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Allele-specific ligation is the technique permitting discrimination of two allele at locus by giving two short synthetic oligonucleotides which would bind ajjacent to teach other on amplified DNA fragment, and would be ligated in the presence of DNA ligase; if one of alleles comprising mutation overlapped by 3'-end of one oligonucleotide, its ligation to oligonucleotide bound 3' to it would be prevented; to deduce the identity of the unknown allele, differentially labelled oligonucleotide pairs might be designed for each allele and their ligation efficiency compared in the presence of unknown allele.
What is the etiological agent and the main manifestations of cholera? Cholera is a bacterial disease caused by the Vibrio cholerae. The disease is transmitted by fecal-oral rou
Q. Determine the sucrose content in a sample of honey? This activity will help you to: • estimate the non-reducing sugars and total sugars in a sample of honey, • check t
how is water lost from respiratory surface
differences between acoelomate,coelomate and pseudocoelomate
Cross-Pollination - Types of Pollination In this kind of pollination the pollen from anther of one individual is transferred to the stigma of another individual of the same sp
Discuss mental nerve, its anatomic variation and surgical importance. Mental nerve, branch of inferior alveolar nerve is a very important landmark during osteotomy procedures.
Handling : The desire for survival is same whether in animals or human beings. You may be aware of the ability of even tiny animals to scratch and bite. So we won't say any more o
The male had a female first cousin (on his father side) who died from TSD and the female had a maternal uncle with TSD. Assume this trait is very rare. 1. Calculate the probabil
Two chromosomes in a cell have identical length and centromere position yet are not homologous, what is different about them.
Filaments of a sarcomere in a skeletal muscle When the overlap between the thin and thick filaments of a sarcomere in a skeletal muscle is decreasing, A. The total lengt
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