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1) Why is the Gram stain called a differential stain?
2) What color is a gram negative organism at the end of a Gram staining procedure? A Gram positive organism?
3) What is the most important step in the Gram staining procedure? Why?
4) What is the purpose of the 95% ethanol step in the Gram staining procedure? The Gram's iodine?
5) Explain the use of a Durham tube.
6) In the PR broths you did last week, the pH indicator was ______________________________.
7) In the presence of acid production, what color does it (the pH ind. above) turn? ________________________.
Gas is detected in PR broths tubes by a ________________________________.
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