Reference no: EM133443227

Endospore Stain Assignment

1. Why do bacteria produce endospores? What is the function of this structure? Where is this structure located with respect to cellular envelope?

2. What is the medical importance of knowing that a particular bacteria species produces endospores? What precautions must be taken when working with endospore forming bacteria?

3. How are endospores detected?

4. Briefly explain the endospore stain procedure. Do not just list step by step procedure. It is more important to know why each step is performed. Example: Why do we use malachite green? What does it stain and how does it help us visualize the spores?

5. What type of staining technique is it?

6. Why do we "steam" the malachite green stain?

7. Do all bacterial cells stained using this technique show endospores?

8. How would you know if it is a "true" spore former vs a non-spore forming bacteria? What would you see in your slide?

9. From our list of 80 pathogens, identify and list 5 bacterial pathogens that produce endospores.

Do not select the same bacteria from the other lab writing assignments

10. From the 5 spore forming bacterial species you selected, pick 1 and explain bacteria's effects to the body (patient). Example: describe the signs/symptoms of this bacterial infection.