Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Antibodies
Antibodies are important.tools for detecting and localising specific molecules in the cells due to their high specificity. The first requirement for this is to produce antibodies against a specific molecule. Suppose you want to produce antibodies against tubulin, the purified tubulin is first obtained from a given tissue. It is then injected into an animal such as rabbit which in nwse to tubulin produces antibodies. Blood sample is collected from the animal and antibodies are isolated. These antibodies are used to locakise the specific molecule in the cell because they bind only 80 an antigen, which is a tubulia in this case. Localisation can be done by two methods: direct and inditect In the direct method, antibodies are first labelled with a flouroscendt dye-like rhodamide for light microscopy, or witha high molecular weight, electron dense compound like ferritin for transmission electron microscopy. Fluorescent labelled antibodies are allowed to react with cells so as to form systematic antibody complex. The complex is then examined undera microscope (fluorescent microscope or TEM). The specific molecules will emit light or become electron dense due to their binding with labelled antibodies (Figure shown below). This method is used to locdise foreign bodies such us viruses, bacterial antigevs, etc.
What is the Still Retorts? Still Retorts are used in canning plants for heating foods in glass containers or different sizes of cans. These retorts are usually cylindrical in s
How can the excretory system of annelids be described? In each segment (metamere) of the being a pair of complete excretory structures called metanephridium exists. The metanep
what is tay-sachs diseases in human beings as mendal incomplete dominance f2 genration
Define Function of thiamin as Regulator of enzyme activity? Thiamin regulates the enzymes involved in carbohydrate metabolism. These are: a) Pyruvate dehydrogenase, which pr
mode of digestion in coelenterata
what is plasmodium
Q. According to their functions how can nutrients are classified? One possible and utile functional classification for nutrients is the one that separates them into energetic,
Q. What are the venous of the valves system? What is their function? The valves of the venous system are structures inside the veins that permit blood to flow only in the norma
Explain Environmental Sampling - Methods and Techniques? Environmental sampling can be done for total microbial load or for some specific pathogens or spoilage organisms. The m
Adverse effects of Lactic acidosis- Lactic acidosis and severe hepatomegaly with steatosis have been reported with use of other nucleoside analogs. The most common adverse effe
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd