Antibodies, Biology

Assignment Help:

Antibodies

Antibodies are important.tools for detecting and localising specific molecules in the cells due to their high specificity. The first requirement for this is to produce antibodies against a specific molecule. Suppose you want to produce antibodies against tubulin, the purified tubulin is first obtained from a given tissue. It is then injected into an animal such as rabbit which in nwse to tubulin produces antibodies. Blood sample is collected from the animal and antibodies are isolated. These antibodies are used to locakise the specific molecule in the cell because they bind only 80 an antigen, which is a tubulia in this case. Localisation can be done by two methods: direct and inditect

In the direct method, antibodies are first labelled with a flouroscendt dye-like rhodamide for light microscopy, or witha high molecular weight, electron dense compound like ferritin for transmission electron microscopy. Fluorescent labelled antibodies are allowed to react with cells so as to form systematic antibody complex. The complex is then examined under
a microscope (fluorescent microscope or TEM). The specific molecules will emit light or become electron dense due to their binding with labelled antibodies (Figure shown below). This method is used to locdise foreign bodies such us viruses, bacterial antigevs, etc.

618_antibodies.jpg


Related Discussions:- Antibodies

Free-hand sectioning of plant tissue (stem), What are the processes involve...

What are the processes involved in the preparation of plant tissue for free hand sectioning?

What is the genetic disease in which this factor is absent, What is factor ...

What is factor VIII? What is the genetic disease in which this factor is absent? Factor VIII has the function of activating factor X that in its turn is essential for the trans

Biological species concept, Q. Biological species concept? A biological...

Q. Biological species concept? A biological species as defined by Ernst Mayr are "groups of actually or potentially interbreeding natural populations which are reproductively

Scintigraphy or nuclear medicine, S c inti g r ap h y ...

S c inti g r ap h y or nuclear medicine: Scintigraphy was introduced in veterinary medicine in 1970s. It is highly sensitive and can detect bone dama

Healthcare delivery - risk pooling, Normal 0 false false fa...

Normal 0 false false false EN-IN X-NONE X-NONE MicrosoftInternetExplorer4

What are the features of hnrna, Q. What are the features of hnRNA? Hete...

Q. What are the features of hnRNA? Heterogeneous nuclear RNA (hnRNA) - Primary precursor mRNA transcript made in eukaryotic nucleus are called 'hnRNA,' an abbreviation for 'het

Venous drainage of the heart, The coronary sinus is the main vein of the he...

The coronary sinus is the main vein of the heart and is about 3 cm. long. It lies in the coronary sulcus at the posterior surface of  the heart in the posterior atrio-ventricular g

Illustrate about the neuropsychological battery, NEUROPSYCHOLOGICAL BATTERY...

NEUROPSYCHOLOGICAL BATTERY The battery was published in 1980 by Western Psychological Services and is now extensively used in clinical and research applications. An alternate

Explain process of rice milling, Rice milling Rice milling involves the...

Rice milling Rice milling involves the following processing steps: rough rice (paddy rice) → hull removal  →  brown rice  → polishing to remove the bran coats (fruits and seed

Explain adverse effects of stavudine, Explain Adverse effects of Stavudine ...

Explain Adverse effects of Stavudine Fatal lactic acidosis may happen more frequently with stavudine than with other NRTIs. Serum aminotransferase activity may enhance with sta

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd