Antibodies, Biology

Assignment Help:

Antibodies

Antibodies are important.tools for detecting and localising specific molecules in the cells due to their high specificity. The first requirement for this is to produce antibodies against a specific molecule. Suppose you want to produce antibodies against tubulin, the purified tubulin is first obtained from a given tissue. It is then injected into an animal such as rabbit which in nwse to tubulin produces antibodies. Blood sample is collected from the animal and antibodies are isolated. These antibodies are used to locakise the specific molecule in the cell because they bind only 80 an antigen, which is a tubulia in this case. Localisation can be done by two methods: direct and inditect

In the direct method, antibodies are first labelled with a flouroscendt dye-like rhodamide for light microscopy, or witha high molecular weight, electron dense compound like ferritin for transmission electron microscopy. Fluorescent labelled antibodies are allowed to react with cells so as to form systematic antibody complex. The complex is then examined under
a microscope (fluorescent microscope or TEM). The specific molecules will emit light or become electron dense due to their binding with labelled antibodies (Figure shown below). This method is used to locdise foreign bodies such us viruses, bacterial antigevs, etc.

618_antibodies.jpg


Related Discussions:- Antibodies

What are the differences between minerals and ores , Menerals are naturally...

Menerals are naturally occurring inorganic solids with a crystalline structure and a definite range of chemical formula. Ores are concentrations of minerals in rock.

Population distribution, Population Distribution - Population Parameters an...

Population Distribution - Population Parameters and Regulation Dispersion or distribution refers to the pattern of distribution of individuals of a population. As shown in Fig

Blood flow during exercise - circulation, Normal 0 false fals...

Normal 0 false false false EN-IN X-NONE X-NONE MicrosoftInternetExplorer4

Which chemical causes tooth destruction, The bacteria that cause dental cav...

The bacteria that cause dental cavities in humans break down sugars, releasing what chemical which causes tooth destruction? a) Acids b) Bases c) Enzymes d) Monosaccha

Molybdenum and vanadium, Molybdenum and Vanadium - Inorganic Nitrogen and S...

Molybdenum and Vanadium - Inorganic Nitrogen and Sulphur Metabolism Molybdenum must be available in nature in order to meet the demand of Mo for the formation of nitrogenase n

Vasa diffrentia, VAS A DIFFRENTIA - Originate from wollfian duct ....

VAS A DIFFRENTIA - Originate from wollfian duct . It comes out fo cauda epdidymis enters into abdominal cavity through inguinal canal, forms loop round the uretor and j

What are the merits of the closed circulatory system, Q. What are the merit...

Q. What are the merits of the closed circulatory system over the open circulatory system? The closed circulatory system is more resourceful since blood circulates only inside b

How to evaluate blanching efficiency, How to evaluate blanching efficiency?...

How to evaluate blanching efficiency? Finally, let us learn how to evaluate blanching efficiency? Normally, two of the more heat resistant plant enzymes, namely peroxidase and

Etiologic factor of atherosclerosis, Q. Etiologic factor of atherosclerosis...

Q. Etiologic factor of atherosclerosis? Various factors are responsible for atherosclerosis. These include: 1. Hyperlipidemin: Excess circulating fats in blood especially th

What is excessive cantilever, Excessive Cantilever Cantilevers in impla...

Excessive Cantilever Cantilevers in implant dentistry are commonly used especially in the mandibular arch for edentulous patients receiving implant supported prosthesis. Cantil

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd