Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Antibodies
Antibodies are important.tools for detecting and localising specific molecules in the cells due to their high specificity. The first requirement for this is to produce antibodies against a specific molecule. Suppose you want to produce antibodies against tubulin, the purified tubulin is first obtained from a given tissue. It is then injected into an animal such as rabbit which in nwse to tubulin produces antibodies. Blood sample is collected from the animal and antibodies are isolated. These antibodies are used to locakise the specific molecule in the cell because they bind only 80 an antigen, which is a tubulia in this case. Localisation can be done by two methods: direct and inditect In the direct method, antibodies are first labelled with a flouroscendt dye-like rhodamide for light microscopy, or witha high molecular weight, electron dense compound like ferritin for transmission electron microscopy. Fluorescent labelled antibodies are allowed to react with cells so as to form systematic antibody complex. The complex is then examined undera microscope (fluorescent microscope or TEM). The specific molecules will emit light or become electron dense due to their binding with labelled antibodies (Figure shown below). This method is used to locdise foreign bodies such us viruses, bacterial antigevs, etc.
How are the epithelial tissues classified? The epithelial tissues are classified according to the shape of the cells that form it (epithelial cells may be cuboidal, columnar, o
Write about 25 parasites of protozoans? And writ the class family and full history about these parasites?
Define Vapor-pressure lowering and Osmotic pressure? 1. Vapor-pressure lowering: the decrease in the vapor pressure of a solution containing nonvolatile solutes, compared to th
Cell culture products: Animal cell cultures are used to generate virus vaccines, as well as different kind of useful biochemical which are mainly high molecular weight prote
ADOLESCENC E - It is the period of growth from the onset of puberty to the completion of growth & maturity. It extends between 12- 20 yrs. It is a period of rapid physical
Control of Pollution We have seen that pollution affects human health, 'vegetation, crop yield, trees, fisheries, forests, buildings, archaeological monuments, tourism. These
SPECIE S CONCEPT - According to Davis and Heywood species define as assemblage of individuals with morphological features in common and separable from other such assembla
what direct or indirect effect is " acid rain" thought to have on (I) lakes, (ii) forests and (iii) buildings
If the titration is done by adding NaOH to a solution containing 2.661 moles of lysine, how many milliliters (mL) of a 5 M solution of NaOH would you have to add to reach point "E"
How can the presence, localization and function of muscular tissue in beings of the phylum Annelida be explained? In these beings there are a longitudinal muscular layer under
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd