Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Antibodies
Antibodies are important.tools for detecting and localising specific molecules in the cells due to their high specificity. The first requirement for this is to produce antibodies against a specific molecule. Suppose you want to produce antibodies against tubulin, the purified tubulin is first obtained from a given tissue. It is then injected into an animal such as rabbit which in nwse to tubulin produces antibodies. Blood sample is collected from the animal and antibodies are isolated. These antibodies are used to locakise the specific molecule in the cell because they bind only 80 an antigen, which is a tubulia in this case. Localisation can be done by two methods: direct and inditect In the direct method, antibodies are first labelled with a flouroscendt dye-like rhodamide for light microscopy, or witha high molecular weight, electron dense compound like ferritin for transmission electron microscopy. Fluorescent labelled antibodies are allowed to react with cells so as to form systematic antibody complex. The complex is then examined undera microscope (fluorescent microscope or TEM). The specific molecules will emit light or become electron dense due to their binding with labelled antibodies (Figure shown below). This method is used to locdise foreign bodies such us viruses, bacterial antigevs, etc.
Q What are some examples of tissues and organs where mitosis is more frequent, less frequent or practically absent? Generally in vertebrates mitosis is more frequent in tissues
Determine the type of bandage The most common type of bandage is the gauze bandage, a simple woven strip of material which comes in different widths and lengths.
The fundamental contractile unit is the sarcomere. Each muscle cell apart from the nucleus, mitochondria and other structures contains parallel fibrils. Sarcomere is a subunit of t
State the term - Molybdenum Molybdenum is intimately related to nitrogen metabolism of plants. It is part of the nitrate reductase system which helps to utilise nitrate for n
Q. Do the membranes form only the outer wrapping of cells? Lipid membranes do not form only the outer cover of cells. Cell organelles, such as the Golgi complex, lysosomes, chl
REMOVAL OF TESTIS - It is male hypogonadism. (1 ) BEFORE PUBERTY - Cestration before puberty results in permanent sterility. This condition is eunuchoidism & shows the
Effect on Water Bodies - Dissolved Oxygen (DO) Most aquatic, organisms respire with oxygen dissolved in water. The quantity of dissolved oxygen in a unit volume of aerated wa
Functionality of implant material It should take maximum advantage of available bone and permit the maximum amount of forces to be transmitted through the implant within physio
Do plants placed under an environment drier than the habitat where they are used to living have a reduction or an increase in the time during which their stomata remain open? I
Explain Transport and Utilization of Vitamin A? The efficacy of the intestines to facilitate absorption and utilization of retinoid s and carotenoids depends upon the cellular
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd