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Question - You have just graduated from the University of the West Indies and have applied to an international biotechnology company. As part of their prescreening process they send you a tutorial which reads:
In your first week of work, your benchmate approaches you with a problem. Using a site-directed mutagenesis kit, she has been generating mutants of the enzyme lysozyme. One of her mutants (Glu35 to Ala35 ) shows virtually no activity, while two others, (Asp52 to Phe52) and (Asp101 to Gly101 + Trp62 to His62 ) show reduced activity compared with the wild type enzyme. She is concerned about the lack of activity of these enzymes. Explain to her, as thoroughly as you can, why her mutants probably behave as observed. Further, outline what modifications you might suggest making to the wild type enzyme to enhance activity.
Calculate the pH of each aqueous solution: (a) 0.0066 MHCl; (b) 0.000012 M KOH. Calculate the value of [-OH] from the given [H3O+] in each solution and label the solution as acidic or basic.
Given the following information for element X, identify the element group in the periodic table: the electron affinity is positive; the 1st ionization energy is less than the 2nd and 3rd ionization energies and is much less than the 4th ionization..
What weight of glucose in grammes is required to make 675 ml of a 750 mM solution. Glucose Mr = 180.16
a 55.0 gram piece of ice at 0.0 c is added to a sample of water at 8.0 c. all of the ice melts and the temperature of
The concentrations of Al and K in a sample of riverwater are 0.50 mg/kg and 1.3 mg/kg, respectively. Express the concentration in molality?
what is the theoretical yield for carbon dioxide. if 1.44 g of carbon dioxide is isolated from this reaction, what is the percent yield for carbon dioxide
Super-green is an indicator with a Ka of 1.6 × 10-7. The acid form is yellow and the base form is blue. Awesome-orange is an indicator with a Ka of 5.0 × 10-5.
0.40 moles of an unknown acid (HA)is added to 600 ml of pure water. The pH of the resulting solution is 3.00. What is concentration of A- in the solution. What is the Pka of the acid
Given the reaction 2C2H2(g) + 5O2(g) ->4CO2(g) + 2H2O(g) , how much oxygen gas is needed for the complete combustion of 167 g of acetylene (C2H2)? Answer in units of g
An organic acid contains 41.4% carbon, 3.47% hydrogen, and 55.1% oxygen by weight. A 0.080-mol sample of this compound weighs 11.60 g. What is the molecular formula of this acid
Using the weak acid that you were assigned in this experiment and .100M NaOh, describe exactly (show calculations) how you would prepare 100.0mL of a buffer at pH = 5.50
honda developed a car that utilize a hydrogen fuel cell to power the car instead of gasoline. the estimated mileage for
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