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a) Regarding HPLC, explain specifically why it is vital that the mobile phase (Carrier) is either held under a slight vacuum or is purged with helium gas before entering the HPLC column.
b) An analysis of fatty acids is desired. These fatty acids have boiling points in excess of 275O C. The sample is extracted with methanol to obtain a solution that only contains the fatty acids. Which of the following Methods/Cases is not possible and why, & which is the best approach & why?
Case I-The methanol extract is mixed with a compound that reacts with the fatty acids and adds a methyl group to the end of the fatty acid "chains". The result is that the resulting products have drastically lowered boiling points (between 140oC & 210oC).
This modified extract is then injected into a GC under Isothermal conditions with quantification using a Thermal Conductivity Detector.
Case II-The original methanol extract is injected into an HPLC with a water/acetonitrile mixture as the mobile phase. It is varied from 10% acetonitrile to 40% over 15 minutes and a Refractive Index Detector is used.
Case III-Similar to the first Case, except an FID detector is used with Temperature Programming.
Calculate the fraction of space that is occupied by packing spheres in the (a) simple cubic, (b) body-centered cubic, (c) face-centered cubic (close packed) structures
Calculate the osmolarity of this solution. my answer is 0.499 osm. Is that correct? 2*(.1249) + 1 (.2499)
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The glass electrode used in commercial pH meters gives an electrical response proportional to the concentration of hydrogen ion. To convert these responses to a pH reading
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Consider the reversible reaction: A(g) 2B(g) At equilibrium, the concentration of A is 0.381 M and that of B is 0.154 M. What is the value of the equilibrium constant, Keq?
If a 50.0 g object needs 1,145 Joules to increase its temperature by 10.0 oC, what is its specific heat capacity?
As of 2007 world record for swimming 1500.0 m in a long course pool is 14 min 34.56 s. if we follow this rate then calculate how many seconds would be taken by a man to swim 0.850 miles?
If 69.5 kJ of heat is applied to a 1025 g block of metal, the temperature of the metal increases by 11.4°C. Calculate the specific heat capacity of the metal in J/g°C.
Density of liquid methanol, Determine the density of liquid methanol in (lb/ft^3) and convert heat capacity, thermal conductivity and viscosity from SI units to English Units.
suppose 2.8 moles of methane are allowed to react with 5 moles of oxygen? what is the limiting reagent?How many moles of CO2 can be made from this mixture? How many grams of CO2 would produce?
Calculate the Mn of the polyisobutene if A 7.5g polymer sample found to discolor 3.4 mL of a 0.05 M solution of Br in CCl4 ?
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