Antibodies, Biology

Assignment Help:

Antibodies

Antibodies are important.tools for detecting and localising specific molecules in the cells due to their high specificity. The first requirement for this is to produce antibodies against a specific molecule. Suppose you want to produce antibodies against tubulin, the purified tubulin is first obtained from a given tissue. It is then injected into an animal such as rabbit which in nwse to tubulin produces antibodies. Blood sample is collected from the animal and antibodies are isolated. These antibodies are used to locakise the specific molecule in the cell because they bind only 80 an antigen, which is a tubulia in this case. Localisation can be done by two methods: direct and inditect

In the direct method, antibodies are first labelled with a flouroscendt dye-like rhodamide for light microscopy, or witha high molecular weight, electron dense compound like ferritin for transmission electron microscopy. Fluorescent labelled antibodies are allowed to react with cells so as to form systematic antibody complex. The complex is then examined under
a microscope (fluorescent microscope or TEM). The specific molecules will emit light or become electron dense due to their binding with labelled antibodies (Figure shown below). This method is used to locdise foreign bodies such us viruses, bacterial antigevs, etc.

618_antibodies.jpg


Related Discussions:- Antibodies

Define about the classification of polyphenols, Define about the Classifica...

Define about the Classification of polyphenols?  The various classes are: a) Phenolic acids and derivatives b) Flavonoids such as Flavonols and Flavones, Isoflavones and

Respiration in cockroach, what is the chemical reaction of respiration in c...

what is the chemical reaction of respiration in cockroach?

What mechanisms would na and water be secreted, When William was helping vi...

When William was helping victims following a devastating earthquake in a region that was not prepared to swiftly set up adequate temporary shelter, he developed severe diarrhea. He

Conditions associated with increased need for vitamin a, Define Conditions ...

Define Conditions associated with increased need for Vitamin A? Conditions and populations associated with increased need for vitamin A includes young children  particularly  t

Meiosis and Mitosis, #question.Consider a cell that has 10 homologous pairs...

#question.Consider a cell that has 10 homologous pairs of chromosomes and is just starting to go through meiosis. After meiosis is complete, there will be 40 cells that each contai

Evaluation of disabilities, Problem: Evaluation of disabilities due to ...

Problem: Evaluation of disabilities due to occupational diseases (a) Describe the following terms: "impairment", "disability "and "handicap" (b) Show how the evaluation o

Define corneal xerosis - micronutrient deficiencies, Define Corneal Xerosis...

Define Corneal Xerosis - Micronutrient Deficiencies? This is a sign of severe vitamin 'A' deficiency, in which the cornea loses its normal smooth and glistening appearance and

Explain in details amino acid, Explain in details Amino Acid? Amino Ac...

Explain in details Amino Acid? Amino Acids :   There are 20 common amino acids, with a few more found in lower organisms. Adult humans require a diet that includes at least 8

What is reflexive memory, What is reflexive memory? Reflexive memory re...

What is reflexive memory? Reflexive memory relies on the cerebellum and amygdala. Compare among aerobic respiration and anaerobic respiration. Aerobic respiration happens in th

Defects in human development, Defects in Human Development 1) For inst...

Defects in Human Development 1) For instance, when a pregnant woman drinks alcohol her embryo is exposed to similar concentration of alcohol as is in her blood. The alcohol ma

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd