Antibodies, Biology

Assignment Help:

Antibodies

Antibodies are important.tools for detecting and localising specific molecules in the cells due to their high specificity. The first requirement for this is to produce antibodies against a specific molecule. Suppose you want to produce antibodies against tubulin, the purified tubulin is first obtained from a given tissue. It is then injected into an animal such as rabbit which in nwse to tubulin produces antibodies. Blood sample is collected from the animal and antibodies are isolated. These antibodies are used to locakise the specific molecule in the cell because they bind only 80 an antigen, which is a tubulia in this case. Localisation can be done by two methods: direct and inditect

In the direct method, antibodies are first labelled with a flouroscendt dye-like rhodamide for light microscopy, or witha high molecular weight, electron dense compound like ferritin for transmission electron microscopy. Fluorescent labelled antibodies are allowed to react with cells so as to form systematic antibody complex. The complex is then examined under
a microscope (fluorescent microscope or TEM). The specific molecules will emit light or become electron dense due to their binding with labelled antibodies (Figure shown below). This method is used to locdise foreign bodies such us viruses, bacterial antigevs, etc.

618_antibodies.jpg


Related Discussions:- Antibodies

Define tests for the presence of exoenzymatic activity, Define Tests for th...

Define Tests for the Presence of Exoenzymatic Activity? Microorganisms require various micro- and macro-nutrients for energy production and growth. These are obtained from the

What are the allosteric enzymes, Q. What are the allosteric enzymes? Al...

Q. What are the allosteric enzymes? Allosteric enzymes are those that have in excess of one activation center and to which other substances, called allosteric regulators, bind.

What are integral membrane proteins, Membrane proteins are classify as eith...

Membrane proteins are classify as either integral (intrinsic) or peripheral (extrinsic) depending on how tightly they are linked with membrane. The Integral membrane proteins are s

How can amine groups be classified, How can amine groups be classified? ...

How can amine groups be classified? Amines can be classify into primary amines, those to which one -R (variable radical) is attached to a -NH2, secondary amines, those where on

Explain atrial switch operation, Explain Atrial Switch Operation ? The ...

Explain Atrial Switch Operation ? The hospital mortality reported varies between 0 and 15 per cent. Late survival is worse for TGA with VSD compared to simple TGA. 15 year surv

Bovine viral diarrhoea, B o v i ne viral diarrhoea Bovine viral dia...

B o v i ne viral diarrhoea Bovine viral diarrhoea (BVD) and mucosal disease (MD) are clinically dissimilar disease syndrome yet have a common viral etiology. The acute dise

Describe cardiac rewabilptation and its various effects, Describe cardiac r...

Describe cardiac rewabilptation and its various effects ? A survival benefit cannot be attributed solely to exercise training because many studies involved multifunctional inte

What is the relationship between these cells and hiv, Q. What are CD4 lymph...

Q. What are CD4 lymphocytes? What is the relationship between these cells and HIV? How does HIV replicate? The CD4 lymphocytes are T helper lymphocytes that present in their pl

Describe the phylum echinodermata in details, Describe the Phylum Echinoder...

Describe the Phylum Echinodermata in details? The Phylum Echinodermata takes its name from two words of Greek origin: "echino," ; which means "prickle" or "spine," and "derma,"

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd