Antibodies, Biology

Assignment Help:

Antibodies

Antibodies are important.tools for detecting and localising specific molecules in the cells due to their high specificity. The first requirement for this is to produce antibodies against a specific molecule. Suppose you want to produce antibodies against tubulin, the purified tubulin is first obtained from a given tissue. It is then injected into an animal such as rabbit which in nwse to tubulin produces antibodies. Blood sample is collected from the animal and antibodies are isolated. These antibodies are used to locakise the specific molecule in the cell because they bind only 80 an antigen, which is a tubulia in this case. Localisation can be done by two methods: direct and inditect

In the direct method, antibodies are first labelled with a flouroscendt dye-like rhodamide for light microscopy, or witha high molecular weight, electron dense compound like ferritin for transmission electron microscopy. Fluorescent labelled antibodies are allowed to react with cells so as to form systematic antibody complex. The complex is then examined under
a microscope (fluorescent microscope or TEM). The specific molecules will emit light or become electron dense due to their binding with labelled antibodies (Figure shown below). This method is used to locdise foreign bodies such us viruses, bacterial antigevs, etc.

618_antibodies.jpg


Related Discussions:- Antibodies

How did the industrial revolution in england, How did the industrial revolu...

How did the industrial revolution in England offer an example of natural selection? One of the classic instances of natural selection is regarding the moths of industrial zones

Will argon tend to form bonds with other elements, The atomic number of arg...

The atomic number of argon is 18. Will argon tend to form bonds with other elements? Describe your answer. Argon will not tend to form bonds with other elements. With an atomi

Entomophily - cross-pollination, Entomophily - Cross-pollination It in...

Entomophily - Cross-pollination It involves insects to carry the pollen to achieve pollination. Salvia exhibits a specialised 'tumapipe' floral mechanism that signifies classi

Circulatory system, Circulatory System Coelenterates and platyhelminth...

Circulatory System Coelenterates and platyhelminths nutrients in sponges, respiratory gases and waste materials can easily diffuse through the intercellular spaces. Though, in

Circulatory system in cockroach, CIRCUL A T O R Y SYSTEM IN COCKROACH ...

CIRCUL A T O R Y SYSTEM IN COCKROACH Type is open or lacunar. i.e. b.v. absent. Organs are dipped in haemolymph. 1 .      HAEMOLYMPH - Blood and lymph are mixed.

Ameoba, locomotion in amoeba

locomotion in amoeba

Determine recommended nutrient intakes by selenium, Determine recommended n...

Determine recommended nutrient intakes by selenium? The FAO/WHO 2004 recommendation for nutrient intake for selenium by groups is given in Table. How do these recommendations c

Write short notes on urine, Write short notes on Urine? The concentrate...

Write short notes on Urine? The concentrated filtrate produced by the kidney is called urine, and is composed mainly of water, urea, toxins, and mineral salts. Urine from the c

Describe any three adaptations of mangroves, Name and describe any three ad...

Name and describe any three adaptations of mangroves to the conditions prevailing in the Sunderbans.

Demonstration of tremendous diversity, Q. Demonstration of tremendous diver...

Q. Demonstration of tremendous diversity? A objective of taxonomy is the assemblage of knowledge gained. This is usually in the form of treatises useful to fellow scientists an

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd