User Account

All Pages

You have a 25kd histidine tagged bacterial protein 100 pure

Assignment Help Chemistry
Reference no: EM13644081

You have a 25kD histidine tagged bacterial protein (100% pure). You apply this pure protein to a Ni+2 agarose column. Then you apply a crude mouse cell extract to the column. After extensive washing, you apply an imidazole buffer. Analysis of the resultant elution on an SDS-PAGE gel indicates two proteins (25kD and 75kD) of equal coomassie blue staining. What is the simplest explanation for why the 25kD and the 75kD proteins copurified off the column?

Reference no: EM13644081

Questions Cloud

For the most recent year available the mean annual cost to : for the most recent year available the mean annual cost to attend a private university in the united states was 20332.
A if they move at constant velocity what is the frictional : block a of mass 3.6 kg is on block b of mass 6.6 kg. block b is on a frictionless surface and the coefficient of static
A 01569-g sample of solid magnesium is burned in a bomb : a 0.1569-g sample of solid magnesium is burned in a bomb calorimeter that has a heat capacity of 1769 jdeg c. the
Determine the dimensionless groups using dimensionless : an airplane wing with chord length of 5ft and span of 30ftis designed to move through standard air at a speed of
You have a 25kd histidine tagged bacterial protein 100 pure : you have a 25kd histidine tagged bacterial protein 100 pure. you apply this pure protein to a ni2 agarose column. then
What is the probability that a particular arrival-time : in using the triangulation method to determine the range of an acoustic source the test equipment must accurately
Two 50 g ice cubes are dropped into 200 g of water in a : two 50 g ice cubes are dropped into 200 g of water in a glass. if the water was initially at a temperature of 25oc and
The volume flow rate of the air returned to the workspace : air from a workspace enters an air conditioner unit at 30 degc dry bulb and 25 degc wet bulb. the air leaves the air
If a storm window is installed having the same thickness of : what is the rate of heat loss through a glass window 3.0 mm thick if the outside temperature is -20o c and the inside

Reviews

Write a Review

Chemistry Questions & Answers

  Explain the normal boiling point of a liquid

Define the normal boiling point of a liquid. Explain why atmospheric pressure affects boiling points of liquids.

  State what mass of liquid water will remain when equilibrium

Consider a sample of 3.5g of water, which has an equilibrium vapor pressure of 23.8mmHg at 25.0 degC. IF this sample is placed into a 6.00L flask, what mass of liquid water will remain when equilibrium is established

  How to calculate the reaction rate at the conditions

Consider the decomposition of N2O5 in carbon tetrachloride (CCl4) at 45?C 2N2O5(soln) 4NO2(g) + O2(g) The reaction is first-order in N2O5, with the specific rate constant 6.08 x 10-4/s. Calculate the reaction rate at these conditions.

  Explain what is the partial pressure of each gas

Nitrogen dioxide (NO2) cannot be contained in a pure form in the gas phase because it exists as mixture of NO2 and N2O4. At 25°C and 0.98 atm, the density of this gas mixture is 2.4 g/L. What is the partial pressure of each gas

  Find out the final temperature of the mixture

30.0 mL of pure water at 280. K is mixed with 50.0 mL of pure water at 348 K. What is the final temperature of the mixture? Ignore significant figures for this problem.

  Find out whether each is unimolecular or bimolecular

For the elementary processes whose equations are given below, determine whether each is unimolecular or bimolecular and determine the rate law for each process.

  State three reasons why spectrophotometric analyses used

Give three reasons why spectrophotometric analyses are performed using a narrow wavelength band centered at ? max. Give the purpose of the following commands:

  Explain hydrolysis of an ester

Considering the mixture of the two compounds and range of the melting points, the reason recrystallization was difficult was due to a broad melting point range.

  How many moles of cn- ions will be contained in soln

A 300 mL stock solution contains 0.9 M Ca(CN)2. When 100 mL of the stock solution is diluted to 120 mL, how many moles of CN- ions will be contained in the dilute solution.

  Predict how the ratio of the equilibrium pressures pso2

predict how the ratio of the equilibrium pressures pso2 would change when the. pso3 temperature of the uncatalyzed

  Explain what is the defenition of the word bond

What is the defenition of the word bond, family, group, and periodic table. my teacher told us to define these words. I tried dictionary.com and they dont have anything there. well they do, but not what i am looking for.

  Define what will the volume be if the preassure is increased

7.0and 1.00atm pressure what will the volume be if the preassure is increased at 50.0atm

Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

Submit Assignment

Academics

Major Subjects

Majors

Get In Touch

whatsapp: +1-415-670-9521

Phone: +1-415-670-9521

Email: [email protected]

TERMS & POLICIES

HELP & SUPPORT

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd