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Write down the conditions and program of pcr of ns3

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Write down the conditions and program of PCr of NS3 gene of HCV(1900bp).

Note:

Reaction should be in 20uL, melting temperature of forward primer is 62 oC while reverse have 59 oC concentration of MgCl2 should be 2.7mM while concentration of templet DNA is 750ng. Run your amplified product on the agarsoe gel with 10kb ladder and show the bnd

Reference no: EM132621591

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