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Why do you use naoh and sds for isolating plasmid dna

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Reference no: EM132520127

1. You have five test tubes and they have glucose, sucrose, maltose, trypsin and starch. The technician who made it forgot to label it. Your professor wants you to figure out which tube contains which solution and label it accordingly. You have to determine what tests to do so you can figure out.

2. You are isolating plasmid DNA from bacteria

  • When you take the bacteria culture centrifuge the first time, which part do you take for further use? The pellet or the supernatant. Why?
  • After treatment with neutralization buffer and centrifugation what part do you take? The pellet or the supernatant. Why?

3. Why do you use NaOH and SDS for isolating plasmid DNA?

Reference no: EM132520127

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