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In his studies of glucose metabolism by yeast, Louis Pasteur discovered that the sudden addition of oxygen (O2) to a previously anaerobic suspension of yeast in grape juice resulted in a dramatic decrease in the rate of glucose consumption. This “Pasteur effect†can be counteracted by the addition of 2,4-dinitrophenol (DNP), an uncoupler of the mitochondrial electron transport chain. (a) Why do yeast cells consume less glucose in the presence of oxygen? Can you estimate how much less glucose they use in the presence of oxygen? (b) Why does DNP counteract or prevent the Pasteur effect?
Assume you are walking on the beach when you step on a shell and cut your foot. 4-cells of Vibrio vulnificus bacteria are transferred from shell and deposited deep inside the cut.
Using the understanding of osmosis describe, why putting salt on a pork chop before cooking it on a grill is probable to result in a dry tough piece of meat.
Briefly explain why hydrogen bonds and salt bridges do not contribute much to protein stability at room temperature.
Describe what you observed in your cheek smear wet mount, direct stained slide, and indirectly stained slide. What were similarities and differences. Describe what you observed in your yeast wet mount, direct stained slide, and indirectly stained sli..
Compute the number of RBC if the dilution is 1:50, the cell count is 400 cells counted in five (5) RBC squares of the hemocytometer.
What occur on BTB solution if you combine into germinating radish seed? And what happen if BTB solution combines into yeast glucose solution.
Discuss how many molecules of histone 2A would be required in a typical human cell just after the completion of S phase, assuming an average nucleosome spacing of two hundred bp?
Assume you are provided a solution of 5mM benzoic acid at pH 8. You determine the absorbance to be 2.9 ×103. You lower the pH to 3 with 100% acetic acid.
Since the invention of the first vaccine in the 1700s, there has been controversy whenever a new vaccine is introduced. Are they safe explain your answer.
A patient has a stenotic mitral valve which has a reduced effective orifice area of 1.0 cm^2. The systolic ejection period is 330 milliseconds with a heart rate of 70 beats/min.
A population is reproductively isolated. Determine which of the following is primarily responsible for the introduction of new alleles at genetic loci into the population?
About 13 different species of finches inhabit the Galápagos Islands today, all descendants of a common ancestor from the South American mainland that arrived a few million years ago.
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