Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Antibodies
Antibodies are important.tools for detecting and localising specific molecules in the cells due to their high specificity. The first requirement for this is to produce antibodies against a specific molecule. Suppose you want to produce antibodies against tubulin, the purified tubulin is first obtained from a given tissue. It is then injected into an animal such as rabbit which in nwse to tubulin produces antibodies. Blood sample is collected from the animal and antibodies are isolated. These antibodies are used to locakise the specific molecule in the cell because they bind only 80 an antigen, which is a tubulia in this case. Localisation can be done by two methods: direct and inditect In the direct method, antibodies are first labelled with a flouroscendt dye-like rhodamide for light microscopy, or witha high molecular weight, electron dense compound like ferritin for transmission electron microscopy. Fluorescent labelled antibodies are allowed to react with cells so as to form systematic antibody complex. The complex is then examined undera microscope (fluorescent microscope or TEM). The specific molecules will emit light or become electron dense due to their binding with labelled antibodies (Figure shown below). This method is used to locdise foreign bodies such us viruses, bacterial antigevs, etc.
What happens to respiratory system in a man going up a hill?
Explain Basal Metabolism Rate (BMR) - Ageing? From age 25 years, the basal metabolism decreases by about 2 percent for each decade due to the increasing proportion of body fat
Studying a single rock Select a single rock and try to learn as more as possible about it from careful observation. If it is flat, it is probably a piece or layer from Sediment
PHYSIOLOGY OF EXCRETION - 1 . FORMATION OF UREA - It takes place in liver. (i) Deamination (ii) Ornithine cycle or urea cycle - Explained by H.
How does a sickle cell carrier have a benefit over the rest of the human population in a malaria ridden area? Why is organ transplantation from another person generally unsucces
Lipid Digestion Digestion of fats is also similar in both invertebrates and vertebrates. Lipases are the - enzymes that hydrolyse fats. A single lipase can catalyse many step
Controlling Introduction of Alien Species As you know the alien species can cause the existing population to decrease or even become extinct. The individuals of alien species
Disability Adjusted Life Years (DALYs) DALYs is a measure for the overall ‘burden of disease’. Originally developed by the World Health Organization (WHO), it is being increas
Motor Control in Annelids and Arthropods In annelids and arthropods generally, individual metameric ganglia of the ventral nerve cord are capable of initiating and keep locomo
Q. Dietary recommendations proposed by WHOM? We learnt about their etiological factors, metabolic alterations, clinical anifestations and dietary management of the disease of h
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd