Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Antibodies
Antibodies are important.tools for detecting and localising specific molecules in the cells due to their high specificity. The first requirement for this is to produce antibodies against a specific molecule. Suppose you want to produce antibodies against tubulin, the purified tubulin is first obtained from a given tissue. It is then injected into an animal such as rabbit which in nwse to tubulin produces antibodies. Blood sample is collected from the animal and antibodies are isolated. These antibodies are used to locakise the specific molecule in the cell because they bind only 80 an antigen, which is a tubulia in this case. Localisation can be done by two methods: direct and inditect In the direct method, antibodies are first labelled with a flouroscendt dye-like rhodamide for light microscopy, or witha high molecular weight, electron dense compound like ferritin for transmission electron microscopy. Fluorescent labelled antibodies are allowed to react with cells so as to form systematic antibody complex. The complex is then examined undera microscope (fluorescent microscope or TEM). The specific molecules will emit light or become electron dense due to their binding with labelled antibodies (Figure shown below). This method is used to locdise foreign bodies such us viruses, bacterial antigevs, etc.
Q. Bone Loss - criteria for endosteal implants? Crestal bone loss after intial healing is a primary indicator of the need for initial preventive therapy. Early loss of crestal
In an investigation to compare the rates at which starch is being broken down by an enzyme (a) what test is used (b) how do you know when the reaction
How Water content Influence the RS content? The yield of RS formed in heat-moisture treatment is closely related to water content, which may be an inherent component of food or
Describe about Aorto Pulmonary Window ? This uncommon malformation consists of communication usually non-restrictive between the adjacent walls of the ascending aorta and pulmo
which area of the basal nuclei is responsible for controlling appendicular muscle tone?
What is the difference between brain and cerebrum? What are the main parts of these structures? The concept of brain, or encephalon, comprehends the cerebrum (mostly referred t
H Y ALURONI C ACID It is formed by alternating unit of D-glucuronic acid and N-acetylglucosamine. Hyaluronic acid acts as animal cement between adjacent animal cells
#queshition..
Q. Which kind of circulatory system do molluscs have? How can it be compared to the respiratory system of annelids? Molluscs, with the exception of cephalopods, have an open ci
Infectious proteins are present in: 1.Gemini viruses 2.Prions 3.Viroids 4.Satellite viruses Prions
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd