Antibodies, Biology

Assignment Help:

Antibodies

Antibodies are important.tools for detecting and localising specific molecules in the cells due to their high specificity. The first requirement for this is to produce antibodies against a specific molecule. Suppose you want to produce antibodies against tubulin, the purified tubulin is first obtained from a given tissue. It is then injected into an animal such as rabbit which in nwse to tubulin produces antibodies. Blood sample is collected from the animal and antibodies are isolated. These antibodies are used to locakise the specific molecule in the cell because they bind only 80 an antigen, which is a tubulia in this case. Localisation can be done by two methods: direct and inditect

In the direct method, antibodies are first labelled with a flouroscendt dye-like rhodamide for light microscopy, or witha high molecular weight, electron dense compound like ferritin for transmission electron microscopy. Fluorescent labelled antibodies are allowed to react with cells so as to form systematic antibody complex. The complex is then examined under
a microscope (fluorescent microscope or TEM). The specific molecules will emit light or become electron dense due to their binding with labelled antibodies (Figure shown below). This method is used to locdise foreign bodies such us viruses, bacterial antigevs, etc.

618_antibodies.jpg


Related Discussions:- Antibodies

Define human digestive system, Q. Which organs of the body is part of the h...

Q. Which organs of the body is part of the human digestive system? The digestive system also known as "systema digestorium" or gastrointestinal system is composed of the digest

What is resting membrane potential of an axon, Action potentials include ch...

Action potentials include changes in the membrane potential of an axon, as shown in the schematic below. During the refractory period, sodium channels are inactivated and

Define absorption, Define Absorption, Storage and Elimination of Vitamin D?...

Define Absorption, Storage and Elimination of Vitamin D? As we have already mentioned earlier, all fat-soluble vitamins share a common metabolic fate. Vitamin D is absorbed al

Energy storage, Energy Storage As we said above, food intake and ener...

Energy Storage As we said above, food intake and energy expenditure for animals is approximately equal. If energy expenditure exceeds food intake, then the excess energy is t

Reduction in the birth rate, State three conditions in a society which coul...

State three conditions in a society which could contribute to a reduction in the birth rate. Birth rate might be decreased by (i) later marriages (as smaller families), (

What are some prophylactic measures against hiv infection, What are some pr...

What are some prophylactic measures against HIV infection? The major prophylactic measures against HIV infection are: the use of condoms in sexual relations, not to share syrin

Scrapie, Scrapie This is a chronic fatal disease of sheep and occasiona...

Scrapie This is a chronic fatal disease of sheep and occasionally of goats caused by a Prion agent similar to BSE. The disease is reported from Europe, North America and in a f

What concentration of sucrose solution should administered, The volume of M...

The volume of Mrs. X's plasma was 3 liters before Ed administered the IV. Assume that there was a complete mixing of the administered water with her plasma but no mixing with her i

Six kingdom worksheet, what protist changes shape constantly and flows arou...

what protist changes shape constantly and flows around its food to engulf it?

Into which two groups can the nitrogen-containing bases, Into which two gro...

Into which two groups can the nitrogen-containing bases that form DNA and RNA be classified? What is the criterion used in that classification? The nitrogen-containing bases th

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd