You have a 25kd histidine tagged bacterial protein 100 pure

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Reference no: EM13644081

You have a 25kD histidine tagged bacterial protein (100% pure). You apply this pure protein to a Ni+2 agarose column. Then you apply a crude mouse cell extract to the column. After extensive washing, you apply an imidazole buffer. Analysis of the resultant elution on an SDS-PAGE gel indicates two proteins (25kD and 75kD) of equal coomassie blue staining. What is the simplest explanation for why the 25kD and the 75kD proteins copurified off the column?

Reference no: EM13644081

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