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Ammonia Assimilation - Inorganic Nitrogen and Sulphur Metabolism
Nitrogen (N2) gas and NO3 are the most common available forms of inorganic nitrogen. Both are enzymatically reduced to ammonia because it is only ammonia that is-incorporated into organic form. Since amino acids either free or part of proteins are the predominant form of organic nitrogen, the major product of ammonia assimilation is usually considered to be amino nitrogen.
Determination Reductive hydrolysis of folic acid produces 4-aminobenzoyl glutamic acid which is determined photometrically after diazotization and coupling with N-(1-naphthyl)
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