Why is a dna primer required to generate a probe

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  1. Why is a DNA primer required to generate a probe? 
  2. Why does absorbance at 260 nm increase as a DNA molecule denatures? 
  3. Are all transgenes involved in gene silencing? Explain. 
  4. You are studying factors that increase an enzyme's activity and must determine the controls for the assay. Explain your thinking for the selection of a negative and positive control
  5. Explain what does it mean by a qualitative and a quantitative assay?
  6. Differentiate between a mass ruler and a DNA ladder.
  7. A PCR can be repeated many times to maximize the number of products made. What are the benefits and drawbacks of having a high number of cycles? 
  8. What features would you look for to identify an ORF in a transcript?
  9. During plasmid isolation, provide a situation when you collect the supernatant and another situation when you collect the pellet?

Reference no: EM133476269

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Why is a dna primer required to generate a probe : Explain what does it mean by a qualitative and a quantitative assay and Differentiate between a mass ruler and a DNA ladder
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