Antibodies, Biology

Assignment Help:

Antibodies

Antibodies are important.tools for detecting and localising specific molecules in the cells due to their high specificity. The first requirement for this is to produce antibodies against a specific molecule. Suppose you want to produce antibodies against tubulin, the purified tubulin is first obtained from a given tissue. It is then injected into an animal such as rabbit which in nwse to tubulin produces antibodies. Blood sample is collected from the animal and antibodies are isolated. These antibodies are used to locakise the specific molecule in the cell because they bind only 80 an antigen, which is a tubulia in this case. Localisation can be done by two methods: direct and inditect

In the direct method, antibodies are first labelled with a flouroscendt dye-like rhodamide for light microscopy, or witha high molecular weight, electron dense compound like ferritin for transmission electron microscopy. Fluorescent labelled antibodies are allowed to react with cells so as to form systematic antibody complex. The complex is then examined under
a microscope (fluorescent microscope or TEM). The specific molecules will emit light or become electron dense due to their binding with labelled antibodies (Figure shown below). This method is used to locdise foreign bodies such us viruses, bacterial antigevs, etc.

618_antibodies.jpg


Related Discussions:- Antibodies

Metabolism, Metabolism Metabolism as you know is a process by which li...

Metabolism Metabolism as you know is a process by which living organisms acquire and use energy. A large number of biochemical reactions take place in the living organisms dur

General process of post-hatching growth in insects, General process of post...

General process of post-hatching growth in insects While a young insect hatches out from an egg it is covered by a firm, inflexible, sclerotized cuticle, which because of its

Explain adverse effects of abacavir, Adverse effects of Abacavir  In 3-...

Adverse effects of Abacavir  In 3-9% of patients, a severe hypersensitivity reaction, usually with fever and sometimes with respiratory or gastrointestinal symptoms, malaise an

Classifications of carbohydrates based on number of carbon, Define the Clas...

Define the Classifications of carbohydrates based on number of carbon?   A chain-form monosaccharide that has a carbonyl group (C= O) on an end carbon forming an aldehy

Microbiology, Bacteria, mutations, recombination, evolution, and adaption, ...

Bacteria, mutations, recombination, evolution, and adaption, how are all these related; must be written in two sentences.

Show photosynthesis process in algae and plants, Q. Which all are the livin...

Q. Which all are the living beings that carry out photosynthesis? Which is the cell organelle responsible for the absorption of light for the photosynthesis process in algae and pl

Explain reproduction rates variation in bacterial gene, Antibiotic resistan...

Antibiotic resistance is the result of natural selection mutations, the only source of new genetic information available for bacteria. Rapid reproduction rates variation in bacteri

Malthus theory of human population growth, MA L THU S THEORY OF HUMAN PO...

MA L THU S THEORY OF HUMAN POPULATION GROWTH - Malthus put forward in 1778. 1.       Population grows in geometrical ratio, where as the means grow in arithmetical ratio.

Processing grains and oil seeds for animal feeding, Processing grains and o...

Processing grains and oil seeds for animal feeding Simple processing of grains such as grinding facilitates the digestion particularly in cattle and buffaloes. Fine grinding of

List the four phases of mitosis, List the four phases of mitosis, and brief...

List the four phases of mitosis, and briefly explain what occurs during each phase. Prophase: the chromatin coils and produces chromosomes, the nucleolus and nuclear envelope d

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd