Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Antibodies
Antibodies are important.tools for detecting and localising specific molecules in the cells due to their high specificity. The first requirement for this is to produce antibodies against a specific molecule. Suppose you want to produce antibodies against tubulin, the purified tubulin is first obtained from a given tissue. It is then injected into an animal such as rabbit which in nwse to tubulin produces antibodies. Blood sample is collected from the animal and antibodies are isolated. These antibodies are used to locakise the specific molecule in the cell because they bind only 80 an antigen, which is a tubulia in this case. Localisation can be done by two methods: direct and inditect In the direct method, antibodies are first labelled with a flouroscendt dye-like rhodamide for light microscopy, or witha high molecular weight, electron dense compound like ferritin for transmission electron microscopy. Fluorescent labelled antibodies are allowed to react with cells so as to form systematic antibody complex. The complex is then examined undera microscope (fluorescent microscope or TEM). The specific molecules will emit light or become electron dense due to their binding with labelled antibodies (Figure shown below). This method is used to locdise foreign bodies such us viruses, bacterial antigevs, etc.
Phosphorus cycle is sedimentary cycle because it does not enter into atmosphere. It can be found on earth in water, soil and sediments. The phosphorus cycle is the biogeochemical c
Enantiomers: Select one: a. Have the same molecular weight b. Have the same connectivity c. Are mirror images d. Are non-superimposable e. All of the above
The acidity and alkalinity (pH) of an environment has a strong influence on the activity and stability of macromolecules such as enzymes. These enzymes play an important role durin
Archaeological Records of Human Campsites Archaeological records of human campsites indicate that early humans started out as small bands of hunters who supplemented kills wit
Define Sex as a determinants of nutrient requirements? There are some differences in requirements between the two sexes. However, except for iron, these are apparent rather tha
Define Important Points While Working With Autoclave? Note: Following points should be kept in mind while working with autoclave. 1. Autoclave should not be packed tightly o
Community Gradients and Boundaries It is often difficult or impossible to determine where one community ends and the next begins. Many communities, in fact, grade continuousl
What is gene? Gene is a sequence of DNA nucleotides that codifies the production of a protein.
Can someone explain how is cancer related to ras and p53 gene? I know that the ras protein regulate cell growth and its mutation will cause cancer. How is the mutation caused?
How is HIV transmitted? What is the disease caused by this virus? HIV (human immunodeficiency virus) is supposed to be transmitted by blood, semen, maternal milk and vaginal se
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd