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Allele-specific ligation is the technique permitting discrimination of two allele at locus by giving two short synthetic oligonucleotides which would bind ajjacent to teach other on amplified DNA fragment, and would be ligated in the presence of DNA ligase; if one of alleles comprising mutation overlapped by 3'-end of one oligonucleotide, its ligation to oligonucleotide bound 3' to it would be prevented; to deduce the identity of the unknown allele, differentially labelled oligonucleotide pairs might be designed for each allele and their ligation efficiency compared in the presence of unknown allele.
Q. What are the major events of the third mitotic period? The third mitotic period is anaphase. In anaphase the following events occur breaking and duplication of centromeres w
what is the biological significance of the skeleton and supporting system in animals
Q. How DNA-RNA hybridization occurs Both DNA and RNA are able to form hybrids in solution with other RNA or DNA molecules which have complementary base pairing. Double-stranded
Minerals and Trace Elements Oxygen, carbon, hydrogen and nitrogen are the most common elements that make up 96% of the total weight of a mammal. The next most abundant element
Explain about the DNA viruses - Carcinogenic? The three DNA viruses found to cause human cancers are EBV, HBV and HPV. What are these? Let us find out. Epstein-Barr viru
Nursing Diagnosis Nursing diagnosis are judgements or conclusions reached by you after analyzing the data base that indicate a potential or actual human need that you as
Protein Classification Based on Shape There are three types of proteins on the basis of shape - Fibrous, Globular, Intermediate 1 . FIBROUS PROTEINS They ar
Conduits Figure: Venous Conduits Reversed saphenous vein was the first conduit used for CABG. 'It is usually harvested from the leg starting above the ankle going up
The overall goal of BCC programs for diabetes mellitus is to promote behaviors that control diabetes mellitus and prevent complications. These include: Following treatment
Q. What is ecological succession? The Ecological succession is the changing sequence of communities that live in an ecosystem during a given time period.
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