Why do we conjugate secondary antibodies

Reference no: EM133476236

Why do we conjugate secondary antibodies but not primary antibodies with a molecule that can be detected? Name 2 reasons.
You would like to purify a negatively charged protein from a mouse cell extract. Which chromatography technique can you use and why? Explain your reasonings and include what reagents or chemicals you would use which is specific for your protein to carry out the purification procedure.
When do you need gold-conjugated secondary antibodies for protein detection? Explain.
Will E. coli take up linear DNA upon electroporation?
Why do you need an open-ended vector for molecular cloning and how do you generate the open-ended form from a closed circular vector?
Why can't you use CaCl₂ to make electrocompetent cells?
Why are BACs and YACs usually single copies?
Can you have a multiple cloning site in the ori of a vector? Why?

Reference no: EM133476236

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