Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Antibodies
Antibodies are important.tools for detecting and localising specific molecules in the cells due to their high specificity. The first requirement for this is to produce antibodies against a specific molecule. Suppose you want to produce antibodies against tubulin, the purified tubulin is first obtained from a given tissue. It is then injected into an animal such as rabbit which in nwse to tubulin produces antibodies. Blood sample is collected from the animal and antibodies are isolated. These antibodies are used to locakise the specific molecule in the cell because they bind only 80 an antigen, which is a tubulia in this case. Localisation can be done by two methods: direct and inditect In the direct method, antibodies are first labelled with a flouroscendt dye-like rhodamide for light microscopy, or witha high molecular weight, electron dense compound like ferritin for transmission electron microscopy. Fluorescent labelled antibodies are allowed to react with cells so as to form systematic antibody complex. The complex is then examined undera microscope (fluorescent microscope or TEM). The specific molecules will emit light or become electron dense due to their binding with labelled antibodies (Figure shown below). This method is used to locdise foreign bodies such us viruses, bacterial antigevs, etc.
Gluconeogenesis synthesizes glucose from noncarbohydrate precursors, involving pyruvate and lactate, citric acid cycle intermediates, the carbon skeletons of most glycerol and amin
Are viruses cellular organisms?
Human disorders are increasingly being diagnosed and treated using biotechnological applications such as: Genetic testing, containing testing of adults by to pre-birth diag
Pathophysiology of Acute Renal Failure It is usually reversible but the deviation of physiologic function can be extreme and mortality in pediatric age group remains high. Th
J o h n e ' s disease It is also known as paratuberculosis characterized by chronic enteritis and progressive weakness in dairy animals. E t iology: Mycobac
Why is knowledge of chemistry necessary for the study of living organisms?
There are several different lipoprotein species found in plasma but their basic structures are similar. The insoluble lipid (cholesterol ester and triglyceride) forms a central cor
Extraradicular Infections Whatever the cause of post endodontic disease we should do proper diagnosis to determine what is the cause; is it intra or extraradicular infection ,
Infection Due to the many invasive monitoring techniques, ET tube, urinary catheter, surgical interventions. Cultures of blood or sputum/urine/swab form the wound is don
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd