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1. Explain your evidence for coming to this conclusion. Is this sufficient evidence to convict the suspect of the crime? Why or why not?
2. It what way did this lab simulation differ from what is actually performed in a forensic analysis?
3. Why is restriction fragment length polymorphism (RFLP) commonly refered to as a "DNA fingerprint"?
4. A restriction endonuclease "cuts" two DNA molecules at the same location. What can you assume is identical about the molecules at that location?
5. Do any of your suspect samples appear to have EcoRI or Pstl recognition sites at the same location as the DNA from the crime scene? If so which suspects and what are the band lengths (bp) that match?
6. Why is DNA sometimes refered to as a palindrome?
In a 1973-74 situation comedy All in the Family, the character Archie Bunker says, Let me tell you something about beer, You can never buy beer, you can only rent it.
The final elution of your miniprep plasmid DNA has a concentration of 250 ng/uL. You need to load 500 ng on an agarose gel to be able to see the DNA.
Explain why does nitrogenous waste build up in cellular fluids? Discuss the tradeoffs between water loss, energy efficiency and toxicity with the excretion of ammonia, urea, and uric acid.
Describe why it may be too simplistic to label disease-causing alleles as "bad" and corresponding "normally" functioning alleles as "good."
You perform a forward genetic screen to look for Arabidopsis thaliana mutants that are altered in cold stress responses. You find two mutants with opposite phenotypes.
Provided the total population size of the population in Table 2 (in year 2012), using the per capita growth rate in question five, and assuming that this growth rate does not change,
A 23 year old medical student has mixed venous oxygen and carbon dioxide tensions of 40 mmHg and 45 mmHg, respectively. A group of alveoli are not ventilated in this student because mucus blocks a local airway.
In supine (laying down) position, a human being's heart rate is elevated to (105 beats/min). Why? Why human being's heart rate is even more elevated to (135 beats/min) in upright (standing) position? Explain the mechanism here.
While working in a cell biology laboratory, you are instructed to create a solution that is iso-osmostice to the cells that the lab uses.
An enzyme that follows Michaelis-Menten kinetics has a Km of 1 ?M. The initial velocity is 0.1 ?M min -1 at a substrate concentration of 100 ?M. What is the initial velocity when [S] is equal to 2 mM?
Write a one-page essay on Biotechnology using recent articles either in print or web-based. Please list the sources used for this.
During mitosis it is important for sister chromatids to stick together (called "cohesion") during some stages and then separate. Researchers have identified proteins called "cohesins" that are involved in sister chromatid cohesion. If you wanted t..
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