Reference no: EM132739711

Flow cytometry:----how would the forward and side scatter of a neutrophil compare to that of a lymphocyte?
what information does forward scatter provide regarding a sample being analyzed by flow cytometry?

what information does side scatter provide regarding a sample being analyzed by flow cytometry?

-why is cell sorting using a flow cytometer of value?

-a cell being run through a flow cytometer after being labeled with two antibodies, each conjugated to a different fluorophore. How does the flow cytometer instrument detect both of the fluorophores?

-the x-axis of a flow cytometry dot plot is labeled CD86-PE and the y-axis is labeled TLR4-Alexa Fluor 488. What do these axes labels tell you about the samples that are being analyzed?

-dot blots are typically divided into four quadrants for data analysis. Be able to describe why the presence of a dot in each of these quadrants tell you about the cell that the dot corresponds to?

-if numerous dots are present in one quadrant compared to another, what does that data tell you about the sample being analyzed? be able to interpret the data in a variety of dot plots

Western Blotting:

-in the Western blot technique, why are proteins first run on an SDS polyacrylamide gel?

-why are molecular weight markers included in the gel?

-why are protein samples treated with SDS and mercaptoethanol prior to electrophoresis?

-how are proteins transferred from the gel to a protein-binding membrane such as nitrocellulose of PVDF?

-what roles are played by the primary and secondary antibodies in a Western blot protocol?

-a researcher forgot to block the membrane prior to incubating it with antibodies. At the end of the Western blot protocol, what would the blot look like?

-why is being able to quantitate the amount of protein detected in a Western blot useful?