Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Antibodies
Antibodies are important.tools for detecting and localising specific molecules in the cells due to their high specificity. The first requirement for this is to produce antibodies against a specific molecule. Suppose you want to produce antibodies against tubulin, the purified tubulin is first obtained from a given tissue. It is then injected into an animal such as rabbit which in nwse to tubulin produces antibodies. Blood sample is collected from the animal and antibodies are isolated. These antibodies are used to locakise the specific molecule in the cell because they bind only 80 an antigen, which is a tubulia in this case. Localisation can be done by two methods: direct and inditect In the direct method, antibodies are first labelled with a flouroscendt dye-like rhodamide for light microscopy, or witha high molecular weight, electron dense compound like ferritin for transmission electron microscopy. Fluorescent labelled antibodies are allowed to react with cells so as to form systematic antibody complex. The complex is then examined undera microscope (fluorescent microscope or TEM). The specific molecules will emit light or become electron dense due to their binding with labelled antibodies (Figure shown below). This method is used to locdise foreign bodies such us viruses, bacterial antigevs, etc.
Q. Living beings are made of inorganic and organic substances. According to the molecular complexity how can each of those substances be classified? Inorganic substances, mole
ADRENAL OR SUPRARENAL GLANDS (GLANDS OF EMERGENCY) - These are paired structures located on the top of the kidneys. Each adrenal gland has two parts external adrenal cort
Q. What are the factors which influence the spoilage of meat? Can you list a few? Ans. Sure enough, you should be able to enumerate these having learnt about them earlier
Difference between Reproduction System of man and rabbit - S.No. Man Rabbit 1. 2. 3.
Q. What are the Physical Signs of mitral stenosis? A typical malar flush is described in mitral stenosis. It is difficult to appreciate in Indians. Pulse is normal or low volum
Definition of Somatoform Disorders: The term 'Somatoform Disorders' was introduced in the scientific literature by the American Psychiatric Association in the third edition o
Explain colloidal state Foods contain a high percentage of water in which other nutrients present are dispersed. The existence of the colloidal state was first recognized by T
Q. What is the concept of universality of the genetic code? What are the exceptions to this universality? The genetic code is universal because the rules of protein codificatio
Explain the Nerve Cell Structure and Function? Before studying the organization of the nervous system, we will look at the relationship between structure and function of nerve
Q. What do you mean by hydraulic modelling? Modelling is neither new nor relevant to hydraulic engineering only. It is practiced in the field of hydraulic machinery for testing
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd