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Unlike microscopy, cell fractionation and protein purification is often performed as a series of linked steps that can often take several days or longer than a week's time.
a. Most buffer solutions used in this process are isotonic and contain protease inhibitors. Why would this be the case?
b. EGTA and some types of protease are commonly used for cell dissociation. Why are each important and why do some cells dissociate from each other more easily than others?
Look up the genome size of the following organism: Escherichia coli, Homo sapiens, Zea mays, Li Hum longiflorum, Protopterus aethiopicus.
What is the value for the concentration of calcium in the cytoplasm of most of your cells.
What sort of signalling occurs when insulin is secreted from the pancreas and acts on muscle cells to increase glucose uptake.
The antibiotic Gramicidin A can transport Na+ ions into a certain cell at the rate of 5.0 x 10^7 Na+ ions channel^-1 s^-1. Calculate the time in seconds to transport enough Na+ ions to increase its concentration.
The body’s oxidation of glucose is commonly described as the burning of fuel. Is this analogy correct? Explain your answer.
After injecting glucose into the E. coli culture, there is a delay before the rate of oxygen consumption increases. What is causing this delay?
Think about the following two unlinked genes in the poisonous plant, Jimson Weed. The dominant allele V produces violet petals while the recessive allele v produces white petals.
Assume if you have flown in a commercial airliner, you have heard the flight attendants instruct you that in case of a sudden loss of cabin pressure, oxygen masks will be deployed.
The phosphorylation and dephosphorylation of proteins is a vital means of regulation. Protein kinases attach phosphoryl groups, whereas phosphatases remove phosphoryl group from the target protein. What is the energy charge of this kind of covalen..
With these anatomical features in mind, do some research in order to determine an angiosperm which has modified leaves, stems, roots or flowers that do not function in the normal manner, or that function in an unusual manner.
In 1918, the tsar and tsarina of Russia and their five children were presumably assassinated and buried in an unmarked grave. Later, several women came forward and claimed that they were in fact Anastasia.
Determine the realitive risk and odds ratios for a child's diagnosis of ADHD relative to exposure to lead paint, stratified by the father's diagnosis with and without ADHD.
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