Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Antibodies
Antibodies are important.tools for detecting and localising specific molecules in the cells due to their high specificity. The first requirement for this is to produce antibodies against a specific molecule. Suppose you want to produce antibodies against tubulin, the purified tubulin is first obtained from a given tissue. It is then injected into an animal such as rabbit which in nwse to tubulin produces antibodies. Blood sample is collected from the animal and antibodies are isolated. These antibodies are used to locakise the specific molecule in the cell because they bind only 80 an antigen, which is a tubulia in this case. Localisation can be done by two methods: direct and inditect In the direct method, antibodies are first labelled with a flouroscendt dye-like rhodamide for light microscopy, or witha high molecular weight, electron dense compound like ferritin for transmission electron microscopy. Fluorescent labelled antibodies are allowed to react with cells so as to form systematic antibody complex. The complex is then examined undera microscope (fluorescent microscope or TEM). The specific molecules will emit light or become electron dense due to their binding with labelled antibodies (Figure shown below). This method is used to locdise foreign bodies such us viruses, bacterial antigevs, etc.
The major ecological imbalance on Earth is between the human species and the rest of Earth's biota. What are two specific actions we could do to achieve a balance (for example, rec
comments on acutus
classification schemes and characterisitics used for this.
Determine the term - Language Abilities The study of aphasia and acquired language disorders was one of the driving forces in the growth of neuropsychology in this century. Th
The movement of Na + and glucose from the lumen of the intestine across the epithelial cell to the blood sets up a dissimilarity in osmotic pressure across the cell. As
P r o c esse d Chicken Products Preparation of comminuted meat products is the best way to salvage meat from spent hens and broiler parent stock. Incorporation of whole eg
Explain Precautions for Determination of Fungal and Yeast Count? 1. Maintain aseptic conditions. 2. Keep alcohol away from flame. 3. To avoid infections and allergies, ca
Results of Monohybrid Crosses Mendel got the following results from his monohybrid crosses :- 1. F 1 plants produced by a cross between two plants pure for the cont
High blood pressure (hypertension) has gained considerable significance because it will eventually damage end organs like the heart, brain, kidneys and the eyes. It is estimated th
MITOCHONDRIA It is the power house of the cell because they are the major centers of release of energy in the aerobic respiration. Mitochondria and chloroplast both are a
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd