Why is proper pipetting important in a microbiology lab

Assignment Help Other Subject

Reference no: EM133582265

Assignment: Pipetting

Learning Objectives

1) Select the correct micropipette for its purpose.
2) Use the two stops of the pipette.
3) Explain pipetting techniques.
4) Perform a serial dilution.
5) Quantify the protein content in a sample with a Bradford assay.

Introduction: Master pipetting virtualsimulation lab

In the Pipetting simulation, you will determine the amount of essential amino acids in genetically modified corn. Using a micropipette, you will prepare a serial dilution, and measure the protein concentration with a Bradford assay.

Get to know the micropipette

You will be introduced to the basics of pipetting as you dive right into the experiments. Step by step, you will learn how to adjust the volume, use the two ‘stops' of the pipette, and find out which pipette to use for which volume range. You will also learn tips and tricks to make your pipetting more accurate.

Perform a serial dilution

Once you master the art of pipetting, you will dilute your protein solution 10.000 fold! While you progress through the lab, the technique will become more routine. After this mission, you will understand the benefits of serial dilutions, and be able to perform one by yourself.

Measure the protein content in the sample

In this mission, you will dye your proteins blue, and use your new skills to prepare a microplate for a Bradford assay. In this colorimetric assay, you shine light of a specific wavelength through the plate, and measure the absorbance in each well. Finally, you will compare the absorbances of your sample to a sample of known protein concentration.

Will you be able to calculate the amount of essential amino acids in the corn cob you collected?

Task

Question A. Purpose: Why is proper pipetting important in a microbiology lab?

Question B. What are the three different types of pipettes that are commonly used in lab?

Question C. List 4 safety features that you will need to follow when using pipette?

Question D. Reflection: Write 5 sentences on what you learned from this simulation. What did you like and what was something that you would prefer not be a part of this simulation?

Reference no: EM133582265

Questions Cloud

What diagnosis appears to be present and who would be : Sammy is doing really well in his new foster home and has no current health conditions. What diagnosis appears to be present and who would be diagnosed with it?

What is the fallacy in the passage : What is the fallacy in the passage? "Sally, please go to the prom with me. It'll crush my mother if you don't say yes, and she has a weak heart

What do you think it means to be an active and informed : What do you think it means to be an active and informed listener when it comes to understanding a topic and forming your opinion on that topic

Is the doctor correct and is there more to the story : Knowing that you are studying the immune response, he is calling to ask you to explain this statement. Is the doctor correct? Is there more to the story?

Why is proper pipetting important in a microbiology lab : Why is proper pipetting important in a microbiology lab? What are the three different types of pipettes that are commonly used in lab?

Identify and explain why the situations are examples : Identify and explain why the situations are examples of good, bad, or ugly experiences on the Internet: You have e-mailed a leading authority

What type of context clue is embedded in the sentence : Nancy has tried to eradicate the ants in her kitchen, but no matter how often she sprays, they soon reappear. What type of context clue is embedded

Webers rationality and concrete examples : Be sure to use concepts from lectures, including Weber's rationality (as best you can), AND concrete examples from the book with page numbers in your response

What are some reasons or benefits of using and carefully : What are some reasons or benefits of using and carefully analyzing the best sources available for your Informative Speech?

User Account

All Pages