Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Antibodies
Antibodies are important.tools for detecting and localising specific molecules in the cells due to their high specificity. The first requirement for this is to produce antibodies against a specific molecule. Suppose you want to produce antibodies against tubulin, the purified tubulin is first obtained from a given tissue. It is then injected into an animal such as rabbit which in nwse to tubulin produces antibodies. Blood sample is collected from the animal and antibodies are isolated. These antibodies are used to locakise the specific molecule in the cell because they bind only 80 an antigen, which is a tubulia in this case. Localisation can be done by two methods: direct and inditect In the direct method, antibodies are first labelled with a flouroscendt dye-like rhodamide for light microscopy, or witha high molecular weight, electron dense compound like ferritin for transmission electron microscopy. Fluorescent labelled antibodies are allowed to react with cells so as to form systematic antibody complex. The complex is then examined undera microscope (fluorescent microscope or TEM). The specific molecules will emit light or become electron dense due to their binding with labelled antibodies (Figure shown below). This method is used to locdise foreign bodies such us viruses, bacterial antigevs, etc.
Q. Does the fish heart pump venous or arterial blood? After oxygenation in the gills the arterial blood goes to the tissues so the fish heart pumps venous blood, the venous blo
Coronary Arteries The right coronary artery originates form the right aortic valvular cusp, its, SA nodal branch supplies the SA node and AV nodal branch supply the AV nod
Choose the correct answer. A. The phenotype determines the genotype. B. True-breeding individuals are produced by repeated backcrossing. C. Recessive alleles are usually loss-of-fu
Calculate the pH - microbial survival and growth? Presence of microorganisms also depends on the pH of the medium. Most bacteria grow optimally near neutral pH (optimum 6.5 - 7
Q. What is Regional wall motion abnormality? Ans. A critical occlusion of a coronary artery leads to myocardial ischemia which is manifested as regional wall motion abnorm
HEAR T - The cells called cardiocytes of atria of the heart secrete peptide hormone called atrial natriuretic factor (ANF) in response of an increased return of the deoxygen
right classification of protozoa up to class
Q. Show Foods that damage GI mucosa? Foods that damage GI mucosa: A number of spices, herbs and other condiments have been found to have little or no irritating effect on the m
Circuitry and Priming : The cardio pulmonary bypass circuit consists of oxygenator, tubings, cannulae, cardiotomy reservoirs and cardioplegia attachments. The first step is to pri
Feeding mechanism in holozoic animal
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd