Antibodies, Biology

Assignment Help:

Antibodies

Antibodies are important.tools for detecting and localising specific molecules in the cells due to their high specificity. The first requirement for this is to produce antibodies against a specific molecule. Suppose you want to produce antibodies against tubulin, the purified tubulin is first obtained from a given tissue. It is then injected into an animal such as rabbit which in nwse to tubulin produces antibodies. Blood sample is collected from the animal and antibodies are isolated. These antibodies are used to locakise the specific molecule in the cell because they bind only 80 an antigen, which is a tubulia in this case. Localisation can be done by two methods: direct and inditect

In the direct method, antibodies are first labelled with a flouroscendt dye-like rhodamide for light microscopy, or witha high molecular weight, electron dense compound like ferritin for transmission electron microscopy. Fluorescent labelled antibodies are allowed to react with cells so as to form systematic antibody complex. The complex is then examined under
a microscope (fluorescent microscope or TEM). The specific molecules will emit light or become electron dense due to their binding with labelled antibodies (Figure shown below). This method is used to locdise foreign bodies such us viruses, bacterial antigevs, etc.

618_antibodies.jpg


Related Discussions:- Antibodies

Explain nutritional effects of hemicelluloses, Nutritional effects Of Hemic...

Nutritional effects Of Hemicelluloses Hemicelluloses are classified under the category of soluble dietary fibers.  Hemicellulose is an indigestible complex carbohydrate that ab

Ilustrate the binding of atp to actin, Which of the following is true in a ...

Which of the following is true in a skeletal muscle? A. Movement of the cross-bridge occurs only when the myosin head is detached from the actin molecule. B. The binding of

Disadvantage of a monoculture, (a)What do you understand by the term 'monoc...

(a)What do you understand by the term 'monoculture'? (b) What is one disadvantage of a monoculture?   a) 'Monoculture' is the term applied to the growing

Illustrate morphological evidence, Q. Illustrate Morphological Evidence? ...

Q. Illustrate Morphological Evidence? Morphology is the study of structure and form of plants and animals usually dealing with the organism and its component organs. Morphologi

Role of echocardiography, Echocardiography is a very important tool in diag...

Echocardiography is a very important tool in diagnosis of cardiovascular diseases and is used  virtually in all categories of cardiovascular diseases. There are certain areas where

Determine the three types of joints, Determine the three types of joints ...

Determine the three types of joints There are three types of joints : Fixed or immovable or fibrous : There is no space between the bones. The attached bones are very ti

Define briefly about the copper, Define Briefly about the Copper? As ea...

Define Briefly about the Copper? As early as the times of Hippocrates, copper compounds were used to treat various diseases. However, in the 20" century, it was noticed that an

Explain subphylum mandibulata, Subphylum Mandibulata Most of them have ...

Subphylum Mandibulata Most of them have three pairs of walking legs, mandibles, compound eyes, antennas, some with wings. There are four classes. Crustacea - aquatic with

Why the physician suspect, Kevin and his wife have been trying to have a ba...

Kevin and his wife have been trying to have a baby for the past three years. On seeking the help of a fertility specialist, Kevin learned that he has a hereditary form of male ster

Explain about monocot plants and dicot plants, What are the major morpholog...

What are the major morphological differences between monocot plants and dicot plants? The main differentiation criteria among monocots and dicots are: number of cotyledons (see

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd