Why the banding patterns from lane 1-6 in region z looks

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Reference no: EM133255884

Case: You want to identify the DNA binding site of a suspected Transcription Factor. You performed a DNA footprint, and the DNA sequencing gel result is shown below. Lanes A, C, G and T are the DNA sequencing ladder. The red lines only indicate the presence of a base but are not representative of its location. Refer to the sequence provided on the left as your guided

a. State the sequence of the TF binding site (coding strand 5' to 3').

b. Why did you choose the site mentioned in 5a?

c. What is the most likely identity of lanes 1 and 6?

d. Justify whether this experiment is based on Sanger or Maxam-Gilbert sequencing method and if either one of the methods can be used for DNA footprinting.

e. Why the banding patterns from lane 1-6 in region Z looks similar?

Reference no: EM133255884

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