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1. How are microsatellite markers scored (what are the alleles)?2. Why are microsatellites codominant markers?3. What are the advantages and disadvantages of using RFLP, AFLP, and microsatellite markers? If you don’t know any sequence information for the organism, which marker do you want to use?4. How does the modern Sanger sequencing technique work? What is the purpose of the ddNTPs? How is the order of nucleotides determined?5. What does a sequencing reaction contain?6. How does a cloning vector allow us to sequence an unknown segment of DNA?7. What are the advantages/disadvantages to Roche 454 Pyrosequencing and Solexa Illumina sequencing over the Sanger method?8. Which method was used to generate the first human genome? How much did it cost?9. How much did it cost to sequence the whole human genome using the HeliScope Sequencing System?
In wild petunias, the allele for red flower color ( R ) is dominant over the allele for white flower color ( r ). We sample a population and find 225 plants have white flowers and seventy-five have red flowers.
Helminths are included in the study of parasitology but only one form of the worm is actually parasitic. Discuss the lifecycle of one helminth and state which form of a worm is parasitic larva or the egg.
Carbonic anhydrase has a molecular weight of 30,000g/mol and a turnover of about 1 million per second. If you are given a solution saturated with CO2 containing 2 micrograms (ug) of carbonic anhydrase.
What is hydrogenase? How can this enzyme be used as part of a strategy to "improve" a Rhizobial strain used to inoculate legumes? Describe logic and provide the appropriate diagram.
Discuss the two main classes of T cells and explain how are they distinguished from each other. What are their most important functions.
Sperm cells bind to an egg via ZP3, a glycoprotein in the glycocalyx of the egg, and can be inhibited from doing so by adding an excess of free oligosaccharide. You are interested in finding the protein on the sperm cell responsible for binding to..
A recipe calls for 10 ml of a 25mM stock of EDTA. However, all you have on hand is a five hundred mM stock. How much more concentrated is what you have relative to what the recipe calls for?
How many mls of 0.1 M NaOH would be needed to neutralize completely 250 ml of the 0.2M HA solution?
Before this module evaluation you were very nervous. Consider a snack might help, you ate a big bowl of pasta and some bread a couple of hourse before you started this quiz.
3rd-base wobble allows some tRNAs to recognize more than one mRNA codon. Determine the minimal number of tRNA molecules necessary to recognize the following amino acids?
Discuss how does evaporative cooling contribute to the stability of bodies of water while also preventing from overheating?
A 54-year-old man with a long- standing diagnosis of essential hypertension is meeting with his physician. The patient's physician would anticipate that which of the following phenomena is most likely occurring?
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