Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Antibodies
Antibodies are important.tools for detecting and localising specific molecules in the cells due to their high specificity. The first requirement for this is to produce antibodies against a specific molecule. Suppose you want to produce antibodies against tubulin, the purified tubulin is first obtained from a given tissue. It is then injected into an animal such as rabbit which in nwse to tubulin produces antibodies. Blood sample is collected from the animal and antibodies are isolated. These antibodies are used to locakise the specific molecule in the cell because they bind only 80 an antigen, which is a tubulia in this case. Localisation can be done by two methods: direct and inditect In the direct method, antibodies are first labelled with a flouroscendt dye-like rhodamide for light microscopy, or witha high molecular weight, electron dense compound like ferritin for transmission electron microscopy. Fluorescent labelled antibodies are allowed to react with cells so as to form systematic antibody complex. The complex is then examined undera microscope (fluorescent microscope or TEM). The specific molecules will emit light or become electron dense due to their binding with labelled antibodies (Figure shown below). This method is used to locdise foreign bodies such us viruses, bacterial antigevs, etc.
Define role of Vitamin A in controlling gene expression? Vitamin A as retinoic acid has widespread effects on the synthesis of a variety of important proteins. These diverse ef
Explain the Recommended Dietary Allowance for nicotinic acid? There are various factors and which niacin requirements depend. These are energy Utilization, body size and dietar
Role of Government in Health Care Provision The government intervenes in the health sector with a general view of improving the health status of the people. In general governm
#what is naturalistic theory?
The pumps The blood from the operating field is sucked with the help of the cardiotomy sucker and pump, which is filtered to remove all particulate matter and then pass
BON E MARROW TRANSPLANT - It is transplanted to treat leukemia. Here, graft itself causes immune rejection. Before transplant, the recipient's bone marrow cells, incl
Concept behind Freezing the milk Freezing the milk also alters its composition and properties to a great extent. Let us learn about these changes. As the milk freezes, it becom
Which is the biological molecule that contains the genetic information that is transmitted hereditarily and controls the cellular functioning? The hereditary molecule that cont
Define Procedure for Detection of Chalk in Flour Carry out the experiment following the procedure given herewith: 1. Take a teaspoon of flour in a test tube and shake it wit
What is genotype? What is the difference between phenotype and genotype? The Genotype is the genes, DNA nucleotide sequences contained in the chromosomes of an individual, whic
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd