Why is it a good idea to treat a protein

Reference no: EM13117983

Iodoacetate reacts with cysteine side chains in proteins to form S-carboxymethyl derivatives. Why is it a good idea to treat a protein first with beta-mercaptoethanol before carrying out carboxymethylations of cysteine residues? Why might treatment with urea also be useful?

Reference no: EM13117983

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