall. In that

Pathophysiology and assessment of iron deficiency anaemia, Pathophysiology ...

Pathophysiology   Insufficient  amount of iron present in the body leads  to reduction in serum transferrin (Serum betaglobulin, which binds and transports iron) saturation.  T

Before purchasing - nursing software, Before Purchasing  Ideally it...

Before Purchasing  Ideally it's wise to buy a readymade software with options to further customize it to suit your managerial functions.   A totally Custom-made softwar

Use of alternate metabolic pathways, Use of Alternate Metabolic Pathways ...

Use of Alternate Metabolic Pathways Plants surviving under water-logged conditions offer the simplest example of such a response. The submerged parts of such plants use the an

What is the route of nutrients after digestion, Q. From the intestinal lume...

Q. From the intestinal lumen through to the tissues - what is the route of nutrients after digestion? Monosaccharides, mineral salts, amino acids and water are absorbed by the

What do biomass pyramids represent, What do biomass pyramids represent? ...

What do biomass pyramids represent? Biomass pyramids show the sum of the masses of the individuals that participate in each trophic level of a food chain.

Animals of the open water zone, Animals of the open water zone The li...

Animals of the open water zone The limnetic region of this zone contains certain fishes as well as rotifers, zooplankton such as crustacean and protozoan. In the profundal zo

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd