Why was a restriction enzyme added to the two dna samples

Reference no: EM133477112

Problem

Based on earlier techniques developed by Susumu Tonegawa, who was recognized as sole recipient of a Nobel Prize in 1987, a paper published together with Nobumichi Hozumi was pivotal to opening up the understanding of how there can be diverse combinations of antibodies. Answer the following questions describing the experiments related to determination of antibody diversity. (See Classic Experiment Box?6-1:?DNA Recombination of Immunoglobulin Genes Occurs in Somatic Cells in Kuby Immunology.)

Question I. What two sources of cells were used?
Question II. Why was a restriction enzyme added to the two DNA samples?
Question III. Why were these samples subjected to agarose gel electrophoresis?
Question IV. After extraction of DNA from the agarose gel, what two probes were added to hybridize to the DNA fragments?
Question V. Why were these two probes used?
Question VI. Describe the differences in the size of DNA fragments plotted for each cell population.
Question VII. What conclusions did the researchers reach from these differences?

Reference no: EM133477112

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