Why use tritiated thymidine and not some other nucleotide

Reference no: EM13153513

A)In a classic experiment, cells were treated with tritiated thymidine and fixed immediately. The cells were then treated for autoradiography and then examined in a microscope. What the researchers were looking for was the timing of DNA synthesis. First, why use tritiated thymidine and not some other nucleotide?

B)In this experiment described above, the researchers looked specifically for mitotic figures, that is cells actively undergoing mitosis. They saw no radioactive mitotic figures. Why not?

C)How would you do the experiment described differently so that labeled mitotic figures would be observed?

Reference no: EM13153513

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