Reference no: EM13893898

1.  Fill in the following table and use it to answer the following questions (1pt).

Mannitol concentration (M)	Calculated Osmotic potential of the solution	Drop behavior (rise or drop and how fast)	Change in length of the potato tissue	Change in mass of the potato tissue
0.05	-0.122	Sinking slowly	1	0.22
0.1	-0.243	Sinking verly slowy	0	0.21
0.2	-0.487	Sink(slow)	1	0.03
0.3	-0.730	Sink(mid up speed)	0	-0.03
0.4	-0.974	Rise (slow)	-1	-0.32
0.6	-1.461	Rise(mid up speed)	-1	-0.47
0.8	-1.948	Rise (fast)	0	-0.71
1.0	-2.435	Rise (fast)	0	-0.87

2a. Graph the change in weight versus the concentration of mannitol . Draw on the graph how you would estimate the water potential of the tissue.

2b. Graph the change in length versus the concentration of mannitol. Draw on the graph how you would estimate the water potential of the tissue.

3. From your graph what do you estimate the water potential of the potato tissue to be?

4. Does the increase in tissue weight and length resulting from gaining water in the less negative water potential solutions eventually plateau and reach a maximum? Why? Explain this observation based on what we know about the two potential terms that contribute to total water potential in a plants cell or plant tissue.

5. Why would the potato tissue shrink when it loses water (explain this in terms of pressure potential?

6. If you were using this technique with a tissue with very rigid, inelastic cell walls (such as a woody tissue) which measurement method would be least effective? Why?