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phenylalanine medium sometimes is prepared in broth form with a pH indicator simiar to that used in decarboxylase medium so that increases in pH then can be detected by development of purple color. when testing an organism for the ability to deaminate phenylalanine, would you expect to overlay this medium with mineral oil? why or why not?
Glycogen stored in muscles can be mobilized to supply metabolic energy by hydrolyzing individual glucose subunits from the polymer.
The correct folding of proteins is necessary to maintain healthy cells and tissues. Unfolded proteins are responsible for such neurodegenerative disorders as Alzheimer's, Huntington's, and Creutzfeld-Jacob disease.
Insulin and glucagon are hormones that exert their effects on the liver, and you know by now that the responses of the liver to these hormones are exactly the opposite of each other.
Choose the correct definition of metalloprotein. 1. a carrier protein that allows metal ions to diffuse across a cell membrane 2. a co-factor regulating enzyme activity 3. a protein whose function depends on the presence of a specific metal.
You have a cell suspension containing 4 * 10^5 cells/ml and want to seed these cells on microcarriers at a density of 1* 10^4 cells/cm^2. The average diameter of the microcarriers is 157.5um (the diameter range supplied by the vendor is 115-200um)..
Sequencing of a new bacterial genome revealed a circular genome of 2.9 x 106 base pairs. Open reading frame (ORF) analysis indicated the presence of 2,120 ORFs that encode proteins with a median length of 270 amino acids (aa) and an average length..
Discuss how does column chromatography separate molecules such as DNA and RNA? Explain whyis it difficult to separate large amounts of RNA and DNA using gel electrophoresis?
What is the advantage of closed stomata to a plant when water is in short supply? What are the disadvantages?
What is hemophilia and why can females, but not males, be carriers of hemophilia and other X-linked recessive characteristics?
If you grew that mutant in glucose, lactose, IPTG, and glucose and lactose how would the galactosidase activity differ from an E. coli strain containing a functional lac operon?
Assume you start with 10 ml of 2% acetic acid, and you add 90 ml of water. We will call the resulting solution "dilution#1". What is its concentration?
In what way may we be able to take advantage of retrotransposons in human gene therapy? How would this differ from our current use of retroviruses?
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