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Antibodies
Antibodies are important.tools for detecting and localising specific molecules in the cells due to their high specificity. The first requirement for this is to produce antibodies against a specific molecule. Suppose you want to produce antibodies against tubulin, the purified tubulin is first obtained from a given tissue. It is then injected into an animal such as rabbit which in nwse to tubulin produces antibodies. Blood sample is collected from the animal and antibodies are isolated. These antibodies are used to locakise the specific molecule in the cell because they bind only 80 an antigen, which is a tubulia in this case. Localisation can be done by two methods: direct and inditect In the direct method, antibodies are first labelled with a flouroscendt dye-like rhodamide for light microscopy, or witha high molecular weight, electron dense compound like ferritin for transmission electron microscopy. Fluorescent labelled antibodies are allowed to react with cells so as to form systematic antibody complex. The complex is then examined undera microscope (fluorescent microscope or TEM). The specific molecules will emit light or become electron dense due to their binding with labelled antibodies (Figure shown below). This method is used to locdise foreign bodies such us viruses, bacterial antigevs, etc.
What is the Bio-medical waste The Bio-medical waste (Management & Handling Rules), 1998 is applicable to all persons, who generate, collect, receive, store, transport, treat,
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Ask question importance of genetics in human life #Minimum 100 words accepted#
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Which of the following structures in a vertebrate with a four-chambered heart would have blood with the highest oxygen concentration? And why? A. Arteriole end of a capillary B. Ri
DIVERSITY OF AMPHIBIANS ASSIGNMET IN BIOLOGY PLWASE HELP ME TO DO THIS ASSIGNMENT AND I CAN GET GOOD MARKS IN THIS ASSIGNMENT SO GIVE ME TNE ASSIFNMENT OF THIS TOPIC DIVERSITY OF A
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