Why is naoh required for biuret reagent to test for proteins

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Assignment: Cells and Macromolecules

1. Match the appropriate definition with the corresponding types of bonds:

____ Ionic bonds              A. Formed by electrostatic attraction between a proton and electronegative atom in the other
____ Covalent bonds        B. Formed by sharing of electrons between 2 atoms
____ Hydrogen bonds       C. Occur between atoms of opposite charges

2. Match the appropriate macromolecule with its definition:

____ Proteins              A. Made of C, H, and O; mono-, di-, or polysaccharides
____ Carbohydrates     B. Compounds that are insoluble in water but soluble in solvents
____ Nucleic acids        C. Compounds made of amino acids and bound by peptide bonds
____ Lipids                 D. Compounds made of a phosphate group, sugar, and nitrogenous base that function in encoding, transmitting and expressing genetic information

3. True or False: Inorganic molecules never contain carbon and organic molecules always contain carbon.

4. Orange juice is acidic. Therefore:

Its pH is _________________________ (less than or greater than) 7.

5. After adding HCl (hydrochloric acid) to the buffered solution (sodium bicarbonate), the pH:

a. Decreased
b. Increased
c. Remained the same

6. After adding HCl to the unbuffered (water) solution, the solution became acidic. After adding Tums to this solution, the pH:

a. Decreased
b. Increased
c. Remained the same

7. Why is NaOH required for the Biuret reagent to test for proteins?

a. To make a water by-product
b. To make the solution basic
c. To make room for the Biuret to bind
d. All of the above

8. Disaccharides have

a. One monomer
b. Two monomers
c. Multiple monomers

9. What substance can we add to a solution of water and oil in order to dissolve the oil in water?

a. Alcohol
b. Biuret reagent
c. Benedict's solution
d. Emulsifier.

Reference no: EM131522576

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