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The Amazon rainforest in South America is a biodiverse ecosystem. There are large numbers of plant and animal species making up the food web, including over 350 species of predatory insectivorous birds. In one small area of the rainforest, about 15 km2, there are 60 different species of Ithomiine butterflies.
These 60 species of butterflies have only eight different colour pattern phenotypes. Each of the different phenotypes is found within a particular habitat within this small area. Therefore the different phenotypes are rarely seen together. Scientists initially assumed that each of these phenotypes indicated that there were eight different species of butterflies. Genetic analysis has disproved this, and the resulting phylogeny is shown opposite. It shows, for example, that there are 11 species of butterfly with the ‘eurimedia' phenotype.
Evidence shows that each species of butterfly lays its eggs on only one variety of plant, which is different to other species of butterfly with the same phenotype found in their habitat. The caterpillars feed exclusively on this host plant. However, adult butterflies drink nectar from a range of different flowers which may be in common with other butterflies of the same phenotype. Butterflies sharing the same colour pattern display Mullerian and /or Batesian mimicry.
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This technique utilizes an enzyme resolvase, endo vii, cloned from the bacteriophage t4. This enzyme has high specificity to find deletions, insertions, and base substitutions muta
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