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Sickle cell anemia is a disease caused by a recessive mutation in the hemoglobin-beta gene on chromosome 11. Sam has sickle cell anemia. Rufus does not. There are two differences between Sam's DNA and Rufus' DNA as shown below:
Sam's DNA: 5'GCGAGATATATGGAGACT coding
3' CGCTCTATATACCTCTGA template
Rufus DNA: 5' GTGAGATATATGGTGACT coding
3' CACTCTATATACCACTGA template
Find the difference that is not in the promoter. Write out the associated mRNA that will be made for both Sam's and Rufus' DNA from this difference.
What is meant by the term "clone"? Why is it important to obtain large quantities of a particular DNA sequence? List the steps you would take to obtain a clone of a DNA sequence.
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