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Allele-specific ligation is the technique permitting discrimination of two allele at locus by giving two short synthetic oligonucleotides which would bind ajjacent to teach other on amplified DNA fragment, and would be ligated in the presence of DNA ligase; if one of alleles comprising mutation overlapped by 3'-end of one oligonucleotide, its ligation to oligonucleotide bound 3' to it would be prevented; to deduce the identity of the unknown allele, differentially labelled oligonucleotide pairs might be designed for each allele and their ligation efficiency compared in the presence of unknown allele.
Define Polyacrylamide gel electrophoresis (PAGE)? Acrylamide gel has the advantage over starch in that it is easier to prepare and is more inert, the pore size can be varied in
What is the mode of nutrition in fish,human,amoeba,scorpian & toad ?
A.N.S. (Autonimic Nervous System) Name given by Langley . Controled by Hypothelamus. Partially independent system. Controls involuntary actions as heart beat, breathing,
Q. What is tuberculosis? How is the disease transmitted? Is there treatment for tuberculosis? The Tuberculosis is a disease caused by the Mycobacterium tuberculosis bacteria wh
Precautions for Morphological Characteristics of Yeast Cells? 1. Observe aseptic conditions. 2. Use only a small sample for staining. 3. No air bubbles should be in the s
Benzathine penicillin given every three weeks as IM injection (1.2 mega units) has given the best results. Oral agents (penicillin V) are given in patients with lower risk of rheum
The atomic number of argon is 18. Will argon tend to form bonds with other elements? Describe your answer. Argon will not tend to form bonds with other elements. With an atomi
Hard paste removal - Hard setting Ca(Ho) 2 paste (like Endocal 10) can be softened by EDTA and removed by file or microdebrider. - Care must be taken not to gouge or
Which of the following serves as an actuating signal, or as part of an actuating signal, in a negative feedback system? A. Action potentials in diaphragm skeletal muscle fibers
How do sponges try to protect themselves against harm from the environment? Is that method efficient or rudimentary? Sponges can close their pores to avoid the entrance of wate
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