Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Antibodies
Antibodies are important.tools for detecting and localising specific molecules in the cells due to their high specificity. The first requirement for this is to produce antibodies against a specific molecule. Suppose you want to produce antibodies against tubulin, the purified tubulin is first obtained from a given tissue. It is then injected into an animal such as rabbit which in nwse to tubulin produces antibodies. Blood sample is collected from the animal and antibodies are isolated. These antibodies are used to locakise the specific molecule in the cell because they bind only 80 an antigen, which is a tubulia in this case. Localisation can be done by two methods: direct and inditect In the direct method, antibodies are first labelled with a flouroscendt dye-like rhodamide for light microscopy, or witha high molecular weight, electron dense compound like ferritin for transmission electron microscopy. Fluorescent labelled antibodies are allowed to react with cells so as to form systematic antibody complex. The complex is then examined undera microscope (fluorescent microscope or TEM). The specific molecules will emit light or become electron dense due to their binding with labelled antibodies (Figure shown below). This method is used to locdise foreign bodies such us viruses, bacterial antigevs, etc.
Hexokinase, that catalyzes the first irreversible step of glycolysis, is inhibited by glucose 6-phosphate. Thus when PFK is inhibited and fructose 6-phosphate builds up an
Explain the Postoperative Nutritional Care? The therapeutic goal for the post-operative patient is rapid recovery to normal function and well being, minimum complications and e
Q. What are mycotoxins and How are they harmful? • Mycotoxins are toxins (secondary metabolites) produced by filamentous fungi on food and feed that cause illness when ingested
Epithelial cells of the kidney proximal tubule ? A. The sodium-glucose co-transporter in the luminal membrane is responsible for the net flux of glucose from luminal space to
Explain about Urinary system Urinary system provides us with the service of disposing the waste products carried by blood. This function is also known as excretion.
Q. Observation of Hemichordata ? • identify Balanoglossus as a hemicliordate and give its scientific and common name. • classify Balanogiossus LIP to the level of order.
What are the main constituents of the earth's atmosphere in our time? The present atmosphere of the earth is constituted essentially of molecular nitrogen (N2) and molecular ox
commercially important bivalve species
Q. Predominant feature of steatorrhoea? A predominant feature is delayed and defective absorption of fat, which results in bulky stools containing large quantities of fat (know
Explain Skeletal muscle Skeletal muscle has low activity of glucose-6-phosphate dehydogenase. Yet, like most other tissues it can synthesize ribose-5-phosphate. This is.
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd