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Acetylcholine binds to a GPCR on heart muscle, making the heart beat more slowly. The activated receptor stimulates a G protein, which opens a K+ channel in the plasma membrane. Would the following enhance or diminish the effect of Ach?
(a) addition of a high concentration of a non-hydrolyzable analog of GTP(b) mutations in the acetylcholine receptor that weaken the interaction between the receptor and the G protein(c) mutations in the acetylcholine receptor that weaken the interaction between the receptor and acetylcholine(d) addition of a drug that prevents the ? subunit from exchanging GDP for GTP
the required reagents involved in making the PCR work.
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You have a 1mM stock solution of Arsenic to treat culture cells in a liquid medium. You need to treat your cells with Arsenic such that it has a final concentration of 5nM once it has been added to the cells.
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