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Ammonification
Many heterotrophic bacteria actinomycetes and fungi in soil and water metabolise the organic nitrogen and release it in an inorganic form as ammonia. This process is known as ammonification or mineralisation. This is an energy releasing reaction. For example, glycine-based protein releases 176 kcal/mole. This energy is used to maintain the life 'process of the organisms that accomplish the transformation.
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Measurement of Nitrogenase Activity There are various methods to find out whether an organism is a N 2 -fixer or not. If an organism can grow in normal atmosphere without any
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