Why do we conjugate secondary antibodies

Assignment Help Chemistry

Reference no: EM133476236

Why do we conjugate secondary antibodies but not primary antibodies with a molecule that can be detected? Name 2 reasons.
You would like to purify a negatively charged protein from a mouse cell extract. Which chromatography technique can you use and why? Explain your reasonings and include what reagents or chemicals you would use which is specific for your protein to carry out the purification procedure.
When do you need gold-conjugated secondary antibodies for protein detection? Explain.
Will E. coli take up linear DNA upon electroporation?
Why do you need an open-ended vector for molecular cloning and how do you generate the open-ended form from a closed circular vector?
Why can't you use CaCl₂ to make electrocompetent cells?
Why are BACs and YACs usually single copies?
Can you have a multiple cloning site in the ori of a vector? Why?

Reference no: EM133476236

Questions Cloud

Discuss multiculturalism within your chosen country : Discuss multiculturalism within your chosen country. Discuss how tradition has impacted the development of the legal system.

Describe the current business strategy and model : Define dynamic capabilities, using any of the definitions you found in this unit's readings. Cite the source, including the page number where you found

Describe the experiment, mention the steps from start to end : Describe the experiment, mention the steps from start to end. Steps like washing, etc. are not required. In addition, mention any controls needed to improve you

What are considered professional and unprofessional attire : CS 212- Prepare a 7 to 10 slides PPT that explains what are considered professional and unprofessional attire and behaviors for your chosen career.

Why do we conjugate secondary antibodies : Why do we conjugate secondary antibodies but not primary antibodies with a molecule that can be detected? Name 2 reasons

Explain how and why scientists make generalizations : Explain how and why scientists make generalizations. Include all the following elements in a clearly organized manner in your essay (but probably not

Discuss about white fragility : How can you take one idea from the presentation/discussion about white fragility and apply it to working with children, families, or communities?

How one innovation has affected you at work, home or school : how one innovation has affected you at work, home or school. Explain how that innovation has changed over time and how it has altered the way you complete

What ph would all of the n-terminal residues : What pH would all of the N-terminal residues on the peptide (C-A-V-S-E-F) have fully protonated N-termini and 60% of their side chains deprotonated

User Account

All Pages