Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Antibodies
Antibodies are important.tools for detecting and localising specific molecules in the cells due to their high specificity. The first requirement for this is to produce antibodies against a specific molecule. Suppose you want to produce antibodies against tubulin, the purified tubulin is first obtained from a given tissue. It is then injected into an animal such as rabbit which in nwse to tubulin produces antibodies. Blood sample is collected from the animal and antibodies are isolated. These antibodies are used to locakise the specific molecule in the cell because they bind only 80 an antigen, which is a tubulia in this case. Localisation can be done by two methods: direct and inditect In the direct method, antibodies are first labelled with a flouroscendt dye-like rhodamide for light microscopy, or witha high molecular weight, electron dense compound like ferritin for transmission electron microscopy. Fluorescent labelled antibodies are allowed to react with cells so as to form systematic antibody complex. The complex is then examined undera microscope (fluorescent microscope or TEM). The specific molecules will emit light or become electron dense due to their binding with labelled antibodies (Figure shown below). This method is used to locdise foreign bodies such us viruses, bacterial antigevs, etc.
A wire 50.0 m long and 2.00 mm in diameter is connected to a source with a potential difference of 9.11 V, and the current is found to be 33.7 A. Assume a temperature of 20°C and,
Types of Transport Process in Sieve Tubes The metabolites of all the mesophyll cells around the sieve elements join in a common pool to load via the surrounding transfer cells
SEXUAL REPRODUCTION You must be aware that reproduction is one of the distinguishing features of living beings. Reproduction is basically of two types: asexual and sexual. In ase
Define e-PTFE suture material The e-PTFE suture material is a nonabsorbable monofilament that has high tensile strength, good handling properties, and good knot security, but
Q. Show the Natural selection of taxonomist? Natural selection associated with successful reproduction maintains a basic similarity of the reproductive feature of flowers, frui
A small family was traveling in the car when a minor accident happened. The children in the back seats were wearing lap belts, but still sustained numerous bruises about the abdome
What are coacervates? Coarcervates are small structures made of the aggregation of organic molecules under water solution. By electrical attraction the molecules join into bigg
Explain the Analysis of food production? The agricultural sector is known for its diversity and heterogeneity of decisions right from the farm to the entire marketing system. A
Morphological Changes - Floral Induction Some of the morphological changes that accompany floral induction are: Change in the shape of meristem, Increase in the ra
Blood functions to maintain homeostasis in the human body through all but which of the following: Answer moving carbon dioxide away from cells following completion of aerobic metab
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd