Antibodies, Biology

Assignment Help:

Antibodies

Antibodies are important.tools for detecting and localising specific molecules in the cells due to their high specificity. The first requirement for this is to produce antibodies against a specific molecule. Suppose you want to produce antibodies against tubulin, the purified tubulin is first obtained from a given tissue. It is then injected into an animal such as rabbit which in nwse to tubulin produces antibodies. Blood sample is collected from the animal and antibodies are isolated. These antibodies are used to locakise the specific molecule in the cell because they bind only 80 an antigen, which is a tubulia in this case. Localisation can be done by two methods: direct and inditect

In the direct method, antibodies are first labelled with a flouroscendt dye-like rhodamide for light microscopy, or witha high molecular weight, electron dense compound like ferritin for transmission electron microscopy. Fluorescent labelled antibodies are allowed to react with cells so as to form systematic antibody complex. The complex is then examined under
a microscope (fluorescent microscope or TEM). The specific molecules will emit light or become electron dense due to their binding with labelled antibodies (Figure shown below). This method is used to locdise foreign bodies such us viruses, bacterial antigevs, etc.

618_antibodies.jpg


Related Discussions:- Antibodies

What is gingival inflammation, What is Gingival Inflammation Areas of o...

What is Gingival Inflammation Areas of overt inflammation should be identified and the etiology investigated to initiate proper treatment.

Biologists established that new cells, Biologists established that new cell...

Biologists established that new cells Consequent to this doctrine, biologists established that new cells are formed by cell division and by the end of the nineteenth century, t

Define reagents required and methodology for fehling's test, Define Reagent...

Define Reagents required and Methodology for Fehling's Test? - Sugar solutions of glucose, fructose, galactose, lactose, maltose, sucrose, starch - Fehling A (Copper sulphat

Enzyme-linked receptors, Numerous receptors have intrinsic or tightly relat...

Numerous receptors have intrinsic or tightly related enzyme activity.  On the required of ligand to their extracellular face like receptors undergo a conformational change and acti

Ecology, what is ecology?sub divison of ecology?

what is ecology?sub divison of ecology?

Determine the major demographic processes, Determine the major demographic ...

Determine the major demographic processes? There are five major demographic processes, which are continually at work within the population namely, fertility, mortality, marriag

What is the function of the flagellum of the sperm cell, Q. What is the fun...

Q. What is the function of the flagellum of the sperm cell? How is it formed? The flagellum of the sperm cell is made by the centrioles that migrate to the region posterior to

Eggs of hen, EGG S OF HEN It is a meiolecithal egg which is oval. T...

EGG S OF HEN It is a meiolecithal egg which is oval. The animal pole is found in the form of a small germinal disc. The vegital pole has two types of yolk viz. White yol

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd