Reference no: EM133259696

Need typed answer. No handwritten. 

Read this: This unit describes growth and maintenance of Streptococcus pyogenes or the Group A Streptococcus (GAS). These protocols should also be applicable to closely related species of Streptococci such as the Group C Streptococcus (e.g., GCS, S. equi) and Group G Streptococcus (e.g., GGS, S. anginosus) and potentially others. However, the protocols described here are specific for GAS. S. pyogenes is a facultative anaerobe and is grown at 37°C in either ambient air or in 5-10% CO₂. Like all streptococci, GAS is both catalase and oxidase negative. GAS lacks the necessary enzymes for a functional TCA cycle and oxidative-cytochromes for electron transport; therefore, relies completely on fermentation of sugars for growth and energy production. It is a member of the lactic acid bacteria and is homofermentative for lactic acid production from glucose fermentation. Specific components of a rich growth medium for GAS include neo peptone extracts, glucose as carbon source, and a complex mixture of nutrients from beef heart infusion as first described by Todd & Hewitt (Todd and Hewitt, 1932). GAS is considered a multiple amino acid auxotroph requiring nearly all amino acids to be present in its growth media. A Chemically Defined Medium has been developed for GAS containing all of the necessary amino acids for GAS growth (van de Rijn, 1980).

Why would you expect these bacteria to grow very slowly even when grown under ideal conditions (e.g. using the chemically defined medium specifically designed for these bacteria)? Please explain.