Antibodies, Biology

Assignment Help:

Antibodies

Antibodies are important.tools for detecting and localising specific molecules in the cells due to their high specificity. The first requirement for this is to produce antibodies against a specific molecule. Suppose you want to produce antibodies against tubulin, the purified tubulin is first obtained from a given tissue. It is then injected into an animal such as rabbit which in nwse to tubulin produces antibodies. Blood sample is collected from the animal and antibodies are isolated. These antibodies are used to locakise the specific molecule in the cell because they bind only 80 an antigen, which is a tubulia in this case. Localisation can be done by two methods: direct and inditect

In the direct method, antibodies are first labelled with a flouroscendt dye-like rhodamide for light microscopy, or witha high molecular weight, electron dense compound like ferritin for transmission electron microscopy. Fluorescent labelled antibodies are allowed to react with cells so as to form systematic antibody complex. The complex is then examined under
a microscope (fluorescent microscope or TEM). The specific molecules will emit light or become electron dense due to their binding with labelled antibodies (Figure shown below). This method is used to locdise foreign bodies such us viruses, bacterial antigevs, etc.

618_antibodies.jpg


Related Discussions:- Antibodies

Pollinia and massulae, Pollinia and Massulae In most cases the pollen ...

Pollinia and Massulae In most cases the pollen grains of each tetrad become separated from one another and they lie freely in the pollen sac. In some plants, mostly members of

Kingdom animalia, Locomotion and reproduction in echinodermata

Locomotion and reproduction in echinodermata

Types of fats, FATS ARE OF TWO TYPES (a) Hard Fats They are t...

FATS ARE OF TWO TYPES (a) Hard Fats They are true fats which are solid at room temperature of 20 o C. It is due to presence of long chain saturated fatty acid

Open and closed type of circulatory systems, Open and Closed Type of Circul...

Open and Closed Type of Circulatory Systems There are two categories of circulatory system found in higher metazoans. In one type the original blastocoel carries on to be the

EMBRYOLOGY., WHAT ARE THE TYPES OF EGG ON THE BASIS OF YOLK ?

WHAT ARE THE TYPES OF EGG ON THE BASIS OF YOLK ?

Explain noncompetitive inhibitor, Explain noncompetitive inhibitor A no...

Explain noncompetitive inhibitor A noncompetitive inhibitor can combine with either the free enzyme or the enzyme- substrate  complex, interfering  both.  The  most  common  ty

Dna replication, DNA Replication Deoxyribonucleic acid (DNA) is the car...

DNA Replication Deoxyribonucleic acid (DNA) is the carrier of genetic data for all living creatures. An organism's genome, made of DNA, encodes the genetic blueprint for buildi

Mouth examination of new born, Mouth Inspect and palpate the lips, ...

Mouth Inspect and palpate the lips, gums, tongue, palate and oropharynx. Rule out cleft lip and palate, size of chin if small and receding it  shows micrognathia ind

Diabetes mellitus, 1. How often should she have Glycated hemoglobin (HgbA1C...

1. How often should she have Glycated hemoglobin (HgbA1C) drawn?  What percentage is desired? 2. How often should she see an ophthalmologist or optometrist? 3. What should be

Etiologic factor of ulcerative colitis, Q. Etiologic factor of ulcerative c...

Q. Etiologic factor of ulcerative colitis? No single etiologic factor has been identified although genetic auto-immune factors are thought to be involved. Although exacerbation

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd