Antibodies, Biology

Assignment Help:

Antibodies

Antibodies are important.tools for detecting and localising specific molecules in the cells due to their high specificity. The first requirement for this is to produce antibodies against a specific molecule. Suppose you want to produce antibodies against tubulin, the purified tubulin is first obtained from a given tissue. It is then injected into an animal such as rabbit which in nwse to tubulin produces antibodies. Blood sample is collected from the animal and antibodies are isolated. These antibodies are used to locakise the specific molecule in the cell because they bind only 80 an antigen, which is a tubulia in this case. Localisation can be done by two methods: direct and inditect

In the direct method, antibodies are first labelled with a flouroscendt dye-like rhodamide for light microscopy, or witha high molecular weight, electron dense compound like ferritin for transmission electron microscopy. Fluorescent labelled antibodies are allowed to react with cells so as to form systematic antibody complex. The complex is then examined under
a microscope (fluorescent microscope or TEM). The specific molecules will emit light or become electron dense due to their binding with labelled antibodies (Figure shown below). This method is used to locdise foreign bodies such us viruses, bacterial antigevs, etc.

618_antibodies.jpg


Related Discussions:- Antibodies

Respiratory organ, . which is the main part of respiratory organ?

. which is the main part of respiratory organ?

Transcription in eukaryotes, The Eukaryotic transcription is more difficult...

The Eukaryotic transcription is more difficult than prokaryotic transcription. For example, in eukaryotes the genetic material DNA and therefore transcription is basically localize

Safety, S a f e ty, ethical and welfare considerations Development ...

S a f e ty, ethical and welfare considerations Development of transgenic organisms is proceeding in an environment of intense public debate about safety, environmental impa

Explain mental changes - clinical signs of kwashiorkor, Explain Mental chan...

Explain Mental changes - clinical signs of kwashiorkor? You would find a kwashiorkor child to be unusually apathetic with absolutely no interest in the surroundings. The child

Class turbellaria, plz answer me how I arrange my topic with its order ?

plz answer me how I arrange my topic with its order ?

Complement system, Complement system is a chemical defense system which ki...

Complement system is a chemical defense system which kills the microorganisms directly, it supplements inflammatory response, and works with, or complements, the immune system.

Explain marine oils, Marine oils These oils typically contain large amo...

Marine oils These oils typically contain large amounts of long - chain omega-3-polyunsaturated fatty acids, with up to six double bonds and they are usually rich in vitamins A

Microfilaments - role of cytoskeleton structures, Microfilaments - Role of ...

Microfilaments - Role of Cytoskeleton Structures Microfilaments are as well present in all animal cells. They are found singly throughout the cytoplasm organized like a meshwo

Agro industrial-biological evaluation of feedstuff, Biological evaluation ...

Biological evaluation A small livestock unit if attached with a mill will be of immense help. Since inspite analyzing for various mycotoxins still we face some problems, at lea

Define the calcium toxicity, Define the Calcium Toxicity? Elevated bloo...

Define the Calcium Toxicity? Elevated blood calcium can occur in association with high parathyroid hormone, hyper- or hypothyroid conditions, bone metastasis, vitamin D toxicit

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd