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Ammonification
Many heterotrophic bacteria actinomycetes and fungi in soil and water metabolise the organic nitrogen and release it in an inorganic form as ammonia. This process is known as ammonification or mineralisation. This is an energy releasing reaction. For example, glycine-based protein releases 176 kcal/mole. This energy is used to maintain the life 'process of the organisms that accomplish the transformation.
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What volume of 4x protein sample buffer do you need to add to 15 ml of a cell extract in order to load it on a gel?
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How many asymmetric carbons are there in ribose and glucose?
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