Antibodies, Biology

Assignment Help:

Antibodies

Antibodies are important.tools for detecting and localising specific molecules in the cells due to their high specificity. The first requirement for this is to produce antibodies against a specific molecule. Suppose you want to produce antibodies against tubulin, the purified tubulin is first obtained from a given tissue. It is then injected into an animal such as rabbit which in nwse to tubulin produces antibodies. Blood sample is collected from the animal and antibodies are isolated. These antibodies are used to locakise the specific molecule in the cell because they bind only 80 an antigen, which is a tubulia in this case. Localisation can be done by two methods: direct and inditect

In the direct method, antibodies are first labelled with a flouroscendt dye-like rhodamide for light microscopy, or witha high molecular weight, electron dense compound like ferritin for transmission electron microscopy. Fluorescent labelled antibodies are allowed to react with cells so as to form systematic antibody complex. The complex is then examined under
a microscope (fluorescent microscope or TEM). The specific molecules will emit light or become electron dense due to their binding with labelled antibodies (Figure shown below). This method is used to locdise foreign bodies such us viruses, bacterial antigevs, etc.

618_antibodies.jpg


Related Discussions:- Antibodies

Voluntary movements of the big toe of the right foot, Which of the followin...

Which of the following is true for a toe corticospinal interneuron that produces action potentials during voluntary movements of the big toe of the right foot? A. Its dendrites

Difference between ectoparasite and endoparasite, What is the difference be...

What is the difference between ectoparasite and endoparasite? Ectoparasites are parasites that explore the external surface of the host (as, for example, mites that parasite th

Explain food habits - diet presciption, Food Habits Food Habits: These ...

Food Habits Food Habits: These must be known  so that the diets  can  be  recommended keeping in mind the  food preferences  of  the patient. Whether a person is  a vegetarian

Living organisms, List the five kingdoms into which most living organisms c...

List the five kingdoms into which most living organisms can be classified. Most living organisms can be classified as a) Bacteria, b) Protozoa, c) Fungi, d) Plants,

., what is nuclus

what is nuclus

Diseases, what are the sources of infection (human, animal and non-living r...

what are the sources of infection (human, animal and non-living reservoirs), the routes of transmission (way in which the infection spreads) and different ways in which micro-organ

What are pioneer species, What are pioneer species? What is the role of the...

What are pioneer species? What is the role of the pioneer species? Pioneer species are those first species that colonize places where before there were no living beings, like,

Determine fat-soluble vitamins recommended for infant, Determine fat-solubl...

Determine fat-soluble vitamins Recommended for Infant? ICMR (1990) does not give recommendations for vitamins D, E and K. As you already know, vitamin D is expected to be met

How to dna fragments for comparison with other dna samples, DNA fingerprint...

DNA fingerprinting produces a unique banding pattern of DNA fragments for comparison with other DNA samples.

Define microscopy and physiological analysis, Dr. Herbert discovers a cell ...

Dr. Herbert discovers a cell and he is convinced it is a plant cell. However, as a student of his genetics class, you do not agree with him. You do not believe in microscopy and ph

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd