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The Meselson-Stahl experiments were groundbreaking studies in molecular biology. Some of the clarity of the centrifugation results was due to the that the DNA was fragmented during handling (although the experimenters did not know it at the time). As quoted in Davis, Stahl said that pipetting DNA was like "throwing spaghetti over Niagara Falls." When DNA breaks into fragments, the sugar phosphate backbone is broken, forming many short double helixes. Why did the fragmented DNA still show the same pattern of nitrogen density banding?
Explain how a mutation could inhibit the binding of sigma factor to DNA. Describe two specific base substitutions you think would inhibit the binding of sigma factor.
Draw the metabolic pathways for Glycolysis, the Krebs cycle, and the electron transport chain. Indicate at the right the total number of ATP and NADH produced or consumed.
IgM is the first antibody at initially has higher rate during the primary antibody response, why is membrane filter technique useful for a sanitarian working in the filed?
If a 100X solution is 1 M, what is the concentration of a 1X solution? Explain how you would prepare 100 mL of a 0.5 percent w/v agarose in 1X TAE buffer starting with a 20X TAE stock solution and solid agarose powder.
Assume you have a bacterial suspension and you need to dilute it to .0010 of the initial concentration so that you can determine thenumber of colonies per milliliter.
Discuss and explain how ideas about stress immunization evolved into realization that maternal behavior actually plays an important role in mediating the effect of early stress, citing both animal and human research.
During the method of inspiration, the ______ contracts, pulling downward. Also, the ______ muscles contract and lift the rib cage. Together, these muscle contractions result in the ______ in the pressure in the ______ cavity.
What is the difference between an intermediate host and a definitive host? Identify each host in the life cycle of the causative agent of malaria?
For this exercise you were given known phenylalanine deaminase positive and phenylalanine deaminase negative culture. what is the purpose of the uninoculated control? What other types of controls would be useful to increase reliability of the test..
A couple, George and Lara, have three sons. The oldest and youngest are normal but the middle child has xeroderma pigmentosum.
Epigenetics refers to the modification of genes that affect the DNA sequence itself.
What other diseases are thought to be caused by the point mutations? What do point mutations do in those diseases.
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