Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Antibodies
Antibodies are important.tools for detecting and localising specific molecules in the cells due to their high specificity. The first requirement for this is to produce antibodies against a specific molecule. Suppose you want to produce antibodies against tubulin, the purified tubulin is first obtained from a given tissue. It is then injected into an animal such as rabbit which in nwse to tubulin produces antibodies. Blood sample is collected from the animal and antibodies are isolated. These antibodies are used to locakise the specific molecule in the cell because they bind only 80 an antigen, which is a tubulia in this case. Localisation can be done by two methods: direct and inditect In the direct method, antibodies are first labelled with a flouroscendt dye-like rhodamide for light microscopy, or witha high molecular weight, electron dense compound like ferritin for transmission electron microscopy. Fluorescent labelled antibodies are allowed to react with cells so as to form systematic antibody complex. The complex is then examined undera microscope (fluorescent microscope or TEM). The specific molecules will emit light or become electron dense due to their binding with labelled antibodies (Figure shown below). This method is used to locdise foreign bodies such us viruses, bacterial antigevs, etc.
Explain the Classification of Malignant Tumors Malignant tumors can be classified as: Carcinomas: These tumors arise from the epithelial lining (the squamous cells) e.g. ski
Reduction of NADP+ occurs during what?
Explain the Executive Functions - Psychological tests Executive functions are those involved in the planning, organisation, regulation and monitoring of goal directed behaviour
Explain Scope of Food Science and Technology as a Subject? Food science and technology has developed as a discipline to systematically organize and link various kinds of knowle
Explain Functional property of WaterBinding/ Hydration Mode of action Proteins can help reduce formula costs as the proteins hold additional water. Food system
Define Specialized Nutritional Formulations for CHD? Achieving calorie and protein requirements with restricted fluid intake, you would realize, is a formidable challenge. Hig
Optic vesicle We have explained earlier that the presumptive material for the optic vesicles lies in qe eye field in the anterior region of the early neural plate. Experiments
Organizational assessments are vital to the introduction of Nurse Case Management (NCM) and Clinical Nurse Leaders (CNL) ( I am in school for the CNL) Use the guidelines below
Submerged implants Decision has to be made between two techniques. These techniques are: Technique 1: Stage two using a mucoperiosteal flap Technique 2: Stage two done wi
COMBINE D EFFECTS OF DRUGS AND ALCOHOL - 1. Alcohol + Barbiturates → may lead to death . 2. Alcohol + Antihistamines → Drowsiness 3. Alcohol + Valium
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd