Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Antibodies
Antibodies are important.tools for detecting and localising specific molecules in the cells due to their high specificity. The first requirement for this is to produce antibodies against a specific molecule. Suppose you want to produce antibodies against tubulin, the purified tubulin is first obtained from a given tissue. It is then injected into an animal such as rabbit which in nwse to tubulin produces antibodies. Blood sample is collected from the animal and antibodies are isolated. These antibodies are used to locakise the specific molecule in the cell because they bind only 80 an antigen, which is a tubulia in this case. Localisation can be done by two methods: direct and inditect In the direct method, antibodies are first labelled with a flouroscendt dye-like rhodamide for light microscopy, or witha high molecular weight, electron dense compound like ferritin for transmission electron microscopy. Fluorescent labelled antibodies are allowed to react with cells so as to form systematic antibody complex. The complex is then examined undera microscope (fluorescent microscope or TEM). The specific molecules will emit light or become electron dense due to their binding with labelled antibodies (Figure shown below). This method is used to locdise foreign bodies such us viruses, bacterial antigevs, etc.
Rehabilitation Phase for nutritional treatment - Neuro Trauma? Once the patient stabilizes and starts recovering, be sides nutritional replacement, there is a need to assess fu
What are the fruiting bodies present in some fungi? Fruiting bodies are structures made of hyphae that project radially from the superior portion of the peduncle of some fungi.
Parts of a Seed Seed is attached to the fruit by a stalk, the funiculus (funicle). The prolongation of the funiculus running along the seed and terminating at the chalaza is
Q. Dietary Guidelines fur hyperlipidemic patients? • Calories: to maintain ideal body weight. • Carbohydrates should constitute 55-65% of calories with emphasis on • polysacc
What is katal katal : the amount of enzyme that transforms 1 mol of substrate into product in one second.
Q. Bacteria - type of microorganisms? Many of us know bacteria only as "germs," invisible to naked eyes that can invade our bodies and make us sick. Few know that many bacteria
Mitochondria are the organelles in which the most significant part of the cellular respiration happens: the ATP production.
E mb ryonic stem cells (ES): These are pleuripotent cells of early embryo (blastocyst) capable of giving rise to all differentiated cells, including germ line cells. Em
What is Passive Transport? Passive Transport : Specialized proteins called permeases also move specific substances across the cell membrane in a process called carrier transp
Determine the food sources of riboflavin? The food sources of riboflavin include: 1. Rich sources: Liver, dried yeast, egg powder, milk powder. 2. Good sources: Who
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd