Antibodies, Biology

Assignment Help:

Antibodies

Antibodies are important.tools for detecting and localising specific molecules in the cells due to their high specificity. The first requirement for this is to produce antibodies against a specific molecule. Suppose you want to produce antibodies against tubulin, the purified tubulin is first obtained from a given tissue. It is then injected into an animal such as rabbit which in nwse to tubulin produces antibodies. Blood sample is collected from the animal and antibodies are isolated. These antibodies are used to locakise the specific molecule in the cell because they bind only 80 an antigen, which is a tubulia in this case. Localisation can be done by two methods: direct and inditect

In the direct method, antibodies are first labelled with a flouroscendt dye-like rhodamide for light microscopy, or witha high molecular weight, electron dense compound like ferritin for transmission electron microscopy. Fluorescent labelled antibodies are allowed to react with cells so as to form systematic antibody complex. The complex is then examined under
a microscope (fluorescent microscope or TEM). The specific molecules will emit light or become electron dense due to their binding with labelled antibodies (Figure shown below). This method is used to locdise foreign bodies such us viruses, bacterial antigevs, etc.

618_antibodies.jpg


Related Discussions:- Antibodies

Skin., what is the difference between fair skin and dark skin?

what is the difference between fair skin and dark skin?

Taxonomy.., what is the androcium in china rose ..

what is the androcium in china rose ..

What are the three main types of trophic pyramids, What are the three main ...

What are the three main types of trophic pyramids studied in Ecology? The three types of trophic pyramids studied in Ecology are the numeric pyramid, the biomass pyramid and th

Why does the ingestion of alcohol increase diuresis, Q. Why does the ingest...

Q. Why does the ingestion of alcohol increase diuresis? Alcohol inhibits the secretion of ADH that is antidiuretic hormone by the pituitary that is why when it is drunk to exce

Explain briefly Creutzfeldt-jakob disease (cjd), Normal 0 false...

Normal 0 false false false EN-US X-NONE X-NONE MicrosoftInternetExplorer4

Excretion in reptiles, describe the procedure of excretion in reptiles

describe the procedure of excretion in reptiles

Protozoa, Advantages and disadvantages of protozoa

Advantages and disadvantages of protozoa

What is muller manoeuver explain in details, What is Muller Manoeuver expla...

What is Muller Manoeuver explain in details? In this manoeuver, patient forcibly inspires while the nose is held closed and mouth firmly sealed for 10 seconds. It exaggerates t

Number of risk factors of gout, Q. Number of risk factors of gout? A nu...

Q. Number of risk factors of gout? A number of risk factors are related to the development of hyperuricemia and gout. These factors include: • Hereditary: Genetics may play

Biological fixation - nitrogen fixation, Biological fixation - Nitrogen Fix...

Biological fixation - Nitrogen Fixation Approximately 63% of all nitrogen fixed is through biological fixation. Nitrogen fixing organisms are primarily prokaryotes; bacteria a

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd