Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Antibodies
Antibodies are important.tools for detecting and localising specific molecules in the cells due to their high specificity. The first requirement for this is to produce antibodies against a specific molecule. Suppose you want to produce antibodies against tubulin, the purified tubulin is first obtained from a given tissue. It is then injected into an animal such as rabbit which in nwse to tubulin produces antibodies. Blood sample is collected from the animal and antibodies are isolated. These antibodies are used to locakise the specific molecule in the cell because they bind only 80 an antigen, which is a tubulia in this case. Localisation can be done by two methods: direct and inditect In the direct method, antibodies are first labelled with a flouroscendt dye-like rhodamide for light microscopy, or witha high molecular weight, electron dense compound like ferritin for transmission electron microscopy. Fluorescent labelled antibodies are allowed to react with cells so as to form systematic antibody complex. The complex is then examined undera microscope (fluorescent microscope or TEM). The specific molecules will emit light or become electron dense due to their binding with labelled antibodies (Figure shown below). This method is used to locdise foreign bodies such us viruses, bacterial antigevs, etc.
Determine the term - Nitrogen Most of nitrogen is available as organic nitrogen which has to be mineralised to inorganic form to become available to plants. Estimation of orga
Mention where the following are located in the human brain, and give single function of each: a) Temporal lobe b) Cerebellum c) Corpus callosum
Define Mineral Absorption? NDOs affect mainly calcium/magnesium absorption and balance. Acidification of colonic contents increases the concentration of ionized minerals, parti
Valvular Heart Diseases Normal heart valves function to maintain a uni-directional flow of blood through cardiac chambers. Two basic problems that compromise the normal functi
Q. Carbohydrates requirement in chronic diarrhoea? Adequate amount of carbohydrates i. e 60-65% of the total energy should be given to the patient. Easily assimilated carbohydr
Q. What is Short Bowel Syndrome? Short bowel syndrome is a group of problems affecting people who have /had half or more of their small intestine removed. The massive resection
explain chemical changes during cleavage
Suppose that you are raising Downton wombats, carefully because they bite when teased.You notice that some of your wombats have white spots, whereas others have solid coats. What t
These are found in about 3-6 per cent of cases of ARF. These nodules are typically subcutaneous, firm, painless, freely movable (0.5-2 cm size) and their presence indicates that t
Polarity in Regeneration A distinct polarity or gradient exists in planarians (and in other animals). The meaning of this is that in any piece or segment of the body; its ant
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd