Why is it important that the bacteria are in log phase

Reference no: EM133576167

1. Why is it important that the bacteria are in log phase? In log-phase growth, it creates a dense, turbid layer of bacteria able to sustain viral growth.

2. What is the control?

3. What do you expect the control plate to look like and why?

4. What is the ideal plaque count range?

5. Which dilution(s) yielded results in the ideal range

6. You have performed a serial dilution of phage, incubated 100 l of the phage dilution with E. coli below is your plaque count. Using the formula from the question above, determine the PFU for each dilution and the concentration of the stock solution.

Plaque count Viral dilution PFU/ml 950 10^-4 11 10^-6 100 10^-5

7. What are 3 differences between a bacteriophage and a bacterium?

8. How could you easily determine in the laboratory if the phage was lytic or lysogenic?

9. What is a serial dilution? Draw/describe a serial dilution of 625-fold from a stock viral solution. You will perform the dilution in 4 steps. The total volume of each tube in the series is 500 ul.

what is your dilution factor? 1:.....?

volume of dilution:.....?

volume of virus:....?

10. How might phage be used as a treatment for a bacterial infection?

Part II- Phage Transduction

Watch the phage transduction video and answer the following questions:

Do phage particles enter the cell during viral replication?

How does transduction contribute to bacterial genetic diversity?

Why do phage package host DNA?

How do you select for transduction?

Reference no: EM133576167

Questions Cloud

May Ed successfully sue Juan for damages : Explaining that he had already sold the car to Boy. May Ed successfully sue Juan for damages?

What happens at the end of mediation : What happens at the end of mediation? Labor lawyers for management and the union reach a bargaining agreement between management and the union.

Describe mechanisms of hormonal feedback regulation : Describe the mechanisms of hormonal feedback regulation, including negative and positive feedback loops,

Genetic abnormality present in the patient sample : You will be asked to choose the genetic abnormality present in the patient sample.

Why is it important that the bacteria are in log phase : Why is it important that the bacteria are in log phase? In log-phase growth, it creates a dense, turbid layer of bacteria able to sustain viral growth.

Enzyme immunoassays from other virus detection methods : Distinguish the purpose of enzyme immunoassays from other virus detection methods. They are used as a confirmatory test.

Difference in structural consequences for nucleosomes : Contrast the difference in structural consequences for nucleosomes, associated proteins,

Adult stem cells are more expensive to harvest : Why do embryonic stem cells have greater medical potential than adult stem cells? Adult stem cells are more expensive to harvest.

Hemolysin-leukotoxin-exfoliative and exotoxin : Hemolysin, leukotoxin, exfoliative, Exotoxin and endotoxin Pick one of the toxins from the previous question and explain it in more detail.

User Account

All Pages