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bioinformatic You have been given a gene sequence, which is in RAW file format. Perform the following tasks by visually inspecting the sequence using information you were provided during lectures for Module 1, Topics 2 and 3. CCCTGGTTATTTATTGACAGGGGTATATAGTCCAGTGGTAGGCTGGATTA TATAATTTATTGAAATTTTCGATAAATTCCGTTATAATAATATTAAATCT GAAAATATTTAGTAAAAGGATGTGAGATATGTGAAGCTAAAACGCTTGTC TTTCTTCATGTTTGTTACCTTACTGGTATTTATATCTGTTTTTCCTGTTT ATGCAAACGATTTCGAAAAACATGGTACATATTATGAAATTTTTGTCAGG TCTTTTTATGACTCTGATGGTGACGGAATAGGGGATTTGAAAGGTATAAT AGAAAAACTGGATTATCTTAATGATGGAGACCCTGAAACCATTGCTGATC TGGGGGTTAATGGTATCTGGTTAATGCCTATCTTTAAATCTCCCTCCTAT CATGGCTATGATGTAACCGATTATTATAAGATTAATCCTGACTACGGGAC TCTGGAAGACTTCCATAAGCTTGTTGAGGCTGCCCATCAAAGGGGAATCA AGGTTATAATTGATTTACCCATCAATCATACCAGTGAAAGACATCCCTGG TTTCTCAAGGCTTCCCGGGATAAGAATAGTGAATACAGGGATTATTATGT CTGGGCTGGCCCCGATACCGATACCAAAGAAACCAAGTTAGATGGAGGCC GGGTCTGGCATTATTCCCCGACCGGCATGTATTATGGGTATTTCTGGAGT GGCATGCCTGATTTAAACTATAATAACCCTGAAGTTCAGGAAAAGGTTAT TGGGATAGCAAAATACTGGTTAAAACAGGGGGTTGATGGTTTCAGGCTTG ATGGAGCCATGCATATCTTCCCACCGGCCCAGTATGATAAAAACTTTACC TGGTGGGAGAAGTTCCGTCAGGAAATTGAAGAGGTAAAACCCGTTTACCT GGTGGGTGAGGTCTGGGATATTTCGGAAACGGTAGCTCCTTACTTCAAAT ATGGTTTTGATTCTACCTTTAACTTTAAACTGGCAGAGGCAGTTATCGCT ACGGCTAAAGCTGGATTTCCCTTTGGTTTTAATAAAAAGGCAAAACATAT TTACGGGGTATATGACAGGGAGGTTGGATTTGGGAATTATATCGATGCTC CCTTCCTGACCAACCATGATCAGAACCGGATTTTGGACCAGCTTGGGCAG GATCGTAATAAGGCCAGGGTTGCTGCCAGTATTTATTTGACCTTGCCTGG TAATCCCTTTATTTACTATGGTGAAGAAATCGGTATGAGGGGGCAGGGGC CCCATGAAGTTATCAGGGAGCCCTTCCAGTGGTATAATGGATCCGGGGAG GGAGAAACATACTGGGAGCCAGCCATGTATAATGATGGCTTTACTTCTGT TGAACAGGAAGAAAAGAATCTCGATTCCCTCTTAAATCACTACAGGAGGT TAATCCATTTCCGGAACGAAAATCCTGTCTTTTATACCGGTAAGATTGAG ATTATAAATGGAGGATTAAATGTAGTTGCATTTAGAAGATATAATGATAA GAGGGATTTATATGTCTACCATAACCTGGTAAACAGACCGGTTAAAATAA AAGTGGCTAGTGGTAACTGGACCTTATTGTTTAATTCAGGTGATAAGGAA ATTACCCCTGTTGAAGATAATAATAAACTTATGTATACTATCCCTGCTTA TACTACCATTGTTCTGGAAAAGGAGTAAAGGGAGAGGGTGAGTAATAATT ATGAAAAGGAGAGGGTAAATCCCTCTCCTTTTTTGTTAAA (a) Identify the possible Open Reading Frame (ORF). Hint: search for the START and STOP codons. (b) How many amino acids will the gene code for? (c) Translate the sequence and deduce the amino acid sequence for the putative protein. (d) Can you identify a signal peptide? If yes, identify it? (e) Sometimes, it is not possible to identify a gene using the method you used in question (a) above. One of the reasons is that the gene has not been sequenced completely - this happens during random sequencing of clones from bacterial genomic libraries. Perform a 6 frame translation of the given gene. Can you see the difference between the amino acid composition of all 6 translations. (f) Determine the G+C content of the coding region of the gene. Change the G+C content of the gene without changing the amino acid composition.
Explain plant life, animal life, and geology of the ecosystem in Utah. What populations and communities are present? How dependant is your community on this ecosystem? What are the preventive factors of the ecosystem.
Lipid metabolites are often seen as key elements in cellular signaling. Is this unique? Please provide several examples of the function of lipids as key elements in signal arrays and list the biologic functions these signals affect?
If you transform bacteria with the plasmid library made in part b above, divide the transformatnts into 20 aliquots and find one has the insert of interest, now how many clones do you need to screen.
Discussing the significance of the neurotransmitters within the neurotransmission. Describing a relationship between the dietary fat intake and related health concerns.
A white albino mouse was crossed with a wild grey colored one. The first generation mice were all grey. In the second generation, there were 198 grey and 72 albino mice.
What do you predict would be the main product. How many can the predictions above be used to determine the mechanism by which the fatty acids are released from the fatty acid synthetase.
What advantage will heterozygotes have over the homozygotes. Why selection inevitably favors any gene that is good at getting into the next generation, regardless of whether that gene helps or hurts the ecosystem, the species, the group, or even the ..
At another locus, sepia eyes are inherited as an autosomal (non sex linked) recessive. The presence of the white eye phenotype (x superscript w x superscript w in females, x superscript y in males) masks sepia phenotype, so regardless of the sepia ge..
define how this cell would be activated the cellular and molecular interaction(s), and the various steps and factors leading to an appropriate immune response to clear the toxin.
Compute the surface-area-to-volume ratio of a cube whose sides are 100 um in length to estimated the surface-area-to-volume ratio of the fern spore cell.
Genetic variation in the population of organisms is good; but, sometimes mutations can be harmful or cause genetic disorders.
Which of these statements can apply to endocrine disruptors.
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