You want to make the buffer but you find you are out

Assignment Help Chemistry

Reference no: EM13635672

The most common buffer in mammalian tissue culture is aphosphate buffer. The three pKa's of phosphoric acid are 2.16,7.21, 12.32, respectively.

A) For cells to grow normally, the pH must be kept at thephysiological pH of 7.40. Starting with 100.0mL of 0.100M sodiumdihydrogen phosphate, how many mL of 0.100M NaOH must be added toget to physiological pH?

B) What are the concentrations of all species at physiologicalpH?

C) You want to make the buffer but you find you are out ofsodium dihydrogen phosphate so you must start with phosphoric acid.What is the pH of the solution if you add 1.524g of phosphoric acidto enough water to make 100.0mL of solution?

D) How much NaOH must you add to the solution from Part C toget to physiological pH? What is the new total volume?

E) Wouldn't you know it, when you go to the reagent shelf, youfind you are also out of phosphoric acid but at least there is somesodium phosphate. You add 1.882g of sodium phosphate to enoughwater to make 500.0mL of solution. What is the starting pH?

Reference no: EM13635672

Questions Cloud

Test the claim that the mean gpa of night students is : test the claim that the mean gpa of night students is smaller than the mean gpa of day students at the .05 significance

Test the claim that the proportion of men who own cats is : test the claim that the proportion of men who own cats is smaller than the proportion of women who own cats at the .10

Design the machine mounts consisting of a linear spring and : a machine of mass 20 kg is acted upon by a harmonic force having a maximum value of 25 n at a frequency of 120

A 15 m long string of mass 18 kg is streched such that the : a 15 m long string of mass 1.8 kg is streched such that the tension in the string is 25 n. a wave of frequency 20 hz is

You want to make the buffer but you find you are out : the most common buffer in mammalian tissue culture is aphosphate buffer. the three pkas of phosphoric acid are 2.167.21

Find the indicated probabilities using the geometric : find the indicated probabilities using the geometric distribution or the poisson distribution. then determine if the

A 05kg block at rest on a table is attached to a : a 0.5kg block at rest on a table is attached to a horizontal spring with a spring constant k200 nm. the spring is

Calculate the tension in the rope in newtons when you lift : your friend a 75 kg mountain climber is suspended from a cliff by a rope. calculate the tension in the rope in newtons

One biochemical reaction has a standard gibbs energy : one biochemical reaction has a standard gibbs energy of-200kjmol and a second biochemical reaction has a standard

User Account

All Pages